5 analysis of neural cell functions in gene knockout mice electrophysiological investigation of synaptic plasticity in acute
Báo cáo y học: "Frequency analysis of TRBV subfamily sjTRECs to characterize T-cell reconstitution in acute leukemia patients after allogeneic hematopoietic stem cell transplantation" pot
... Science Foundation of Hainan Province of China (3 052 0) Page of Author details Institute of Hematology, Medical College, Jinan University, Guangzhou 51 0632, PR China 2Department of Hematology, Nanfang ... Guangzhou 51 051 5, PR China 3Department of Hematology, Guangdong General Hospital, Guangzhou 51 0080, PR China Department of Hematology, Hainan Province People’s Hospital, Haikou 57 0311, PR China Authors’ ... frequencies of CD45RA + /CD4 + , CD45RA + /CD8 + , and CD45RO + /CD4 + T cells after HSCT are shown in Figure In the early period after HSCT (within 12 weeks), the frequencies of CD45RA + /CD4+, CD45RA+/CD8+,...
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Elucidating the role of dok 3 in b cell receptor signaling using gene knockout mice 2
... Catalog number 01122D 55 3 159 01715B 55 3111 12-0 251 -83 01602D 01035A 0 150 2D 55 3691 SC-2020 02082D 55 9749 SC-20 05 L-0881 SC-2004 55 3406 406604 407104 Anti-B220-Biotin Anti-BP-1-Biotin Anti-CD11b-PE ... SC-104 75 SC- 154 SC-474 SC- 15 SC-371 SC-728 SC-7383 3941 Goat Goat Rabbit Rabbit Rabbit Rabbit Rabbit Mouse Rabbit 1 :50 0 1 :50 0 1 :50 0 1 :50 0 1 :50 0 1:1000 1 :50 0 1 :50 0 1 :50 0 Cell Signaling Cell Signaling ... by providing specific binding site for Src-homology (SH) domain containing effectors, including several classes of cytoplasmic protein tyrosine kinases (PTKs), which phosphorylate intracellular...
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Elucidating the role of dok 3 in b cell receptor signaling using gene knockout mice
... 2.3.14 2.3. 15 2.3.16 48 52 53 54 54 55 56 56 56 57 57 58 58 59 59 2.4 Buffers and solutions Mouse tail genomic DNA prep Genotyping of Dok-3 mice Polymerase chain reaction DNA sequencing Restriction ... 2 .5. 1 Buffers and solutions 63 iii 2 .5. 2 2 .5. 3 2 .5. 4 2 .5. 5 2 .5. 6 2 .5. 7 2 .5. 8 2 .5. 9 2 .5. 10 2 .5. 11 2.6 Cloning of targeting construct Culturing of mouse embryonic fibroblasts Culturing of ES cells ... Extracellular-signal-regulated kinase Embryonic stem Florescence activated cell sorting Fluorescein isothiocyanate Interleukin Four Receptor Interacting Protein RasGAP SH3 domain binding protein Geneticin Gancyclovir...
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báo cáo hóa học:" Microarray analysis of Foxl2 mediated gene regulation in the mouse ovary derived KK1 granulosa cell line: Over-expression of Foxl2 leads to activation of the gonadotropin releasing hormone receptor gene promoter" pptx
... mammalian Sin3 co-repressor protein [10,11] The effectiveness of the Sin3 binding domain of Mad in silencing gene expression when fused to DNA binding proteins has been demonstrated in two studies ... +5. 3 Serpine2 serine (or cysteine) peptidase inhibitor, clade E, member 20720 MR +2.4 Snw1 SNW domain containing 66 354 E16, P0 Sox21* SRY-box containing gene 21 223227 MR +2 .5 Zfp106 zinc finger ... laboratory developed the cell line and from Dr Deborah Segaloff who sent us the cell line The cells are grown in DMEM/F12 (50 /50 ) containing 10% heat inactivated FBS (Gemini Bioproducts; West Sacramento,...
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báo cáo khoa học: "Analysis of the expression pattern of the BCL11B gene and its relatives in patients with T-cell acute lymphoblastic leukemia" doc
... amounts of the genes of interest and the b2M reference gene were measured in two independent assays The 2(-ΔΔCT) method was used to present the data of the genes of interest Huang et al Journal of ... reference gene b2-MG were determined by SYBR Green I real-time PCR Briefly, PCR was performed in a 25- μL total volume containing μL of cDNA, μL of 2 .5 SYBR Green mix (Tiangen, Beijing, PR China), ... b2M reference gene C: Melting curves of the BCL2L1 and b2M genes from nine patients #: Specific peak of the b2M reference gene begins at 81°C ##: Specific peak of the BCL2L1 gene begins at 84°C...
