amp mouse obese gene promoters 200 bp upstream from tss
Báo cáo Y học: The proximal cis-acting elements Sp1, Sp3 and E2F regulate mouse mer gene transcription in Sertoli cells potx
Ngày tải lên :
08/03/2014, 23:20
... RACE products amplified using transcripts isolated from mouse testis and Mer Ó FEBS 2002 Mer gene transcription in Sertoli cells (Eur J Biochem 269) 3793 Fig Sequence analysis of mouse Mer 5¢-flanking ... initiated transcription from several start sites including two that were located at +29 and +102 bp upstream from the translation initiation codon (Fig 2B) As the +102 bp position resulted a stronger ... 5¢-GCCAAACTTCTTG GATTCGCAGCG-3¢ (S16, antisense), which generated a 384 -bp S16 product Total RNA (25 lg per sample, except for Mer where 60 lg was used), isolated from testes of BALB/c mice using Trizol as...
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báo cáo khoa học: " Seed storage protein gene promoters contain conserved DNA motifs in Brassicaceae, Fabaceae and Poaceae" ppt
Ngày tải lên :
12/08/2014, 03:21
... signatures of SSP gene promoters in the different species Results Seed storage protein gene promoters contain conserved motifs Seed storage protein gene promoter sequences (the 500 bp upstream region ... Type Gene ID F1 Figure PWM score and rank of Fabaceae SSP gene promoter motifs in 14 soybean SSP gene promoters PWM score and rank of Fabaceae SSP gene promoter motifs in 14 soybean SSP gene promoters ... http://www.biomedcentral.com/1471-2229/9/126 a) Brassicaceae -500 -400 -300 -200 -100 TSS -400 -300 -200 -100 TSS -400 -300 -200 -100 BT, F3, P4 B1, B3, F1, F2 B2, F5 F4 P1 P2, P3 P5 P6 P7 TSS b) Fabaceae -500 c) Poaceae -500 Figure...
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Báo cáo Y học: Cloning, expression and characterization of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces ansochromogenes pot
Ngày tải lên :
31/03/2014, 08:20
... SA, NaoA from S ansochromogenes; SC, putative 2-nitropropane dioxygenase from S coelicolor; WS, 2-nitropropane dioxygenase from W saturmus var mrakii; NC, 2-nitropropane dioxygenase from N crassa; ... of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces; partial purification of the related enzyme from Streptomyces has been reported [16] The deduced aminoacid sequence of NaoA from ... flavoprotein nitroalkane oxidase from F oxysporum, whereas these two amino acids were not conserved in NaoA from S ansochromogenes and the putative oxidoreductase from S coelicolor Possibly, NaoA...
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Báo cáo khoa học: "Genetic variability of the prion protein gene (PRNP) in wild ruminants from Italy and Scotland" ppsx
Ngày tải lên :
07/08/2014, 23:22
... open reading frame (ORF) of the PRNP gene (771 bp) were amplified by performing PCR The primers used for the amplification and sequencing of the deer samples from Scotland were 19fwd (5’ ATT TTG ... capreolus) A total of 323 red deer samples were collected from Scotland (n = 132; 82 from the mainland and 50 from the Isle of Rhum) and during the hunting seasons from Italy (n = 191) The chamois ... and roe deer (n = 189) all came from Italy and the sampling covered the whole Alpine arc Genomic DNA was isolated from blood or frozen muscle tissue with the GenElute Mammalian DNA Kit (SigmaAldrich,...
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Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt
Ngày tải lên :
19/02/2014, 06:20
... primers DN+385 and DN+721 used in 3¢-RACE 877 bp D 841 bp E 806 bp F 755 bp G 730 bp H 929 bp I 789 bp J 764 bp 3097 Promoters of human deoxyribonuclease I gene Y Kominato et al that the sequence between ... DN+721 DN-9 DN+89 DN+232 DN+254 DN+785 A 881 bp C FEBS Journal 273 (2006 ) 3094–3105 ª 2006 The Authors Journal compilation ª 2006 FEBS 895 bp B RT-PCR amplified fragments Fig RT-PCR analysis to ... in FEBS Journal 273 (2006 ) 3094–3105 ª 2006 The Authors Journal compilation ª 2006 FEBS 3101 Promoters of human deoxyribonuclease I gene Y Kominato et al the human DNASE1 gene was performed with...
