2classification using fatty acid and phenolic compound profiles established by direct infusion ms

THÀNH PHẦN hợp CHẤT AXÍT béo và CAROTENOID của gấc fatty acid and carotenoid composition of gac (momordica cochinchinensisspreng) fruit

Ngày tải lên : 09/02/2014, 00:08

... Fatty Acid and Carotenoids in Gac Fruit MATERIALS AND METHODS Fatty Acid Analysis Materials Gac fruit were purchased from two Asian markets (Vinh Phat and Shun Fat) in Sacramento, ... relative standard deviation, % vaccenic (in two fruit), R-linolenic, and eicosa-11-eneoic acids were also detected The fatty acid composition of the aril and seed are interesting, and they reflect ... Hilditch and Williams (27), analogous to the pulp surrounding the seed in avocado, olive, and palm The principal fatty acid components of such fats are palmitic, oleic, and linoleic acids Gac...

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Using biological networks and gene expression profiles for the analysis of diseases

Ngày tải lên : 09/09/2015, 10:15

... microarray platforms but is also able to handle small-size training samples This enables researchers to find the mechanisms behind a disease and use them directly as a tool for diagnosis and prognosis ... aggravated by the lack of samples because many laboratories are constrained by budget, biology or other factors; making it hard to draw reasonable and consistent biological conclusions By using databases ... (2.6) where x1 and x2 denote the mean gene-expression value for the two classes respectively and se refers to the standard error of the difference between two means and has different forms depending...

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Báo cáo Y học: Concerted regulation of free arachidonic acid and hormone-induced steroid synthesis by acyl-CoA thioesterases and acyl-CoA synthetases in adrenal cells pdf

Ngày tải lên : 08/03/2014, 09:20

... four isoforms with different subcellular locations and a high degree of homology [35] The family includes a cytosolic (CTE-I), a mitochondrial (MTE-I) and peroxisomal forms (PTE-Ia and Ib) of ... purified enzyme resulted in the release of AA Km and Vmax values obtained for the reactions were 4.1 and lM and 948 and 193 nmolÆmin)1Æmg)1 for CTE-I and a MTE-I, respectively Next, we measured the ... by the dotted line The effect of NDGA was highly significant (P < 0.001) by ANOVA, and indicated values (*) significantly different (P < 0.05) by Student-Newman-Keuls post test triacsin C, thereby...

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Báo cáo y học: "Gene expression profiling in circulating endothelial cells from systemic sclerosis patients shows an altered control of apoptosis and angiogenesis that is modified by iloprost infusion" potx

Ngày tải lên : 12/08/2014, 14:22

... CECs and EPCs levels between healthy controls and patients affected by SSc with and without ulcers were performed by T-test and Pearson test Correlations between CECs and EPCs number before and ... Verona) and informed written consent was obtained from all the participants to the study Detection of circulating endothelial cells and progenitors by flow-cytometry CECs and EPCs were directly ... array analysis Cell pellets of CECs and EPCs obtained from SSc patients, with and without digital ulcers, before and 72 hours after iloprost infusion both after one and five days of therapy (test...

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báo cáo khoa học: "Fatty acid profiles and their distribution patterns in microalgae: a comprehensive analysis of more than 2000 strains from the SAG culture collection" docx

Ngày tải lên : 11/08/2014, 11:21

... α-linolenic acid; ARA: Arachidonic acid; CVA: canonical variance analysis; DHA: docosahexaenoic acid; DMOX: 4, 4-dimethyloxaline; EPA: Eicosapentaenoic acid; FA: fatty acid; FAME: fatty acid methyl ... identified by mass spectrometry, 76 of which represent methyl esters of FAs Out of the 76 fatty acids, 36 substances were identified by their mass spectrum and by retention time according to a standard ... Trebouxiophyceae (arrowheads) and Ulvophyceae (circles) as based on their fatty acid distribution patterns using Canonical Variates Analysis Both vectors correspond to variables (fatty acids) correlated...

