again the math is ok but this time what about some applications of c gc ms itd
Báo cáo y học: " GM-CSF and IL-2 as adjuvant enhance the immune effect of protein vaccine against foot-and-mouth disease" pot
... GAA TTC CAA GTC ATT GTT GAG TAG ATG C 3’ BoGM-CSF F 5’ CTA GAA TTC GCA CCT ACT CGC CCA CCC AA 3’ BoGM-CSF R 5’ TTA CCG CGG CTT CTG GGC TGG TTC CCA G 3’ VP1 F 5’GCA CCG CGG ACC ACC TCT GCG GGT ... GAG TCT 3’ VP1 R 5’GAC CTC GAG CAG AAG CTG TTT TGC GGG T 3’ VP1 F1 5’GCA GAA TTC ACC ACC TCT GCG GGT GAG TCT 3’ VP1 R1 5’GAC CTC GAG CAG AAG CTG TTT TGC GGG T 3’ BoGM-CSF VP1 by the identification ... CCA GAC TTT GTT G GAG GGT AGG CTG GCC TAT GGC T 352 bp [28] IL-2 5’ TCC ACT TGA AGC TCT ACA G 3’ GAG TGA AAT CCA GAA CAT GCC 247 bp IFN-g 5’ CAT TGA AAG CCT AGA AAG TCT G 3’ CTC ATG GAA ATG CAT...
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Báo cáo y học: "Treatment of hypertriglyceridemia and HIV: fenofibrate-induced changes in the expression of chemokine genes in circulating leukocytes" ppsx
... endothelial cells, which, in combination with the CCR2 decreased expression, could be beneficial with respect to the risk of atherosclerosis considering their well documented role in the local accumulation ... Watanabe MA: Genetic polymorphisms in the chemokine and chemokine receptors: impact on clinical course and therapy of the human immunodeficiency virus type infection (HIV-1) Curr Med Chem 2007, 14:1325-1334 ... significant effect of fenofibrate in the MCP-1/CCL2 gene expression However, there was a significant decrease in the gene expression of the CX3CL1/ Fractalkine in patients treated with fenofibrate...
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Báo cáo khoa học: Hydrogen independent expression of hupSL genes in Thiocapsa roseopersicina BBS pot
... gene, the 1618 bp PCR fragment obtained with primers rpoN1 (5¢-GCTGC ATCTCGACGATCTTC-3¢) and rpoN2 (5¢-ATCGCTTGC GCTGAGCCTCT-3¢) from rpoN (GenBank Accession Number AY837592) was inserted into the ... primers speci c for the hynS gene: otsh14 (5¢-GAT CGCGATATTGAACATC-3¢) was used in the reverse transcription and otsh11 (5¢-CTGCCCGAGCTTGACGC-3¢) served as other primer in PCR Using these primers ... This This This This work work work work [35] Stratagene Promega This work Stratagene This work [10] [10] [28] [29] [36] GenBank no L05081 This work This work This work This work [37] [15] This...
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expression of recombinant genes in eukaryotic systems
... starting concentration of 5-20 nM This induces a severe crisis associated with cell death, which can last 3-4 weeks, until by outgrowth of cell islets resistant to this concentration the tissue culture ... two other facts contributed significantly to the success of the system The availability of the entire sequence of the AcNPV virus 64 has allowed identification and characterization of many hitherto ... hu-LIF could be detected (Fig 1), reflecting incomplete glycosylation as a consequence of incomplete processing, possibly in conjunction with the compromised state of the insect cell at the time of...
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Báo cáo sinh học: "Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI" potx
... which it was digested with Bgl II and BamH I The EMCV IRES was amplified from pIRES2-eGFP (Clontech) using the oligonucleotides TACCGCGGGCCCGAGATCTGCCCCTCTC and CCGGATCCCATGGTTGTGGCCATATTATCA, ... previously described [25] In brief: (i) HOXB4-2A-MGMTP140KIRES-eGFP HOXB4 was amplified from its cDNA using the oligonucleotides TTGCGGCCGCCATGGCTATGAGTTCTTTTTTGATC and TTCTCGAGAGAGCGCGCGGGGGCCTC, following ... restriction fragment (ii) MGMTP140K-2A-HOXB4IRES-eGFP MGMT was amplified from its cDNA using the oligonucleotides TTGCGGCCGCCATGGACAAGGATTGTGAAATG and TTCTCGAGAGTTTCGGCCAGCAGGC, following which...
