altered microrna expression in human heart disease
báo cáo khoa học: " High-throughput sequencing identifies STAT3 as the DNA-associated factor for p53-NF-κB-complex-dependent gene expression in human heart failure" pps
Ngày tải lên :
11/08/2014, 12:20
...
domain. Purified recombinant GST-RELA peptides (amino terminus, DNA
binding domain; carboxyl terminus, transactivation domain; in the left
panel, the arrow indicates the GST-RELA 428-551 amino ... multi-protein complex
may control gene expression program in heart failure.
Understanding the way that different DNA binding pro-
teins interact with DNA regulatory elements and modu-
late gene expression ... mir-21 expression. p53 behaves as a co-factor in this complex because despite a mutation
in its DNA binding domain, mutant p53 was still capable of binding RELA and the cis-element, and inducing...
- 14
- 244
- 0
Báo cáo sinh học: "Differentiation associated regulation of microRNA expression in vivo in human CD8+ T cell subsets" doc
Ngày tải lên :
18/06/2014, 19:20
... implication of microRNAs in CD8
+
T cell differentiation in humans,
microRNA expression profiles were analysed using microarrays and quantitative PCR in several human CD8
+
T cell
subsets defining the ... *
53 hsa-miR-30d
MicroRNA ranking according to relative expression in CD8
+
T cells. Groups A
and B are as identified by unsupervised clustering in Figure 1b. *: microRNA
belonging to the miR-17~92 ... few
highly expressed microRNAs prevailed in frequency.
Interestingly, these microRNAs include miR16, miR-21,
miR-142-3p and miR-142-5p, wh ich were also found in
the group A in human CD8
+
T lymphocytes,...
- 8
- 399
- 0
báo cáo hóa học:" Differentiation associated regulation of microRNA expression in vivo in human CD8+ T cell subsets" pptx
Ngày tải lên :
20/06/2014, 03:20
... implication of microRNAs in CD8
+
T cell differentiation in humans,
microRNA expression profiles were analysed using microarrays and quantitative PCR in several human CD8
+
T cell
subsets defining the ... *
53 hsa-miR-30d
MicroRNA ranking according to relative expression in CD8
+
T cells. Groups A
and B are as identified by unsupervised clustering in Figure 1b. *: microRNA
belonging to the miR-17~92 ... few
highly expressed microRNAs prevailed in frequency.
Interestingly, these microRNAs include miR16, miR-21,
miR-142-3p and miR-142-5p, wh ich were also found in
the group A in human CD8
+
T lymphocytes,...
- 8
- 326
- 0
Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt
Ngày tải lên :
23/03/2014, 09:21
... doxycycline-inducible protein expression by just a single transfec-
tion. pEBTetD contains an Epstein–Barr virus origin of replication for
stable and efficient episomal propagation in human cell lines, ... lines.
Several widely used systems for regulated expression
in mammalian cell lines are based on the tetracycline
Keywords
doxycycline; EpsteinBarr virus;
polyadenylation; regulated protein
expression; ... inducible protein expression M. Bach et al.
788 FEBS Journal 274 (2007) 783790 ê 2007 The Authors Journal compilation ê 2007 FEBS
Fast set-up of doxycycline-inducible protein expression in
human...
- 8
- 331
- 0
Báo cáo khóa học: Ouabain stimulates endothelin release and expression in human endothelial cells without inhibiting the sodium pump Robert Saunders and Georgios Scheiner-Bobis pptx
Ngày tải lên :
30/03/2014, 13:20
... but also in identifying several of them as ouabain or
its isomer [3–6] or as its congeners, such as digoxin [7,8],
proscillaridin A [9], 19-norbufalin [10] and marinobufagenin
[11]. In addition, ... 2; Worthing-
ton) in Pucks saline solution (Seromed, Berlin, Germany)
containing 20 m
M
Hepes, and incubated at 37 °Cfor
20 min to detach the endothelium. The cells were washed
out using 20–50 ... well.
86
Rb
+
uptake was also investigated after preincubating
the cells for 15 min with 1 l
M
protein kinase C inhibitor
Ro-31-8425, 5 l
M
Na
+
-channel inhibitor tetrodotoxin,
500 l
M
mitochondrial...
- 9
- 288
- 0
Báo cáo hóa học: " Altered gene expression in asymptomatic SHIV-infected rhesus macaques (Macacca mulatta)" pdf
Ngày tải lên :
20/06/2014, 01:20
... actin-binding genes appeared in the
list; namely: anillin (ANLN, ID: R16712
), destrin (DSTN,
ID: AA424824
), utrophin (UTRN, ID: AA676840), cyclin-
dependent kinase 2-interacting protein (CINP, ... (Telechem
Inc., OR) were used for printing the cDNA clones using 12
ì 4 pin format, on a Virtek chip writer professional micro-
arrayer in KemTek, Inc, MD. The printed slides underwent
UV cross-linking, ... free of clinical signs of SHIV, altered baseline
gene expression data may give clues to the pathogenesis of
altered immune response to secondary infections. Studies
involving HIV-infected humans...
- 8
- 260
- 0
Báo cáo khoa học: "Coxsackievirus and adenovirus receptor expression in human endometrial adenocarcinoma: possible clinical implications" doc
Ngày tải lên :
09/08/2014, 07:21
... according to CAR positivity (positive vs nega-
tive CAR staining), level of expression (high vs low level of
CAR expression) and staining intensity (mild vs moderate
and intense CAR staining intensity) ... CAR
protein by immunohistochemistry, while the potential
Intense immunostaining for CAR in tumor cells in representative endometrial adenocarcinoma cases (original magnification ì200)Figure 1
Intense ... while the remain-
ing 15 patients were remained disease free. All the exam-
ined clinicopathological parameters are reported in Tables
1, 2 and 3.
