abiotrophia granulicatella species by 16s rrna based pcr and rflp
Báo cáo y học: "Identification of bacteria on the surface of clinically infected and non-infected prosthetic hip joints removed during revision arthroplasties by 16S rRNA gene sequencing and by microbiological culture" doc
Ngày tải lên :
09/08/2014, 10:20
... a 2% agarose gel, and amplified DNA was detected by staining with ethidium bromide (0.5 μg/ml) and examination under ultraviolet illumination Cloning of 16S rRNA PCR products PCR products were ... gene products amplified by PCR for each sample, 50 clones from each generated library were randomly selected The 16S rRNA gene insert from each clone was amplified by PCR with the use of the ... Bacterial species identified by 16S rRNA gene sequencing of isolates from 10 prosthetic hip joints Species Table shows the isolates obtained from the 10 prosthetic hip joints by culture and identified...
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Báo cáo khoa học: "Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes" ppt
Ngày tải lên :
12/08/2014, 04:21
... symptoms developed) were tested for PVM by ELISA and RT -PCR All progeny tubers were tested by RT -PCR followed by RFLP analysis for confirmation ELISA Potato tubers and leaves as well as indicator plants ... enhanced by using composite leaf and tuber samples Restriction fragment length polymorphism (RFLP) was introduced to verify PCR amplicon identity Results Initial tests by ELISA and RT -PCR All ... of PCR products by restriction analysis PCR amplicons (520 bp) were produced in RT -PCR using primers PVM3 and PVM4 from RNA extracted from PVM-Ca508 infected tuber (lanes 2), leaf (lane 4) and...
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Báo cáo khoa học: " Identification and prevalence of Ehrlichia chaffeensis infection in Haemaphysalis longicornis ticks from Korea by PCR, sequencing and phylogenetic analysis based on 16S rRNA gene" docx
Ngày tải lên :
07/08/2014, 18:21
... chaffeensis-specific 16S rRNA gene fragment For the first round PCR, primer ECC (5'-agaacgaacgctggc ggcaagc-3') and ECB (5'-cgtattaccgcggctgctggca-3') targeting the conserved regions of Ehrlichia spp 16S rRNA ... sec, and one cycle of extension at 72 C for For second round nested -PCR, pmol of each primer, HE1 and HE3 (Genotech, Korea) and µl of first PCR product as template DNA were included in the PCR ... vegetation and various animals from nine provinces of Korea were subjected to screening by genus-specific (Ehrlichia spp or Anaplasma spp.) and species- specific (E chaffeensis) PCR based on amplification...
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Báo cáo y học: "Broad-range PCR, cloning and sequencing of the full 16S rRNA gene for detection of bacterial DNA in synovial fluid samples of Tunisian" pps
Ngày tải lên :
09/08/2014, 14:22
... antibodies by Micro Immunofluorescence assay as described by Wang and Grayston [29] bSerology was determined for Ct-IgA antibodies by ELISA (Labsystems, Hilsinki, Finland) cChlamydia PCR in genital ... -80°C until analysis Automated DNA extraction from synovial fluid and broad-range PCR amplification of 16S rRNA genes, cloning and sequencing of bacterial DNA For 10 minutes, 500 μl of SF were ... and 1% SDS) and 25 μl of proteinase K (10 mg/ml) (Sigma, St Louis, MO, USA) were added to the SF cell pellet Bacterial 16S rDNA fragments were amplified from SF extracted DNA by broad-range PCR...
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Nhân đoạn gen mã hóa 16s rRNA và trình tự ITS2 của các loài ốc cối conus bằng kỹ thuật PCR
Ngày tải lên :
14/08/2014, 18:20
... gen mã hóa 16S rRNA (hệ gen ty thể) (Espiritu cs, 2001) đoạn chèn ITS2 (Internal Transcribed Spacer 2) (hệ gen nhân) (Nam cs, 2009) 26 a) Nhân đoạn gen mã hóa16S rRNA Cặp mồi 16S: 16SF-5′-CCG ... 3.2 Nhân đoạn gen mã hóa 16S rRNA trình tự ITS2 mẫu ốc cối kỹ thuật PCR Đoạn gen mã hóa 16S rRNA đoạn chèn ITS2 loài ốc cối khuếch đại sử dụng hai cặp mồi tham khảo 16S (Espiritu cs, 2001) ITS2 ... cytochrome b Các gen khác bao gồm gen mã hóa rRNA (gen rrnS mã hóa 12S rRNA gen rrnL mã hóa cho 16S rRNA) 22 gen mã hóa tRNA Hai tiểu đơn vị lớn nhỏ rRNA 17 chép từ sợi tRNA cho M, Y, C, W, H,...
