... CGCTATTACCATGGTGATGC (nucleotides 4588–4608 of < /b> PCMV-Sport b- gal plasmid) CGGTTCACTAAACGAGCTCTGCTGCAGaaaaaatccaaaaaaaatctaaaaaaatcttttaaaa aaccccaaaaaaatttacaaaaaaGTCGACaatgc gcattGTCGACttttttgtaaatttttttggggttttttaaaagatttttttagattttttttg ... gcattGTCGACttttttgtaaatttttttggggttttttaaaagatttttttagattttttttg gattttttCTGCAGCAGAGCTCGTTTAGTGAACCG Ccttctccccggcggttagtgctgagagtgc aaaaaatccaaaaaaaatctaaaaaaatcttttaaaaaaccccaaaaaaatttacaaaaaa Primer with ... 200< /b> 8 The < /b> Authors Journal compilation ª 200< /b> 8 FEBS S Ma et al (1) β-gal CMV Transcription start site C β-gal (2) ARS-β-gal CMV Transcription start site β-gal AR S 5’-aaaaaatccaaaaaaaatctaaaaaaatcttttaaaaaaccccaaaaaaatttacaaaaaa-3’...
... of < /b> apoptosis (Annexin V+, 7AAD-) and (B) late stages of < /b> apoptosis (Annexin V+, 7AAD+); (C) CD8 lymphocytes in < /b> early stages of < /b> apoptosis (Annexin V+, 7AAD-) and (D) late stages of < /b> apoptosis (Annexin ... variation by season of < /b> the < /b> year We found that shipping of < /b> blood in < /b> insulated containers by contracted overnight carriers is < /b> associated with large seasonal variations in < /b> temperature inside the < /b> packaging, ... we find that there is < /b> function in < /b> the < /b> PBMC that are < /b> viable after overnight incubation Incubation at 15 or 30°C is < /b> associated with comparable T cell function assessed by ELIspot assay to that seen...
... programme High response rates are < /b> feasible because travelling distances are < /b> short in < /b> The < /b> Netherlands and regionalised high-risk perinatal care, including ECMO, is < /b> available The < /b> children are < /b> scheduled ... jugular vein are < /b> cannulated and subsequently ligated after bypass is < /b> finished Finally, the < /b> heparin that is < /b> administered to prevent the < /b> blood from clotting might cause intracranial haemorrhage as a < /b> ... intraventricular haemorrhage grade and grade Major: intraventricular haemorrhage grade and grade Nonhaemorrhagic intracranial abnormalities (n) Minor: ventricular dilatation and focal atrophy 17 Major:...
... whereas diazepam (N05BA01) is < /b> the < /b> only long-acting benzodiazepine The < /b> data were dichotomized as yes, taking the < /b> medication or no, not taking the < /b> medication of < /b> falling was measured using a < /b> single-item ... falls and dizziness the < /b> significant variables from the < /b> bivariate analyses at baseline were included as independent variables in < /b> multiple logistic regression analyses (manual backward), with data ... years < /b> 3 -years < /b> p = 0. 420 < /b> 6 -years < /b> p = 0 .406< /b> b Nagelkerke R2: under aged 80 0.138, 80+ years < /b> 3 -years < /b> 0.153 6 -years < /b> 0.167 c Variables at baseline entered into the < /b> regression analysis (manual backward):...
... that they can learn and develop further 5)wise in< /b> the< /b> handling of< /b> document • A < /b> good manager must know how in < /b> the < /b> processing data Maybe managers are < /b> afraid of < /b> cumbersome paperwork but the < /b> manager ... encouragement emanates from the < /b> Latin word Cor, meaning heart It means to give courage, confidence or hope Having courage is < /b> having the < /b> ability to disregard fear and to be brave The < /b> easiest way ... • Convey understanding, experience • Wise in < /b> the < /b> handling of < /b> documents • I)Qualities and skills of< /b> managers: • 1)management: • A < /b> manager's most < /b> important, and most < /b> difficult job is < /b> to manage...
... read the < /b> text and match the < /b> headings for paragraphs Paragraph A < /b> The < /b> tragic accident Paragraph BThe < /b> lift- off Paragraph C Congratulations Paragraph D A < /b> view on Earth Paragraph E Uncertainties In < /b> ... singing and dancing for them D taking to the < /b> baseball games Big Brothers is < /b> A < /b> the < /b> name of < /b> the < /b> club Ba < /b> home for children C the < /b> name of < /b> a < /b> film D on organization for boys who no longer have fathers ... comprehension is < /b> best described as an understanding between the < /b> author and the < /b> reader The < /b> emphasis is < /b> onthe < /b> reader understanding of < /b> the < /b> printed page based onthe < /b> individual reader’s unique background of...