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Báo cáo y học: "Analysis of variation of amplitudes in cell cycle gene expression" potx
... ) of the amplitudes in the 2nd and 4th experiments of Whitfield et al (2002), respectively Assession AA088 457 AA 458 994 AA4 854 54 AA4 854 54 AA2829 35 N57722 AA4 854 54 AA4 854 54 R11407 T669 35 AA 452 513 ... 0. 052 0.031 -0.637 -0.6 35 -0 .55 4 -0 .55 4 -0. 658 -0.180 -0 .55 1 -0 .55 1 -0.467 -0.903 -0.609 -0. 650 -0. 755 -1.144 -0.387 -0.738 -0.329 -0.378 -0.6 35 -0.6 05 -0.192 -0 .59 7 0.007 -0.280 -0.378 -0 .59 2 ... -0.670 -0 .50 0 -0.702 0.0 05 -0.177 -0.1 45 -1.067 -1.072 -0.893 -0 .55 9 -0.330 -0.467 -0.623 -0.028 0.076 0.122 0.4 95 0.4 95 0.444 0 .57 0 0 .54 2 0 .54 2 0.7 65 0.2 45 0 .59 5 0.620 0 .59 3 0.0 95 0.840 -0. 053 0.786...
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Báo cáo y học: "Comment on and reply to "Analysis of variation of amplitudes in cell cycle gene expression" by Liu, Gaido and Wolfinger: On the analysis of gene expression during the normal, eukaryotic, cell cycle" docx
... Revisiting retinoblastoma protein phosphorylation during the mammalian cell cycle Cell Mol Life Sci 2001, 58 :58 0 -59 5 Cooper S: The Schaechter-Bentzon-Maaløe experiment and the analysis of cell ... amplitudes in two different experiments In Table they are trying to explain differences between different experiments in terms of variation in the phase angles of peaking of gene expression in different ... artifacts would be eliminated in the second cycle 5) Successive generations (i.e the time between rises in cell number) should be of equal length and equal to the doubling time of cells in exponential...
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Global gene expression analysis of cranial neural tubes in embryos of diabetic mice
... determines the fate of neural progenitors during neurogenesis (Shu et al., 2006) 2.2.1.2 Brain lipid binding protein (Blbp) Brain lipid binding protein (Blbp) is a member of the fatty acid-binding ... differentially expressed in embryos of diabetic pregnancy A majority of those genes including doublecortin (Dcx), doublecortin-like kinase (Dclk), brain lipid binding protein (Blbp), insulin-like growth ... arrays……………… … 55 5. 8 Data analysis …………………………………………………………… … 57 vi TABLE OF CONTENTS 5. 8.1 Absolute data analysis ……………………………………………… 57 5. 8.2 Comparison data analysis ……………………………… ………… 58 5. 8.3 Gene filtration…………………………………………………………. 58 ...
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Báo cáo y học: " Transcriptome analysis of murine thymocytes reveals age-associated changes in thymic gene expression"
... promotes Glut1 trafficking and glucose uptake via http://www.medsci.org Int J Med Sci 2009, 51 52 53 54 55 56 57 58 59 60 61 62 63 64 64 STAT5-mediated activation of Akt to support T -cell survival Blood ... lambda-1 chain C region RIKEN cDNA B230118H07 Z Ratio 16M-1M 13.81 Z Ratio 24M-1M 12.67 9.02 7.98 2.04 AF 152 371 6.89 6. 85 Mm.333124 55 S 250 58 LOC5 458 54 IgJ Igh-6 54 5 854 16069 Mm.4262 05 0.60 Mm.1192 ... request Most of the genes with the greatest increase were immunoglobulin-associated genes This was a very interesting finding, given that the number of immunoglobu- lin-producing cells within the thymus...
