absolute quantification of toxicological biomarkers by multiple reaction monitoring
Quantification of Microcystin-degrading Bacteria in a Biofilm from a Practical Biological Treatment Facility by Real-time PCR
... respectively Quantification of microcystin-degrading bacteria by real-time PCR The standard curve was generated from serial 10-fold dilutions of DNA carrying the mlrA of strain C-1 The quantification of ... amplification reactions required µL of extracted DNA, 10 pmol of each primer, 30 pmol of TaqMan probe, 25 µL of TaqMan Gene Expression Master Mix (Applied Biosystems Co USA), made up to a final volume of ... reference** Rapala et al., 2005 Isolated by our research group Provided by Dr Fujimoto from Tokyo University of Agriculture ** Biofilm samples Samples of biofilm (10 g) were taken from a biological...
- 9
- 522
- 0
Báo cáo khoa học: Reduction of S-nitrosoglutathione by human alcohol dehydrogenase 3 is an irreversible reaction as analysed by electrospray mass spectrometry pptx
... determine the structure of the product generated by ADH3 catalysed reduction of GSNO, nano-electrospray mass spectra of the reaction mixture with and without the addition of enzyme were recorded ... activity of the human form of ADH3 for this compound Recombinant expression and purification of wild-type and mutated forms of the enzyme enabled kinetic characterization and investigation of substrate–enzyme ... reduction of GSNO catalysed by ADH3 is clearly an irreversible reaction With these observations taken into account, the reaction may therefore be forced in the reductive direction thereby ÔoverridingÕ...
- 8
- 290
- 0
Báo cáo nghiên cứu khoa học: "COMPUTE AND DEFINE EXACTLY THE REGION OF ELASTIC REACTION FORCE FOR CALCULATING THE SECTION FORCE OF UNDERGROUND CONSTRUCTION BY FINITE ELEMENT METHOD" pptx
... the research of M.M.Protodiakonov, the vertical pressure of soil is affected to the tunnel structure caused by the weigh of mass stratum, which were undermined limit by the pressure of tunnel arch ... TẬP 11, SỐ 05 - 2008 6.RESULT OF THE CALCULATION OF SECTION FORCE AND DEFINE THE REGION OF ELASTIC REACTION FORCE OF UNDERGROUND STRUCTURE 6.1.The receiving result of mesh 40 element : 30 elements ... convergency of problem while define the region of elastic reaction force a) In order to make this comparison of the interdependent of angle ưo, we consider and survey the changing cases of tunnel...
- 10
- 328
- 0
Báo cáo y học: "dentification of blood biomarkers of rheumatoid arthritis by transcript profiling of peripheral blood mononuclear cells from the rat collagen-induced arthritis model" doc
... development of CIA may suggest potential novel biomarkers for RA This could be of value both in monitoring the effects of drugs on disease progression and in discovering potential biomarkers, ... development of novel treatments for RA In the present study we described a noninvasive strategy to discover RA biomarkers by transcript profiling of peripheral circulating lymphocytes As an initial proof -of- concept, ... advance our understanding of and facilitate identification of novel biomarkers for RA Conclusion We established a noninvasive strategy to identify biomarkers by gene expression profiling in PBMCs in...
- 14
- 423
- 0
báo cáo khoa học: "Quantification of specific bindings of biomolecules by magnetorelaxometry" docx
... of human serum (Figure 6) But the relaxation time is by a factor of 1.4 smaller than that of the serum sample This difference is related to the viscosity of serum of about mPa s being twice of ... We diluted the stock suspension of biotin-agarose by a factor of with PBS The MNP*SAV were diluted with PBS by factors of and 10; MNP*AB were diluted by factors of 10 and 100 For the MARIA-measurement ... the signal of the transducers changes as the result of the binding of the detection molecule, i.e the amount of bound detection molecules can be measured in the presence of unbound ones by separating...
- 12
- 126
- 0
Báo cáo khoa hoc:" Quantitative competitive reverse transcription polymerase chain reaction is not a useful method for quantification of CD4 and CD8 cell status during HIV infection" pptx
... the concentration of unknown amount of DNA – that of CD4, CD8 or GAPDH The success of the RNA extraction and reverse transcription is determined by quantification of GAPDH mRNA by QC-RT-PCR Each ... µl PCR The remainder of the reactions were made up by µl PCR Buffer (Gibco BRL, Grand Island, NY), 0.25 µg of each primer, mM MgC12 in each CD4 reaction and mM MgC12 for CD8 reactions, mM total ... intensity (as determined by analysis of PICT files with NIH image) on the x-axis versus the log of incremental dilutions of competitor DNA on the y-axis Solving for the antilog of x when y = will give...
