accessing investors requires more nuance and interaction with intermediaries
Báo cáo khoa học: Domains of ERRcthat mediate homodimerization and interaction with factors stimulating DNA binding potx
Ngày tải lên :
31/03/2014, 09:20
... PIS (lanes 8–12) with increasing amounts of ERRc (0.06 lL in lanes and 7; 0.13 lL in lanes and 8; 0,25 lL in lanes and 9; 0.5 lL in lanes and 10; lL in lanes and 11; lL in lanes and 12) The ERRc–DNA ... tissue culture dishes, washed twice with NaCl/Pi, and harvested in 1.5 mL NaCl/Pi by gently scraping with a rubber policeman Cells were centrifuged with 300 g and the pellet was resuspended in ... stimulating effect is not induced by lipophilic ligand receptor interaction A comparison with the homologous receptors ERRa, ERRb, ERa, and ERb and a data base search in the nonredundant protein...
- 12
- 297
- 0
plant roots growth activity and interaction with soils
Ngày tải lên :
06/07/2014, 15:21
... Roots and the Biological Environment 6.1 Interactions of roots with soil organisms 6.1.1 Root–rhizosphere communication 6.1.2 Interactions with bacteria 6.1.3 Interactions with fungi 6.1.4 Interactions ... Growth, Activity and Interaction with Soils Peter J Gregory Copyright © 2006 Peter Gregory Chapter Plants, Roots and the Soil This book focuses on vascular plants and their interactions with soils ... other by Howard Taylor and Betty Klepper, which enthused me with the possibility of combining my interests in plants and soils by studying roots and their interactions with soil I quickly found...
- 340
- 309
- 0
Báo cáo khoa học: " Evolutionary and structural analyses of alpha-papillomavirus capsid proteins yields novel insights into L2 structure and interaction with L1" ppsx
Ngày tải lên :
12/08/2014, 04:21
... loop and the FG loop of L1 and specific proline-rich regions of L2 (Fig 3) Amino acids 105–120 within the L1 DE loop interact with one highly conserved and one completely conserved proline within ... regions of L2 may be involved in interaction with L1 Nonetheless, our L2:L1 structural interaction model had distinct similarities with the Polyomavirus VP2 interaction with VP1 [44] The Polyomavirus ... of DNA bound to L2 and the difficulty in determining L2 flexure within the pentameric form of L1 In this model the DE and FG loop of L1 are involved in interaction with L2 and these loops are...
- 11
- 365
- 0
Báo cáo y học: "Pharmacokinetics of epinephrine in patients with septic shock: modelization and interaction with endogenous neurohormonal status" pdf
Ngày tải lên :
13/08/2014, 18:22
... are the standard parameter value for a patient with the standard BW value and the power parameter estimate for BW, and Pi and BWi are the parameter and BW of the ith individual Graphical evaluation ... infused, and plasma hormone concentrations Parameter estimates were standardized for a mean standard covariate using an allometric model: Pi = PSTD × (BWi/BWSTD)θBW where PSTD and θBW are the standard ... pharmacokinetics and no saturation at high doses in patients with septic shock Only BW and severity of illness influenced epinephrine pharmacokinetics No interaction between exogenous epinephrine and endogenous...
- 8
- 334
- 0
Tài liệu Báo cáo khoa học: Delineation of exoenzyme S residues that mediate the interaction with 14-3-3 and its biological activity ppt
Ngày tải lên :
19/02/2014, 08:20
... acids at positions 424 and 427 not mimic phosphorylated serine motifs important for the interaction between 14-3-3 and ExoS Therefore, this interaction is more complex and must occur in another ... how the interaction between 14-3-3 and ExoS occurs, our pull-down analysis with GST-ExoS(LDL426– 428AAA) still strongly suggests that ExoS must utilize a strategy for its interaction with 14-3-3 ... anti-ExoS antibodies (D) and (E) Cells were lysed and samples were separated by SDS ⁄ PAGE Western blot analysis was performed on immunoblotted filters with anti-Ras (D) and with anti-Erk (E) antibodies...
- 9
- 525
- 0
Tài liệu Báo cáo khoa học: Plant a-amylase inhibitors and their interaction with insect a-amylases ppt
Ngày tải lên :
21/02/2014, 03:20
... with more success [73] More This inhibitor class was not classi®ed by CATH program bOrengo et al [154] c1, 2, and from CATH code represents mainly a helix, mainly b sheets and mixed a helix and ... known modes of a-amylase inhibition A standard colouring scheme is used with enzyme drawn in magenta and inhibitor helix, strand and coil drawn in red, cyan and yellow, respectively The calcium ... 1) and forms a separated folding unit, exclusively made of b sheet Eight of the 10 strands fold into a b sandwich structure with ÔGreek keyÕ topology The conservation of the interface of A and...