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Ứng dụng khóa phân loại hình thái và vùng 16S rRNA trên DNA ty thể trong định danh cá bột thuộc họ
Ngày tải lên :
06/11/2012, 09:49
... pháp PCR khuếch đại vùng 16S mtDNA Vùng trình tự gen mã hóa 16S rRNA đƣợc khuếch đại từ mtDNA theo phƣơng pháp PCR (Na-Nakorn cộng sự, 2006) Cụ thể nhƣ sau: phản ứng PCR (50 µl) gồm: 25 µl PCR ... phản ứng PCR nhân dòng vùng gen mã hóa 16S rRNA mtDNA Sản phẩm PCR đƣợc phân tích gel agarose 1% (Hình 4.5) 37 M M 831 bp 630 bp 947 bp 564 bp Hình 4.5.: Sản phẩm PCR khuếch đại vùng 16S rRNA mtDNA ... vùng 16S rRNA mtDNA mẫu cá P macronema .39 Hình 4.7: Sự tƣơng đồng trình tự vùng 16S rRNA mtDNA mẫu cá P hypophthalmus 41 Hình 4.8: Sự tƣơng đồng trình tự vùng 16S rRNA...
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Ứng dụng khóa phân loại hình thái và vùng 16s rRNA trên DNA ty thể trong định danh cá bột thuộc họ Pangasiidae
Ngày tải lên :
17/11/2012, 09:46
... pháp PCR khuếch đại vùng 16S mtDNA Vùng trình tự gen mã hóa 16S rRNA đƣợc khuếch đại từ mtDNA theo phƣơng pháp PCR (Na-Nakorn cộng sự, 2006) Cụ thể nhƣ sau: phản ứng PCR (50 µl) gồm: 25 µl PCR ... phản ứng PCR nhân dòng vùng gen mã hóa 16S rRNA mtDNA Sản phẩm PCR đƣợc phân tích gel agarose 1% (Hình 4.5) 37 M M 831 bp 630 bp 947 bp 564 bp Hình 4.5.: Sản phẩm PCR khuếch đại vùng 16S rRNA mtDNA ... vùng 16S rRNA mtDNA mẫu cá P macronema .39 Hình 4.7: Sự tƣơng đồng trình tự vùng 16S rRNA mtDNA mẫu cá P hypophthalmus 41 Hình 4.8: Sự tƣơng đồng trình tự vùng 16S rRNA...
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Ứng dụng khóa phân loại hình thái và vùng 16s rRNA trên DNA ty thể
Ngày tải lên :
19/11/2012, 15:17
... pháp PCR khuếch đại vùng 16S mtDNA Vùng trình tự gen mã hóa 16S rRNA đƣợc khuếch đại từ mtDNA theo phƣơng pháp PCR (Na-Nakorn cộng sự, 2006) Cụ thể nhƣ sau: phản ứng PCR (50 µl) gồm: 25 µl PCR ... phản ứng PCR nhân dòng vùng gen mã hóa 16S rRNA mtDNA Sản phẩm PCR đƣợc phân tích gel agarose 1% (Hình 4.5) 37 M M 831 bp 630 bp 947 bp 564 bp Hình 4.5.: Sản phẩm PCR khuếch đại vùng 16S rRNA mtDNA ... vùng 16S rRNA mtDNA mẫu cá P macronema .39 Hình 4.7: Sự tƣơng đồng trình tự vùng 16S rRNA mtDNA mẫu cá P hypophthalmus 41 Hình 4.8: Sự tƣơng đồng trình tự vùng 16S rRNA...
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ỨNG DỤNG KHÓA PHÂN LOẠI HÌNH THÁI VÀ VÙNG 16S rRNA TRÊN DNA TY THỂ TRONG ĐỊNH DANH CÁ BỘT THUỘC HỌ Pangasiidae
Ngày tải lên :
03/09/2013, 10:00
... pháp PCR khuếch đại vùng 16S mtDNA Vùng trình tự gen mã hóa 16S rRNA đƣợc khuếch đại từ mtDNA theo phƣơng pháp PCR (Na-Nakorn cộng sự, 2006) Cụ thể nhƣ sau: phản ứng PCR (50 µl) gồm: 25 µl PCR ... phản ứng PCR nhân dòng vùng gen mã hóa 16S rRNA mtDNA Sản phẩm PCR đƣợc phân tích gel agarose 1% (Hình 4.5) 37 M M 831 bp 630 bp 947 bp 564 bp Hình 4.5.: Sản phẩm PCR khuếch đại vùng 16S rRNA mtDNA ... vùng 16S rRNA mtDNA mẫu cá P macronema .39 Hình 4.7: Sự tƣơng đồng trình tự vùng 16S rRNA mtDNA mẫu cá P hypophthalmus 41 Hình 4.8: Sự tƣơng đồng trình tự vùng 16S rRNA...