- 4
- 319
- 0
Báo cáo sinh học: "Estimation of genetic parameters of egg production traits of laying hens by restricted maximum likelihood applied to a multiple-trait reduced animal model" pps
... at each iteration, of direct inversion of a matrix of size equal to the number of parents This burden was reduced by tridiagonalizing the coefficient matrix through a series of orthogonal transformations, ... purposes of this study were: 1) to estimate genetic parameters of correlated egg production traits by REML applied to a multiple- trait reduced animal model; and 2) to show the application of some ... multiplication of the d right hand side by L’ (Smith and Graser, 1986) The solutions for ap and a are e = of the form: As shown in !9!, this method led to the tridiagonalization of a complex matrix of size...
- 14
- 227
- 0
Quantification of primary and secondary oocyte production in atlantic cod by simple oocyte packing density theory
... measurement of sectioned oocytes Measurement of the ovarian volume fraction of these oocytes by using Delesse’s (1847) principle Calculation of oocyte numbers from simple packing theory of spheres ... LVOs) started off with an early production of CAOs already around 180 μm, followed successively by the production of EVOs and LVOs but then leveling off (Figure 9) There were indications of a decline ... Metcalfe 2006 A field and experimental evaluation of the effect of data storage tags on the growth of cod Journal of Fish Biology 68:385–400 QUANTIFICATION OF OOCYTE PRODUCTION Rollefsen, G 1934 The...
- 15
- 593
- 0
Quantification of epidermal growth factor receptor dynamics and interactions in living cells by fluorescent correlation and cross correlation spectroscopy
... sequence of PMT 64 2.2 The map of the vectors 64 PTB 65 3.1 The PTB sequence 65 3.2 Map of the vector of the plasmid 66 Map of mRFP-EGFR-EGFP ... 121 7.2.1 The heterogeneity of EGFR diffusion revealed by ITIR-FCS diffusion law 121 7.2.2 The effect of cholesterol depletion by mβCD on the mobility of EGFR 122 7.2.3 Cytoskeletal effects ... coefficients of Dil analogues 109 Table Summary of diffusion coefficients 134 Table Summary of intercepts 135 x List of Figures Figure 1 The Crystal Structure of the 2:2...
- 186
- 1,434
- 0
Quantification of spheroid formation mechanism by size and shape analysis
... TABLE OF CONTENTS TABLE OF CONTENTS Page ACKNOWLEDGEMENTS i TABLE OF CONTENTS .ii SUMMARY vi LIST OF TABLES ix LIST OF FIGURES xi LIST OF ... Drying of spheroids .35 2.7 Characterization of spheroids .35 2.7.1 Size analysis by sieving .35 2.7.2 Sizing by image analysis 36 2.7.3 Roundness quantification by ... fitting of size distribution of RT(SR) spheroids to statistical distributions 114 Table 21 Size distribution of RT(SR) spheroids as defined by 3cMG distribution 118 x LIST OF FIGURES LIST OF...
- 179
- 226
- 0
Quantification of biomolecule dynamics and interactions in living zebrafish embryos by fluorescence correlation spectroscopy
... support, love and the happiest moments she brings to my life. II Table of Contents Acknowledgements II Table of Contents III Summary VI List of Tables VIII List of Figures IX List of Symbols and acronyms ... A correlation between two variables a and b describes the dependence of these two variables. In practical terms this means that if we know the value of a or b we can make some prediction of the value of b or a, respectively. The concept of ... photophysical dynamics of fluorophores, diffusion coefficients of DNAs and proteins, and dissociation constants of interacting particles. In this work, we extended the application of FCS and ...
- 158
- 305
- 0
Dynamics Of Anisotropic Composite Cantilevers Weakened By Multiple Transverse Open Cracks
... structural health monitoring The present research addresses the problem of vibration of prismatic beams of length L, width b and height h, weakened by a sequence of surface open cracks, of uniform but ... behavior of beams weakened by two and multiple transverse surface open cracks was investigated), the specialized literature is quite void of research works addressing the problem of free vibration of ... free vibration characteristics of anisotropic beams weakened by a sequence of surface open cracks was presented Results that reflect the implications of multiple cracks of equal or different depths...
