a biogas plant seldom
Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt
... coronariaRaka Ghosh, Sibani Chakraborty, Chandana Chakrabarti, Jiban Kanti Dattagupta andSampa BiswasCrystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, Kolkata, ... acidat a particular position for this family of plant cysteine pro-teases. The primers used were 5¢-TTGCCTGAGCA TGTTGATTGGAGAGCGA AAG-3 ¢ (forward) and 5¢-GGGATAATAAGGTAATCTAGTGATTCCAC-3¢ ... S, Sundd M, Jagan-nadham MV & Dattagupta JK (1999) Crystallizationand preliminary X-ray analysis of ervatamin B and C,two thiol proteases from Ervatamia coronaria. ActaCrystallogr D 55,...
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Tài liệu Báo cáo khoa học: Spectroscopic characterization of a higher plant heme oxygenase isoform-1 from Glycine max (soybean) ) coordination structure of the heme complex and catabolism of heme docx
... vanishedafter 30 min, and instead, an absorption peakappeared at 637 nm, suggesting the formation ofCO–verdoheme. The 637 nm band disappeared gradu-ally and was replaced by a new broad band ... T, Zhang X, Sun D,Sato M, Sasahara M, Kayama T, Ikeda-Saito M &Yoshida T (2000) Histidine 20, the crucial proximalaxial heme ligand of bacterial heme oxygenase Hmu Ofrom Corynebacterium ... specific absorption bands. Heme catabolism byHOs of mammals, pathogenic bacteria, cyanobacteriaand probably insects is considered to have a similarmechanism, because the characteristic absorptionbands...
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Báo cáo y học: "A Comparative Effectiveness Study of Bone Density Changes in Women Over 40 Following Three Bone Health Plans Containing Variations of the Same Novel Plant-sourced Calcium"
... investigators‟ DXA (Total Body Du-al-energy X-ray Absorptiometry) database, from par-ticipants at a local health fair, and from referrals from subjects who agreed to participate. All subjects ... known as „AlgaeCal‟ or „AC‟) made by milling whole, live-harvested sea algae found on the South American coastline. In addition to calcium, the algae contained other naturally occurring minerals ... 61. Marone PA, Yasmin T, Gupta RC, et al: Safety and toxicological evaluation of AlgaeCal (AC), a novel plant- based calcium sup-plement. Toxicol Mech Methods. 2010; 20:334-344. 62. Kaats GR:...
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Boosting biogas yield of anaerobic digesters by utilizing concentrated molasses from 2nd generation bioethanol plant
... meet the seasonal variation in energy demand. Biogas plant connected with CHP (combined heat and power plant) is typically designed for base load due inadequacy of the material characterized ... dietary fibres and klason lignin was analysed as described by Knudsen [13] whereas mineral analysis (primarily cations and anions) was conducted as according to Fang et al [14]. Fat was determined ... glucose and arabinose (Table 1). Although not abundant, small amount of other sugars such as rhamnose (<1g/kgTS), mannose and galactose were also observed. Furthermore, analysis quantified approximately...
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Tài liệu Báo cáo khoa học: C-Terminal extension of a plant cysteine protease modulates proteolytic activity through a partial inhibitory mechanism doc
... aa208 aa24 aaBamH34 aaStartStopXho1N-Pro Protease domainProtease domainCT - ex380 aa1IINP114 aa208 aaBamH134 aaStartStopXho1SS SSGSSSSN-Pro356 aaIIIMSTLFIISILLFLASFSYAMDISTIEYKYDKSSAWRTDEEVKEIYELWLAKHDKVYSGLVEYEKRFEIFKDNLKFIDEHNSENHTYKMGLTPYTDLTNEEFQAIYLGTRSDTIHRLKRTINISERYAYEAGDNLPEQIDWRKKGAVTPVKNQGKCGSCWAFSTVSTVESINQIRTGNLISLSEQQLVDCNKKNHGCKGGAFVYAYQYIIDNGGIDTEANYPYKAVQGPCRAAKKVVRIDGYKGVPHCNENALKKAVASQPSVVAIDASSKQFQHYKSGIFSGPCGTKLNHGVVIVGYWKDYWIVRNSWGRYWGEQGYIRMKRVGGCGLCGIARLPYYPTKAAGDENSKLETPELLQWSEEAFPLAIV A B66 ... of a plant cysteine proteasemodulates proteolytic activity through a partial inhibitorymechanismSruti Dutta, Debi Choudhury, Jiban K. Dattagupta and Sampa BiswasCrystallography and Molecular ... protease from the latex of Ervatamia coronaria.Biosci Biotechnol Biochem 62, 1947–1955.15 GuhaThakurta P, Biswas S, Chakrabarti C, Sundd M,Jagannadham MV & Dattagupta JK (2004) Structuralbasis...