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Tài liệu Báo cáo khoa học: Multiple promoters regulate tissue-specific alternative splicing of the human kallikrein gene, KLK11⁄hippostasin ppt
Ngày tải lên :
19/02/2014, 06:20
... Journal 273 (2006 ) 3678–3686 ª 2006 The Authors Journal compilation ª 2006 FEBS 3679 Multiple promoters of the human KLK11 gene 3680 S Mitsui et al FEBS Journal 273 (2006 ) 3678–3686 ª 2006 The Authors ... kallikreins FEBS Journal 273 (2006 ) 3678–3686 ª 2006 The Authors Journal compilation ª 2006 FEBS 3683 Multiple promoters of the human KLK11 gene S Mitsui et al Whereas KLK11 gene expression is regulated ... Journal 273 (2006 ) 3678–3686 ª 2006 The Authors Journal compilation ª 2006 FEBS 3685 Multiple promoters of the human KLK11 gene S Mitsui et al References Borgono CA & Diamandis EP (2004 ) The emerging...
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Tài liệu Báo cáo khoa học: Characterization of the promoter for the mouse a3 integrin gene Involvement of the Ets-family of transcription factors in the promoter activity doc
Ngày tải lên :
21/02/2014, 03:20
... expression analysis of the 5¢-flanking region of mouse integrin a3 subunit gene We previously cloned the 5¢-flanking region of the integrin a3 subunit gene from a mouse genomic library [18] The restriction ... ectodermal ridge formation and organogenesis in the mouse Development 126, 3957–3968 50 Sementchenko, V.I & Watson, D.K (2000 ) Ets target genes: past, present and future Oncogene 19, 6533–6548 51 Kaya, ... of exon of the mouse integrin a3 subunit gene We prepared a chimeric construct (pGL-ES), in which the 4.0 kb EcoRI/SacI fragment upstream of exon was inserted into the luciferase gene- containing...
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Báo cáo khoa học: Molecular characterization and gene disruption of mouse lysosomal putative serine carboxypeptidase 1 ppt
Ngày tải lên :
07/03/2014, 03:20
... digestion using a 600 bp PCR-generated probe upstream of exon or by multiplex-PCR, resulting in a 390 bp fragment for the wild-type allele and a 200 bp fragment for the gene trap allele Primer ... SCP (GI:35181448); mouse Ctsa (GI:84042523); mouse Scpep1 (GI:13436038); mouse vitellogenic-like carboxypeptidase VLCP (GI:187952735) systems [11] A recent gene target Ctsa mouse model (CtsaS190A), ... Lubke T ¨ (2006 ) Molecular characterization of the hypothetical 66.3-kDa protein in mouse: lysosomal targeting, FEBS Journal 276 (2009 ) 1356–1369 ª 2009 The Authors Journal compilation ª 2009 FEBS...
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Báo cáo khoa học: Sequences downstream of the transcription initiation site are important for proper initiation and regulation of mouse ribonucleotide reductase R2 gene transcription ppt
Ngày tải lên :
08/03/2014, 02:20
... analysis of the mouse R1 and R2 promoter sequences show that they differ, not only from each other, but also from the common promoter structure found in the majority of eukaryotic genes The mouse R1 ... created from pML(CAT)19 [17] by PCR using one primer complementary to the polylinker upstream from the AdML promoter and a second primer complementary to nucleotide 311–292 bp downstream from the ... TATA-box and TBP binding to the R2 promoter We next studied the importance of TBP and the R2 TATA-box in an in vitro transcription system reconstituted from recombinant mouse TBP, TFIIB, TFIIE,...
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Báo cáo khoa học: The mouse Muc5b mucin gene is transcriptionally regulated by thyroid transcription factor-1 (TTF-1) and GATA-6 transcription factors pdf
Ngày tải lên :
15/03/2014, 00:20
... processes From our data and others, the regulation of mucin genes by GATA factors seems to be more general, and may affect the expression of other mucin genes, such as MUC2, MUC3 and MUC4, as their promoters ... Porchet N (2000 ) From normal respiratory mucosa to epidermoid carcinoma: expression of human mucin genes Int J Cancer 86, 162–168 11 Escande F, Porchet N, Aubert JP & Buisine MP (2002 ) The mouse Muc5b ... Regulation of Muc5b mucin gene by TTF-1 and GATA factors RT-PCR Nuclear extract preparation Total RNAs from cultured cells were prepared using the QIAamp RNA blood mini-kit from Qiagen Total RNA...
