analysis gel electrophoresis and data analysis
Báo cáo khoa học: "Two-dimensional gel electrophoresis and immunoblot analysis of Neospora caninum tachyzoites" ppt
... 80 kDa band, spots 15, 16, 17, 18 and 19 to 65 kDa band, spot 37 to 55 kDa band, spot 44 and 46 to 44 kDa band, spot 52 to 39 kDa band, spot 55 and 56 to 37 kDa band, spot 58 to 35 kDa band, spot ... molecules of 16/17, 29, 37, 46 kDa Two-dimensional gel electrophoresis and its immunoblot analysis of Neospora caninum tachyzoites 143 Fig Analysis of 2-DE and immunoblot profiles of N caninum tachyzoites ... kDa band, spot 63 to 28 kDa band, spot 64 to 15.5 kDa band Spot 42, 54 and 61 exhibited strong signals on 2-DE immunoblotting profile on 144 Eung-Goo Lee et al Fig SDS-PAGE and its immunoblot analysis...
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Báo cáo y học: "Proteomic profiling of the mesenteric lymph after hemorrhagic shock: Differential gel electrophoresis and mass spectrometry analysis" pdf
... labeling and 2D-DIGE analysis KH, AZ and MD performed mass spectrometry data collection and data analysis KH, EP, AZ, EM, MD and AB drafted the manuscript FG carried out the western analysis KH, AB and ... gel analysis: two-dimensional difference gel electrophoresis incorporating a pooled internal standard Proteomics 2003, 3:36-44 28 Tannu NS, Hemby SE: Two-dimensional fluorescence difference gel ... image of the Cy and Cy3 scans from the analytical gel used for additional spot picking and protein identifications The pH and MW range are the same as in Figure 50 μg of pre and post-shock was...
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Báo cáo y học: "Psoriasin, one of several new proteins identified in nasal lavage fluid from allergic and non-allergic individuals using 2-dimensional gel electrophoresis and mass spectrometry" potx
... dimension gels SDS-PAGE was performed at constant effect (10 W /gel) for about h and 30 using the Ettan DALT II system (Amersham Biosciences) Staining of gels and gel image analysis Second dimension gels ... 2-DE, gel image analysis, analysis of MALDI results, database search, western blot and drafted the manuscript MA and LOC conceived the study, participated in its design and coordination and helped ... Pteronyssimus and D Farinae), molds (Cladosporium and Alternaria) and animal allergens (cat, dog and horse) SPT were performed on the volar side of the forearm with saline buffer as negative and histamine...
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Báo cáo khoa học: Analysis of the CK2-dependent phosphorylation of serine 13 in Cdc37 using a phospho-specific antibody and phospho-affinity gel electrophoresis doc
... analyzed by phospho-affinity gel electrophoresis and stained with CBB phospho-affinity gel electrophoresis Phosphorylation was rapid and detected within (Fig 8A, lane 4) and completed within at 30 ... phospho-affinity gel electrophoresis followed by western blotting with anti-Cdc37 Samples were analyzed after dephosphorylation for (lanes and 7), (lanes and 8), 10 (lanes and 9), and 20 (lanes and 10) ... of Mg2+ and ⁄ or ATP as indicated, was incubated at 30 °C for 30 min, analyzed by phospho-affinity gel electrophoresis, and then stained with CBB (B) Wild-type protein (lanes 1, and 7) and two...
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báo cáo khoa học: " Evaluation of protein pattern changes in roots and leaves of Zea mays plants in response to nitrate availability by two-dimensional gel electrophoresis analysis" pdf
... sugars and sucrose in roots (A, C, E and G) and leaves (B, D, F, and H) and chlorophyll content in leaves (I) of Zea mays plants, previously grown in the absence of nitrogen for 17 days (T0) and ... with spray voltage and capillary temperature set at kV and at 220°C, respectively Data were collected in the full-scan and data dependent MS/MS mode with collision energy of 35% and a dynamic exclusion ... proteomic techniques, an appropriate experimental design, and data analysis to fulfil MIAPE standards, increase plant proteome coverage and expand biological knowledge J Proteomics 2009, 72:285-314...
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Tài liệu Fundamental Numerical Methods and Data Analysis doc
... by the "right hand rule" while the magnitude depends on the rate of change of the x- and ycomponents of the vector field with respect to y and x 19 Numerical Methods and Data Analysis The curl ... is 27 Numerical Methods and Data Analysis determined by the analyst and he must be careful not to aim too high and carry out grossly inefficient calculations, or too low and obtain meaningless ... eliminated concerns of random error, but round off error can be a persistent problem Numerical Methods and Data Analysis The extreme speed of contemporary machines has tremendously expanded the scope...
