... NaCl, and 1mm dithiothreitol, and used for further analyses. The concentration ofthe purified protein was determined from the extent of UV absorption with A2800:1%values of 0.83 for the full ... Fig. 3, the full length protein andthe N-ter-minal domain of SCO2299 could cleave the RNAstrand ofthe RNAÆDNA hybrid but the C-terminalregion could not. This result agreed with that of the in ... byligating the DNA fragment containing the full length, the RNase H domain, or the APase domain ofthe SCO2299gene to the NdeI–BamHI site of pET-11a. The DNA frag-ments were amplified by PCR using...
... miss the fact that the buildings on the right side ofthe image are much taller than those on the left. Also of note are the converging lines ofthe image. They make the buildings look as if they ... emulate the shot, you will beemulating only the subject and roughly, at that. The other elements ofthe photograph—light,color, shades of gray—are up to you to refine. The symbolism ofthe photos ... middle left ofthe frame. In the right side ofthe frame, the valley extends to a far-off mountain range, which sits on the horizon in haze, probably dueto the marine layer, which sits off the coast...
... listed on the Oslo Stock Exchange (OSE), the number of newbank relationships, the number of terminated bank relationships, the number of firms listing and delisting from the OSE, andthe total ... beginning ofthe crisis in1988. DEBT is the book value of debt, divided by the sum of year-end market value of equity and bookvalue of debt. DRAWN CREDIT is the book value of drawn lines of credit, ... dismissal ofthe boards of directors and top management at both Fokus and Christiania Bank.12 Third, the impact ofthe crisis on the banking industry has been long-lasting. As of September 2000, the...
... reduces the pep-tide binding affinity by 100-fold andthe lifespan of the complex by 10-fold, as judged by koff. The physiologicalchaperone cycle probably consists ofthe rapid binding of the ... hypothesize that the proton-ated state ofthe chaperone initiates this cycle, whereas the deprotonated state occurs upon completion of the maturation process ofthe partner. The nature of the signal ... common feature of this newfamily of chaperones. It is worth mentioning that the function of these proteins is not restricted to the recog-nition and binding ofthe N-terminus ofthe nascentmetalloprotein,...
... are “working in the trenches” of building the web. Most ofthe people in this latter segment of responders came to the survey by invitation because they are on the email list ofthe Pew Internet ... attendance of seminars and (some) lectures. The length ofthe learning period (the traditional four-year degree) may change as a result ofthe focus on combined learning, with integration of more off-site ... classroom time. The idea of having students watch the video lecture and/ or read the material at home and then work on problems or case studies together in the classroom with other students and a teacher...
... nm, the b-band at520 nm andthe a–band at 550 nm [18]. The deviations of approximately 3 nm between the signals of horse heartcytochrome c andthe heme c in the caa3-oxidase from T. thermophilus ... with the oxidized andthe negativesignals with the reduced form ofthe enzyme. For the reduced form, the Soret band can be observed at 415 and 442 nm, and for the oxidized form at 403 and 422 ... presented and on this basis the contributions ofthe reorganization of the polypeptide backbone, of individual amino acids andof the hemes c, a and a3upon electron transfer to /from the redoxactive...
... kinsmen, the golden-wing prefers the fields andthe borders of the forest to the deeper seclusion ofthe woods, and hence, contrary to the habit of his tribe, obtains most of his subsistence fromthe ... other. One ofthe females then rushed, apparently with notes of joy and congratulation, to one ofthe males, who gave her a spiteful tweak and blow. Then the other came and he served her the ... immensely, and away the two go in quest of material for the nest, the male acting as guard and flying above and in advance[2] ofthe female. She brings all the material and does all the work of building,...
... perform the bulk of adver-tising and marketing in their con-versations with friends and ac-quaintances (Corum, 1999). En-courage frequent shoppers to bring their friends and family and offer them ... suffer. There are also interdependent partnerships between the market and shoppers, media, local business andthe community as a whole. When we ignore anyone of connections, the market and its ... posters in their windows and offer to distribute store coupons good only on market day. And finally the market’s partnership with its shop-pers. Convenience is the buzzword ofthe day—grocery...
