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Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf
Ngày tải lên :
18/02/2014, 13:20
... primers: Aba, 5Â-ATGGACGCTGAAT
TCCGTCACGACTCTGGTTACGAAGTTCACCACCAG
AAGCTGGTG-3Â;Abb, 5Â-GTTCACCACCAGAAGCT
GGTGTTCTTC GCTGAA GACGT GGGTTCT AACAAG
GGTGCT-3Â;Abc, 5Â-CACAACGCCACCAACCATCAGA
CCGATGATAGCACCCTTGTTAGAACCCAC-3Â;Ab-
start, ... 5Â-CACAACGCCACCAACCATCAGA
CCGATGATAGCACCCTTGTTAGAACCCAC-3Â;Ab-
start, 5Â-GCGTAGGGTCGACATATGGACGCTGAATT
CCGTCACG-3Â;Abstop, 5Â-CCTGCCGAGCTCCTATTA
CACAACGCCACCAACCATCAG-3Â.
The PCR solution was prepared in the buffer supplied
with ... (F19P, A2 1G, E22G, E22K, E22Q and
D23N), and the availability of a rapid and simple
expression and purification protocol will facilitate
large-scale investigations of the molecular determinants
of aggregation...
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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
Ngày tải lên :
18/02/2014, 17:20
... tsA58T Ag cDNA carry-
ing the A4 38V mutation were PCR-amplified from COS-7
cDNAs using the following primers: LTA-1F, 5Â-CTC
GAGATGGATAAAGTTTTAAACAGAG-3Â and LTA-
1R, 5Â-TGAAGGCAAATCTCTGGAC-3Â for ... isolation and long-term
culture of organ-specific blood vascular and lymphatic
endothelial cells of the mouse
Takashi Yamaguchi, Taeko Ichise, Osamu Iwata, Akiko Hori, Tomomi Adachi, Masaru Nakamura,
Nobuaki ...
75 kDa
25 0
kDa
150
kDa
100
kDa
100 kDa
75 kDa
50
kDa
100 kDa
75 kDa
Lyve-1
Prox-1
VEGFR-3
tsA58 T Ag
/ tublin
Lyve-1 DaAPI
DaAPI Lyve-1 Prox-1
CDa31 DaAPI
Magnetic
A
B...
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Báo cáo khoa học: "A Nonparametric Method for Extraction of Candidate Phrasal Terms" docx
Ngày tải lên :
08/03/2014, 04:22
... 605–613,
Ann Arbor, June 20 05.
c
20 05 Association for Computational Linguistics
A Nonparametric Method for Extraction of Candidate Phrasal Terms
Paul Deane
Center for Assessment, Design and Scoring ... Annual Meeting
of the Association for Computational Linguistics,
pages 188-195.
Ferreira da Silva, J. and G. Pereira Lopes (1999). A
local maxima method and a fair dispersion
normalization for ... unigram
distributions, and generalizations to bigram and n-
gram distributions on large corpora are not as yet
clearly feasible (Baayen 20 01 :22 1). Yet many of
the best-performing lexical association...
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History Of Egypt, Chaldæa, Syria, Babylonia, and Assyria, Volume 2 (of 12) pptx
Ngày tải lên :
08/03/2014, 22:20
... ocean. Some are mentioned as being able to divide the waters at their will, and to cause them to
return to their natural place, merely by means of a short formula. An image of a man or animal made ... or a she-ass.
Smaller retail bargains did not demand so many or such complicated calculations. Two townsfolk stop for a
moment in front of a fellah who offers onions and corn in a basket for sale. ... celebrated lawsuit, some details of which are preserved for us
in a papyrus of Turin, gives us some information in regard to a conspiracy which was hatched in the harem
against Ramses II.
** A passage...
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The Finite Element Method Fifth edition Volume 2: Solid Mechanics.Professor O.C. Zienkiewicz, CBE pot
Ngày tải lên :
14/03/2014, 15:20
... problems
Appendix C. Basic equations of displacement analysis
Appendix D. Some integration formulae for a triangle
Appendix E. Some integration formulae for a tetrahedron
Appendix F. Some vector algebra
Appendix ... explicit
dynamic transient analysis (see Chapter 18 of Volume 1) is carried out to achieve a
steady-state solution.