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báo cáo hóa học:" Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI" ppt
... which it was digested with Bgl II and BamH I The EMCV IRES was amplified from pIRES2-eGFP (Clontech) using the oligonucleotides TACCGCGGGCCCGAGATCTGCCCCTCTC and CCGGATCCCATGGTTGTGGCCATATTATCA, ... previously described [25] In brief: (i) HOXB4-2A-MGMTP140KIRES-eGFP HOXB4 was amplified from its cDNA using the oligonucleotides TTGCGGCCGCCATGGCTATGAGTTCTTTTTTGATC and TTCTCGAGAGAGCGCGCGGGGGCCTC, following ... restriction fragment (ii) MGMTP140K-2A-HOXB4IRES-eGFP MGMT was amplified from its cDNA using the oligonucleotides TTGCGGCCGCCATGGACAAGGATTGTGAAATG and TTCTCGAGAGTTTCGGCCAGCAGGC, following which...
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Báo cáo y học: " Comparison of the expression of cytokine genes in the bursal tissues of the chickens following challenge with infectious bursal disease viruses of varying virulence" pps
... AF000631.1 IL-13 CTGCCCTTGCTCTCCTCTGT CCTGCACTCCTCTGTTGAGCTT Forward GCTGAGGGTGAAGTTTGAGGAA Reverse IL-10 Forward Reverse GAAGCGCAGCATCTCTGACA H strain Forward CACTCCCTGGTGGCGTTTA 126 AY321955.1 ... real -time PCR Genes Direction Sequence Product (bp) Accession no in GenBank GAPDHa Forward TGCCATCACAGCCACACAGAAG 123 AF047874.1 IFN-ga Reverse Forward ACTTTCCCCACAGCCTTAGCAG AAGTCAAAGCCGCACATCAAAC ... Reverse GACCGTATCATTTGCCCATTG IL-4 Forward AATGACATCCAGGGAGAGGTTTC 100 AJ621249.1 IL-5 Reverse Forward AGGCTTTGCATAAGAGCTCAGTTT GGAACGGCACTGTTGAAAAATAA 111 AJ621252.1 Reverse TTCTCCCTCTCCTGTCAGTTGTG...
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Differential expression of cellular genes during a west nile virus infection
... Another common outcome of virus-host interaction is the physiological process of cell death Apoptosis, which is an active and highly conserved process of cellular self-destruction with distinctive ... clusters can be decided by the user, and the algorithm then randomly assigns each gene to one of the K clusters The distance between each gene and the centre of each cluster (centroid) is calculated, ... specific locations on a solid support, such as a coated glass surface Arrays allow the identification of the sequence, and the abundance of each detected nucleic acid interrogated by the microarray...
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Expression of EBV genes in nasopharyngeal carcinoma
... cells infection is occurred as the consequence of local reactivation of the virus from EBV-carrying B lymphocytes This hypothesis is based on the observation: EBV was found in B-lymphocytes but not ... EBNA 3C can increase the production of LMP1 in the presence of EBNA2 (Zhao et al., 2000) Further researches on EBNA 3C show it may disrupt multiple cell cycle checkpoints and induce nuclear division ... Etiology NPC often occurs in specific race and area This suggests that genetic and environmental factors may contribute to the oncogenesis of NPC Until now, many studies reveal that genetic factor,...
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Báo cáo hóa học: "A mutated xylose reductase increases bioethanol production more than a glucose/xylose facilitator in simultaneous fermentation and co-fermentation of wheat straw" docx
... Fermentation of mixed glucose-xylose substrates by engineered strains of Saccharomyces cerevisiae: role of the coenzyme specificity of xylose reductase, and effect of glucose on xylose utilization Microb ... participated in the design of the study and commented on the manuscript All authors contributed to the scientific discussion throughout the work and have read and approved the final manuscript Competing ... product formation from xylitol to ethanol The current comparison using isogenic S cerevisiae CEN.PK strains was undertaken to elucidate the relative contribution of these two beneficial genetic...