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A CFD analysis of transport phenomena and electrochemical reactions in a tubular-shaped PEM fuel cell
... may select a maximum operating point at 0 .56 V and 1. 25 A/cm2, resulting in 0.7 W/cm2 and an efficiency of 0. 455 with maximum temperature gradient inside the cell of 7.9 K However, one may get ... calculated according to [22]: u in ,c = ζ c I RTin ,c AMEA xO2 ,in Pc Ach 4F (39) u in ,a = ζ a I RTin ,a AMEA x H ,in Pa Ach 2F (40) ISSN 2076-28 95 (Print), ISSN 2076-2909 (Online) ©2013 International ... maximum temperature gradient inside the cell of 7.6 K However, one may get the same power output by selecting two cells, connected in series, operating at 0. 755 V and 0 .5 A/cm2 each Obviously, the...
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Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf
... Cdc25C promoter by in vivo footprinting as being protected in G0 cells The Cdc25C gene is not expressed in resting cells or in cells in G1 phase Only in G2 phase can strong transcription of the gene ... MuvB proteins Lin-37, Lin -52 , Lin -54 and chromatinassociated Lin-9 and Lin -53 ⁄ RBBP4 ⁄ RbAp48 Furthermore, A-Myb and B-Myb were present in immunoprecipitations for Lin-9, Lin-37 and Lin -54 [90,92] ... repression of genes, such as cyclin A and cyclin B1, in dividing cells [92] Furthermore, depletion of Lin-9 and B-Myb in p53) ⁄ ) MEFs caused lowered expression of cyclin A, cyclin B and Cdc2 [94] In...
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Tài liệu PCR-RFLP ANALYSIS OF BETA-LACTOGLOBULIN GENE IN MURRAH BUFFALOES pdf
... primer,2 .5 units of Taq.DNA polymerase,1X PCR buffer(10mM Tris – HCL, pH 9.0, 50 mM KCL and 1 .5 mM MgCl2), 150 mM of each dNTP and 50 ng of genomic DNA After an initial denaturation of at 950 C, 35 cycles ... pattern of betalactoglobulin in buffalo milk Tamil Nadu Journal of Veterinary and Animal Sciences, 3: 150 - 155 Meignanalakshmi,A., Mahalinga Nainar,A and Nachimuthu,K (2001) Identification of genetic ... mg/ml), 0 .5 ml of 0 .5 M EDTA,pH 8.0 and 0 .5 ml of 20% SDS were added, mixed well and incubated at 50 0C in a water bath for 3h with occasional shaking To the digested sample, ml of saturated 1 95 Amplification...
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Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
... 4 .5 1.9 4.3 1.7 4.1 1 .5 50 100 150 200 250 300 350 400 450 Time (min) 50 100 150 200 250 300 350 400 450 Time (min) Fig Immunocytochemical staining of A549 cells and analysis of the dynamics of ... 0 0 0.06 0. 05 0.04 0 0.03 0 0.02 0 0.01 0 50 100 150 250 200 Time (min) 300 350 400 450 50 100 150 200 250 300 350 400 450 Time (min) Fig Effect of SC -51 4 inhibitor on the activity of rhIKK2 homodimer ... simulated in silico Combining this method of analysis (in vitro, cell- based and in silico analysis) will facilitate systematic understanding of the underlying properties of this signaling pathway...
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Báo cáo khoa học: Genomic structure and expression analysis of the RNase j family ortholog gene in the insect Ceratitis capitata pptx
... (NC_000017:6 856 522–6 858 5 75) , Mus musculus (NC_000077:c70 053 348–70 051 628), Rattus norvegicus (NC_0 051 109), Bos taurus (NC_007317:c57078994 57 077 154 ), Canis familiaris (NC_00 658 7: 350 37037– 350 38710), ... complexity of ARE-mediated pathways is manifested by the presence of multiple ARE-binding proteins, including stabilizing proteins [29,30] or proteins that promote degradation of mRNAs that contain AREs ... finding implies the existence of RNase L, which plays an important role in the response of cells to dsRNA during events such as virus infection in insects In Ceratitis capitata, three RNA-degrading...
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ADVANCES IN QUANTITATIVE ANALYSIS OF FINANCE AND ACCOUNTING Volume 5 potx
... ADVANCES IN QUANTITATIVE ANALYSIS OF FINANCE AND Accounting June 8, 2007 3:16 spi-b483 Advances in Quantitative Analysis of Finance and Accounting: Vol .5 Advances in Quantitative Analysis of Finance ... enquiries@stallionpress.com Printed in Singapore June 28, 2007 8: 15 spi-b483 Advances in Quantitative Analysis of Finance and Accounting: Vol .5 Preface Advances in Quantitative Analysis of Finance and Accounting is ... 2007 8: 15 spi-b483 Advances in Quantitative Analysis of Finance and Accounting: Vol .5 This page intentionally left blank FM June 28, 2007 8: 15 spi-b483 Advances in Quantitative Analysis of Finance...