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Effect of group housing and exercise on brown fat gene expression and fatty acid profiles

Ngày tải lên : 21/09/2015, 21:25

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Characterization of the Carbon Stable Isotope Ratio and Fatty Acid Structure of Zostera japonica in Coastal Areas

Ngày tải lên : 05/09/2013, 10:17

... organisms, such as bacteria, periphyton and sessile animals We define the fatty acids with > 5% content as dominant fatty acids and Fig shows the dominant fatty acids in the leaves and stems of ... japonica We found about 30 kinds of fatty acids in the leaves and stems and about 36 kinds of fatty acids in the roots and rhizomes of Z japonica We focused on the fatty acid content of Z japonica , ... Fig.4 Dominant fatty acid content in the roots the Fig - Dominant fatty acid content in and stems at each sampling site．sampling and rhizomesand rhizomes site． each leaves and stems at each roots...

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Effect of unsaturated fatty acid esters of biodiesel fuels on combustion, performance and emission characteristics of a DI diesel engine

Ngày tải lên : 05/09/2013, 15:28

... increases the risk of polymerization and acidiﬁcation and of the appearance of insoluble sediments and gums, which can lead to ﬁlter plugging and deposits in the fuel systems Esterified soybean oil was ... other hand, the need for standardization of biofuels physical and chemical properties has been widely recognized In fact, it is widely recognized that only the existence of standards and norms may ... of Energy and Environment (IJEE), Volume 1, Issue 3, 2010, pp.411-430 427 properties and fatty acid composition on CO and HC emissions The correlation analysis reveals that the CO and HC emissions...

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Photodegradation and removal of phenol and phenolic derivatives from petroleum refinery wastewater using nanoparticles of TiO2

Ngày tải lên : 05/09/2013, 17:03

... Energy and Environment (IJEE), Volume 3, Issue 2, 2012, pp.267-274 273 Conclusion The degradation of the phenol and phenolic compounds using a circulating and direct irradiation reactor seems to ... compares the chromatograms of phenolic compounds before and after treatment by the photocatalyst Figure Comparison of chromatograms for degradation of phenol & M-tetrabutyl phenol compounds in petroleum ... solution by using carbonised beet pulp J Hazard Mater 2005,125, 175–182 [10] Gurses A., Yalcin M Removal of phenolic and lignin compounds from bleached kraft mill effluent by fly ash and sepiolite...

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Tài liệu Báo cáo khoa học: Fatty acid synthesis Role of active site histidines and lysine in Cys-His-His-type b-ketoacyl-acyl carrier protein synthases ppt

Ngày tải lên : 19/02/2014, 08:20

... cation ligands are main-chain oxygen atoms, and three are the side-chain oxygen atoms of N296, E342 and S387 The glutamic acid and asparagine residues are conserved among known KAS I and KAS II ... mutated KAS I structures ˚ The distance between H333 Ne and C163 Sc is 3.2 A ˚ in the native and fatty acid complex, and 3.1 A respectively, and infers that H333 Ne donates a hydrogen bond to the ... two basic mutants, K328H and K328R, and the bulky mutant, K328F, evinced some activity (Fig 7A, lanes 7–12, and Fig 7B, lanes 2–4) By comparison, the acidic mutant K328E and to a lesser extent...

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Báo cáo khoa học: Spatio-temporal distribution of fatty acid-binding protein 6 (fabp6) gene transcripts in the developing and adult zebraﬁsh (Danio rerio) pptx

Ngày tải lên : 07/03/2014, 06:20

... protection of enzymes and membranes from the detergent effects of excess fatty acids by sequestering them, and (d) modulation of cell growth and differentiation by transport of fatty acids to the nucleus ... livertype fatty acid- binding protein (L-FABP), brain-type fatty acid- binding protein (B-FABP), intestinal-type fatty acid- binding protein (I-FABP), etc However, this nomenclature has become confusing ... (1972) A binding protein for fatty acids in cytosol of intestinal mucosa, liver, myocardium, and other tissues Science 177, 56–58 Haunerland NH & Spener F (2004) Fatty acid- binding proteins – insights...