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Báo cáo sinh học: "Co-regulation of mouse genes predicts function" pps
... Hughes His team chose to start with a single source, the XM sequences from NCBI (see Table for a list of the resources mentioned in this article) “We downloaded the XM collection from the NCBI It’s ... the attributes of gene products in terms of Molecular Function, Biological Process and Cellular Component still undecided about how many genes make up a mouse “There is no ‘gold standard’ cDNA database ... detectable on the arrays,” says Hughes The collection contains about 75% of the current RefSeq sequences, it contains the majority of Ensembl genes, Journal of Biology 2004, 3:19 http://jbiol.com/content/3/5/19...
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Báo cáo lâm nghiệp: "doubling atmospheric CO concentration on 2 matter distribution and CO exchange of 2 yr growth, dry 2 old sweet chestnut trees (Castanea sativa Mill.)" pptx
... shorten the time period over which the main branches grew After the end of July, 62% of the enriched plants ceased growth as compared with 37% of the controls The early onset of yellowing and the ... growth indicates earlier senescence of the -enriched C0 plants The final leaf area per plant, at the end of the growth period, was reduced by C0 enrichment The pattern of growth through the vegetative ... reduction in shoot growth, which is very unusual and may have resulted from the timing of the nutrient application The accumulation of the carbon surplus was restricted to the root system These changes...
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Báo cáo y học: " Genome-wide comparative analysis of the Brassica rapa gene space reveals genome shrinkage and differential loss of duplicated genes after whole genome triplicatio" potx
... on the same chromosomes of Br However, we did not determine precisely which copy of the replicated AK block family corresponds to the Br BACs because of the possibility that Br sequences in the ... amplified bands, which could result in inaccurate mapping of the BAC sequence In this case, the resulting assignment of the BAC to an incorrect linkage group on a specific AK block family member ... gene-rich seed BACs as well as the alignment of sequence scaffolds Quick selection of a sufficient number of gene-rich seed BACs and directed ordering of the sequence scaffold will likely accelerate...
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báo cáo khoa học: " Molecular characterisation and genetic mapping of candidate genes for qualitative disease resistance in perennial ryegrass (Lolium perenne L.)" doc
... figures and the primary drafts of the manuscript, and contributed to finalisation of the text and journal-specific formatting NC co-conceptualised the project and contributed to data analysis and ... location and mapped locus coordinate (cM) [p150/112 population] LpLrK10.1 LpPcaClone 1.1 LpPcaClone 1.2 LpPCAClone2.1 LpPcaClone3.1 LpPcaClone3.2 LpPcaClone3.3 LpPcaClone4.1 LpHvclone1 LpHvclone2 ... LpPcaClone1 LpPcaClone2 LpPcaClone3 LpPcaClone4 Oat NBS-LRR candidate from Pca cluster Oat NBS-LRR candidate from Pca cluster Oat NBS-LRR candidate from Pca cluster Oat NBS-LRR candidate from Pca...
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báo cáo khoa học: " An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development" docx
... protein CTTGCATCCCTCAGCACCTT/ TCCTGTGGACAATGGATGGA 82/2.00 GGTTCCCATGTTTACAGCATCTG/ GCACCCACTCAACGGTATTGTAC TCCGCCAAGGACTATCATGAC/ CGCAATCAGAGCCCTGTAGAA GGAGCCTGAGCCTACCTTCTC/ GTGTTCGGCCAGGTGGTAGA ... TCGTGATGCTGCTGCTAACAA/ TTGCCCAGTCAGGACCAAAT 62/1.90 CAACATCCTTTACCCATTGACAGA/ GCATTTGATCCACTTGCAGATAAG GGATAACTTCCCTGCCTCAATGA/ TTCTTGTAGCAGCTGAGAGGATCA CATGCGAGTGTCCCTCAATCT/ TCTCTTGCGTTTCTGGCTTAGA CAGCCAGATCTTCACGAGCTT/ ... CAGCCAGATCTTCACGAGCTT/ GTTCTCGCGCATTGACCATA TGAACCACTTGATCTCTGCGACTA/ CAGCTTGCGGAGGTCTGAGT GAGGGTCGTCAGGATTTGGA/ GCCCTGCACTTACCATCTTTAAG CATAACATTTGCGGCAGATCA/ TGGTGGTATTATTGAGCCATCCTT CGCCTGTCAATCTTGGTCAGTAT/...