32; 33
These forms are often characterized by:
1. a diagonal or very sparse form of the matrix ... of transient non-linear calculations 19
disadvantage of a line search is the need for several evaluations of É. However, the
acceleration of the overall convergence can be remarkable when applied...
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Aptitude for Destruction, Volume 2 - Case Studies of Organizational Learning in Five Terrorist Groups pptx
Ngày tải lên :
22/03/2014, 23:20
... to develop and use chemical agents, Aum’s early struggles against
rival organizations reveal how it managed relations with others whom Asahara and
his close associates viewed as obstacles to their ... group are particularly significant: First, Aum was the crea-
tion of Shoko Asahara, its founder, charismatic leader, and spiritual guru. Second,
Aum learned how to raise money, ultimately amassing ... contacts that
it eventually exploited to procure weapons capabilities. For example, Aum had two
companies that purchased chemicals for its chemical weapons programs without at-
tracting unwanted...
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Báo cáo khoa học: Kinetic hybrid models composed of mechanistic and simplified enzymatic rate laws – a promising method for speeding up the kinetic modelling of complex metabolic networks pptx
Ngày tải lên :
23/03/2014, 06:20
... physiologically feasible
ranges:
1
2
k
0
ATPase
k
ATPase
2k
0
ATPase
(small variation of the energetic load)
1
5
k
0
ATPase
k
ATPase
5k
0
ATPase
(large variation of the energetic load)
1
50
k
0
ox
... tissue [25 ,26 ]. Finally, if
regulatory effectors have been elucidated by careful
kinetic characterization of an enzyme, there are suffi-
cient data available to set up a mechanistic rate law
instead ... not
include all reactions that have been reported in the
Table 5. Ranking of saturation parameters for hepatocyte purine
metabolism. Average ranking of saturation parameters according to
their impact...
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USAID Supported a Wide Range of Child and Maternal Health Activities, but Lacked Detailed Spending Data and a Proven Method for Sharing Best Practices pptx
Ngày tải lên :
28/03/2014, 09:20
... 9 ,20 7
Honduras 3,1 42 3,143 3,377
India 12, 600 14 ,22 2 12, 8 52
Indonesia 11,400 13,800 14,157
Jamaica 544 539 497
Kenya 1,000 1,000 989
Liberia 1 ,20 0 1 ,20 0 1,5 82
Madagascar 2, 825 3,475 3 ,28 7
Malawi ... 8,601
Dominican Republic 4,000 3,861 3 ,23 7
El Salvador 2, 700 2, 970 2, 970
Eritrea 1,600 5
a
0
a
Ethiopia 4,600 6,090 7 ,25 7
Ghana 3 ,20 0 3 ,20 0 2, 719
Guatemala 4,150 4 ,21 5 4,158
Guinea 2, 150 2, 150 2, 200
Haiti ... Allocation of CS/MH Account
Funds to Countries, Fiscal Years 20 04 and
20 05
Fiscal year
Country 20 04 (actual) 20 05 (actual) 20 06 (planned)
Afghanistan $16,870 $19,870 $21 ,005
Angola 2, 700...
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Báo cáo khoa học: "A Bayesian Method for Robust Estimation of Distributional Similarities" pot
Ngày tải lên :
30/03/2014, 21:20
... A hierarchical Bayesian lan-
guage model based on Pitman-Yor processes. In
Proceedings of COLING-ACL 20 06, pages 985–9 92.
Akira Terada, Minoru Yoshida, and Hiroshi Nakagawa.
20 04. A tool for constructing ... Estimation of Distributional Similarities
Jun’ichi Kazama Stijn De Saeger Kow Kuroda
Masaki Murata
†
Kentaro Torisawa
Language Infrastructure Group, MASTAR Project
National Institute of Information ... 48th Annual Meeting of the Association for Computational Linguistics, pages 24 7 25 6,
Uppsala, Sweden, 11-16 July 20 10.
c
20 10 Association for Computational Linguistics
A Bayesian Method for Robust...
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Báo cáo khoa học: "A Unification Method for Disjunctive Feature Descriptions" pot
Ngày tải lên :
31/03/2014, 17:20
... conjuncts may be changed.
