... different geometrical cues The design ofthe geometry ofthe 3D scaffold has a direct implication on the pore size [71] In view ofthe importance of these two factors ofthe biopolymer structures ... interactions The first part ofthe thesis involved the use of conventional macrorheological technique to uncover the rich mechanical properties of collagen, the most abundant protein found in mammalian The ... understand the biomechanical role each of these tissues have on the cardiac cycle [77] Figure 1.8: Three basic stress encountered by soft tissues [75] The tensile and compressive strength of human tissues...
... interpret the extracted parameters 1.2.5 Consideration ofthe Substrate Effect One ofthe most popular applications of nanoindentation is the determination ofthemechanical properties of thin films The ... (1-38) where a is the contact radius; µf is the shear modulus ofthe thin film; The complex kernel function g(w) takes combinations ofthemechanical parameters ofthe film and the substrate as ... related to themechanical properties, such as the reliability of polymeric low-k films, the resistance of cells to the invasion of virus, the effect of drugs on cell membranes, and so on In the next...
... interspersed by a stimulus which deviated from the standard in the intensity ofthe first component and the glide direction ofthe second constituent The number of distinct MMN components elicited by ... 56]; these findings further support the concept ofthe P3 as a proxy for some element of stimulus evaluation time Evidence has accumulated describing the P300 as a cognitive routine supporting the ... and the N2b Data indicate that the P300 is involved not only in processes of working memory, but may interact with the N2b in the control of motor response to external cues Through the use of...
... from the anoxic tank, and the amount of sludge was adjusted to 9.4 % of total MLSS per day Ozonation was performed twice a week, and then the supernatant ofthe ozonated sludge was flowed into the ... the sewer line is lower than those in the UCT processes In sequencing batch reactors (SBRs), the PURs are higher than the others (Table 1) These high PURs were due not to the differences in the ... mg/L of SS, 55–80 mg/L of TOC, 45–55 mg/L of T-N, and 4.0–5.5 mg/L of T-P Other details are shown in the related study (Kondo et al., 2009) Sampling was performed once efficient - 156 - Journal of...
... the deciduous effect ofthe respective parts of Z japonica The δ13C in the roots and rhizomes seems to be larger than the δ13C in the leaves and stems For the same species of seagrass, when the ... (LCFAs) These standards were used to obtain 56 kinds of fatty acids Therefore, the contents of each fatty acid were calculated based on the quantity of lipid, the content ratios of all the fatty ... we think that the characteristics ofthe fatty acid structure in the leaves and stems differ from those ofthe roots and rhizomes because the content ofthe fatty acid 18:3n3 in the leaves and...
... the inner part ofthe membranes As shown in Figure 4e, many big aggregates appear in the middle part ofthe PVDF-PS membrane The hydrophobic property of PS polymer promoted the formation of these ... Thermal analysis ofthe PVDF-SPS composite membrane The TG analysis curve is presented in Figure for the investigation ofthe thermal stability ofthe PVDF membrane, PVDF-PS membrane, and the PVDF-SPS ... decomposes The swelling is due to the degradation ofthe cross-linking structure ofthe membrane in the H2O2 solution [33] The decomposition rate is larger than the swelling effect after 60 h The result...
... wavelength of 1.540600 Å The X-ray data was collected using an MBraun curved PSD with a chamber depth of cm The focal radius ofthe system was 57 mm, and the cm length ofthe PSD subtended ∼25° 2θ ofthe ... however, the assignment of component polymorphs to sample mixtures depended on the XRPD results Conclusions Interconversions ofthe Polymorphs of Disodium Salt The interconversions of polymorphs ofthe ... anhydrous thermal event Form I to Form II melt of Form II Form I to Form II melt of Form II loss of water melt of Form I Form I to Form II melt of Form II loss of water melt of Form III melt of Form...
... unclear whether these cytokines are produced by CD4+CD25+Foxp3+Treg or whether they induce the production of these cytokines from another population of cells [20] To date, our knowledge about the different ... important in the generation ofthe correct class of antibodies by B-cells, and for the elimination of extracellular pathogens, such as helminths and other extracellular parasites [2] Th17 is the most ... providing further evidence of their identity Multiple alignments ofthe zebrafish t-bet, stat6 and foxp3 amino acids with their vertebrate homologues revealed regions of high conservation These regions...
... implications ofthe observed VpUcyt structure One ofthe most studied activities of VpU is the induction of CD4 degradation in the endoplasmatic reticulum of infected CD4+ cells Only the cytoplasmic ... spectra of VpUcyt in detergent-free buffer confirmed the absence of a secondary structure (Fig 1) The presented data are the most comprehensive account ofthe lack of a conformational preference of ... downwards The vertical arrow represents the normal ofthe micelle–water interface Two faces ofthe same structure are shown They are related to each other by a 180° rotation about the normal...
... For the fraction of substrates that undergo the reaction, the resultant products dissociate from the ribozyme relatively slowly on the time scale ofthe reaction Furthermore, dissociation ofthe ... to (if not the same as) the role ofthe xG in the second step of self-splicing [4856] This suggests that the guanosine to be excised is likely binding to the GBS ofthe ribozyme for the exon-ligation ... appears that there is room for improvement in terms ofthe rate of reaction This would be benecial not so much in terms ofthe rate ofthe overall reaction, as the cleavage reaction is not the limiting...