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Báo cáo khoa học: Functional analysis of pyrimidine biosynthesis enzymes using the anticancer drug 5-fluorouracil in Caenorhabditis elegans docx
... and their role in fluoropyrimidine sensitivity in colon cancer cell lines Int J Biochem Cell Biol 39, 56 5 57 5 10 Watanabe S & Uchida T (19 95) Cloning and expression of human uridine phosphorylase ... worms with 5 -deoxy -5- fluorouridine (5dFUR) to further examine the function of UPP-1 in the pyrimidine biosynthesis pathway 5dFUR is converted to 5- FU by UP and TP [9] The effects of 5dFUR in the ... plates following RNAi for worm homologs of various human genes F25H2 .5 is a putative homolog of uridine diphosphate kinase, and Y43C5A .5 is TK R12E2.11 and T07C4.1 are OPRT domain proteins F19B6.1,...
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Báo cáo khoa học: Cloning and functional analysis of 5¢-upstream region of the Pokemon gene pptx
... A549 and DU1 45 cells Figure 3C shows that deletion of the region from )56 0 to )54 2 resulted in a remarkable increase in luciferase activity, by 25- fold in A549 cells and 24.6-fold in DU1 45 cells ... cells and human acute T -cell leukemia cells were maintained in RPMI-1640 medium containing 10% fetal bovine serum (Invitrogen) All these cells were incubated in a humidified 5% CO2 incubator at 37 ... alone or in combination, were created in F -56 0 and transiently transfected into A549 and DU1 45 cells As shown in Fig 7A, mutation of the NEG-U element alone resulted in a significant increase in luciferase...
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Báo cáo khoa học: Gene duplication and separation of functions in aB-crystallin from zebrafish (Danio rerio) pptx
... been identified in cellular retinoic acid-binding proteins [23] Separation of functions after gene duplication also occurred during evolution of d-crystallin, a major component of the bird and ... comparative analysis of gene regulation using the two aB-crystallin genes makes the zebrafish an excellent model for examining the evolution of lens-specific expression Mechanisms behind the evolution of ... aB-crystallin to prevent the aggregation of a-lactalbumin were also examined in triplicate over the temperature range 25 40 °C at °C increments All reactions were in a total of 50 0 lL using a 5- mm...
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Báo cáo khoa học: Analysis of the contribution of changes in mRNA stability to the changes in steady-state levels of cyclin mRNA in the mammalian cell cycle doc
... fractionation of cells into cell cycle subpopulations was accomplished by increasing the pump speed stepwise from the initial flow rate of 20 mLÆmin)1 to a maximum of 55 mLÆmin)1 Cell fractions of 100 ... the cell cycle [11– 15] Certain transacting proteins such as HuR were proposed as regulators of changes in mRNA stability during the cell cycle [ 15] In this context it is of interest that during ... function during the human cell cycle Nature Genet 27, 48 54 Coqueret O (2002) Linking cyclins to transcriptional control Gene 299, 35 55 Moroy T & Geisen C (2004) Cyclin E Int J Biochem ¨ ¨ Cell Biol...
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Báo cáo khoa học: Dual expression of mouse and rat VRL-1 in the dorsal root ganglion derived cell line F-11 and biochemical analysis of VRL-1 after heterologous expression pptx
... results in a protein of 761 amino acids for rVRL-1 and of 756 amino acids for mVRL-1 which cannot be separated by SDS/PAGE The primers we chose to examine rat VRL-1 in F-11 cells were also capable of ... migrating band to the N18TG2 control (Fig 5) The existence of both rat and mouse VRL-1 in the same cell was confirmed by F-11 single cell RT-PCR In all analyzed cells (n ¼ 15) expression of both ... of rat brain, F-11 and N18TG2 cells was prepared using TRIzol Reagent (Invitrogen) according to the instruction of the manufacturer lg total RNA was incubated for 10 at 65 °C with 50 0 ng Oligo(dT)Primer...
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