acute pancreatitis spinal cord injury
Báo cáo y học: "Tetra-O-methyl nordihydroguaiaretic acid (Terameprocol) inhibits the NF-B-dependent transcription of TNF-a and MCP-1/CCL2 genes by preventing RelA from binding its cognate sites on DNA" ppsx
... Quantitative RT-PCR analysis Total RNA was extracted using the RNAeasy kit (Qiagen, Valencia, CA) according to the manufacturer’s specifications Residual genomic DNA was eliminated using oncolumn ... final) for h at 45°C and DNA was purified using Qiaquick nucleotide removal columns (Qiagen) according to the manufacturer’s instructions ChIP Q-PCR and data analysis For realtime PCR, bound ... Note that levels of total IB-a drop below levels of detection at the 10 time point (Figure 6A) According to the antibody manufacturer, this occurs because phosphorylation of IB-a is complete and...
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Tài liệu Báo cáo khoa học: Solution NMR structure of five representative glycosylated polyene macrolide antibiotics with a sterol-dependent antifungal activity doc
... Biochem 269) 4537 Fig 1H NMR spectrum of nystatin A1, mM in MeOH-d4 recorded at 1H-500 MHz and 25 °C Resonance lines are labeled according to the hydrogen numbering indicated in Fig Asterisks indicate ... solutions were prepared under dry argon in methanol-d4 at 3–5 mM concentration All NMR spectra were recorded at 25 °C on a Bruker Avance DRX 500 spectrometer (1H ¼ 500 MHz) using a 5-mm (1H, 13C, 15N) ... Homonuclear two-dimensional spectra DQF-COSY [37], TOCSY (HOHAHA) [38,39] and ROESY [40,41], were recorded with a 1.5-s recovery delay in the phase-sensitive mode using the States-TPPI method [42]...
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Báo cáo khoa học: Characterization of the bga1-encoded glycoside hydrolase family 35 b-galactosidase of Hypocrea jecorina with galacto-b-D-galactanase activity pdf
... 2,4,6trihydroxyacetophenone (20 mgÆmL)1 in methanol) as the matrix Positive-ion mass spectra were recorded on a vacuum MALDI-TOF ⁄ curved field reflector TOF instrument (TOF2, Shimadzu Biotech, Manchester, ... technique for 2,5-dihydroxybenzoic acid (10 mgÆmL)1 in water) Positive-ion mass spectra were recorded on the same instrument as mentioned above External calibration was performed using an aqueous...
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Báo cáo khoa học: Lending a helping hand, screening chemical libraries for compounds that enhance b-hexosaminidase A activity in GM2 gangliosidosis cells pptx
... Tanowitz HB & Wittner M (1988) Pyrimethamine concentrations in serum and cerebrospinal fluid during treatment of acute Toxoplasma encephalitis in patients with AIDS J Infect Dis 157, 580–583 Leport ... form These are associated with less severe ‘late-onset’ forms of the disease (i.e juvenile ⁄ subacute or adult ⁄ chronic variants) The life expectancy of patients with the adult forms (ATSD or ... molecules The Maybridge library was chosen to be screened, because it consisted of 50 000 drug-like (according to Lipinsky’s rules) compounds that sampled a diverse chemical space and that can readily...
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Báo cáo khoa học: Selection of peptides inhibiting a b-lactamase-like activity docx
... stock solutions were stored at )20 °C and prepared in water or in 50 mM acetic acid solution according to their hydrophobic features Fig Enrichment of the CX7C library in phages recognizing the ... round, which were amplified and sequenced These peptides could then be gathered in 10 families according to their amino acid content (Table 2) Within each family, the peptides share the same sequence ... negatively selected peptides (Table 3), displaying the best signals in phage ELISA, were synthesized according to the following feature: NH2GACXnCGAAGAEKCONH2, with Xn corresponding to the residues of...
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Báo cáo y học: "Increased interleukin-17 production via a phosphoinositide 3-kinase/Akt and nuclear factor κB-dependent pathway in patients with rheumatoid arthritis" ppt
... hours of incubation, mRNA was extracted with RNAzol B (Biotex Laboratories, Houston, TX, USA) in accordance with the manufacturer's instructions Reverse transcription of µg of total mRNA was performed...
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Báo cáo y học: "Tumor necrosis factor-alpha promotes survival in methotrexate-exposed macrophages by an NF-B-dependent pathwa" pptx
... RAW264.7 cells (8 × 106 per treatment) that were treated with 10 ng/ mL TNF-a for up to one hour, according to previously described protocols [21] A double-stranded NF-B consensus oligonucleotide ... expression in transfected RAW264.7 cells was measured using the Promega Luciferase Assay System, according to the manufacturer’s instructions Promoter activity was quantified by measuring light production ... relative light units (RLU) BMDM were transfected with Lipofectamine™ and Plus reagents (Invitrogen) according to manufacturer’s instructions Briefly, for each 24-well, μg pNF-B-TALuc was combined with...
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Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt
... the spectrum at energies near 7113 eV reveals a forbidden 1s fi 3d electronic transition that, according to prior studies of nonheme Fe2+ centers [29–31], is assigned to the mixing of the 4p orbital ... varied from a few minutes (2,4-pentanedione) to 0.5 h (other substrates) quartz cuvette Data were recorded using a Protein Solutions DynaPro DLS instrument (Wyatt Technology Corporation, Santa Barbara, ... 0.6 mLÆmin)1, and a column temperature of 65 °C was used CD spectroscopy Far-UV CD spectra were recorded with a Jasco J-715 spectropolarimeter (Tokyo, Japan) at room temperature, using 0.02 cm and...
