an expression of function
Báo cáo khoa hoc:" An expression of mixed animal model means equations to account for different and variances in the base" pps
... effects; a is the random b vector of breeding values of base animals; a is the random vector of breeding r values of non-base animals; e is the random vector of residuals; and X, Z and l b , r Z are ... H6 van = , b HQ = b H Hb CONCLUSION When additive genetic means and variances of base animals are not homoprediction of breeding values can be obtained by means of animal models if the covariance ... a and a respectively The vector of breeding values of non-base animals can be partitioned as: * where s is a linear transformation of the random vector of the Mendelian sampling effects (s) of...
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Tài liệu Báo cáo khoa học: Expression and function of Noxo1c, an alternative splicing form of the NADPH oxidase organizer 1 doc
... al Expression and function of Noxo1c A B C Fig Alternative splice forms of human Noxo1 (A) The genomic organization of human NOXO1 gene Translated sequences are shown as black boxes, and untranslated ... 3665 Expression and function of Noxo1c R Takeya et al Expression of Noxo1b and Noxo1c in various human tissues To compare the distribution pattern of Noxo1b and Noxo1c, we designed variant-specific ... compilation ª 2006 FEBS 3673 Expression and function of Noxo1c R Takeya et al Expression of Noxo1b and Noxo1c in various human tissues The expression patterns of the Noxo1b and Noxo1c messengers were...
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Báo cáo khoa học: Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin pptx
... cleavage of fusion protein and purification of the recombinant BmKIM The fusion protein was cleaved completely with an enterokinase/substrate ratio of 50% at 26 °C for 10 on the Fig Expression and ... possess different affinity and induce different functions provides valuable information for the study of nerve and cardiac Na+ channels No doubt, functional expression of BmKIM would make it possible ... molecular basis for the wide toxic range of BmKIM ACKNOWLEDGEMENTS We thank Wang Teng and Dr Wang Xi for providing the coordinates for the electrophysiology of BmKIM; Prof Ma Hui-wen for his kindness...
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Intelligent Software Agents on the Internet: an inventory of currently offered functionality in the information society & a prediction of (near-)future developments
... types handle Computer agents (in the short and medium term) will handle standard tasks and all those tasks that a computer program (i.e an agent) can faster or better than a human can Human intermediaries ... example will be given to give an impression of the new forms of functionality it can offer 4.3.1 Middle layer (agent) functions "The fall in the cost of gathering and transmitting information will ... levels and patterns of exchanges Their ability to handle complex, albeit mechanical, transactions, to process millions of bits of information per second, and to act in a demonstrably evenhanded...
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an inventory of currently offered functionality in the information society & a prediction of (near-)future developments
... types handle Computer agents (in the short and medium term) will handle standard tasks and all those tasks that a computer program (i.e an agent) can faster or better than a human can Human intermediaries ... example will be given to give an impression of the new forms of functionality it can offer 4.3.1 Middle layer (agent) functions "The fall in the cost of gathering and transmitting information will ... levels and patterns of exchanges Their ability to handle complex, albeit mechanical, transactions, to process millions of bits of information per second, and to act in a demonstrably evenhanded...
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Tài liệu Báo cáo khoa học: Differential expression of duplicated LDH-A genes during temperature acclimation of weatherfish Misgurnus fossilis Functional consequences for the enzyme ppt
... total activity) of an enzyme can be changed by affecting rates of transcription and ⁄ or translation and ⁄ or mRNA and protein degradation This represents a quantitative strategy to offset the lack ... Germany) cDNA sequences of both isoforms of LDH-A mRNA can be obtained from GenBank under following accession numbers: DQ991254 for LDH-AS and DQ991253 for LDH-AL Quantification of LDH-A transcripts ... understanding of the functional and metabolic consequences, further study needs to identify the kinetic, thermodynamic and regulatory properties of recombinant LDH-Aa4 and LDH-Ab4 homotetramers and...
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Tài liệu Báo cáo khoa học: Analysis of oxidative events induced by expanded polyglutamine huntingtin exon 1 that are differentially restored by expression of heat shock proteins or treatment with an antioxidant ppt
... insoluble fibers in affected neurons [8] In human and HD transgenic mice, the disease correlates with the appearance of intraneuronal, intranuclear and perinuclear aggregates ⁄ inclusions containing ... [39] and are associated with aggregates in the brains of HD transgenic mice [40] Hsp70 and Hdj-1 can inhibit polyQ aggregation and reduce the size of htt-polyQ inclusion bodies [15,36,37] and ... molecular mass range) that already display a basal level of oxidation in control cells Expression of Hsp70 ⁄ Hdj-1 or Hsp27 did not significantly change the pattern and level of oxidized proteins...