- 16
- 539
- 0
Tài liệu MECHANICAL PROPERTIES OF THE HEART AND ITS INTERACTION WITH THE VASCULAR SYSTEM ppt
Ngày tải lên :
22/02/2014, 09:20
... understanding of the heart as a muscular pump and of the interaction between the heart and the vasculature The concepts of contractility, preload and afterload are paramount to this understanding and ... bundles of myosin strands with each strand having a tail, a hinge and a head region The tail regions bind to each other in the central portion of the filament and the Page of 23 strands are aligned ... understanding and will be the focus and repeating theme throughout the text A sound understanding of cardiac physiology begins with basic understanding of cardiac anatomy and of the physiology of muscular...
- 23
- 578
- 0
Báo cáo khoa học: Ion-binding properties of Calnuc, Ca2+ versus Mg2+ – Calnuc adopts additional and unusual Ca2+-binding sites upon interaction with G-protein pdf
Ngày tải lên :
07/03/2014, 00:20
... generate the J-band (610–650 nm) and ⁄ or the c-band (480–510 nm) Upon binding to Calnuc, the dye displayed prominent J-band and c-band in both absorption and CD spectra (Fig 6A,B) Furthermore, Ca2+ ... b-crystallin and d-crystallin behave similarly (show only one band), whereas calmodulin and troponin behave like each other On the other hand, Calnuc and parvalbumin generate both the J-band and the c-band, ... and parvalbumin seem to complex with the dye similarly, and yield the J-band [25] As the dye ⁄ protein ratio is decreased, the J-band is lost in the former two proteins, yielding the b-band and...
- 18
- 333
- 0
Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt
Ngày tải lên :
07/03/2014, 16:20
... for antibodies against SSA by ELISA with negative results, and diluted with RPMI 1640 or NaCl/Pi (1 : 1, v/v) Blood samples were taken with the understanding and written consent of each subject ... was gently treated with DTT and the resulting free Cys residues were alkylated with iodoacetamide As shown in Fig 1B, reduction and alkylation produced only one band in SDS/PAGE with the expected ... lM) and SSA (C) and SSA(C26S) (D) Scatchard analysis for the binding of Vb5.2–SSA with a Kd ¼ 10.04 lM (E) and Vb5.2–SSA(C26S) with a Kd ¼ 10.72 lM (F) Table Binding parameters for SAg–TCR interactions...
- 9
- 485
- 0
Báo cáo khoa học: tmRNA from Thermus thermophilus Interaction with alanyl-tRNA synthetase and elongation factor Tu pptx
Ngày tải lên :
08/03/2014, 08:20
... tRNA and tmRNA alanylation under standard reaction conditions and 50 °C should be close to 0.8 s)1 and 0.03 s)1, respectively This is to be compared with kcat values of 0.93 s)1 [27], 1.1 s)1 and ... mobility shift study of the interaction between EF-Tu*GDPNP and uncharged tmRNA Two equivalent gels were run at identical conditions and stained with Coomassie Blue R250 (A) and with pyronin Y enhanced ... the interaction with similar structural patterns (the alanylated acceptor stem with the specifically recognizable GU base pair, and the T loop) On the other hand, in an established complex with...
- 13
- 380
- 0
Báo cáo khoa học: Crystal structure of RNase A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitor potx
Ngày tải lên :
22/03/2014, 16:21
... maps, with the o and coot programs [36,37] The final refinement was performed with refmac from the ccp4 suite [32], with TLS parameterization Both cns and refmac used the same Rfree set (randomly ... dithiothreitol and mm EDTA, and purified further by anion exchange chromatography with a Mono Q column (Amersham Biosciences; 20 mm Tris ⁄ HCl, pH 7.5, containing 10 mm dithiothreitol and mm EDTA, with ... g) and 20 °C RI - RATE complex RI - RNase A complex RI Fig Analysis of the stoichiometry of the RI–SGRSGRSG-RATE complex by native PAGE Lanes and 2: complex of RNase A (100 pmol) with RI (200 and...
- 10
- 535
- 0
Báo cáo khoa học: Contribution of a central proline in model amphipathic a-helical peptides to self-association, interaction with phospholipids, and antimicrobial mode of action ppt
Ngày tải lên :
23/03/2014, 10:21
... Sugihara G (1996) Design and synthesis of amphiphilic alpha-helical model peptides with systematically varied hydrophobic-hydrophilic balance and their interaction with lipid- and bio-membranes Biochemistry ... initial electrostatic interactions, whereas the interaction of PFPs with membranes was most affected by hydrophobic interactions These properties may lead to the selectivity of PCPs and ⁄ or the nonselectivity ... fluorescence intensity achieved by the peptides, and F0 and Ft are the initial fluorescence intensities observed without the peptides and after treatment with Triton X-100, respectively All conditions...
- 15
- 376
- 0
Báo cáo khoa học: Aptamers toEscherichia colicore RNA polymerase that sense its interaction with rifampicin, r-subunit and GreB ppt
Ngày tải lên :
23/03/2014, 13:20
... in the interaction with RNA and DNA in the elongation complex At elevated ionic strength, Rif and r70 suppressed RNAP–aptamer interactions (above) Under low ionic strength conditions, Rif and r70 ... reports on interactions of the core with total cellular DNA and RNA [43,46], tRNA [47,48], ssDNA [43] and also some individual RNA sequences [49] Reported Kd values for some of these interactions ... RNAP with E4 and E10 aptamers, it caused their rapid dissociation; half-life times of the complexes were reduced by more than 10 times (5 in comparison with > h when the kinetics was measured without...