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Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc
... QuikChange site-directedmutagenesis kit (Stratagene, La Jolla, CA, USA). PlasmidpET-1TK was used as template and Kleb(HtoA)fw(5¢-GCTTAGCCGCGCCGGCATTCG) and Kleb(HtoA)rv(5¢-CGAATGCCGGCGCGGCTAAGC) ... glucose-1-phosphatase and human prostatic-acid phosphatase. The polypeptidechain is organized into an a and an a ⁄ b domain, and the active site islocated in a positively charged cleft between the domains. ... phytate with pH optima in theacidic range. They consist of two domains, a large a ⁄ bdomain and a small a domain with the catalytic site atthe interface of the two domains [4,5]. HAPs can initi-ate...
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Tài liệu BIOCHEMICAL TARGETS OF PLANT BIOACTIVE COMPOUNDS A pharmacological reference guide to sites of action and biological effects doc
... in plants used for stimulatory drinks such as Ilex paraguayensk (matC) (Aquifoliaceae), Coffea species (coffee) (Rubiaceae), Paullinia cupana (guarana) (Sapindaceae), Cola acuminata (cola) and ... alkaloids are or-glycosidase inhibitors, namely (sources in parentheses) alexine (Alexa leiopetala (Fabaceae)), australine (Castanospermum australe (Fabaceae)) and casuarine (Casuarina ... Artemisia annua (Asteraceae) is of major importance as an antimalarial because of extensive resistance of Plasmnodium falciparum to antimalarials such as chloroquine. Artemisinin in has a...
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Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt
... fact that EDTAeffects a marked stabilization (Table 1), however, indi-cates an additional destabilizing effect by calcium ions.Destabilization of PLDa2 by calcium ions was alsoindicated by ... protein absorbance at 280 nm. Aldolase (150 kDa),ovalbumin (45 kDa), chymotrypsinogen A (25 kDa), andcytochrome c (12.3 kDa) (Serva, Heidelberg, Germany)were used as molecular mass standards.Absorption ... (w ⁄ v) acrylamide] were stainedwith Coomassie Brilliant Blue G250 and quantified bydensitometric evaluation at 595 nm (CD60; Desaga, Darm-stadt, Germany).Small angle X-ray solution scattering...
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Tài liệu Báo cáo khoa học: 1,5-Diamino-2-pentyne is both a substrate and inactivator of plant copper amine oxidases ppt
... experimentsperformed according to that with DABY-inactivated GPAO[8] revealed that the pI value of the DAPY-inactivatedGPAO was not dramatically changed. The native GPAOis characterized by a pI of 7.2 [18]. After ... 1,5-diamino-2-pentyne(DAPY). The unsymmetrical DAPY comprises both a propargyl and homopropargyl amine.DAPY was synthesized and tested as a substrate of two plant CAOs. DAPY acts as a mechanism-based inhibitor ... mixture prepared in 0.1Mammonium bicarbonate containing ACA as a reagent fortrapping of the product aminoaldehyde, where several newpeaks appeared. ACA itself is represented by a peak withm/z...
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Tài liệu Báo cáo khoa học: Plant a-amylase inhibitors and their interaction with insect a-amylases ppt
... inhibitor;HSA, human salivary a- amylase; LCAI, Lachrima jobi chitinase/ a- amylase inhibitor; PAI, pigeonpea a- amylase inhibitor; PPA, por-cine pancreatic a- amylase; RASI, rice a- amylase/subtilisin ... bean; AMY1and AMY2, a- amylases from barley seeds; BASI, barley a- amylasesubtilisin inhibitor; BLA, Bacillus licheniformis a- amylase; CAI,cowpea a- amylase inhibitor; CHFI, corn Hageman factor ... Brazil, Fax: + 5 5 6 1 340 3624,Tel.: + 55 61 448 4705, E-m ail: ocfranco@cenargen.embrapa.brAbbreviations: AAI, Amaranthus a- amylase inhibitor; a- AI1 and a- AI2, a- amylase inhibitors 1 and 2 from...
