aberrant glycosphingolipid expression and membrane organization in tumor cells consequences on tumor host interactions
Tài liệu Báo cáo khoa học: Modulation of oat arginine decarboxylase gene expression and genome organization in transgenic Trypanosoma cruzi epimastigotes docx
Ngày tải lên :
19/02/2014, 07:20
... foreign gene expression and to investigate whether this modulation can be explained by the concomitant changes occurring in the parasite genome organization Results and Discussion Expression of the ... Samples containing 20 lg of total RNA were fractionated by electrophoresis on a 1% agarose gel, containing 2.2 m formaldehyde, and blotted onto nylon membranes Southern and northern hybridization assays ... between and weeks after transfection However, the continuous selection pressure of the antibiotic, G418, allowed stable expression of the ADC gene, presumably after recombination and integration into...
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báo cáo khoa học: " Expression of Ets-1, Ang-2 and maspin in ovarian cancer and their role in tumor angiogenesis" pps
Ngày tải lên :
10/08/2014, 10:21
... papillary cystadenocarcinoma; D: Ang-2 expression in ovarian borderline mucinous cystadenoma; E: Maspin expression in mucinous cystadenocarcinoma; F: Maspin expression in mucinous cystadenoma The ... similar expression pattern in malignant and benign tumors (Figure 1C, D) Maspin expression was predominantly located in the cytoplasma and occasionally in the nucleus of epithelium and cancer cells ... underlying the localization of maspin and its interaction with Ets-1 warrant further investigations In this study we employed IHC to evaluate the expression of Ets-1, Ang-2 and maspin in clinical...
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Báo cáo khoa học: Lithium increases PGC-1a expression and mitochondrial biogenesis in primary bovine aortic endothelial cells ppt
Ngày tải lên :
23/03/2014, 09:20
... within cells and tissues reflect changes in energy demand and hormonal status, and depend mainly on changes in the activity and ⁄ or expression of the transcription factors NRF1 and NRF2 and coactivator ... [30] Inversely, increasing the expression of PGC-1a was shown to be neuroprotective in the mutated-hungtintin transgenic mouse model of Huntington disease [30] Interestingly, the preconditioning ... during preconditioning in heart and brain ischemic–reperfusion injury models [8,9], and in neurodegenerative disease models [6], have been studied only in relation to the apoptotic function of...
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Báo cáo khoa hoc:" Adaptive significance of amylase polymorphism in Drosophila. Analysis of the association between tissue-specific expression and specific activity in Amy or Amy genotypes F S of Drosophila subobscura" ppt
Ngày tải lên :
09/08/2014, 18:21
... locus and the variability of tissue-specific expression [9] The latter type of variability is represented by the number and position of the amylase activity regions in the midgut [5] Inter- and intrapopulation ... correlation on all categories, indicate statistically non-significant negative correlations in five cases Such correlations are found mostly in the groups in which the number of active regions or ... A!nyF (F) alleles, inbred for 20 generations in optimal laboratory conditions en masse, were taken for dissection of the midgut and for the specific enzyme activity assay Determination of the specific...
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Báo cáo y học: "Downregulation of protein disulfide isomerase in sepsis and its role in tumor necrosis factor-alpha release" pps
Ngày tải lên :
13/08/2014, 11:22
... isomerase inhibition on tumor necrosis factor-alpha gene expression and production in RAW 264.7 cells To investigate the role of PDI in the regulation of proinflammatory cytokine TNF-α, we incubated ... bacitracin, a PDI inhibitor Finally, PDI expression was induced by the antiinflammatory cytokine IL-10, and IL-10-mediated inhibition of LPS-induced IL-6 expression was reduced by bacitracin These findings ... protein disulfide isomerase inhibition by short interfering RNA on tumor necrosis factor-alpha gene expression and release in RAW 264.7 cells To further confirm the role of PDI in the regulation...
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Báo cáo y học: "Evaluation of the effects of a VEGFR-2 inhibitor compound on alanine aminotransferase gene expression and enzymatic activity in the rat liver" pdf
Ngày tải lên :
13/08/2014, 13:20
... right and left branches of the portal vein [7] Insight on the potential distribution of drugs and toxins may help in understanding the potential localization of hepatic diseases and carcinomas ... effect on ALT gene expression In the right medial lobe, AG28262 treatment resulted in a 49% increase ALT gene expression compared to the control animals on day (Figure 1) Relative expression of ... Proteinase K (Dako) for 20 minutes The remaining steps including equilibration and end labeling reaction were followed per manufacturer’s protocol (Promega) Apoptotic cells were detected after incubation...
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Role of bcl 2 in metabolic and redox regulation via its effects on cytochrome c oxidase and mitochondrial functions in tumor cells
Ngày tải lên :
14/09/2015, 08:42
... increases in COX activity and oxygen consumption This observation corresponded to the maintenance of mitochondrial O2- levels in the Bcl-2 cells while the levels in nonoverexpressing cells continue ... conventional knowledge of the protein by focusing on the regulation of mitochondrial ROS production via its metabolic control on COX activity With this in mind, an increase in Bcl-2 expression ... HA14-1 increased mitochondrial respiration due to uncoupling and led to ROS production in CEM cells (Hao, Yu et al 2004) Indeed, we reported increased oxygen consumption in CEM/Neo cells treated in...