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Tài liệu An Introduction to Statistical Inference and Data Analysis docx
... from them The union of A and B is the set A ∪ B = {x ∈ S : x ∈ A or x ∈ B} and the intersection of A and B is the set A ∩ B = {x ∈ S : x ∈ A and x ∈ B} Notice that unions and intersections are symmetric ... left hand and another on your right hand How many ways can this be done? First, suppose that you wear the diamond ring on your left hand Then there are three rings available for your right hand: ... such hands Hence, P (A) = #(A) 37 = 40 = = 0004 #(S) 2470 (c) One hand of four cards is dealt to Arlen and a second hand of four cards is dealt to Mike What is the probability that Arlen’s hand...
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STATISTICS AND DATA ANALYSIS FOR THE BEHAVIORAl SCIENCES docx
... Overview of Power Analysis: Thinking More Critically About Research and Data Analysis 400 ,) Knowledge Base 402 Planning for Data Analysis: Developing a Before and After Data Collection Analysis Plan ... Interquartile and the Semi-Interquartile Range 153 Variance and Standard Deviation 155 Sample Variance and Standard Deviation 157 Homogeneity and Heterogeneity: Understanding the Standard Deviations ... Calcuklting Variance and Standard Deviation from a Data Array 160 Population Variance and Standard Deviation 161 Looking Ahead: Biased and Unbiased Estimators of Variance and Standard Deviation 162...
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problem solving and data analysis using minitab
... Term and Overall Capability 8.3 Capability Analysis for Normal Data 8.4 Capability Analysis for Non Normal Data 8.5 Capability Comparison using the Assistant 313 313 318 319 329 340 Correlation and ... hear and I forget I see and I remember I and I understand’ This proverb sums up the spirit of this book as it is very practical and it involves the reader Every chapter contains examples and exercises ... endpoint of the analysis The example and exercise data sets can be downloaded from the Wiley publishing website, www.wiley.com/go/six_sigma_methodology Problem Solving and Data Analysis using...
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jean - luc starck, fionn murtagh - astronomical image and data analysis
... if the data presents highly anisotropic features, the ridgelet transform (Cand`s and Donoho, 1999; Cand`s and Donoho, 1999) e e or the curvelet transform (Donoho and Duncan, 2000; Cand`s and Donoho, ... processing and pattern recognition is available on-line (Campbell and Murtagh, 2001) Data analysis texts of importance include Bishop (1995), and Ripley (1995) 1.2 Transformation and Data Representation ... methods, and by dimensionality mapping methods Multiband images can be taken as a particular case Such methods are pivotal in Chapter on multichannel data, on catalog analysis, and on data storage and...
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john a rice mathematical statistics and data analysis, second edition 1994
... one on theory and one on data analysis, seems to me artificial Furthermore, many students take only one course in statistics and not have time for two or more Analysis of Data and the Practice ... equally likely, and P(A) is not EXAMPLE B A black urn contains red and green balls and a white urn contains red and green balls You are allowed to choose an urn and then choose a ball at random from ... Congress Cataloging-in-Publication Data Rice, John A., Mathematical statistics and data analysis / John A Rice —2nd ed p cm Includes bibliographical references and indexes ISBN 0-534-20934-3 (acid-free)...
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Báo cáo khoa hoc:" Simulation analysis to test the influence of model adequacy and data structure on the estimation of genetic parameters for traits with direct and maternal effects" ppsx
... 3.2 Data analysis Data were analysed using the model with direct and maternal genetic effects and environmental maternal effects (model AMRC) A fixed flock effect was fitted in all situations The data ... (o) and Wi (m) are Mendelian deviations of the offspring (i) for the direct effect (o) and the maternal effect (m), respectively; R i and Ri are independent random variables sampled from a standard ... above for populations and The average and the empirical standard deviation were calculated over the fifty replicates obtained for each model and each population 2.3 Results and discussion Results...
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Báo cáo y học: "ptimized design and data analysis of tag-based cytosine methylation assays" pps
... contributions MS and JMG designed the assays and strategies for its analysis, MS performed all library preparation and characterization, MS, DL and MP performed bisulfite validation studies, while QJ and ... strengths of MSCC and HELP-seq/Methylseq, and the supporting analytical workflow that maximizes the quantitative capabilities of the data generated Results and discussion Library preparation and sequencing ... workflow in Figure and an example of a UCSC genome browser representation of HELP-tagging data in Figure S1 in Additional file All data are available through the Gene Expression Omnibus database (accession...
