analysis of the methylome of human embryonic stem cells employing methylated dna immunoprecipitation coupled to next generation sequencing
báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... are names of human embryonic stem (hES) cells lines P denoted the number of passages of the cell lines H9-EB denoted embryoid body (EB) prepared from cell line H9 and the day indicates the time ... Loss -of- function of the miR-17-92 cluster resulted in smaller embryos and immediate postnatal death of animals [67], which could due to the deficiency of their roles in the development of the ... hES-specific miRNAs and their target mRNAs expression level as a whole in human embryonic stem cells These results will help to unravel the biological signalling pathways of hES cells Results It has...
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Báo cáo y học: " GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silico dissection of mixed phenotypes" docx
... Endothelial cells derived from human embryonic stem cells Proc Natl Acad Sci USA 2002, 99:4391-4396 Choi K: The hemangioblast: a common progenitor of hematopoietic and endothelial cells J Hematother ... of factors in the generation of FLK- and SCL-expressing hematopoietic and endothelial progenitors from embryonic stem cells Development 2004, 131:2749-2762 Lacaud G, Gore L, Kennedy M, Kouskoff ... distinct colonies and analysis of gene expression in these colonies might serve to determine whether human BCs posses the potential to differentiate into hematopoietic, endothelial and cardiomyocyte...
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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
... vegetative cells retain the ability to revert into a totipotent state Definition of a stem cell Three basic categories of cells make up the human body viz., germ cells, somatic cells and stem cells ... cells include the bulk of the cells that make up the human adult and each of these cells in their differentiated state has its own copy or copies of the genome with the only exception being cells ... somatic stem cells can be found in diverse tissues and organs The most common adult stem cells are the hematopoietic stem cells, mesechymal stem cells and neuronal stem cells Adult stem cells have...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... of ATP in the dynamics of the actin filaments of the cytoskeleton Biol Chem 2006;387(4):401-6 Lopez LA, Bertini F The in vivo effect of low body temperatures on the degree of polymerization of ... as to enable some of the cells to undergo mitosis Mitotic hESC were then arrested at metaphase by colcemid treatment (0.1 μg/ml) for 16-18 h Subsequently, the cells were subjected to hypotonic ... Virtually all of the detached cells were determined to be non-viable with tryphan blue staining (data not shown) All of the detached cells were washed off prior to the MTT assay From the raw absorbance...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... methylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3; H3K36me3, trimethylation of lysine 36 of histone H3; MacroH2A, ... expressed in the cell type of origin used to derive iPSC (c) H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone ... factor, insulator associated protein; ESC, embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of...
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Baculovirus mediated genetic modification of human embryonic stem cells
... properties to combat specific diseases in the course of ex vivo gene therapy 1.1.1 Human Embryonic Stem Cells Human embryonic stem (hES) cells are cells derived from the inner cell mass of human blastocysts ... characterized the integration sites of the transgene expression cassette VII Finally, the effects of the baculoviral transduction of the human embryonic stem cells were investigated The transduction of human ... quantities of high-titer virus stock Although lentivirus vectors are the most successfully and widely used vectors for the genetic modification of human embryonic stem cells, the random nature of their...
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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
... one of the earliest markers of a haematopoietic cell/ progenitor Another haematopoietic progenitor marker is c-Kit which is the receptor for the cytokine Stem Cell Factor (SCF/ Steel Factor) ... with the isolation of embryonic stem cells Extensive investigation of the properties and capabilities of these cells has led to the development of several experimental approaches to induce haematopoietic ... to support formation of typically haematopoietic “cobblestone areas” from progenitor cells of human bone marrow origin (Torok-Storb et al 1999) One of the first reports of hESC-derived haematopoietic...
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IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS
... mandatory 1.5 Human Embryonic stem cells As the name suggests, the human embryonic stem cells are derived from the early embryo These cells have the capacity to self renew and can be directed to ... Although, these cells have a high capacity to self renew in culture, their ability to expand is less than that for embryonic stem cells The self renewal and the proliferative capacity of these stem cells ... Derivation of HES cells Human embryonic stem cells are derived from the inner cell mass of the developing blastocyst- stage embryo The blastocyst is structurally a hollow sphere composed of the outer...
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Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28
... Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells are isolated from the inner cell mass (ICM) of embryos of blastocyst ... indicated the uniqueness of the human ES cells from the rest of the cells, cancerous or normal and that human EC cells shared a common core set of pluripotency genes with human ES cells In the ES/EC ... embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells 1.1.2 Regulatory networks and transcription factors in human ES cells 1.1.3 Induced pluripotent stem cells 1.1.4 Human...
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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... recruitment of factors to the a-globin gene locus during the differentiation of erythroid cells The regulatory elements located upstream of the a-globin genes also appear to initiate the activation of the ... To address the question of whether HS3-specific transcription is unique to the mouse embryonic stem cell system, we also analyzed transcription in the b-globin gene locus in human CD133+ hematopoietic ... ES cells and show that these marks occur independently of the recruitment of any lineage-specific transcription factors such as PU.1 Furthermore, they observe the presence of components of the...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... Figure Effects of the transplanted vascular cells on the CBF in the ipsilateral side Effects of the transplanted vascular cells on the CBF in the ipsilateral side A-C: LDPI analysis of the CBF by ... corresponded to the area under MCA In the groups, the CBF ratio on day decreased by about 20% compared to that of the contralateral side due to ligation of the left CCA after the trans- Page of 14 (page ... for therapeutic vascular regeneration after stroke Abbreviations ES cells: Embryonic stem cells; VEGF-R2: vascular endothelial growth factor receptor type 2; ECs: endothelial cells; MCs: mural cells; ...