The unconditional conjuncts of a formula contain informa-
tion that is more definite than the information contained in
disjunctions. Thus a formula can be regarded as having ... Press, Cam-
bridge, England, 1985.
[8] Perelra, F. C. N. and D. H. D. Warren. Definite clause
grammars for language analysis - a survey of the formal-
ism and a comparison with augmented transition ... disjunctions, if any at all. Most disjunctions in a systemic
grammar represent possible alternative values that some par-
ticular feature may have (along with the grammatical conse-
quences entailed...
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a novel method for the synthesis of titania nanotubes using
Ngày tải lên :
05/05/2014, 15:26
... the annealed titania nanotubes
are crystalline (mostly anatase) and show varied activity de-
pending on the material preparation and annealing atmosphere
(Fig. 12) .
Titania nanotubes prepared ... stirring and (b) ultrasonic.
S.K. Mohapatra et al. / Journal of Catalysis 24 6 (20 07) 3 62 369 367
Fig. 10. DRUV–vis spectra of (a) O
2
annealed UAT, (b) N
2
annealed UAT,
(c) as-prepared UAT, and ... respectively.
2. 3. Annealing of the materials
The anodized titania nanotubular arrays were annealed in a
nitrogen and oxygen atmosphere at 500
◦
Cfor6hinaCVDfur-
nace at a heating rate of 1
◦
C/min. The UAT samples...
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a novel method for the synthesis of cao nanoparticle
Ngày tải lên :
06/05/2014, 08:55
... There are several methods for the synthesis
of nanoscale CaO, including sol-gel [21 ], gas
phase condensation [21 ], laser ablation [21 ],
ame processing [22 ], sonochemical,
microwave plasma [23 ], ... chromatograms in the presence of different weight ratios and isopropanol solvent.
Surface ratio% or %
decompose
Surface ratio(AUC 2/ AUC 1)AUC /2- CEPS (2) AUC/Toluene(1)Ratiosample
1000. 928 027 337 529 4585BlankA
91.370.847 923 793 528 06171:10B
75.90
0
.7043
24 6553
3
50069
1 :20
C
59.310.550 320 31 123 690951:30D
24 .710 .22 93803593504561:40E
Figure ... (1997).
[21 ] J .A. Rodriguez, M. Fernandez-Garcia,
Micro. Charac. Mater., 32, 455 (20 07).
[22 ] J.G. Lu, P. Chang, Z. Fan, Mater. Sci.
Eng. R., 52, 49 (20 06).
[23 ] A. Gedanken, Current Science, 85, 12...
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Báo cáo hóa học: "One Step Nucleic Acid Amplification (OSNA) - a new method for lymph node staging in colorectal carcinomas" docx
Ngày tải lên :
18/06/2014, 16:20
... Cancer 20 08, 122 :25 62- 7.
22 . Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N,
Hase T: Loop-mediated isothermal amplification of DNA. Nucleic Acids Res
20 00, 28 :E63.
23 . Weitz ... sequences for amplification of CEA were: 5’ -
AGACAATCACAGTCTCTGCGGA-3’ (forward) and
5’ - ATCCTTGTC CTCCACGGGTT-3’ (reverse). The
cut-off was set at cycle time = 29 .6 for CK19, 28 .5 for
CEA, and 30.0 ... levels for each of 2 LN slices). RNA quality was
assured by OSNA performed for beta-actin. 139 samples
gaveanegativeresultand37samplesgaveapositive
result with both methods (table 2) . No isolated...
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Báo cáo sinh học: " A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" potx
Ngày tải lên :
19/06/2014, 08:20
... times a week.
The database will be made available free of charge to inter-
ested parties.
Table 7: Amplification efficiency for patients' amplicons
Genotype 1a
Amplicon A1 A2 A3 A4 x A4 y
Amplification ... K: Hypervariable regions in the putative glycoprotein
of hepatitis C virus. Biochem Biophys Res Commun 1991,
175 :22 0 -22 8.
8. Kato N, Ootsuyama Y, Tanaka T, Nakagawa M, Nakazawa T, Muraiso
K, Ohkoshi ... sequences and conserved
secondary structures at the 3' end of HCV genome and its
implication for viral replication. Nucleic Acids Res 19 92, 20 :3 520 .
7. Hijikata M, Kato N, Ootsuyama Y, Nakagawa...
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