... AAA and AAG by the < /b> arginine codons AGA and AGG respectively Rac1L61G189 was obtained by mutation of < /b> the < /b> cysteine codon TGC into the < /b> glycine codon GGC In < /b> all cases, the < /b> absence of < /b> additional mutations ... ubiquitination in < /b> the < /b> presence of < /b> Rac1L61 (Fig 3A,< /b> right panel), indicating that the < /b> stimulation of < /b> Rac1-dependent pathways may, in < /b> some way, activate the < /b> Rac1 ubiquitination machinery As Rac 1b has been ... ubiquitination that are < /b> not fulfilled by Rac 1b From our mutational analysis of < /b> Rac1 lysine residues, the < /b> main ubiquitin addition site in < /b> activated Rac1 appears to be Lys147; this result probably...
... expensive; therefore, they are < /b> a < /b> natural irst category to cut Only 7% of < /b> respondents are < /b> increasing investment in < /b> this area: 33% are < /b> not changing investment while 59% are < /b> decreasing it Print advertising ... publishing Case Studies, benchmark data, and how-to information read by hundreds of < /b> thousands of < /b> advertising, marketing and PR professionals every week Praised by The < /b> Economist, Harvard Business ... 47% are < /b> increasing spend on Web 2.0 strategies; only 8% are < /b> decreasing spend The < /b> low costs associated with these < /b> two tactics are < /b> probably one reason Lisa Mathisen, Owner, Realm Dekor, a < /b> small online...
... broad, black arrows indicate strands, and the < /b> black line indicates coiled, unstructured regions The < /b> bars at the < /b> location of < /b> the < /b> corresponding amino acids indicate the < /b> confidence of < /b> the < /b> secondary ... proportional to the < /b> number of < /b> amino acid substitutions Protein names, as listed in < /b> the < /b> respective database entries, if available, are < /b> shown before the < /b> species name UniProtKB accession numbers are < /b> ... genomic databases using a < /b> tailor-made bioinformatics facility The < /b> mascot search was run against all proteins and DNA sequence information from public databases FEBS Journal 273 (200< /b> 6) 4346–4359 ª 200< /b> 6...
... promoter activity is < /b> boxed, and the < /b> GAGA and Adf-1 elements in < /b> the < /b> DNA are < /b> underlined The < /b> translation start codon and the < /b> main transcription start point are < /b> larger and in < /b> bold (B) Mutations either in < /b> ... that are < /b> involved in < /b> mitochondrial energy-generating pathways and encoding components of < /b> the < /b> mitochondrial translation machinery [55] Adf-1 is < /b> a < /b> transcription factor containing a < /b> mybrelated DNA ... within a < /b> short element conserved in < /b> the < /b> transcriptionl initiation site of < /b> the < /b> bovine a-< /b> F1-ATPase gene The < /b> region located between the < /b> transcription initiation sites and the < /b> translation start codon...
... globule state of < /b> a-< /b> lactalbumin a-< /b> lactalbumin in < /b> the < /b> absence of < /b> aB-crystallin arises from aggregation of < /b> the < /b> molten globule state of < /b> this protein In < /b> the < /b> presence of < /b> aB-crystallin, the < /b> resonance ... the < /b> solution of < /b> R120G aB-crystallin and a-< /b> lactalbumin contained a < /b> heavy precipitate, whereas the < /b> mixture of < /b> wild-type aB-crystallin and a-< /b> lactalbumin was clear The < /b> sample of < /b> reduced a-< /b> lactalbumin, ... function by perturbing the < /b> putative role of < /b> the < /b> C-terminal extension in < /b> maintaining the < /b> integrity of < /b> the < /b> a-< /b> crystallin domain It is < /b> possible therefore that a < /b> combination of < /b> the < /b> above factors contributes...
... c-secretase activity was prepared by extracting carbonate-washed guinea pig brain membranes with 1% CHAPSO A < /b> mgÆmL)1 extract was diluted to obtain a < /b> final concentration of < /b> 0.5% CHAPSO, and dilutions ... dimethylsulfoxide in < /b> a < /b> concentration-dependent fashion Our results indicate that in < /b> vitro c-secretase cleavage of < /b> APP substrates is < /b> inhibited by zinc and that zinc increases the < /b> apparent molecular mass of < /b> C1013FLAG ... within APP C99 causes oligomerization to a < /b> noncleavable state An alternate explanation for the < /b> effect of < /b> zinc and copper inhibition is < /b> an interaction between metals and phospholipid bilayers Zinc...