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Báo cáo y học: "Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells" pdf
... expression of osteogenic markers, as a result of chordin knockdown in cells from all donors tested Another factor to consider is whether the age of the donors had an influence on the results of this ... during the osteogenic differentiation of the progenitor cells To date, biological methods of enhancing bone regeneration in clinical use have centred around the promotion of osteoinduction via the ... number of cells was determined spectrophotometrically entiation of human mesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... according to standard protocols [21] The isolated λ gt10 DNA was then digested with EcoRI to release the 1.7 kB sized BMP-4 cDNA insert, which was then cloned into the EcoRI site of the pAdlox ... by the proximity of the cells to the joint space and the bone Page 10 of 15 (page number not for citation purposes) respectively, alignment of the chondrocytes along the arcades of Benninghoff ... approximated the size of lacunae noted in the calcifying zone of the human growth plate In contrast, the lacunae that formed in the presence of BMP-4 were closer in size to those of the hypertrophic...
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Báo cáo y học: " Comprehensive transcriptome analysis of mouse embryonic stem cell adipogenesis unravels new processes of adipocyte development" ppt
... development The observation that the mESC culture system might not only allow the generation of adipose stem cells, but also of their potential vascular niche, opens new avenues for the study of adipose ... conception of the bioinformatics studies, and design of the figures JR conceived the g:Profiler web toolkit, carried out PPI analysis, participated in the conception and the design of the bioinformatics ... subset of adipocytes in the cranial region of the body is generated by the neural crest, rather than by mesodermal progenitors, classically thought to be at the origin of this lineage [26] Furthermore,...
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Báo cáo y học: "Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning" ppt
... of the BMP2+ cells to cardiac cells Differentiation of the BMP2+ cells to cardiac cells (a) RT-PCR analysis of the cardiac markers in BMP2+ cells and other controls (b) Schematic outline of the ... Detection of smooth muscle cells after differentiation of the BMP2+ cells Figure Detection of smooth muscle cells after differentiation of the BMP2+ cells (a) Expression of SMA in the BMP2+ cells ... into right and left portions and then migrates to various locations within the embryo to give rise to most structures of the peripheral nervous system, such as Schwann and glia cells of the autonomic...
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Báo cáo y học: "Global transcriptome analysis of murine embryonic stem cell-derived cardiomyocytes" doc
... will be to identify the complete cell specific transcriptome Identification of the complete transcriptome of the ES cell derived somatic cells will contribute to our understanding of the developmental ... and compared with the undifferentiated αMHC embryonic stem cells) 'Count' refers to the number of transcripts in the respective category; the 'P value' column shows the value of Fisher's exact ... profiling remains of importance within the context of defining the cardiomyocyte specific transcriptome Conclusion After the completion of several mammalian genome sequencing projects, the next...
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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
... al 2007;Functional network analysis of the transcriptomes of mesenchymal stem cells derived from amniotic fluid, amniotic membrane, cord blood, and bone marrow Stem Cells (10):2511-2523 106 Rosen ... stromal cells Biology of adult mesenchymal stem cells: regulation of niche, self-renewal and differentiation Arthritis Res Ther (1):204 121 Owen M, Peck W 1985;Lineage of osteogenic cells and their ... deletion from the basal compartment of the mammary epithelium affects stem cells Nat Cell Biol (6):716-722 99 Woodward TL, Mienaltowski AS, Modi RR, et al 2001;Fibronectin and the alpha(5)beta(1)...
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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
... by the breakdown of the joint’s cartilage Cartilage is the part of the joint that cushions the ends of the bones and allows easy movement of joints The breakdown of cartilage causes the bones to ... functionality of ESCs 2.2 Human Embryonic Stem Cells (hESCs) Embryonic stem cells (ESCs) are derived from the inner cell mass of the blastocyst-stage embryos and are characterized by their capacity to be ... 2008) Human embryonic stem cells (hESCs) are stem cell lines of embryonic origin, isolated from the inner cell mass of the blastocyst-stage embryo (Thomson et al., 1998) Human ESCs have the capacity...
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Establishment of autologous culture systems for human embryonic stem cells
... Characterization of stem cells by derivation source Category Embryonic stem cells Embryonic germ cells Fetal stem cells Umbilical cord stem cells Umbilical cord blood stem cells Umbilical cord matrix stem cells ... rise to limited types of cells which are in the closely related families (Sorgner, 2007) Embryonic stem cells Mesenchymal stem cells, Hematopoietic stem cells Oligopotent Can differentiate into ... plasticity, stem cells can also be classified into six broad groups according to their derivation sources, named embryonic stem cells Literature review (ESCs), embryonic germ cells, fetal stem cells, ...
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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives
... Klf4 into mouse embryonic fibroblasts The expression of these factors were sufficient to reprogram the cells back into a pluripotent state The iPSC were shown to be able to differentiate into cells ... 2006) 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have two characteristics that distinguish them from other stem cells, in that they are capable of long-term ... form the dorsal part of the tube while the area closest to the underlying notochord is the ventral area 10 After neurulation, the epithelial cells on the neural plate (or neuroepithelial cells) ...
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