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Tài liệu Báo cáo khoa học: Regulation of cathepsin B activity by 2A2 monoclonal antibody docx
... of acute pancreatitis J Clin Invest 106, 773–781 Van Acker GJ, Saluja AK, Bhagat L, Singh VP, Song AM & Steer ML (2002) Cathepsin B inhibition prevents trypsinogen activation and reduces pancreatitis ... nonapeptide (Fig 3A) The Kd of 4.7 nm (v2 = 8) obtained by fitting the curves according to the Langmuir binding model (1 : 1) was in accordance with both the results obtained by SPOT analysis, which revealed ... nonapeptide CIAEPGYSP (300 RU), mimicking the epitope for 2A2 mAb The obtained sensograms were fitted according to the Langmuir binding model (1 : 1), yielding a Kd of 4.7 nM (B) Cathepsin B was pre-bound...
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Tài liệu Báo cáo khoa học: Dual P2Y12 receptor signaling in thrombin-stimulated platelets – involvement of phosphoinositide 3-kinase b but not c isoform in Ca2+ mobilization and procoagulant activity pdf
... an overall increase in Ca2+ integral with thrombin alone, but not with thrombin + ADP (Fig 5) Accordingly, with H89 present, the contribution of ADP ⁄ P2Y12 to the Fig P2Y12 enhances thrombin-induced ... thrombin-induced Ca2+ integral with an IC50 of approximately 10 nm and lm, respectively, which is in accordance with the known affinity of these compounds for the PI3-K catalytic subunits At these concentrations ... CaCl2 stock solution, after which InsP3 was added Fluorescence intensities were continuously recorded at 488 nm excitation and 526 nm emission wavelengths using an SLM-Aminco DMX-1100 spectrofluorometer...
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Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt
... A phase II study of BTI-322, a monoclonal anti-CD2 antibody, for treatment of steroid-resistant acute graftversus-host disease Blood 92, 4066–4071 Branco, L., Barren, P., Mao, S.Y., Pfarr, D.,...
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Tài liệu Báo cáo khoa học: The Alzheimer b-peptide shows temperature-dependent transitions between left-handed 31-helix, b-strand and random coil secondary structures doc
... also at low temperature Results Circular dichroism (CD) spectroscopy Temperature dependence We recorded CD spectra at varying temperatures for the full length Ab(140)-peptide, as well as the fragments ... as the CD spectra show little temperature variation (data not shown) The shape of the spectrum recorded at C in Fig 1H suggests antiparallel b-sheet secondary structure Molecular mass studies conrmed ... maximum is dependent on the sequence [13] we determined an individual [h]max for each series of recorded spectra From the spectral intensities at [h]max the PII population, xPII was estimated using...
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Tài liệu Báo cáo khoa học: Molecular characterization and allergenic activity of Lyc e 2 (b-fructofuranosidase), a glycosylated allergen of tomato pdf
... reverse transcribed (RT) with the First Strand cDNA Synthesis Kit (Amersham Pharmacia Biotech) according to the manufacturer’s instructions using lg total RNA for each transcription and the NotI-d(T)18 ... Allergenic glycoprotein Lyc e (Eur J Biochem 270) 1329 BigDye Terminators v3.0 CycleSequencing Kit according to the manufacturer’s instructions trolled food challenge) and showed positive reaction ... SDS/PAGE followed by staining with Coomassie Brilliant Blue and the protein content was determined according to Bradford using the Roti-Quant protein assay (Roth) Patients’ sera Serum samples were...
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Tài liệu Báo cáo Y học: Characterization and regulation of yeast Ca2+-dependent phosphatidylethanolamine-phospholipase D activity docx
... maintained on SC drop-out medium lacking histidine SC media were prepared from YNB essentially according to Rose et al [22] Where indicated, SC medium was supplemented with 75 lM inositol (I+)...
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Báo cáo khoa học: The N-terminal hybrid binding domain of RNase HI from Thermotoga maritima is important for substrate binding and Mg2+-dependent activity pot
... that from compost metagenome [12], and their geometrical configurations are well conserved [13] According to the crystal structures of the C-terminal catalytic domains of Bacillus halodurans RNase ... water molecules, a two-metal ion catalysis mechanism has been proposed for RNase H [14,16,17] According to this mechanism, one metal ion is required for substrate-assisted nucleophile formation ... and is commonly present at the N-terminal regions of eukaryotic type RNases H (RNase H1) [21] According to the crystal structure of the HBD of human RNase H1 in complex with the RNA ⁄ DNA substrate,...
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Báo cáo khoa học: Autocatalytic processing of procathepsin B is triggered by proenzyme activity doc
... N-terminal aminoacid sequence analysis Protein concentrations were determined from absorption spectra according to Pace et al [34] The active proenzyme concentrations were determined by activation and...
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Báo cáo khoa học: Analysis of the NADH-dependent retinaldehyde reductase activity of amphioxus retinol dehydrogenase enzymes enhances our understanding of the evolution of the retinol dehydrogenase family pot
... orientation is the distribution of charged amino acids at either end of the hydrophobic sequence According to the ‘positive-inside’ rule, the most positively charged flanking transmembrane segment ... (2002) The retinoic acid signaling pathway regulates anterior ⁄ posterior patterning in the nerve cord and pharynx of amphioxus, a chordate lacking neural crest Development 129, 2905–2916 ´ ` Dalfo...
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