2 phenotype changes of fut8 knockout mouse core fucosylation is crucial for the function of growth factor receptor s
Báo cáo y học: " γ δ Vγ9/Vδ2 T lymphocytes in Italian patients with Behçet’s disease – evidence for expansion, and tumour necrosis factor receptor II and interleukin-12 receptor β1 expression in active disease" pptx
... Behçet s disease [24 ], were studied The activity of Behçet s disease was assessed by the 1994 criteria for disease activity of Behçet s disease, proposed by the Behçet s Disease Research Committee of ... (Sigma) Statistical analysis Student s t-test was used to compare responses in different groups P < 0.05 was chosen for rejection of the null hypothesis Results γ δ Expression of Vγ9/V 2 T-cell receptor ... this time the percentage of expansion was assessed by fluorescence activated cell sorting (FACS) analysis using the anti-TCR V 2 mAb The results were expressed as EF (see Materials and methods)...
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Báo cáo y học: "The matrix-forming phenotype of cultured human meniscus cells is enhanced after culture with fibroblast growth factor 2 and is further stimulated by hypoxia" pdf
... capacity of meniscus cells to re-express the matrix-forming phenotype of meniscus cells Comparison of these expression levels with cells in tissue showed that COL1A2 expression in FGF2-derived ... for P1 and P2 meniscus cells in the presence and absence of FGF2 Results are expressed as means ± SD (n = 4) FGF2, fibroblast growth factor 2; P1, passage 1; P2, passage meniscus cells with or ... experiments included in this manuscript WSK was responsible for tissue procurement and processing TEH supervised and oversaw the completion of the studies as well as the writing of this manuscript...
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báo cáo khoa học: "Sustained complete remission of human epidermal growth factor receptor 2-positive metastatic breast cancer in the liver during long-term trastuzumab (Herceptin) maintenance therapy in a woman: a case report" docx
... remission in these cases ranges from four months to eight years, and in all cases, maintenance therapy was based on trastuzumab One of these cases also illustrates the risk of withdrawing trastuzumab ... breast cancer with trastuzumab beyond disease progression J Clin Oncol 20 04, 22 :1063-1070 Fountzilas G, Razis E, Tsavdaridis D, et al: Continuation of trastuzumab beyond disease progression is ... Support for the importance of maintaining HER2 suppression is also provided by studies evaluating the use of trastuzumab beyond progression Accumulating evidence shows the benefits of trastuzumab...
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Báo cáo y học: " PDZ domain-binding motif of human T-cell leukemia virus type 1 Tax oncoprotein is essential for the interleukin 2 independent growth induction of a T-cell line" ppsx
... discs large tumor suppressor [33-36] These results present an attractive hypothesis that PBM is a factor responsible for the distinct pathogenic activities of HTLV-1 and HTLV2 Since HTLV-1 is ... Tax2B Tax2B+C Figure PBM is essential for outgrowth of CTLL -2/ Tax cells in the absence of IL -2 PBM is essential for outgrowth of CTLL -2/ Tax cells in the absence of IL -2 (A, B) CTLL -2 cells (107) ... but not the subsequent rate of growth Further analysis is required to solve this interesting question Several tumor viruses have both high-risk and low-risk subtypes High-risk viruses induce...
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Tài liệu Báo cáo khoa học: Mitogen-activated protein kinase phosphatase-1 modulated JNK activation is critical for apoptosis induced by inhibitor of epidermal growth factor receptor-tyrosine kinase pdf
... c-Abl tyrosine kinase in the stress response to DNA-damaging agents Nature 376, 785–788 22 Davis RJ (20 00) Signal transduction by the JNK group of MAP kinases Cell 103, 23 9 25 2 23 Chang NS (20 01) ... MM, Basso A, Averbuch SD & Rosen N (20 01) The tyrosine kinase inhibitor ZD1839 (‘Iressa’) inhibits HER2-driven signaling and suppresses the growth of HER2-overexpressing tumor cells Cancer Res 61, ... mitochondria Some reports have shown that the activation of the Fas ⁄ FasL system may be one of the mechanisms responsible for drug-induced apoptosis in a variety of JNK activation is critical for AG1478-induced...
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Tài liệu Báo cáo khoa học: Receptor binding characteristics of the endocrine disruptor bisphenol A for the human nuclear estrogen-related receptor c pptx
... activity, and the inverse antagonist activity of BPA (1 and 10 lM) against the inverse agonist activity of 4-OHT (1 and 10 lM) The assay set marked with an asterisk shows the the inverse antagonist activity ... Because Ala lacks the characteristic side chains of Glu and Arg, the mutant receptors are devoid of the functional groups at the particular positions of 27 5 and 316 Thus, it becomes difficult for them ... on Risk of Chemical Substances from the Ministry of Health, Labor and Welfare of Japan This work was also supported in part by grants-in-aid from the Ministry of Education, Science, Sports and...