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Tài liệu Báo cáo khoa học: Expression of an a-1,3-glucanase during mycoparasitic interaction of Trichoderma asperellum docx
... (a-1,3- and a-1,6-glucan), nigeran (a-1,3- and a-1,4-glucan), soluble starch (a-1,4- and a-1,6-glucan), pustulan (b-1,6-glucan), laminarin (b-1,3-glucan), carboxymethilcelullose (b-1,4-glucan) or ... in other mutanases (WNDYGES) AGN13.2 presented an overall protein sequence identity of 79 and 77% to the previously cloned a-1,3-glucanases in T harzianum CECT2413 [25] and T harzianum CBS243.71 ... interaction of T harzianum and B cinerea and T harzianum with itself Table Biochemical characteristics of a-1,3-glucanases in Trichoderma sp Origin T asperellum CECT20539 T harzianum CECT2413...
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Tài liệu Báo cáo khoa học: Characterization and functional expression of cDNAs encoding thyrotropin-releasing hormone receptor from Xenopus laevis Identification of a novel subtype of thyrotropin-releasing hormone receptor ppt
... brain (lane 4), testis (lane 5), urinary bladder (lane 6), stomach (lane 7), lung (lane 8), heart (lane 9), intestine (lane 10) and dorsal skin (lane 11) Rat testis (lane 1) and ovary (lane 2) ... current consists of a large, rapid and transient response that is typical of Ca2+-dependent Cl– channels activated after stimulation of PLC and the subsequent InsP3-dependent mobilization of Ca2+ from ... active than TRHR1, acting via pathways mediated by the transcription factors AP-1, Elk1 and CREB [35] To clarify the functional significance of the TRH ligand/ receptor system in amphibians, a species...
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Báo cáo khoa học: Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening docx
... Identification of ligands for olfactory receptors by functional expression of a receptor library Cell 95, 917–926 20 Hatt H, Lang K & Gisselmann G (2001) Functional expression and characterization of odorant ... believe that our luciferase sensor can be at the basis of efficient, rapid and low cost screening of a large range of odorants The signal can be significantly enhanced through Ga subunit engineering ... yeast and mammalian GPCR signaling pathways, this assay enables the quantitative measurement of receptor activity, or alternately the detection of its ligands Using known ligands of I7 OR (heptanal,...
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Báo cáo khoa học: Sulfation of hydroxychlorobiphenyls Molecular cloning, expression, and functional characterization of zebrafish SULT1 sulfotransferases docx
... Detection of zebrafish SULT1 ST1 and ST2 mRNAs and (B) Western blot analysis of zebrafish SULT1 ST1 protein (A) Detection of zebrafish SULT1 ST1 and ST2 mRNAs in cultured zebrafish cells (lanes and 3) and ... xenoestrogen-sulfating activities of the human cytosolic sulfotransferases: molecular cloning, expression, and purification of human SULT2B1a and SULT2B1b sulfotransferases Biochim Biophys Acta ... activation and transfer Science 128, 575–580 Coughtrie, M.W.H., Sharp, S., Maxwell, K & Innes, N.P (1998) Biology and function of the reversible sulfation pathway catalysed by human sulfotransferases and...
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Báo cáo khoa học: Identification and functional expression of a second human b-galactoside a2,6-sialyltransferase, ST6Gal II docx
... The results of Northern and expression array analyses clearly indicated a restricted and low level expression of hST6Gal II gene as an kb transcript mainly in brain, which is in accordance with ... dialyzed and lyophilized prior to use The carbohydrate content of asialo-glycoproteins was analysed by GC-MS [30] Construction of expression vectors of hST6Gal II and transfections A truncated form of ... human ST6Gal II (Eur J Biochem 270) 953 Fig Nucleotide and predicted amino acid sequence (A) and hydropathy profile (B) of human ST6Gal II, and (C) comparison of the sialylmotifs L, S and VS of...
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Báo cáo Y học: Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides ppt
... a-D-galactopyranoside, a-L-arabinopyranoside, b-L-arabinopyranoside, a-L-arabino-furanoside, a-D-mannopyranoside, a-D-mannopyranoside, a-D-fucopyranoside, a-D-xylopyranoside, a-L-rhamnopyranoside) was determined ... pastoris and the transformants screened for the best expression performances Muts transformants were grown under noninduced conditions (MGY or BMGY) and then transferred to medium containing methanol ... release of NP using 4NP-a-D-glucopyranoside, 4NP-a-Larabinofuranoside, 4NP-a-L-rhamnopyranoside, 4NP-a-Dmannopyranoside or 4NP-b-D-mannopyrano-side The activity towards 2NP-galactopyranoside...
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Báo cáo khoa học: Molecular cloning and functional expression of the human sodium channel b1B subunit, a novel splicing variant of the b1 subunit potx
... that the human b1B subunit may be another candidate useful for studying the mechanism of upregulation of functional sodium channels on the cell surface and increasing the rate of spontaneous firing ... blot analysis of the gene expression of human b1B (A,B) and b1 (C,D) subunits, using human b-actin mRNA level as the control (E,F) (A), (C) and (E) are human multiple tissue blots; (B), (D) and ... Sequence analysis and genomic structure of the human sodium channel b1B subunit (A) Amino acid sequence comparisons between human b1B and b1 subunits The signal peptide sequence and transmembrane...