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Analysis of silicatein gene expression and spicule formation in the demosponge amphimedon queenslandica
Ngày tải lên :
13/10/2015, 14:07
... differentiated based on their meglasclere terminations and number of axes of symmetry, with demosponges having monaxons and tetraxons, and hexactinellids having monaxons and triaxons (Uriz et al 2003; ... encoding silicatein and cathepsin L reveals that exon length and exonintron border phase are highly conserved within most sponge silicatein genes and subset of cathepsin L genes 11 2.2 Introduction ... in A queenslandica silicatein genes (Table 2.1) Introns 1, 2, and are in phase 0, intron is in phase and intron in phase This intron phase sequence is also observed in S domuncula 20 and L baicalensis...
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Tài liệu Trade and Financial Integration in East Asia: Effects on Co-movements docx
Ngày tải lên :
17/02/2014, 21:20
... trade and financial integration on output, consumption and price co-movements Concluding remarks follow in Section TRADE AND FINANCIAL INTEGRATION IN EAST ASIA The export-led growth strategy in East ... Princeton Studies in International Finance, No 74 (International Finance Section, Princeton University) Eichengreen, B and Y C Park (2005a), ‘Why Has There Been Less Financial Integration in ... ‘Financial Integration and Consumption Risk Sharing in East Asia, Japan and the World Economy, 18, 2, 143–57 Kim, S., J.-W Lee and K Shin (2006), ‘Regional and Global Financial Integration in East...
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Tài liệu Báo cáo khoa học: Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix docx
Ngày tải lên :
20/02/2014, 23:20
... fractions, total cellular protein and chromatin-bound protein After the band intensities were quantitated in each protein, the concentrations of these proteins in total and chromatin-bound fraction ... (A) and the chromatin-bound fraction (B) from GM and WI-38 cells as in Fig Total proteins including histones were also detected by Coomassie Brilliant Blue staining decreasing concentrations ... cancer cells with deep invasion To further characterize the expression of Mcm4, PCNA and Ki67 in cancer cells, a section that contains a boundary region of CIS (CIN3 of FIGO classification) and dysplasia...
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Báo cáo khoa học: Recombinant bovine zona pellucida glycoproteins ZP3 and ZP4 coexpressed in Sf9 cells form a sperm-binding active hetero-complex ppt
Ngày tải lên :
07/03/2014, 05:20
... (lane in each panel), containing rZP4 expressed alone (lanes and in each panel), containing coexpressed rZP3 ⁄ rZP4 mixture (lanes and in each panel), containing rZP3FLAG expressed alone (lane in ... porcine and bovine ZP4 polypeptides differ in that an N-terminal region corresponding to residues 1–135 of bovine ZP4 (with the translation initiation Met numbered 1) is lacking in the porcine ... (0 min), indicating that rZP3 contains three N-linked chains After 24 h of digestion, rZP2 and rZP4 also migrated faster than undigested rZP2 and rZP4 (0 min), indicating that rZP2 and rZP4 contain...
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Báo cáo khoa học: The chromosomal protein HMGN2 mediates lipopolysaccharide-induced expression of b-defensins in A549 cells potx
Ngày tải lên :
22/03/2014, 16:20
... mRNA and protein expression HMGN2 mediates expression of b-defensins in Caco-2, HT-29 and SW480 cells [42] This is paralleled by changes in the acetylation of distinct core proteins, H4 and HMGN2, ... Mitogen-activated protein kinase kinase Mitogen-activated protein kinase Rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant) Rho-associated, coiled-coil containing protein kinase Ras-related ... crucial for LPS-induced HBD-2 expression in A549 cells The cells were incubated in DMEM with 10% FBS containing 0, 20, 40, 60, 80 or 100 lgÆmL)1 LPS for 24 h and then HBD-2 mRNA and protein levels...
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Báo cáo khoa học: Suppression of microtubule dynamics by benomyl decreases tension across kinetochore pairs and induces apoptosis in cancer cells potx
Ngày tải lên :
30/03/2014, 10:20
... resulting in the formation of the kinetochore fibre, leads to a reduction in the concentration of the motor proteins and spindle checkpoint proteins [19] This reduction of checkpoint proteins is ... Inhibition of proliferation (s) and mitotic progression (m) in HeLa cells by benomyl The inhibition of cell proliferation was determined by sulforhodamine B (SRB) assay after incubating the cells ... disassembled indicating a concentration dependent perturbation of microtubule–kinetochore interactions by benomyl (Fig 4) Benomyl caused a similar concentration dependent decrease in the quantity of spindle...