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Báo cáo khoa học: Escherichia coli cyclopropane fatty acid synthase Mechanistic and site-directed mutagenetic studies potx

Ngày tải lên : 07/03/2014, 16:20

... reported by Cronan and coworkers [9], we found that CFAS could be inactivated by thiol-directed reagents such as DTNB and N-ethylmaleimide (NEM) Kinetic analysis of the inactivation process by DTNB ... Cyclopropane fatty acid synthase from Escherichia coli Enzyme purication and inhibition by vinyluorine and epoxide-containing substrate analogues Chembiochem 4, 13521356 Bradford, M.M (1976) A rapid and ... 100 lL The reaction was initiated by addition of the enzyme and incubated at 37 C for 20 The reaction was stopped by adding mL 10% (v/v) trichloroacetic acid, and the solution was ltered over...

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Báo cáo khoa học: Differential tissue-speciﬁc distribution of transcripts for the duplicated fatty acid-binding protein 10 (fabp10) genes in embryos, larvae and adult zebraﬁsh (Danio rerio) docx

Ngày tải lên : 16/03/2014, 00:20

... uptake and utilization of fatty acids, intracellular targeting of fatty acids to speciﬁc organelles and metabolic pathways, and the protection of cellular structures from the detergent effects of fatty ... (1993) Fatty acidbinding protein and its relation to fatty acid oxidation Mol Cell Biochem 123, 101–106 15 Glatz JF & van der Vusse GJ (1996) Cellular fatty acidbinding proteins: their function and ... JH & Hsu KT (2002) Similar mechanisms of fatty acid transfer from human and rodent fatty acid- binding proteins to membranes: liver, intestine, heart muscle and adipose tissue FABPs Mol Cell Biochem...

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Báo cáo khoa học: Fatty acid composition of chylomicron remnant-like particles inﬂuences their uptake and induction of lipid accumulation in macrophages pot

Ngày tải lên : 16/03/2014, 12:20

... Table Fatty acid composition of TG in CRLPs CRLPs were prepared using TG from palm, olive, corn or ﬁsh oil as described in Experimental procedures, and the fatty acid composition was determined by ... SFAs, MUFAs, n)6 PUFAs and n)3 PUFAs [23], as well as containing a range of other fatty acids, and these enrichments inﬂuence the uptake and metabolism of the particles by the liver [24–26,31] ... the particular type of fatty acid predominating in the oil, but also contained a complex mixture of other fatty acids, as occurs in vivo As might be expected, the fatty acid composition of CRLPs...

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Báo cáo khoa học: Two novel Mesocestoides vogae fatty acid binding proteins – functional and evolutionary implications doc

Ngày tải lên : 23/03/2014, 07:20

... ﬂuorescent fatty acid transfer from adipocyte and heart fatty acid binding proteins by acceptor membrane lipid composition and structure J Biol Chem 269, 10517–10523 47 Hsu KT & Storch J (1996) Fatty acid ... Veerkamp JH, van Kuppevelt THMSM, Maatman RGHJ & Prinsen CFM (1993) Structural and functional aspects of cytosolic fatty- acid binding proteins Prostaglandins Leukot Essent Fatty Acids 49, 887–906 FEBS ... putative ligands of MvFABPs, their equilibrium dissociation constants and pH dependence, 3D structure, subcellular localization, and the mechanism of fatty acid transfer between FABPs and phospholipid...