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A Practical Introduction to Structure, Mechanism, and Data Analysis - Part 2 pptx
... Chapters 4, 6, and 8, the reversible formation of binary complexes between enzymes and ligand molecules (i.e., substrates and inhibitors) is a critical aspect of both enzymatic catalysis and enzyme ... peptide carbon and the oxygen, and single-bond character between the peptide carbon and nitrogen atoms Table 3.2 provides typical bond lengths for carbon—oxygen and carbon—nitrogen double and single ... protein structure (Chapter 3) and enzyme—ligand binding (Chapters 4, 6, and 8); these are electrostatic interactions, hydrogen bonding, hydrophobic interactions, and van der Waals forces Here...
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A Practical Introduction to Structure, Mechanism, and Data Analysis - Part 3 pot
... results from a ligand binding experiment; the data show the effects of nonspecific ligand binding on the titration data Triangles illustrate the typical experimental data for total ligand binding, ... make visual inspection of the binding data much easier and are highly recommended for presentation of such data GRAPHIC ANALYSIS OF EQUILIBRIUM LIGAND BINDING DATA 89 Semilog plots allow for rapid ... standard equation for a straight line, we have: y : (mx) ; b (4.31) GRAPHIC ANALYSIS OF EQUILIBRIUM LIGAND BINDING DATA 91 Figure 4.9 Double-reciprocal plot for the data in Figure 4.3 The data...
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A Practical Introduction to Structure, Mechanism, and Data Analysis - Part 4 ppsx
... at latter time results from free ligand depletion from the column due to the binding events [Data redrawn from Hummel and Dreyer (1962).] bound and free ligand concentrations Hence, during protein ... Fless, G., Scanu, A., and Copeland, R A (1993) J Protein Chem 12, 39 Bell, J E., and Bell, E T (1988) Proteins and Enzymes, Prentice-Hall, Englewood Cliffs, NJ Campbell, I D., and Dwek, R A (1984) ... Haldane and K G Stern, Eds., Steinkopf, Dresden, pp 119ff Zeeberg, B., and Caplow, M (1979) Biochemistry, 18, 3880 Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis...
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A Practical Introduction to Structure, Mechanism, and Data Analysis - Part 5 docx
... these powerful techniques REFERENCES AND FURTHER READING Bell, J E., and Bell, E T (1988) Proteins and Enzymes, Prentice-Hall, Englewood Cliffs, NJ Briggs, G E., and Haldane, J B S (1925) Biochem ... T.-Y., Stein, R L., and Hagmann, W K (1993) J Med Chem 36, 4293 Cleland, W W (1967) Adv Enzymol 29 1—65 Copeland, R A (1991) Proc Natl Acad Sci USA 88, 7281 Cornish-Bowden, A., and Wharton, C W ... Biochemistry, 22, 3581 Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis Robert A Copeland Copyright 2000 by Wiley-VCH, Inc ISBNs: 0-471-35929-7 (Hardback); 0-471-22063-9...
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A Practical Introduction to Structure, Mechanism, and Data Analysis - Part 6 pot
... intensity of the quinine sulfate standard at 453 nm, when the same fluorometer is used to excite both sample and standard, at the same wavelength Of course, both sample and standard measurements must be ... Biochem J 308, 305 Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis Robert A Copeland Copyright 2000 by Wiley-VCH, Inc ISBNs: 0-471-35929-7 (Hardback); 0-471-22063-9 ... of enzyme catalysis and on the interactions of enzymes with ligands, such as substrates and inhibitors Chapter provided the basis for determining the kinetic constants k and K from initial velocity...
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A Practical Introduction to Structure, Mechanism, and Data Analysis - Part 7 pdf
... — contaminating protein bands may obscure the analysis of the substrate and product bands on the gel A common strategy in these cases is to perform Western blotting analysis using an antibody ... temperature for 1—5 minutes and loaded onto a gel of an appropriate percentage acrylamide to effect separation Current is applied to the gel from a power source, and the electrophoresis is allowed ... the gel (For a 10% gel, a typical electrophoretic run would be performed at 120 V constant voltage for 1.5—3 h, depending on the size of the gel) After electrophoresis, protein or peptide bands...
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