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Báo cáo khoa học: Malonyl-CoA decarboxylase (MCD) is differentially regulated in subcellular compartments by 5¢AMP-activated protein kinase (AMPK) Studies using H9c2 cells overexpressing MCD and AMPK by adenoviral gene transfer technique potx
Ngày tải lên :
16/03/2014, 16:20
... Ad.GFP or 25 moi per cell of Ad.CA-AMPK Ad.CA-AMPK (25 moiÆcell)1) were determined to yield optimum CA-AMPKa1(312) expression and activity from series of Ad.CA-AMPK concentrations (5, 10, 25 and ... monoclonal mouse anti-cytochrome c, BD Biosciences Pharmingen, San Diego, CA, USA; monoclonal mouse anti-core subunit, Molecular Probes, Eugene, OR, USA) AMPK assay Both endogeous AMPK and overexpressed ... microlitres of the above sample was then incubated with the synthetic 200 lM AMARA (AMARAASAAALARRR) peptide, 200 lM [32P]ATP[c-P], 0.8 mM dithiothreitol, mM MgCl2, 200 lM AMP in buffer (pH 7.0)...
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Báo cáo khoa học: 17b-Hydroxysteroid dehydrogenase type 11 is a major peroxisome proliferator-activated receptor a-regulated gene in mouse intestine pdf
Ngày tải lên :
16/03/2014, 18:20
... peptides derived from P80 were consistent with those calculated from the peptide sequences from 17b-HSD-4 (gi|1706397|sp.|P51660|) The peptides from P80 also contained 32 peptides from Ezrin (gi|32363497|sp.|P26040|) ... peptides derived from P32 were consistent with those calculated from the peptide sequences from 17b-HSD-11 (gi|16716597| ref|NP_444492.1|), and the masses of 12 peptides matched those from annexin ... shown enlarged (B) Ó FEBS 2004 17b-HSD is a major PPAR a-regulated gene in intestine (Eur J Biochem 271) 4143 more in the intestine than in the liver and isolated from other major protein bands...
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Báo cáo Y học: Induction of chicken ovalbumin upstream promoter-transcription factor I (COUP-TFI ) gene expression is mediated by ETS factor binding sites doc
Ngày tải lên :
17/03/2014, 17:20
... coordinate ETS gene expression Cell 95, 393±407 Fromm, L & Burden, S.J (1998) Synapse-speci®c and neuregulininduced transcription require an ets site that binds GABPalpha/ GABPbeta Genes Dev 12, ... of mouse and human COUP-TF I and II genes Genomics 29, 240±246 Maroulakou, I.G., Papas, T.S & Green, J.E (1994) Dierential expression of ets-1 and ets-2 proto-oncogenes during murine embryogenesis ... colocalize with COUP-TFI on the developing mouse embryo We performed in situ hybridization studies on mouse embryos with COUP-TFI and different ETS factors Mouse embryos 14.5-days-old-were chosen...
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Báo cáo khoa học: Recruitment of coregulator complexes to the b-globin gene locus by TFII-I and upstream stimulatory factor doc
Ngày tải lên :
23/03/2014, 07:20
... and located approximately 15–27 kbp upstream of the embryonic e-globin gene The murine b-globin gene locus, which is depicted on the bottom, consists of four genes which are expressed either ... trimethylated H3K27 at the globin gene locus in K562 cells, consistent with studies from the Blobel labora- FEBS Journal 274 (2007 ) 6065–6073 ª 2007 The Authors Journal compilation ª 2007 FEBS V J Crusselle-Davis ... ec-globin gene promoter (Fig 1B) In K562 cells, CBP and p300 interact efficiently with HS2 but not with the repressed b-globin gene promoter (Fig 1C) There is some interaction between CBP and the...
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Báo cáo khoa học: Differential gene expression in periportal and perivenous mouse hepatocytes potx
Ngày tải lên :
23/03/2014, 10:20
... Journal 273 (2006 ) 5051–5061 ª 2006 The Authors Journal compilation ª 2006 FEBS A Braeuning et al Zonal gene expression in mouse liver Table Zonated genes involved in xenobiotic metabolism If genes ... sulfotransferases Other genes involved in xenobiotic metabolism, FEBS Journal 273 (2006 ) 5051–5061 ª 2006 The Authors Journal compilation ª 2006 FEBS 5055 Zonal gene expression in mouse liver A Braeuning ... the plot Genes with preferential perivenous expression FEBS Journal 273 (2006 ) 5051–5061 ª 2006 The Authors Journal compilation ª 2006 FEBS A Braeuning et al Zonal gene expression in mouse liver...