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Báo cáo khoa học: The Y42H mutation in medium-chain acyl-CoA dehydrogenase, which is prevalent in babies identified by MS/MS-based newborn screening, is temperature sensitiv pptx
Ngày tải lên :
07/03/2014, 16:20
... for the Y42H and K304E mutations, identified by newborn screening, and who are followed by the authors (SEM and DMF), have been admitted to the hospital with significant lethargy and vomiting during ... of monomeric and tetrameric MCAD proteins were measured at 26 °C and 41 °C as described previously [25] Briefly, soluble and insoluble MCAD proteins were separated by centrifugation and the respective ... genotypes K304E/K304E, K304E/Y42H, K304E/WT and WT/WT cultured at 34 °C, 37 °C and 39 °C The tetrameric and the soluble MCAD protein was measured by native (A) and denaturing (B) PAGE in combination...
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Báo cáo khoa học: "Text Chunking by Combining Hand-Crafted Rules and Memory-Based Learning" pot
Ngày tải lên :
17/03/2014, 06:20
... chunking Korean by combining the handcrafted rules and a memory -based learning Our method is based on the rules, and the estimates on chunks by the rules are veried by a memory -based learning ... supported by the Korean Ministry of Education under the BK21-IT program and by the Korean Ministry of Science and Technology under NRL and BrainTech programs References V Cherkassky and F Mulier ... is adopted for memory -based learning Decision trees and SVMs use the same attributes with memory -based learning (see Table 2) Two of the algorithms, memory -based learning and decision tree, show...
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BÁO CÁO " VỊ TRÍ PHÂN LOẠI CỦA CÁC CHỦNG CLOSTRIDIUM PERFRINGENS PHÂN LẬP TẠI VIỆT NAM DỰA VÀO TRÌNH TỰ GEN 16S rRNA " doc
Ngày tải lên :
25/03/2014, 04:21
... đá phút, bảo quản DNA 20oC Đối với gen mã hóa 16S ribosome RNA, sử dụng cặp mồi nhân đoạn gen 714bp 16S rRNA: 16S- F1 (5’-CGAGCGATGAAGTTTCCTT-3’) 16S- R1 (5’ACAGTCCAGAGAGTCGCCTT-3’) - Giải trình ... al., (2002) Phylogentic analysis and PCR detection of Clostridium chauvoei, Clostridium haemolyticum, Clostridium novyi type A and B, and Clostridium septicum based on the flagellin gene Vet Microbiol ... khuẩn giải mã GenBank ̀ ngân hàng liệu lớn lưu giữ 20 triệu chuỗi gen, 90.000 gen 16s rRNA So sánh trình tự gen 16S rRNA 100 loài Clostridium, Collins cs (1994) [Error! Reference source not found.];...
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Báo cáo khoa học: Monitoring the prevention of amyloid fibril formation by a-crystallin Temperature dependence and the nature of the aggregating species pdf
Ngày tải lên :
30/03/2014, 04:20
... increased by and the density decreased 0.02 ngÆmm)2 by 0.0072 gÆcm)3 After 2.5 h, the thickness increased by 0.212 nm, the mass increased by 0.05 ngÆmm)2 and the density decreased by 0.0167 gÆcm)3, ... of the experiment By the end of the measurement, the thickness had increased by 0.342 nm, the mass had increased by 0.10 ngÆmm)2 and the density had decreased by 0.024 gÆcm)3 By contrast, on channel ... to suppress fibril formation, as shown by TFT binding data, was supported by TEM At 37 and 60 °C, a-synuclein, by itself, formed fibrils of comparable length and morphology, however, in the presence...