... siteto the C-terminal segment ofthe repeat. The distinct localization of the 15E11 epitope andthe fibronectin-binding site suggests that the interferingeffect ofthe antibody might result from ... binding of the ligand to the other repeats that share a common epi-tope. Other inhibition mechanisms cannot be excluded;among these, there is the possibility that, through the effect ofthe proximities ... proximities ofthe antibody-binding and Fn-binding sites, the interaction ofthe repeat with Fnmay be sterically hindered in the presence ofthe anti-body. The results of this study allow us to draw several conclusions....
... experimentally. Indeed, the presence of Arg213 along with the proximity ofthe chargerelay Asp260 within the active site could be responsible for the modification ofthe catalytic properties ofthe catalytichistidine. ... located within the Ônucleophilic elbowÕ connectingstrand b5 and helix a3, while His290 and Asp260 e merge from the b8-a8andb7-a7 loops, respectively. Anotherimportant feature ofthe catalytic ... avor the displacement ofthe equilibriumtoward the neutral state of His290.To attest this hypothesis, an Arg213Ala mutant has beenexpressed and characterized. The specific activity of the Ala213...
... 5B). One of its aminogroups is about 2.6 A˚ from the Oc atom of Ser171 and 2.5 A˚ from the thiol group of Cys145.Even taking into account all ofthe limitations of molecular models of proteins ... on the basis ofthe homology model, as the increased occupancy deriving fromthe arginine sidechain in position 96 might modulate the size of the cleavable substrates (Fig. 4). Furthermore, the ... mutagenesis and their activity assayed with the colorimetric method of Berthelot, appropriately modified for the assay con-ditions ofthe archaeal enzyme. This assay is based on the measurement of the...
... process of inquiry and develop an enhanced understanding ofthe nature and methods of science, and (3) to recognize the role Figure 11 Conceptual Flow ofthe Activities of science in society and ... students see they can extend and apply their understanding of cancer to the real world. It also is important that they receive feedback on the adequacy of their explanations and understandings. ... like the limbs of a crab. From that observation came the term karkinoma in Greek, and later, cancer in Latin. With the work of Hooke in the 1600s, and then Virchow in the 1800s, came the understanding...
... epithelial SCs. The first evidence to support the notion of mammary SCs came from murine transplantation experiments. Mammary gland tissue was re-moved from a donor mouse and transplanted into the ... 10%–15% ofthe cells within the epithelium coexpress oestro-gen receptor alpha (ERα) and progesterone receptor (PR) and are knownto be located in the luminal epithelia ofthe ductal and lobular ... in all aspects of female fertility (Lydon et al. 1996). The uterus is one ofthe major PR target organs and a major source of the infertility of PR–/–female mice. The uterus of PR–/–mice...
... analysis of costs alone andof costs and benefits, but we omit this step fromthe diagram because we do not conduct uncertainty analyses on the CGE modeling. The Benefits and Costs ofthe Clean ... 1-8 and other sector-specific emissions models developed and used in various offices of EPA's Office of Air and Radiation. These emissions models provide estimates of emissions of five ... and other areas ofthe economy. Further discussion ofthe CGE modeling conducted to estimate the impacts ofthe CAAA on net social welfare is presented in Chapter 8. This chapter consists of...
... properties ofthe FeFe protein (Eur. J. Biochem. 269) 1655Comparison ofthe turnover signals from enzyme-reduced states ofthe MoFe protein andthe FeFe protein The characteristic EPR signal ofthe ... stems from t he P3+cluster, then the g ¼ 2.27 feature derives fromthe FeFe c ofactor. On the other hand, if the broad signal, d espite its unusualproperties, represents the P1+cluster, the ... tube. They contained 2 4 m g Rc1Feper mL and 0.6 mg Rc2Feper mL in the case ofthe F e nitrogenase and 28 mgRc1Moper mL and 0.7 mg Rc2Moper mL in the case ofthe Monitrogenase. The other...