Immunohistochemistry
Immunostainings for CAR...
- 8
- 294
- 0
Báo cáo khoa học: " Assessment of Epidermal Growth Factor Receptor (EGFR) expression in human meningioma" docx
Ngày tải lên :
09/08/2014, 09:20
... the sample exhibiting staining); 3 (41-60% of the sample exhibiting staining); 4 (61-80% of the
sample exhibiting staining), and 5 (81-100% of the specimen stained). The findings of the immunoreactivity ... cells staining for
EGFR and histopathologic subtype.
What does overexpression of EGFR in tumors indicate?
Carroll et al provide an explanation by examining EGFR
expression in human meningiomas ... only
in their behavior but also in their expression of EGFR
[33]. Lusis et al in a report evaluating the expression of
EGFR in 41 meningiomas identified a relatively higher
incidence of EGFR expression...
- 7
- 302
- 0
Báo cáo y học: "Tumor necrosis factor alpha and adalimumab differentially regulate CD36 expression in human monocytes" ppt
Ngày tải lên :
09/08/2014, 10:20
... anti-inflammatory properties are used in human ther-
apy, regulate in vitro CD36 expression. Aspirin increases
CD36 expression in lines of human THP1 macrophages [23],
dexamethasone induces CD36 expression ... expression in human dendritic
cells from healthy subjects [24] and atorvastatin increases
CD36 expression in human monocytes [36,37].
According to the results of our study, the increase in mem-
brane expression ... conditions.
Mechanisms involved in the regulation of CD36
expression by TNFα
Since PPARγ is a transcription factor that plays a key role in
inducing membrane expression of CD36 on human mono-
cytes [19], we investigated...
- 11
- 471
- 0
Báo cáo y học: "Genome-wide assessment of imprinted expression in human cells." potx
Ngày tải lên :
09/08/2014, 22:24
... recogniz-
ing imprinting in mice using the non-equivalence of
monoallelic expression in reciprocal mati ngs of inbred
strains but yi elded widely different esti mates of amounts
of imprinted genes in ... parent-of-origin-determined expression. We also detected five novel
imprinted genes and delineated regions of imprinted expression surrounding annotated imprinted genes. More
subtle parent-of-origin-dependent ... ,837 windows measured in the fibroblasts, a total
of 549 were showing hig h AE. These candidate windows
fell into 254 distinct loci in LCLs and into 110 loci in
fibroblast s (Tables S5 and S6 in...
- 14
- 397
- 0
Altered neuronatin expression in the rat dorsal root ganglion after sciatic nerve transection ppsx
Ngày tải lên :
10/08/2014, 05:21
... overexpression of Nnat in HAECs or in
mouse carotid artery would induce increased expression
of a panel of nuclear factor-kappaB (NF-κB)-regulated
genes, including inflammatory cytokines, chemokines,
and ... nerve in adult
rats induced an increased expression of Nnat in DRG
neurons, specifically in unmyelinated C-fiber and thinly
myelinated A-δ fiber neurons, the primary afferent neu-
rons involved in ... imprinting gene strongly
expressed mainly in neonatal developing brain, increased
its expression in adult rats DRGs after axotomy of sciatic
nerve. Although it is not clear why Nnat expression was
increased...
- 7
- 207
- 0
báo cáo khoa học: " Microarray-based analysis of microRNA expression in breast cancer stem cells" doc
Ngày tải lên :
10/08/2014, 10:20
... prepared f or H&E staining
and immunohistochemical staining. The tumors in the
BCSCs group were positive for both EMA and SMA,
indicating that they were included of both luminal
epithelial and ... 26(29):4209-4215.
27. French J, Stirling R, Walsh M, Kennedy HD: The expression of Ras-GTPase
activating protein SH3 domain-binding proteins, G3BPs, in human
breast cancers. Histochem J 2002, 34(5):223-231.
28. ... Volinia S, Nenci I, Calin GA, Querzoli P, Negrini M, Croce CM:
MicroRNA gene expression deregulation in human breast cancer. Cancer
Res 2005, 65(16):7065-7070.
8. Gregory RI, Shiekhattar R: MicroRNA...
- 8
- 333
- 0
Báo cáo y học: "Altered protein expression in serum from endometrial hyperplasia and carcinoma patients" pdf
Ngày tải lên :
10/08/2014, 21:23
... carcinoma; HP: haptoglobin; HRG: histidine-rich
glycoprotein; IGFBP-4: insulin-like growth factor-binding protein 4; IHRP:
inter-alpha-trypsin inhibitor family heavy chain-related protein; IPI:
international ... these
proteins, including serum amy loid A (SAA), apolipopro-
tein A-I V (ApoA4), antithro mbin III (synonymous with
SERPINC1), and inter-alpha-trypsin inhibitor heavy chain
H4 (ITIH4; synonymous with inter-alpha-trypsin ... 2010,
17:47-55.
33. Weiderpass E, Brismar K, Bellocco R, Vainio H, Kaaks R: Serum levels of
insulin-like growth factor-I, IGF-binding protein 1 and 3, and insulin and
endometrial cancer risk. Br J Cancer 2003,...
- 8
- 258
- 0