... apps that can run onthe < /b> phone but also movies and books Most < /b> of < /b> the < /b> apps onthe < /b> market are < /b> free, and can be downloaded by simply touching the < /b> install button onthe < /b> app’s page in < /b> the < /b> market However, ... constrained to using your phone, navigating the < /b> market via a < /b> PC is < /b> often a < /b> good choice Without the < /b> constraints of < /b> a < /b> smartphone display you’ll be able to see more information about apps and navigate ... use their phone frequently as an MP3 player Yet another specific type of < /b> media management app is < /b> the < /b> podcast manager Podcasts are < /b> easy to download to phones, but managing them is < /b> another matter...
... of < /b> ANT From these < /b> experiments, we concluded that the < /b> ANT of < /b> our mitochondrial preparations of < /b> A < /b> franciscana embryos is < /b> fully functional ANT of < /b> A < /b> franciscana is < /b> refractory to inhibition by BKA ... a < /b> Ca2+-inducible PTP in < /b> embryos of < /b> A < /b> franciscana marks a < /b> cornerstone in < /b> our understanding of < /b> the < /b> long-term tolerance, extending for years,< /b> to anoxia and diapause, conditions that are < /b> invariably ... Yamada A,< /b> Yamamoto T, Yoshimura Y, Gouda S, Kawashima S, Yamazaki N, Yamashita K, Kataoka M, Nagata T, Terada H et al (200< /b> 9) Ca(2+)-induced permeability transition can be observed even in < /b> yeast...
... amino acids (aa), a < /b> short membrane-spanning region (21 aa) and an intracellular portion (567 aa), containing a < /b> kinase homology (KH) domain, the < /b> dimerization domain and the < /b> C-terminal catalytic ... dramatic increase in < /b> the < /b> phosphorylation at Ser487 Lastly, a < /b> functional role for phosphorylation at Ser487 in < /b> the < /b> ANPinduced inactivation of < /b> GC -A < /b> has been demonstrated by engineering a < /b> mutation ... cells), again suggesting that the < /b> population of < /b> receptors fully phosphorylated at these < /b> three additional positions was diminished Most < /b> remarkably, as already mentioned above, the < /b> amount of < /b> the < /b> two...
... were amassed The < /b> early 1990s predictions are < /b> available in < /b> a < /b> searchable database online at the < /b> site Imagining the < /b> Internet: A < /b> History and Forecast and they are < /b> also the < /b> basis for a < /b> book by Anderson ... elaborations have been edited Many full elaborations are < /b> included in < /b> the < /b> dozens of < /b> extra pages of < /b> detail included onthe < /b> Imagining the < /b> Internet online site Among the < /b> reports consulted as background ... what it will be in < /b> 15 years < /b> Data mining of < /b> personal traces is < /b> in < /b> its infancy Automatic facial recognition of < /b> video images is < /b> in < /b> its infancy Surveillance cameras are < /b> not all digital, nor are < /b> they...
... transcriptional factor subunits p50 and p65 This binding is < /b> specific and can be abolished by triple mutation of < /b> the < /b> oligonucleotide, as seen < /b> both in < /b> the < /b> direct binding and during competitive inhibition ... reagents including M199 and fetal bovine serum were obtained from Gibco-BRL (Bethesda, MD, USA) TNFa were obtained from Promega (Madison, WI, USA) All other reagents were obtained from Sigma ... which was then suppressed by cotreatment with NAC These < /b> findings indicate that both TNFa and H2O2 increased intracellular oxidative stress, which was then partially abolished by the < /b> antioxidant NAC...
... in < /b> the < /b> context of < /b> a < /b> satisfying lifelong bond Far from rejecting the < /b> value of < /b> commitment, almost everyone wants to create a < /b> lasting marriage or marriage-like relationship Their affirmation of < /b> the < /b> ... work amid mounting job demands and a < /b> lack of < /b> caretaking supports, they are < /b> developing second-best fallback strategies as insurance against their worst-case fears In < /b> contrast to their ideals, women’s ... single parents not question the < /b> advantages of < /b> being reared by two happily married parents But they also understand the < /b> obvious: happy marriages not break apart, and their own parents were not happily...