... was then added to each well, incubated at room temperature for 10 minutes, after which the optical density was read using a microplate reader set at 630 nm Statistical analysis All data are presented ... experimental animal for our research Results of this study demonstrate that PCV2 can replicate in Kunming mouse Kiupel [10] demonstrated that PCV2 was capable of replicating in BALB/c mice and caused ... has taken at least days for the PCV2 to be transferred into other organs from the primary interface It seems that the diffusion to thymus had been retarded for days The replication of PCV2 can...
... respectively, according to the manufacturer's protocol LDH leakage to the culture medium was measured with a CytoTox 96 nonradioactive cytotoxicity assay kit obtained from Promega (Madison, WI, USA) The ... either to proinflammatory or anti-inflammatory compounds together with or without LPS induction at DIV As a proinflammatory stimulus we used trichostatin A (TSA, 20 nM), a well characterised histone ... preparation of manuscript AS aided in study design, especially with regards to inflammatory experiments, supervised all experiments and helped to draft the manuscript All authors read and approved...
... high, saturated genetic maps would be necessary to have very close markers available each time in order to permit a molecular marker-assisted selection The current genetic maps contain around ... of around 80 chromosomes separated into two classes: a few distinguishable macrochromosomes and a much higher number of small microchromosomes, visualized as dots on metaphase preparations and ... genetic maps For this purpose, a collection of large insert BAC (bacterial artificial chromosome) and PAC (bacteriophage PI artificial chromosome) clones [80] to be used as microchromosome tags for...
... software packages The program SPARKY (T D Goddard and D G Kneller, University of California, San Francisco, USA) was used for the analysis of all NMR spectra Theory A classical approach toward ... important tool tostudy paramagnetic proteins Plastocyanin is a good model system to address features of paramagnetic copper proteins in terms of assignment strategy Indeed, the previously available assignment ... require any hardware or software dedicated to paramagnetic systems can substantially improve the available assignments close to the copper (II) ion without recourse to metal substitution Materials and...
... A state in this space is a partial alignment A transition is defined as the addition of a single link to the current state Any link which would create a state that does not violate any constraint ... parallel texts In Proceedings of the 4th Speech and Natural Language Workshop, pages 152–157 DARPA, Morgan Kaufmann Rebecca Hwa, Philip Resnik, Amy Weinberg, and Okan Kolak 2002 Evaluating translational ... algorithm that can handle many -to- one alignments This would involve training from an initial alignment that allows for many -to- one links, such as one of the IBM models Features that are related to multiple...
... from attacking dead targets (which also may lead to an overestimation of the damage to targeted systems) This too was not a limitation to the IOF Allocator but rather of the incoming data Finally, ... preprocessor again to create the input to the math solver The ability to make quick changes to the scenario also enables exploratory analyses and quick-reaction analyses Table B.1 Run-time Analysis ... a positive target Tanks are assigned a value of 10, and collateral targets are assigned a value equal to the negative of one tank (–10) There is an overkill factor of two in this example, meaning...
... Ras Another ability that is acquired by mammalian tumors is degradation of basement membranes Using an antibody to detect laminin and a collagen IV-GFP fusion protein to detect basement membranes, ... proliferation and metastasis [9,10] Scrib is an adaptor protein containing multiple PDZ domains (which enable binding toa variety of other proteins); it is localized to basolateral membranes of ... Richardson group [9] tested whether other signaling pathways can cause scrib mutant cells to metastasize They found that activation of the signaling pathways initiated by any of the extracellular...
... plant model available, Arabidopsis thaliana, because it is ideally suited to laboratory studies and there are extensive genetic and genomic resources available [20] Floral and foliar bioassays ... participated to Bd detached leaf assays AS and PN took part in designing and supervising the study and participated in drafting the manuscript All authors have read and approved the final manuscript ... study aims to examine the potential of Bd as amodeltostudy interactions with Fusarium species and a base from which to undertake modelto crop translational investigations Results Floral infection...
... non-linear and potentially paradoxical behavior [7,16,19] In order to aid in the qualitative characterization and examination of this relationship, we introduce the BIS, an agent-based model (ABM) based ... the access of the agents to Zone They also transmit signals produced in Zones and to attract agents to migrate The Portals also participate in the transport of some signals to Zone Portals are a ... it critically for important intellectual content The living authors, VAF and GCA, read and approved the final manuscript Additional material Additional file State diagram key A key to the symbols...