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Báo cáo hóa học: "Alloying and Strain Relaxation in SiGe Islands Grown on Pit-Patterned Si(001) Substrates Probed by Nanotomography" doc
Ngày tải lên :
21/06/2014, 20:20
... concentration increases monotonically for islands forming randomly on a flat surface, while it tends to a constant xGe = 0.4 for islands on the patterned substrate This result provides an independent confirmation ... major role in determining strain relaxation and therefore in defining the compositional profiles of the islands Experimental Procedure The sample considered here consists of 8.5 monolayer of Ge deposited ... reflecting the larger composition fluctuations Finally, the average Ge content of the individual islands increases monotonically with the island height (see Fig 3b) This behavior can be rationalized...
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Báo cáo y học: "Hypoxic conditions increase hypoxia-inducible transcription factor 2α and enhance chondrogenesis in stem cells from the infrapatellar fat pad of osteoarthritis patients" pps
Ngày tải lên :
09/08/2014, 10:20
... expressed in monolayer-cultured IPFP cells; this remained high in the chondrogenic cultures and there was no downregulation of its expression under hypoxic conditions The chondrogenic response of ... isothiocyanate-conjugated secondary antibody (green), and 4',6-diamidino-2-phenylindole (blue) Results showed strong staining for CD13, CD29, CD44, CD90 and CD105, weak staining for 3G5, and negative staining for ... response with additional changes in gene expression (Figure 3) In low oxygen the expression of HIF2α was increased 11fold over monolayer culture (Figure 3); interestingly, there was no change in...
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Báo cáo y học: " Basal core promoters control the equilibrium between negative cofactor 2 and preinitiation complexes in human cells" pdf
Ngày tải lên :
09/08/2014, 20:21
... motif combinations in TFIIB-dominated genes in illustrated in Figure 5e In line with the known synergy between INR and TATA [14], 94% and 56% of promoters harboring the INR motif also contained a ... which is in line with the factor’s original definition as a crucial PIC component [11] Conflicting reports indicating a negative TFIIB impact through BRE interactions (see Introduction) are not ... deviation of three independent reactions TFIIB and NC2 template association is expressed as percentage of relative binding, with the reaction showing maximum binding set to 100% with gene expression...
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Báo cáo y học: "Nipah virus infection and glycoprotein targeting in endothelial cells" ppsx
Ngày tải lên :
12/08/2014, 02:20
... findings indicate that NiV infection does not drastically influence endothelial permeability and barrier functions at early time points of infection Only after productive replication and pronounced ... shown in Figure 3B and 3C As in the infection (Figure 2), wildtype F was expressed bipolar upon single expression (Figure 3B; Fwt) Interestingly, mutations in both Y-based signals in the F protein ... in the F protein led to an apical redistribution in endothelial cells whereas only tyrosine 525 is involved in targeting in epithelial cells Neither the di-tyrosine nor the di-leucine motif in...
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Báo cáo y học: "Biochemical and morphological changes in endothelial cells in response to hypoxic interstitial edema" pdf
Ngày tải lên :
12/08/2014, 16:20
... and in control and in minor amount in fraction 7, while in hypoxia almost doubled in fractions and Lipid analysis in DRF Cholesterol was enriched in DRF in control (878 ± 80, nmol/mg prot) and ... Flotillin-1 content in fractions and was unchanged on comparing control to hypoxia Caveolin-1 was enriched in fraction in control, while it decreased about fold in hypoxia in this fraction; furthermore ... Protein contents of total plasma membranes Protein contents of total plasma membranes Caveolin-1, flotillin-1, AQP1, CD55 and actin contents in plasma membrane from tissue homogenates in control...
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Báo cáo y học: "Effect of the molecular adsorbent recirculating system and Prometheus devices on systemic haemodynamics and vasoactive agents in patients with acute-on-chronic alcoholic liver failure" potx
Ngày tải lên :
13/08/2014, 01:20
... effective circulating volume, which results in an activation of endogenous vasoconstrictor and water-retaining and sodium-retaining systems such as the renin–angiotensin–aldosterone system and the sympathic ... hormones during MARS treatment, suggesting either decreased production or elimination, or a combination of both Combined elimination and decreased production seems most probable We could demonstrate ... relation to inflammation (leukocytosis and C-reactive protein), to excretory and desintoxication function (ammonia, bile acids, bilirubin), to synthesis capacity (albumin, prothrombin time), to...
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Báo cáo y học: "Global analysis of alternative splicing regulation by insulin and wingless signaling in Drosophila cells" ppt
Ngày tải lên :
14/08/2014, 21:20
... Activation of insulin and wingless signaling pathways in Drosophila S2 cells Figure Activation of insulin and wingless signaling pathways in Drosophila S2 cells (a) Schematic representation of the insulin ... induced upon activation of the insulin and wingless pathways in S2 cells Binding of insulin-like peptides to the insulin receptor in Drosophila cells leads to the activation of dPI3 kinase, which Genome ... splicing patterns upon insulin and wingless activation To monitor changes in alternative splicing, the microarrays contain probes covering each reported exon-exon junction (splice-junction),...
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