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Tài liệu Báo cáo khoa học: Cell surface heparan sulfate proteoglycans Target and partners of the basic ®broblast growth factor in rat Sertoli cells pptx
... decrease (about )30%) of FSH-stimulated estradiol synthesis Nevertheless, this decrease was lesser by comparison to the one observed in the presence of FSH and bFGF ()49%) This result suggested ... towards understanding, a recent demonstration indicated that the synthesis of cell surface HSPG and FSH-stimulated estradiol synthesis are inversely correlated This suggests a potential role for these ... concomitent loss of steroidogenic response to FSH These data and our results suggest that bFGF could modulate, in part, the decrease of FSH-stimulated estradiol synthesis via HSPG REFERENCES Nishimura,...
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Tài liệu Báo cáo Y học: The insert within the catalytic domain of tripeptidyl-peptidase II is important for the formation of the active complex potx
... Identification of the region causing different association characteristics The difference in association characteristics of the enzyme from the two sources was surprising because the sequence is extremely ... to the large insertion between the catalytic Asp and His in TPP II This region would, of course, be difficult to model, but as Arg2 52 is so close to His264, where the structure is conserved, the ... versa were constructed by use of an XmnI site When stable transformants expressing these chimeric constructs were studied, it was evident that the sequence difference responsible for the lack of...
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Báo cáo khoa học: Calmodulin-mediated regulation of the epidermal growth factor receptor doc
... both phospholipases Cc and A2, together with the subsequent synthesis of a series of messengers that act as effectors of Ca2+-channels, is responsible for the cytosolic Ca2+ rise Phospholipase Cc ... pathogenesis [16–19] The Ca2+ signal generated by the EGFR The activation of the EGFR generates a Ca2+ signal, broadly defined as the transient rise of the intracellular concentration of Ca2+ This is ... CaM-dependent systems because of the possible existence of unwanted side effects as a result of the potential detachment of autoinhibitory sites of the protein under study from cell membranes In this context,...
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Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx
... [38,39] Another example is the type transferrin receptor, which has N-glycosylation sites at residues Asn251, Asn317 and Asn 727 Mutation of Asn 727 decreases cell-surface expression, whereas mutation ... this way, and despite the overall importance of glycosylation, our results nevertheless argue against the existence of a single glycosylation site that is of such absolute importance that it is ... quantities of b2M (data not shown) In experiments to compare the status of our glycosylation mutants in this assay we observed that the triple mutant displayed a phenotype indistinguishable from the...
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Báo cáo khoa học: Epidermal growth factor receptor-regulated miR-125a-5p – a metastatic inhibitor of lung cancer potx
... metastasis We first performed Transwell cell migration assays PC9 cells were selected as a model system with which to assess the function of miR 125 a-5p, because they expressed endogenous miR- 125 a5p ... or typeset Technical support issues arising from supporting information (other than missing files) should be addressed to the authors FEBS Journal 27 6 (20 09) 5571–5578 ª 20 09 The Authors Journal ... suppressor Our results further substantiated the role of miRNA in tumorigenesis, and revealed the possibility of using miRNAs as potential therapeutic targets to specifically suppress oncogenic signaling...
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Báo cáo khoa học: YidC is required for the assembly of the MscL homopentameric pore potx
... membrane insertase for subunits of cytochrome bc1 oxidase and ATP synthase complexes [9] In this study, we have analysed the biogenesis of MscL using in vivo insertion and assembly assays MscL is an ... various aspects of membrane protein biogenesis because it is small and, after membrane insertion, assembles into a pentameric complex for which the structure is known [11, 12] This allows analysis of ... have a significant impact on the level of the MscL–HA complex, suggesting that the Sec translocon is dispensable for the oligomerization of the MscL subunits Discussion We have analysed the requirements...
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Báo cáo khoa học: The tetralogy of Fallot-associated G274D mutation impairs folding of the second epidermal growth factor repeat in Jagged-1 potx
... exons, it appears that the segment comprising the N-terminal domain, the DSL and the first two EGFs is most sensitive to missense mutations (Figure S1 and Tables S4 ) This is consistent with the DSL ... Plot of missense mutations found in JAG1 and associated with Alagille syndrome Table S1 List of EGF-containing proteins and the names of the mutation-associated diseases Table S2 List of disease-associated ... that is presented at the cell surface is correctly folded The results obtained in the present study suggest the opposite There are several lines of evidence in support of this hypothesis: the...