... respectively Total RNA was isolated and GAL1 mRNA was determined relative to ACT1 mRNA by quantitative real-time PCR The value for the un-induced cells was set as and the error bars indicate the standard ... RNA was isolated and GAL1 mRNA was determined relative to ACT1 mRNA by quantitative real-time PCR The value for the uninduced cells was set as and the error bars indicate the standard deviations ... FA Gal Glu HA HRP LB LiAc MMS NaAc PEG PMSF Raf SDS YPDA 3-amino-1,2,4-triazole 5-fluoroorotic acid Antimycin A Ampicillin Cycloheximide Carboxypeptidase Y Ethanol Formaldehyde Agarose Galactose...
... expectancies about a particular behavior in a given setting and can be operationalized as the likelihood to act (Fishbein & Ajzen, 1975) In astudy of potential travelers from mainland Korea to Jinju, Lam ... and the harbor, as well as to take advantages of various shopping malls The renowned Lan Kwai Fong area is located at the center of the bustling city, which houses many international restaurants, ... (CBs) to 0.91 (attitude), above the generally agreed upon lower limit of 0.70 (Hair, Anderson, Tatham, & Black, 2002) The scales can be used for analysis with acceptable reliability Factor analysis...
... all kinetoplastid CTs are unusual fusion proteins in having a cytidylyltransferase domain fused toa CDP-alcohol phosphatidyltransferase domain that is normally found in CEPT and CDP-ethanolamine:diacylglycerol ... in a mutant strain of Saccharomyces cerevisiae by the AUR1 gene J Biol Chem 272, 9809–9817 Kuroda M, Hashida-Okado T, Yasumoto R, Gomi K, Kato I & Takesako K (1999) An aureobasidin A resistance ... palmitoyl-CoA to form 3-ketosphinganine, a reaction catalysed by serine palmitoyltransferase After reduction of the product, dihydrosphinganine is N-acylated to dihydroceramide by a family of ceramide...
... thickness and inflammatory infiltration of synovial membrane in (b) and a normal appearance in (a) White arrows show depletion of proteoglycan (a marker for cartilage destruction) in (d) and a normal ... the statistical analysis DZ shared responsibility for manuscript preparation EA helped to perform the animal experiments ML performed the ELISA and L929 cytotoxic assay All authors read and approved ... were analyzed considering the area under the curve for each subject as a summary measure; these measures were then analyzed as raw data [19] According to data distribution and number of groups, a...
... contrast, the studies by Kawabata and Zeki (2004) and Skov ef al (2005) attempted to isolate those cortical structures that are actIvated more when a stimulus is evaluated as beautiful Presumably, ... processing along all five stages of the model The results indicate that evaluating a stimulus as beautiful was asSOCIated WIth mcreased activation in the orbital frontal cortex Activation in the orbital ... shown to be activated by emotions, and in particular by sahent stimuli about which one can form an affective impression rather automatically, such as faces or pictures from the International Affective...
... proliferation and invasion Choriocarcinoma is a malignant neoplasm that represents the early trophoblast of the attachment phase or as later invasive stage [46–48] Thus, in most cases, choriocarcinoma ... contrast to primary cultures of term cytotrophoblasts which aggregate and form syncytia In contrast to BeWo and Jeg3 cell lines, JAr cells share many of the characteristics of early placental trophoblasts, ... HDL preparation used that would be necessary to deliver the observed amount of tracer These calculations are performed to compare uptake of 125I- and H-tracers on the same basis In a parallel set...
... (nonauto) Plastics manufacturing (auto) 26 Metal-related Metallurgical, metalworking, metal fabrication 64 75 Transportation Transportation 37 26 Cleaning/beauty care Beauty salon/hair care 25 ... Luginaah I, Laukkanen E, Hellyer D, Reinhartz A, Watterson A, Abu-Zahra H, Maticka-Tyndale E, Schneider K, Beck M, Gilbertson M: Occupation and breast cancer: a Canadian case– control study Ann ... large retail sector as reference (data not shown) That sector appeared to have less than average breast cancer risk (Tables 1, 3) and, as a result, all the estimates for other sectors increased considerably...
... plays a key role, money has no accounting relationship to any other variable The model also contains no explicit analysis of what happens when either goods and services or financial assets are ... economies reach a quasi-stationary state in which all stocks and all flows including the stock of money not change at all – but there is a never ending purchase of Treasury Bills (and a growing stock ... appropriate use of monetary and fiscal policy for internal and external stability’, International Monetary Fund Staff Papers, (March), 70-77 Mundell, R (1963), ‘Capital mobility and stabilization...