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Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf
... L, Hamid Q & Elias JA (2004) Acidicmammalian chitinase in asthmatic Th2 inflammationand IL-13 pathway activation. Science 304, 1678–1682.4 Kasprzewska A (2003) Plant chitinases ) regulation ... 491–503.24 Aronson NN, Halloran BA, Alexyev MF, Amable L,Madura JD, Pasupulati L, Worth C & Van Roey P(2003) Family 18 chitinase–oligosaccharide substrateinteraction: subsite preference and anomer ... demonstrated by kineticand molecular modeling studies. Plant Mol Biol 52, 43–52.26 Ueda M, Kojima M, Yoshikawa T, Mitsuda N, ArakiK, Kawaguchi T, Miyatake K, Arai M & Fukamizo T(2003) A novel...
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Báo cáo khoa học: Epl1, the major secreted protein of Hypocrea atroviridis on glucose, is a member of a strongly conserved protein family comprising plant defense response elicitors potx
... Comparini C, Calamassi R, Pazzagli L,Cappugi G & Scala A (2004) Cerato-platanin protein islocated in the cell walls of ascospores, conidia andhyphae of Ceratocystis fimbriata f. sp. Platani. ... 341–346.25 Pazzagli L, Cappugi G, Manao G, Camici G, Santini A & Scala A (1999) Purification, characterization, andamino acid sequence of cerato-platanin, a new phyto-toxic protein from Ceratocystis ... Stability ofclades was evaluated by 1000 bootstrap rearrangements.Bootstrap values lower than 20% are not displayed in thecladogram.RNA isolation and hybridizationFungal mycelia were harvested...
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Báo cáo khoa học: Plant DNA polymerase k, a DNA repair enzyme that functions in plant meristematic and meiotic tissues docx
... Nagasawa, K., Kitamura, K., Yasui, A. , Nimura, Y., Ikeda, K.,Hirai, M., Matsukage, A. & Nakanishi, M. (2000) Identificationand characterization of human DNA polymerase b2, a DNApolymerase ... 5¢-AGCTACCATGCCTGCACGAAGAGTGCGTATTATGCCTACACTGGAGTACCGGAGCATCGTCGTGACTGGGAAAAC-3¢.[3H]dTTP (10 lM;10CiÆmmol)1) and enzymes were addedas indicated in the figure legends, and incubated ... visualized by autoradiography.DNA substrate used in this assay is a d15:d21-mer primer/template. The sequences are 5¢-ACTGGAGATCTGCAT-3¢ and 5¢-TGAAGCATGCAGATCTCCAGT-3¢.Misincorporation assayThe...
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Báo cáo khoa học: Purification of a plant nucleotide pyrophosphatase as a protein that interferes with nitrate reductase and glutamine synthetase assays pdf
... var. botrytis) extracts. The finalpreparationcontainedanacyl-CoAoxidaseandasecondprotein of the plant nucleotide pyrophosphatase family. ThispreparationhydrolysedNADH,ATPandFADtogenerateAMP ... Sonoda, M., Ide, H., Nakayama, S., Sato, T. & Nakagawa, H.(2000) Cloning and expression analysis of nitrate reductaseinactivator (NRI) gene from spinach. Plant Cell Physiol. 41, s160.12. Kaiser,W.M.,Weiner,H.,Kandlbinder ,A. ,Tsai,C.B.,Rockel,P., ... (v/v) HCl and 24%(w/v) trichloroacetic acid. The A 504was measured and thec-glutamyl hydroxamate produced was quantified usingcommercial c-glutamyl hydroxamate as standard. Controlassays were...
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Báo cáo khoa học: Isolation and enzymatic characterization of lamjapin, the first ribosome-inactivating protein from cryptogamic algal plant (Laminaria japonica A) ppt
... ribosomal RNA by lamjapin were quantita-tively analyzed by the chloroacetaldehyde method. A timecourse study demonstrated that the release of adenine bylamjapin continued at a linear rate for at ... the firstribosome-inactivating protein from cryptogamic algal plant (Laminaria japonica A) Ren-shui Liu1, Jia-hua Yang2and Wang-Yi Liu11State Key Laboratory of Molecular Biology, Institute ... of rat ribosomal RNAwas flipped [27]. Lamjapin could deadenylate A2 0 of SRDRNAandreleasedtheRNAfragmentwiththeexactsizeofthe R-fragment from the ribosomal RNA. It is likely thatlamjapin deadenylates...
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- a plant protein storage vacuole sorting receptor
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