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Báo cáo khoa học: Functional expression of the quinoline 2-oxidoreductase genes (qorMSL) in Pseudomonas putida KT2440 pUF1 and in P. putida 86-1 Dqor pUF1 and analysis of the Qor proteins doc
... kanamycin resistant mutants still formed Qor PCR analyses confirmed that nptII was replacing one copy of qorMSL and that P putida 86-1 contains more than one copy of the qor genes and their flanking ... estimated from Hanes plots Determination of the metal contents of the Qor proteins, and detection of the nucleotide moiety of the molybdenum cofactor The contents of molybdenum and iron were determined ... digested with XhoI and DraIII to remove nptII After the removal of the 3¢ overhang and the filling of the 5¢ overhang of the plasmid with T4 DNApolymerase, a PCR amplificate of aacC1 [46] was inserted...
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Báo cáo khoa học: Functional expression of Pseudomonas aeruginosa GDP-4-keto6-deoxy-D-mannose reductase which synthesizes GDP-rhamnose docx
... structural analysis with MALDI-TOF MS and NMR Protein expression and analysis The GAL1 and GAL10 promoters of the pESC-LEU expression vector are repressed by dextrose and induced by galactose In the expression ... of the lack of a standard for this nucleotide sugar Fig Western blots of the expression of GMD and RMD in S cerevisiae YPH501 detected with c-Myc antibody (A) and FLAG antibody (B) Lane 1, YPH501(pESC-LEU), ... control; lane 2, YPH501(pHP1); lane 3, YPH501(pRHA1); lane 4, YPH501(pRHA2) The presence of 44-kDa H pylori GMD was detected in lanes and 4, and the presence of 34-kDa P aeruginosa RMD in lanes and...
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Báo cáo Y học: The expression of glutathione reductase in the male reproductive system of rats supports the enzymatic basis of glutathione function in spermatogenesis doc
... contribution of spontaneous NADPH oxidation and other reductases in the samples can be ignored One unit of GR activity was defined as the amount of enzyme that catalyzes the oxidation of lmol of NADPH ... absorbance change using authentic GSH and Ó FEBS 2002 1572 T Kaneko et al (Eur J Biochem 269) GSSG as a standard and the data are expressed as nmolÆmg protein)1 SDS/PAGE and Western blot analysis ... [28] and then transferred onto Hybond-P (Amersham Pharmacia, Pscataway, NJ, USA) under semi-dry conditions by means of a Transfer-blot SD Semi-dry transfer cell (Bio-Rad, Tokyo, Japan) The membranes...
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ACCOUNTING SERVICES: AN OVERVIEW OF THE ACCOUNTING FUNCTIONS AT WESTERN MICHIGAN UNIVERSITY pdf
... quarterly, and annual financial reports Responsible for the financial reporting systems, PeopleSoft and GLOW, and the on line journal entry system (JES) for the University Assure compliance for ... tangible or intangible Cash, accounts receivable, land What the university owes Accounts payable Long-term debt An example of an asset and a liability for most of us is our home and mortgage The ... departments, balance gets swept clean at beginning of each fiscal year Scholarships, Designated, Student loan Annuity and Life Income, some Auxiliary, Agency and Trust, Foundations, Insurance &...
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Báo cáo khoa học: Recombinant expression of an insulin-like peptide 3 (INSL3) precursor and its enzymatic conversion to mature human INSL3 pot
... Recombinant expression of an INSL3 precursor A B C Fig (A) Amino acid sequence and nucleotide sequence of the recombinant human INSL3 precursor The B-chain and A-chain are shown in red and green, ... B D C Fig Expression and purification of the human INSL3 precursor (A) Analysis of the expression of INSL3 precursor by tricine SDS ⁄ PAGE Fifty microlitres of culture broth before and after IPTG ... spectra of the human INSL3 precursor before and after in vitro refolding (B) Far-UV spectra of the mature human INSL3 and porcine insulin Functional cAMP assay The activity of the mature INSL3 and...
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Báo cáo khoa học: Expression and functional characterization of P2Y1 and P2Y12 nucleotide receptors in long-term serum-deprived glioma C6 cells ppt
... proliferative and differentiation state, basal level of ERK1 ⁄ and Akt kinase phosphorylation, and P2Y1 and P2Y12 receptor protein expression and functional activity Examination of the effects of specific ... different expression of P2Y1 and P2Y12 receptors in serum-starved and nonstarved cells seems to have an important biological significance Given the shift in receptor expression, there is simultaneous ... h of serum starvation resulted in its increase, showing that exhibition of a high basal level of Akt activity is part of an important self-regulatory mechanism This process was only stopped, and...
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