- 19
- 192
- 0
Báo cáo y học: " the Value of Serum Biomarkers (Bc1, Bc2, Bc3) in the Diagnosis of Early Breast Cancer"
... biomarkers by surface-enhanced laser desorption/ionization time -of- flight mass spectrometry (SELDI-TOF MS) They observed three serum peaks to distinguish BC patients from controls by SELDI-TOF ... essential to discover new biomarkers to manage different stages of BC development Biomarkers may be promising in diagnosing development or progress of the disease and monitoring the treatment ... 23–71; mean age 39.1) Complex protein profiles of sera of 27 women with BC, 24 women with benign breast disease and 37 healthy women were obtained by SELDI-TOF MS analyses using IMAC30-NI beams...
- 8
- 584
- 0
A STUDY ON SOLUBILIZATION OF EXCESS SLUDGE BY HIGH SPEED ROTARY DISK PROCESS
... production of BOD5 by hydrolase and the consumption of BOD5 by active biomass Therefore, the rapid increase of BOD5 during the initial period was likely due to hydrolyzation of fragment of cell by enzyme ... treatment of high concentration of sludge; 2) investigation of optimal operating condition; 3) extent of improvement of lower molecular through hydrolysis; 4) estimation of the existence of biomass; ... definition of “solubilization” If biodegradability of samples is used as explanation of solubilization, the definition of this basic standard should be necessary From the view of the change of the...
- 6
- 377
- 0
Improvement of Water Quality by Removal of Suspended Solids and High-speed Biological Filtration in Closed Water Body
... influence of these disturbances was found Some sampling points were selected They are the outlet of plant, the outside of experimental area, points of horizontal level and 0.5, 1.0, 1.5 m of vertical ... outlet of equipment Transparency[cm] 60 out of experimental area 50 40 30 20 10 10 15 20 25 30 35 40 45 50 Time[d] Figure Variation of Transparency Variation of COD concentration Variation of COD ... system, and the removal of nitrogen should be considered, too References [1] Department of Water Environment, Ministry of the Environment, Japan July 2002 Outline of Administration of Water Environment...
- 6
- 526
- 0
Treatment of Textile Wastewater by a Coupling of Activated Sludge Process with Membrane Separation
... minutes Fig Effect of backflush pressure on the flux, MLSS of 8700 mg/l, TMP of 0.4 bar, CFV of 0.88 m/s, backflushing pressure of 1.5 bar and backflushing interval of s Fig Effect of backflush interval ... MINUTES Fig Effect of TMP on the membrane flux, MLSS of 9300 mg/L, CVF (v) of 0.79 m/s, without backflush Fig Effect of CFV (v) on the membrane flux, MLSS of 9300 mg/L, TMP of 0.4 bar, without ... Effect of backflush interval on the flux, MLSS of 8700 mg/l, TMP of 0.4 bar, CFV of 0.88 m/s, backflushing pressure of 1.5 bar and backflushing interval of s Main experiments In the main experiments,...
- 8
- 434
- 0
Comparative decolorizing efficiency of textile dye by mesophilic and thermophilic anaerobic treatments
... efficiency by digested sludge The potential of digested sludge on MO and RB4 tested by variation of dye concentration was shown by the different extent of decolorizing efficiency of the tested ... carbon (TOC) The reduced form of dyes after being treated by digested sludge was studied by monitoring the change of spectrum wavelength between 300-650 nm The appearance of a new peak would be presented ... concentration of RB4 was 100 mg·L-1 (Figure 8), which confirmed the acceleration by an increase of treatment temperature Reducing byproducts of RB4 did not show any auto-oxidizing reaction, and...
- 10
- 405
- 0
Present Status and Feature of Groundwater Contamination by Nitrate-nitrogen in Kumamoto City
... Plateau 30 monitoring monitoring monitoring monitoring monitoring monitoring monitoring general use 31.7~42.7 monitoring 121.5~138.0 monitoring general use 71.9~93.7 monitoring 113.0~129.5 monitoring ... 71.4~93.5 monitoring general use unknown monitoring 120.5~131.5 monitoring 48.0~59.0 monitoring general use 39.0~50.0 monitoring general use 22.3~33.3 monitoring 97.0~108 monitoring 82.3~98.8 monitoring ... monitoring 113.0~129.5 monitoring 6.8~12.3 monitoring 127.5~149.5 monitoring 4.5~10 monitoring 112~145 monitoring general use general use 71.5~82.5 monitoring 46 155 24 25 shallow deep 24.7~35.7...
- 10
- 498
- 0
Control of Membrane Fouling by Coagulant and Coagulant Aid Addition in Membrane Bioreactor Systems
... substances (EPS) of the mixed liquor were extracted by steam treatment at 105 oC for 30 minutes (Nakajima and Mishima, 2005) For the SMP
- 11
- 575
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25