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Báo cáo khoa học: Hepatocyte-specific interplay of transcription factors at the far-upstream enhancer of the carbamoylphosphate synthetase gene upon glucocorticoid induction doc
Ngày tải lên :
23/03/2014, 10:20
... FEBS Journal 274 (2007 ) 37–45 ª 2006 The Authors Journal compilation ª 2006 FEBS M Hoogenkamp et al Transcription factors at the CPS GRU A C/EBP probe B CPS 160 kDa C/EBPα C/EBPβ no ne FoxA1 FoxA2 ... Journal 274 (2007 ) 37–45 ª 2006 The Authors Journal compilation ª 2006 FEBS M Hoogenkamp et al Transcription factors at the CPS GRU upper strand FTO-2B - 5’ WT- + - + 314 333 C/EBP 345 FoxA 365 ... FEBS Journal 274 (2007 ) 37–45 ª 2006 The Authors Journal compilation ª 2006 FEBS 41 Transcription factors at the CPS GRU + dex/cAMP - FTO-2B - + Rat-1 5’ + FTO-2B - + 3’ 405 C/EBP 333 FTO-2B +dex...
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Báo cáo khoa học: Nuclear factor kappa B and tumor necrosis factor-alpha modulation of transcription of the mouse testis- and pre-implantation development-specific Rnf33⁄Trim60 gene pot
Ngày tải lên :
29/03/2014, 00:20
... Construction of luciferase reporter plasmids and site-specific mutagenesis The 2560 -bp F1 genomic fragment was PCR-amplified from the mouse genomic BAC45 clone [2] using the DyNAzymeÔ II Hot Start ... POZ gene family: impact of evolutionary route on gene expression pattern Gene 460, 39–47 26 Fablet M, Bueno M, Potrzebowski L & Kaessmann H (2009 ) Evolutionary origin and functions of retrogene ... HH, Liu TY, Li H & Choo KB (2002 ) Use of a common promoter by two juxtaposed and intronless mouse early embryonic genes, Rnf33 and Rnf35: implications in zygotic gene expression Genomics 80,...
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Báo cáo khoa học: Adeno-associated virus gene transfer in Morquio A disease – effect of promoters and sulfatase-modifying factor 1 pot
Ngày tải lên :
29/03/2014, 21:20
... Beldjord K et al (2008 ) Insertional oncogenesis in patients after retrovirus-mediated gene therapy of SCID-X1 J Clin Invest 118, 3132–3142 36 Edelstein ML, Abedi MR & Wixon J (2007 ) Gene therapy clinical ... cells Hum Gene Ther 8, 575–584 46 Stoneley M & Willis AE (2004 ) Cellular internal ribosome entry segments: structures, trans-acting factors and regulation of gene expression Oncogene 23, 3200 3207 ... E, Hoshika A & Ozawa K (2004 ) Adeno-associated virus (AAV) vector-mediated liver- and muscle-directed transgene expression using various kinds of promoters and serotypes Gene Ther Mol Biol 8, 9–18...
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Báo cáo khoa học: Cloning and functional analysis of 5¢-upstream region of the Pokemon gene pptx
Ngày tải lên :
30/03/2014, 04:20
... deletion constructs of the upstream region of the Pokemon gene The 2204 -bp upstream region of the Pokemon gene, which spans the region )2220 to )17, was amplified by PCR from human blood genomic ... the region from )837 to )560 and negative elements in the region from )560 to )233 FEBS Journal 275 (2008 ) 1860–1873 ª 2008 The Authors Journal compilation ª 2008 FEBS 1861 Analysis of upstream ... of the Pokemon gene, a 2204 -bp section of the 5¢ -upstream region of the Pokemon gene was cloned by PCR using human genomic DNA as the Analysis of upstream region of the Pokemon gene template Figure...
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Báo cáo sinh học: "Alpha-1 antitrypsin protein and gene therapies decrease autoimmunity and delay arthritis development in mouse model" potx
Ngày tải lên :
18/06/2014, 19:20
... Representative joint section from mice receiving hAAT gene therapy (E,F) Representative joint section from mice in control group (saline injection) Magnification: C,E: 100x; D,F: 200x Grimstein et al ... viruses from rhesus monkeys as vectors for human gene therapy Proc Natl Acad Sci USA 2002 , 99:11854-11859 Wu Z, Asokan A, Samulski RJ: Adeno-associated virus serotypes: vector toolkit for human gene ... adeno-associated virus-mediated alpha-1 antitrypsin gene therapy prevents type I diabetes in NOD mice Gene Ther 2004 , 11:181-186 Lu Y, Tang M, Wasserfall C, Kou Z, Campbell-Thompson M, Gardemann T, Crawford...
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