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Báo cáo khoa học: Nop53p interacts with 5.8S rRNA co-transcriptionally, and regulates processing of pre-rRNA by the exosome ppt
Ngày tải lên :
30/03/2014, 04:20
... to pre -rRNA Nop53p is a nucleolar protein that has previously been shown to be involved in pre -rRNA processing and to co-immunoprecipitate the 27S and 7S pre-rRNAs and the mature 5.8S rRNA [31–33], ... study study Maintenance and handling of E coli and yeast strain Escherichia coli strains DH5a and BL21(DE3) were maintained in LB medium and manipulated according to standard techniques [47] The ... late steps of pre -rRNA processing, and is directly responsible for the 3¢ fi 5¢ exonucleolytic digestion of the 3¢ extension of the 7S pre -rRNA and formation of the mature 5.8S rRNA [13] Interestingly,...
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Báo cáo khoa học: Characterization of the 16S rRNA- and membrane-binding domains of Streptococcus pneumoniae Era GTPase Structural and functional implications docx
Ngày tải lên :
31/03/2014, 07:20
... resulted in the loss of 16S rRNA- binding activity [20] Thus, the KH domain is required for 16S rRNA- binding activity However, it is not clear whether the binding of Era to 16S rRNA requires the GTPase ... coli and Mycoplasma pneumoniae and compared their 16S rRNA- binding activity with that of the full-length Era proteins The C-terminal parts of both Era proteins were able to bind to 16S rRNA in ... binding of the protein to 16S rRNA, and part of the domain is also required for the binding of the protein to membrane Thus, the regions critical for the membrane- and 16S rRNA- binding activities...
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Báo cáo Y học: Shifted positioning of the anticodon nucleotide residues of amber suppressor tRNA species by Escherichia coli arginyl-tRNA synthetase pdf
Ngày tải lên :
31/03/2014, 23:20
... Randomization of genes by PCR mutagenesis PCR Methods & Applications 2, 28 –33 13 Sissler, M., Giege, R & Florentz, C (1996) Arginine aminoacylation identity is context-dependent and ensured by ... activity, but not the lysine- and tyrosine-accepting activities, of the tRNA was increased, and (b) the recognitions of the tRNA by LysRS and TyrRS were more impaired than that by ArgRS The present results ... specificity by decreasing the recognition by LysRS and TyrRS much more than that by ArgRS Genetic selection of mutant ArgRS molecules To explore the accommodation of the suppressor tRNA by ArgRS,...
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báo cáo hóa học:" Whole blood assessment of antigen specific cellular immune response by real time quantitative PCR: a versatile monitoring and discovery tool" potx
Ngày tải lên :
18/06/2014, 15:20
... extracted, reverse transcribed and amplified by qRT -PCR in the presence of primers and probes specific for the indicated genes and β-actin house keeping gene (panels A-F) Cytokine and chemokine gene expression ... first-strand buffer, μl 0.1 M DTT and μl (200 units) M-MLV reverse transcriptase (all by Invitrogen Ltd., Paisley, UK) and incubated at 37°C for hour Two μl of cDNA were used for each PCR amplification ... inadequate, if at all available In this work our aim was to design and test a RT -PCR based technique easily amenable to standardization and automation for the monitoring of cellular immune responses...
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báo cáo hóa học:" Heterogeneous activation of the TGFβ pathway in glioblastomas identified by gene expression-based classification using TGFβ-responsive genes" pptx
Ngày tải lên :
18/06/2014, 15:20
... tissues and species, core TGFβ-responsive genes were identified in multiple studies showing the independence of tissue and species origins 445 (17%) genes were identified in independent studies and ... decapentaplegic" (Mad) and the C elegans gene Sma SMAD2 and SMAD3 specifically mediate the signals induced by TGFβ Phosphorylated SMAD2/3 are released from the receptor complex and bind to SMAD4 The ... given tissues and the transcription profiles upon TGFβ stimulation would be quite diversified in different tissues and species [17,19-24] The transcriptional responses generated by chronic TGFβ...
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báo cáo hóa học:" Airborne particulate matter PM2.5 from Mexico City affects the generation of reactive oxygen species by blood neutrophils from asthmatics: an in vitro approach" docx
Ngày tải lên :
20/06/2014, 00:20
... and Toxicology 2009, 4:17 Figure by neutrophils in contact with PM2.5 In vitro generation of reactive oxygen and nitrogen species In vitro generation of reactive oxygen and nitrogen species by ... diverse forms including spheres (1, and 8), clusters (2, and 7), plates (5 and 6) and reticular forms (9) corresponding to PM10 particles (indicated by numbers 1–5) and the fine fraction (6–9), (Figure ... was found in all categories and accounted for less than 3% and 1.5% in the coarse and fine fractions, respectively The presence of Fe and Cu content into spherical and soot aggregates of the fine...
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