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Báo cáo khoa học: A characteristic Glu17 residue of pig carnitine palmitoyltransferase 1 is responsible for the low Km for carnitine and the low sensitivity to malonyl-CoA inhibition of the enzyme docx
... malonyl-CoA sensitivity J Biol Chem 28 1, 329 46– 329 52 22 Shi J, Zhu H, Arvidson DN & Woldegiorgis G (20 00) The first 28 N-terminal amino acid residues of human 21 8 23 24 25 26 27 28 29 30 31 32 heart muscle ... palmitoyltransferases I and II expressed in the yeast Pichia pastoris Biochemistry 36, 528 5– 529 2 Zhu H, Shi J, de Vries Y, Arvidson DN, Cregg JM & Woldegiorgis G (1997) Functional studies of yeastexpressed ... muscle isoforms of ovine carnitine palmitoyltransferase 1: residues within the N-terminus of the muscle isoform influence the kinetic properties of the enzyme Biochem J 3 72, 871–879 Zhu H, Shi...
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Báo cáo khoa học: Phosphorylation of Hrs downstream of the epidermal growth factor receptor docx
... indicates that Hrs is phosphorylated on multiple tyrosine residues upon EGF stimulation, is consistent with this view There are 29 tyrosines in Hrs, and four of these are conserved in Hrs from ... colocalizes with Src (this study), are consistent with our finding that Hrs is a substrate for Src phosphorylation It is evident, however, that kinases other than Src, Yes and Fyn may also have Hrs as ... expressing active Src were starved for h before lysis and immunoprecipitation of Hrs as described above Hrs was eluted in sample buffer for SDS/PAGE peGFP-EGFR was expressed in SYF -2 cells and...
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Báo cáo khoa học: Direct CIII–HflB interaction is responsible for the inhibition of the HflB (FtsH)-mediated proteolysis of Escherichia coli r32 by kCIII docx
... HflB also acts on several membrane-associated substrates, for which the mechanism of proteolysis is probably somewhat different [21 ] Whether kCIII would act as an inhibitor for such substrates (e.g ... Evidence for an active role of the DnaK chap- 47 72 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 erone system in the degradation of r 32 FEBS Lett 478, 27 1 27 5 Tomoyasu T, Ogura T, Tatsuta T & ... Proc Natl Acad Sci USA 103, 3066–3071 Grossman AD, Straus DB, Walter WA & Gross CA (1987) Sigma 32 synthesis can regulate the synthesis of heat shock proteins in Escherichia coli Genes Dev 1, 179–184...
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Báo cáo khoa học: Sp3 transcription factor is crucial for transcriptional activation of the human NOX4 gene doc
... plasmid suppressed the expression of Sp1 and the antiSp3 shRNA-expression plasmid suppressed the expression of Sp3 The promoter activity of the NOX4 gene was significantly reduced by cotransfection ... expressed as the mean ± standard error Statistical analysis was performed using the Student s t-test Acknowledgements This work was supported in part by a Grant-in-Aid for Young Scientists (B) 21 790 525 ... control shRNA insert, supplied by System Biosciences, was also inserted Efficiency of the shRNAexpression plasmids was verified by infection of SH-SY5Y cells and subsequent western blot analyses shRNA-expression...
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Báo cáo khoa học: PSI1 is responsible for the stearic acid enrichment that is characteristic of phosphatidylinositol in yeast pdf
... any specific decrease in the de novo synthesis of a given phospholipid, including PI Hence, the rate of synthesis and the amount of CDP-DAG molecules that were used as substrate for PI synthesis ... [33], involves the synthesis of two kinds of CDP-DAG molecules: the first type containing stearic acid at the sn-1 position would be the substrate of the sole PI synthase and the second type would ... the concentration of the corresponding FAMES was determined as described above The sn -2- acyl-1-lysoPI was immediately used for acyltransferase assays Acyltransferase activity assays G3PAT assays...
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Báo cáo khoa học: Mutation of epidermal growth factor receptor is associated with MIG6 expression docx
... or typeset Technical support issues arising from supporting information (other than missing files) should be addressed to the authors FEBS Journal 27 6 (20 09) 523 9– 525 1 ª 20 09 The Authors Journal ... (http://www.ncbi.nlm.nih.gov/geo/; accession number = GSE5 720 ) The results of the analysis surprisingly showed that ERRFI1 expression levels varied among all cell lines and no tissue-specific trend was observed (Fig 5A), ... EGF + Iressa, respectively Ic represents the summation of log2-transformed fold EGFR mutation and MIG6 expression changes caused by Iressa Therefore, decreased and increased expression was expected...
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Báo cáo khoa học: " Assessment of Epidermal Growth Factor Receptor (EGFR) expression in human meningioma" docx
... converted to the IHS by multiplying the SI scores by SP scores Statistical Analysis Prior to performing the analyses, the IHS of the specimens with more than slide cut from the specimen block were ... 1995 and 20 01 Of these, 85 were used from 85 patients for the final analysis and chosen for further study based on adequacy of tissue, tissue preservation, and unequivocal diagnostic features After ... one score, so that each of the eighty five specimens was represented only once in the data analysis Analyses of association of pathology according to WHO 20 00 classification with SI, SP, and IHS...
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