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Báo cáo khoa học: Characterization of a hemocyte intracellular fatty acid-binding protein from crayﬁsh (Pacifastacus leniusculus) and shrimp (Penaeus monodon) pdf

Ngày tải lên : 23/03/2014, 10:21

... Vertebrate FABPs are involved in cellular fatty acid transport and utilization and compartmentalization of intracellular fatty acid storage, and also in fatty acidinduced regulation of gene expression ... similarity in the ligand-binding pocket to bovine and human CRABP [5] However, recent ligand-binding studies with recombinant msCRABP showed high afﬁnity binding for fatty acids and negligible interaction ... Berk [38] Brieﬂy, the fatty acids (Sigma) were dissolved at 50 lm in 0.1 m NaOH at 37 °C, and a solution of 2.5 mm fatty acid- free BSA (Sigma) in NaCl ⁄ Pi was added to a fatty acid ⁄ albumin molar...

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Báo cáo khóa học: Genetic defects in fatty acid b-oxidation and acyl-CoA dehydrogenases Molecular pathogenesis and genotype–phenotype relationships ppt

Ngày tải lên : 23/03/2014, 12:20

... Genetic defects in fatty acid oxidation (Eur J Biochem 271) 471 Table Transporter proteins and enzymes involved in the mitochondrial saturated fatty acid oxidation FATP, fatty acid transport protein; ... the fatty acid oxidation deficiencies, may be assessed Genotype–phenotype relation in fatty acid oxidation deficiencies A second level of analysis of the genotype–phenotype relation in fatty acid ... acids [27] Not surprisingly, the severe metabolic block results in profound energy deficiency and corresponding severe clinical symptoms To what degree the accumulated long-chain fatty acids and...

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Báo cáo khoa học: Differential regulation of fatty acid amide hydrolase promoter in human immune cells and neuronal cells by leptin and progesterone pdf

Ngày tải lên : 30/03/2014, 15:20

... [28], and equal loading of extracts was veriﬁed by Ponceau staining [13] Cytosolic levels of phospho-STAT3 and nuclear levels of total Ikaros isoforms were quantiﬁed by ELISA, performed by coating ... (2002) Endocannabinoids in the immune system and cancer Prostaglandins Leukot Essent Fatty Acids 66, 319–332 24 Cravatt, B.F & Lichtman, A.H (2003) Fatty acid amide hydrolase: an emerging therapeutic ... Francke, U (1998) Conserved chromosomal location and genomic structure of human and mouse fatty- acid amide hydrolase genes and evaluation of clasper as a candidate neurological mutation Genomics 54,...

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Báo cáo khoa học: Structure, mRNA expression and linkage mapping of the brain-type fatty acid-binding protein gene (fabp7 ) from zebraﬁsh (Danio rerio) potx

Ngày tải lên : 31/03/2014, 07:20

... percentage amino acid identity between zebrafish and human, mouse and pufferfish B-FABP is 83%, 76% and 83%, respectively The percentage amino acid identity between zebrafish and human and mouse is ... amino acid sequence encoded by each exon of the zebrafish B-FABP gene (ZFb-FABP) was aligned with the amino acid sequence encoded by each exon from the human (HMb-FABP), mouse (MSb-FABP) and pufferfish ... testes and between 50 and 160 times higher in brain than in muscle, intestine and heart No product was generated by qRT-PCR from liver, ovary, skin and kidney RNA Both conventional RT-PCR and qRT-PCR...

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Báo cáo khoa học: Structure, linkage mapping and expression of the heart-type fatty acid-binding protein gene (fabp3 ) from zebraﬁsh (Danio rerio) pot

Ngày tải lên : 31/03/2014, 07:20

... of fatty acids within the oocyte The accumulation of fatty acids may be sequestered by FABP to prevent cytotoxicity to the cell, and transported by FABP to sites of triglyceride synthesis and/ or ... transportation and utilization of fatty acids in cardiac muscle [4,5] However, H-FABP is also abundant in tissues that not use fatty acids as an energy source such as mammary gland and developing ... inhibitor/heart -fatty acid- binding protein Gene 147, 237–242 10 Prinsen, C.F & Veerkamp, J.H (1996) Fatty acid binding and conformational stability of mutants of human muscle fatty acidbinding protein...

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