- 11
- 303
- 0
Báo cáo khoa học: The enzymatic activity of SR protein kinases 1 and 1a is negatively affected by interaction with scaffold attachment factors B1 and 2 pot
Ngày tải lên :
30/03/2014, 01:20
... them with GST–SAFB1C, GST–SAFB1CDRE, GSTSAFB2C and GST bacterial preparations The beads were washed and the eluted proteins were probed with the anti-GST IgG for SAFB protein detection and the ... These results confirm the direct interaction of SAFBs with the SRPK1 ⁄ 1a proteins and provide a direct link between the affinity of the SAFB–SRPK1 ⁄ 1a interaction and the extent of the inhibition ... substrate with the catalytic region of the kinase, on the one hand, and of the positive charge of the RS domain with the negatively charged ‘docking groove’ of SRPK1, on the other hand The RE-rich...
- 16
- 573
- 0
Báo cáo khoa học: Cholesterol interaction with the related steroidogenic acute regulatory lipid-transfer (START) domains of StAR (STARD1) and MLN64 (STARD3) ppt
Ngày tải lên :
30/03/2014, 04:20
... STARD1START is well suited for a ligand with the size and shape of cholesterol [29,30] Critical residues proposed to interact with cholesterol are localized within the fragment containing amino ... yellow, and comprises half of the a3-helix and the strands b3–b7, including their connecting loops The residues Glu107 (E), Arg126 (R) and Leu137 (L) (all marked in magenta) are located within ... region and have been proposed to interact with cholesterol (see Discussion) Otherwise, the model is colored according to the secondary structure, with helices in red, b-strands in green, and loops...
- 13
- 404
- 0
Báo cáo khoa học: Interaction with model membranes and pore formation by human stefin B – studying the native and prefibrillar states docx
Ngày tải lên :
30/03/2014, 04:20
... isoform and, again, this was stronger that the wild-type protein The dissociation of the G4R and StB-wt was fast and almost complete within min, whereas the dissociation of StB-Y31 was slower and ... the stable pores They are less stable (levels and in Fig 4A) and are characterized by fast opening and closing Moreover, the pore formation process and the presence of different conductance levels ... (+120 mV) and negative voltage ()120, )100 and )80 mV) The traces are representative of three independent experiments 120 –120 suggests a strong interaction with the membrane in accordance with the...
- 12
- 315
- 0
jhttransport phenomena and droplet formation during pulsed laser interaction with thin films
Ngày tải lên :
06/05/2014, 08:55
... incident laser pulse energies of 2.0 and 2.7 J with 20 nsec pulse width ͑FWHM͒ and a radius of 9.5 m incident on a 0.3 m chromium film A domain with a radius of 12 m and axial depth of 0.9 m is used ... simulations are performed with and without the recoil pressure term in Eq ͑7͒ for both 2.0 and 2.7-J laser pulses Due to the time required to perform a simulation with a temperature-dependent ... Nd:YLF laser and exposes the film at the desired time A 35-mm camera captures the illumination of the dye laser and stops the motion of the fluid on film A 600-nm filter with a 40-nm bandwidth is...
- 8
- 228
- 0
more straw bale building a complete guide to designing and building with straw - chris magwood
Ngày tải lên :
04/06/2014, 13:11
... movement has grown and evolved and so has this book In this updated and expanded second edition, the authors provide the latest understandings and techniques to build smarter, better, and more simply ... Manitoba Times — and everything in the straw bale world — are changing, and so are our building methods .With More Straw Bale Building, the authors have taken an old standard and brought it up ... al Cam Todd and Canadian Classic Contractors Catherine Wanek and Pete Fust Cari and Russ Cheryl, Beth and Grace Chris and Judith Plant Chris Walker Dale Brownson Dave McKey, Goldie and others...
- 297
- 505
- 0
Báo cáo sinh học: " Phosphorylation of HIV Tat by PKR increases interaction with TAR RNA and enhances transcription" potx
Ngày tải lên :
18/06/2014, 22:20
... (lanes and 8), 50 mM (lanes and 9), 100 mM (lanes and 10), 200 mM (lanes and 11), 500 mM (lanes and 12), and 1000 mM (lanes and 13) Lanes 2–7 show the dissociation of the Tat-N-TAR complex, and ... expanding, carry mutations in Tat R57S and G63Q within and close to the basic domain, and yet exhibit increased transcriptional activity [40] On the other hand, the phosphorylation of serines and ... was washed and the HIStagged Tat proteins were eluted with M guanidine-HCl, pH 4.0 The fractions collected were dialysed in 0.1 mM DTT and then analysed by 15% SDS-PAGE and stained with Coomassie...
- 13
- 410
- 0