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Cloning, characterisation and functional analysis of horseshoe crab c reactive proteins
... Sequence CrCRP-2F1 5’-TAAACGAATTCACTAGAGGAAGGTGAA-3’ CrCRP-2F2 5’-AGAATTCCAAGGCCTCACTTCAT-3’ CrCRP-2F3 5’-AGAATTCCTGTCACACGTGGTCA-3’ CrCRP-2F4 5’-AGAATTCCTGTGTGCATCATTCG-3’ CrCRP-2R 5’-AACAGCTCGAGGAACAGTGAAAAATTC-3’ ... T 61 21 TTACCTGATCTGCAAGAAATTACCTTATGTTACTGGTTCAAGCTGCATCGCTTAAAGGGC L P D L Q E I T L C Y W F K L H R L K G 121 41 ACACCTCATATATTTTCTTACGCCAACTCTGAAACAGACAATGAGATTCTGACATCTCTG T P H I F S Y ... CrCRP-2F-1Æ TCTAACTTCTGCTCTAGAGGAAGGTGAAATCAGCACAAAGGTTAAATTTCCTCCGTCTAG L T S A L E E G E I S T K V K F P P S S 121 41 TTCTCCGTCATTCCCGCGACTAGTAATGGTGGGAACGTTACCTGATCTGCAAGAAATTAC S P S F P R...
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Báo cáo y học: "otifs and cis-regulatory modules mediating the expression of genes co-expressed in presynaptic neurons" ppsx
... P-value Cis-regulatory module scoring metric Given the 16 motifs enriched in cluster MCEs, we hypothesized that the co-occurrence of some of these motifs in an MCE may be indicative of the MCE's ... the likelihood ratio of the probability of the motif not occurring by chance to the probability of the motif occurring by chance Let p(x) be the P-value of motif x Thus, for an MCE having occurrences ... function that scores each MCE as a sum of the likelihood-ratio of enrichment for each of the 16 motifs if they are present in the MCE We ranked 3,347 MCEs by their scores representing the MCE's...
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Tài liệu Báo cáo khoa học: Molecular characterization, phylogenetic relationships, and developmental expression patterns of prion genes in zebrafish (Danio rerio) doc
... pair (sense oligonucleotide ZFC1, 5¢-CAGATTCTCAGTCCACGA-3¢ and antisense oligonucleotide ZFC2 5¢-GCTCACTGTT TCCTCATCC-3¢) was used to amplify a 606 bp PrP1 cDNA fragment from nucleotides 1003–1608 ... oligonucleotide ZFB1 5¢-AGG CAGCAGCTAGGGTC-3¢ and antisense oligodeonucleotide ZFB2 5¢-GTCCAAACACTGTCACGC-3¢) was used to amplify a 401 bp PrP3 cDNA fragment designed from nucleotides 129–529 in the ... that matched with the predicted secondary structures of human PrPC (Fig 2) Hydrophobic cluster analysis [27] predicted the presence of several conserved hydrophobic clusters throughout the compared...
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Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx
... GACTGTTGCTCATTTGTGGA GAGGCTAGATACTGCTCGATGT TGATGATTTGGAACCATTATTGAA CACCTTTTTCCTTCATCTTTTCAT ACTACCTCATGAAGATCCTG TTGCTGATCCACATCTGCTG TAATACGACTCACTATAGGG ATTTAGGTGACACTATAGAA and at 72 C, with ... highly conserved regions, and amplified product gave a speci c product of 284 bp A set of b-actin primers (forward: 5¢-ACTACCTCATGAAGATCCTG-3¢ and reverse: 5¢-TTGCTGACCACATCTGCTG-3¢) served as a control ... presented as PCR products after normalizing against products of b-actin The x-axis indicates the time- periods of LPS incubation and the relative expression of the carp IL-10 gene on the y-axis Controls...
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Tài liệu Báo cáo Y học: Purification, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase pot
... determined by cytochemical staining [33–36] Besides oviduct and liver, the chicken ectoATPDase is also present in the apical membranes of the oxyntic-peptic cells [37] The distribution of the ectoATPDase ... antibody, MC27, the protein could be seen to be distributed at the bile canaliculi (Fig 4) This localization of the ecto-ATPDase agrees with previous finding of distribution of cell surface ATPase activity ... hydrolysis activity in the presence of Ca2+ [26], the CaATPase activity of the purified chicken liver ecto-ATPDase is 30% of the MgATPase activity at a divalent ion-ATP concentration of mM at...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008