... analyses ofthe predicted amino acid sequence showed that the C-terminal half ofthe RepA ofthe pIT3 plasmid is sequence-similar to the helicases ofthe phage-encoded superfamily III proteins The ... the two-metal-ion mechanism of both RNA and DNA synthesis [17] In a previous study, we reported the findings of an analysis ofthe complete sequence ofthe cryptic plasmid pIT3 isolated from the ... [14] The side-chains ofthe first pair of residues, i.e K135 and R148, matched almost exactly; those ofthe second pair were in close proximity The R148 residue ofthe Pfu-primase is part of a...
... and the remodelling of larval tissues to produce adult tissues (i.e the development ofthe gills and the production of an adult shell), which is accompanied by significant growth ofthe soft body ... is the mean + SE of three pools of four animals (tissues) or the mean of one pool of embryos or larva from one spawn Expression levels are related to 100 copies of GAPDH (B) Localization of Cg-Clp2 ... based on the conserved amino acid sequences ofthe catalytic domain of members ofthe GH18 family resulted in the amplification of an expected 147 bp sequence Cloning and sequencing of this fragment...
... constants ofthe 15N nucleus ofthe distal NO and ofthe 14N nucleus ofthe proximal His ofthe GmHO-1 complex are closer to those ofthe rHO-1 complex than to those ofthe SynHO-1 complex Thus, ... ofthe heme pocket of GmHO-1 might be associated with the smaller values of Kazide for GmHO-1 and SynHO-1, and with the ferric character ofthe heme The Kimidazole values ofthe three kinds of ... against the concentration of azide of each solution The intersection point corresponding to the mole fraction of 0.5 ofthe respective forms gives the amount of azide necessary to attain the equilibrium,...
... fragments F and I The number at the right of each RT-PCR product represents the length ofthe product The scheme also shows the location ofthe reverse primer DN+89 used in another starting exon-specific ... numbers over the diagrams indicate the position ofthe corresponding nucleotides relative to the translation start site, and the numbers in parentheses below the diagram show the position ofthe corresponding ... is done from +1 of exon )11871 above the sequence indicates the 5¢-end of exon 1a The numbers in parentheses demonstrate the position ofthe corresponding nucleotides relative to the transcription...
... peptides covered 39.1% ofthe sequence The molecular masses ofthe tryptic peptides, either from the mass fingerprint or purified peptides, covered 60.3% ofthe sequence, and the masses of cyanogen bromide ... which these states are alternated, has been proposed [16] The met state is the resting state ofthe enzyme, and in the absence of substrate about 85–90% ofthe enzyme is in this state [16] Both the ... changes the redox potential ofthe phenolic substrates, making them more easily oxidized Therefore, the pH profile reflects not only the optimal behavior ofthe enzyme, but also changes in the substrate...
... Results The C-terminus of isoform ofthe PM H+-ATPase of A thaliana (AHA1) interacts with the first 88 amino acids of PPI1 [22], indicating that the PM H+-ATPase binding site of PPI1 is localized therein ... downstream ofthe first 88 amino acids of PPI1 may participate in the interaction with the H+-ATPase and makes PPI1588His6 a suitable tool to study the mechanism of action of PPI1 The analysis ofthe ... PPI1588His6 The analysis ofthe effect of PPI1588His6 on the dependence of PM H+-ATPase activity on the concentration of MgATP (Fig 3) showed that stimulation decreased with the increase of PPI1588His6...
... Presumably, their homologues SNO2, SNO3, SNZ2 and SNZ3 complement the defect The relationship of all of these genes remains to be clarified Expression and purification of Sno1p and Snz1p In light ofthe ... by the complex of Sno1p with a His-tag and Snz1p The reaction was carried out in mL of 50 mM Tris/HCl, pH 8.0, in the presence of 1–10 mM glutamine and 50 lg ofthe complex, at 30 °C for 10 The ... feature ofthe genes SNO1 and SNZ1 is that they act simultaneously in the stationary phase of yeast cells [8] They are assumed to participate in the metabolism of nucleotides In this context, the...
... value of LMWC is due to the release of GlcNAc and GlcNAc-rich oligomers as some ofthe products An examination of DA values indicates that the total DA ofthe products is equal to that of native ... the ordered structure This is probably due to the decrease in the DA (Table 1) of LMWC, which results in lower degree of hydration ofthe molecule affecting the crystallinity index and thus the ... depends on the conformation ofthe primary -OH group (C6) in a favorable orientation [33] The shift ofthe same to 1458 cm)1 in the LMWC would probably indicate a change in the environment of primary...
... (enzyme I ofthe PTS), the rst protein in the cascade of proteins forming the PTS, to HPr, the histidine-phosphocarrier protein HPr is the smallest protein in the protein cascade ofthe PTS and ... determine the cold-denaturation at this urea concentration were unsuccessful, probably because ofthe absence of enough data in the cold-denaturation region ofthe curve (Fig 5B) b The values ofthe thermodynamic ... slope ofthe observable change with the temperature; and, the last term is the eect of urea on the baselines To allow for comparisons among the thermal- where m, mDHi , mDSi and mDCpi are the changes...
... cells The positions ofthe molecular size markers (kDa) are indicated to the left and right ofthe (C) and (D), respectively, whilst the position ofthe Oct-1 (98 kDa approximately) and the two ... regions of Prm3 The following lists the identities ofthe Prm3 gene fragments and corresponding plasmids generated in either pGL3Basic (pGL3b) or pGL3Enhancer (pGL3e) vectors and the identity ofthe ... the regulation of basal Prm3 activity It is anticipated that the functional characterizationof Prm3 reported herein should provide critical knowledge ofthe modes of regulation of TPb expression...