1β and il 6 production in the central nervous system by brachial plexus avulsion

Báo cáo y học: " Functional cooperation of of IL-1b and RGS4 in the brachial plexus avulsion mediated brain reorganizatio" pps

Ngày tải lên : 10/08/2014, 10:20

... Kinetic changes in IL- 1b and IL- 6 expression in brainstem after brachial plexus avulsion Mice were divided into groups, control, 1, and month of brachial plexus avulsion (n = 5) IL- 1b and IL- 6 ... upregulated genes IL- 1b and IL- 6 production in the central nervous system by brachial plexus avulsion Constitutive expression of IL- 1b and IL- 6 in brain is quite low in basal condition [18] Based ... analysis of IL- 1b and IL- 6 production IL- 1b and IL- 6 production in brainstem (50 mg) were assessed by sandwich ELISA according to the manufacturer’s instructions (R&D Systems) A 96- well plate...

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Báo cáo y học: " Quantitative biomarker analysis of synovial gene expression by real-time PCR" potx

Ngày tải lên : 09/08/2014, 01:23

... expression For instance, a CE of 1 .6 for IL -1β versus a CE of 11,2 36 for IL- 6 does not imply that more IL- 6 mRNA is present relative to IL- 1 in the tissue Instead, it relates to the expression in the synovium ... biopsies* 1 .6 0.2 15 11,2 36 22 76 20 TNF-α 81.7 ± 16. 5 56. 2 ± 9.7 5.2 ± 1 .6 4487 1074 24 IL- 6 71.7 ± 17.2 62 .7 ± 13.8 7.2 ± 1.8 MMP-1 59.4 ± 18.3 56. 4 ± 14.8 6. 4 ± 2.1 IL -1β 65 .3 ± 15.1 46. 9 ± 7 .6 4.0 ... presents the CEs of GAPDH, IL -1β, IL- 6 and MMP-1 with the standard deviation and percentage CV obtained from the replicate experiments The CE values are relative to the PBMC standards and not...

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Báo cáo khoa học: "Comparative evaluation of phenobarbital-induced CYP3A and CYP2H1 gene expression by quantitative RT-PCR in Bantam, Bantamized White Leghorn and White Leghorn chicks" ppsx

Ngày tải lên : 07/08/2014, 18:21

... 024.0 763 .1 718.0 982.1 1 76. 0 344.1 nitcA-β 319.0 157.1 1H2PYC puorG lortnoC 028.0 115.1 549.0 743.1 62 8.0 1 76. 1 A3PYC 519.0 167 .1 139.0 719.0 64 6.0 085.1 nitcA-β puorg detaert 000.0 268 .0 549.0 ... 59.9 78.03 10.03 23.82 58 .60 nitca-β matnaB 83.30 70.30 80.10 47.0 85.80 39.70 35.50 65 .30 1H2PYC 73.41 09.21 70.90 29.2 18.92 91.82 43.42 47 .60 A3 PYC 14 63 13 62 14 63 13 62 elcyC lortnoC tnemtaerT ... 59.20 52.3 68 .23 33.03 40.02 40.51 A3 PYC 00.12 49.12 52 .61 59.9 86. 40 23.50 75.50 04.20 nitca-β nrohgeL etihW 27.20 76. 10 95.00 11.10 08.00 80.10 1H2PYC dezimatnaB 43.41 14.21 33 .60 91.2 62 .90 58.21...

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Báo cáo sinh học: " Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey" potx

Ngày tải lên : 19/06/2014, 08:20

... Surprisingly probe binding and Tm appear largely unaffected by these changes (Figure 1) Of the samples confirmed by Rotorgene, of these had no mutations in the probe binding area The remaining samples ... attending a GUM clinic in The Gambia The possible role of other co-infections in the secretion of HSV in CVL fluid and HSV transmission were investigated Results Study subjects Seventy subjects included ... relative binding In a single step multiplex assay mutations in the probe binding area are reported to lead to a loss in sensitivity and error in the classification of samples [24] When diluted amplified...

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báo cáo hóa học:" Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey" pdf

Ngày tải lên : 20/06/2014, 04:20

... Surprisingly probe binding and Tm appear largely unaffected by these changes (Figure 1) Of the samples confirmed by Rotorgene, of these had no mutations in the probe binding area The remaining samples ... attending a GUM clinic in The Gambia The possible role of other co-infections in the secretion of HSV in CVL fluid and HSV transmission were investigated Results Study subjects Seventy subjects included ... relative binding In a single step multiplex assay mutations in the probe binding area are reported to lead to a loss in sensitivity and error in the classification of samples [24] When diluted amplified...

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Báo cáo y học: " Detection and quantitation of HPV in genital and oral tissues and fluids by real time PCR" pot

Ngày tải lên : 12/08/2014, 04:20

... determined by nested PCR using MY09/11 and GP5+ /6+ primers pairs followed by direct sequencing of the PCR products Homology of the PCR product with HPV 16 is indicated by 16 while homology of the ... between these HPV types In initial studies the vaccine has proven effective for prophylaxis against the initial infection by these types of HPV [20,21] Vaccine efficacy has been assessed by either ... is indicated by 18 The inability to obtain a product by nested PCR is indicated by a dash HPV type as determined by qPCR standard PCR performed using MY09/MY11 consensus HPV L1 primers Following...

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Generation of mouse graves ophthalmopathy model with full length TSH receptor plasmid and cytokine evaluation by real time PCR

Ngày tải lên : 07/10/2015, 09:55

... Immunoglobulin G IL2 Interleukin IL3 Interleukin IL4 Interleukin vi IL5 Interleukin IL1 0 Interleukin 10 KRH Krebs-Ringer HEPES MHC Major histocompatibility complex NK Natural killer PBS Phosphate ... denoted by triangles ( ) Letters indicate the amino acid in the wild-type receptor The asterisk (*) and double asterisk (**) indicate deletions resulting in a gain of function in hyperfunctioning ... pituitary gland secretes thyrotropin (TSH) which stimulates iodine trapping and increasing cAMP, thus thyroid hormone synthesis, and release of T3 and T4 by the thyroid gland TSH is regulated by feedback...

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Quantification of Microcystin-degrading Bacteria in a Biofilm from a Practical Biological Treatment Facility by Real-time PCR

Ngày tải lên : 05/09/2013, 10:15

... is included in the genus Sphingomonas (accession number AB11 063 5) The strain C-1 was isolated from Lake Hongfeng in China and this strain is included in the genus Sphingopyxis (AB 161 684) The ... bacteria The number of cells increased in fall, and the number of microcystin-degrading bacteria increased with the increase in the concentration of phytoplankton By using methods described in this ... an increase in the cell number of microcystin-degrading bacteria was observed in the biofilm in autumn at the practical biological treatment facility, but there is little information about the...

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Báo cáo khoa học: "Detection of anatid herpesvirus 1 gC gene by TaqMan™ fluorescent quantitative real-time PCR with specific primers and probe" pps

Ngày tải lên : 12/08/2014, 04:21

... ducks maintained 107 copies/g level at weeks postinoculation The copies of the liver, spleen and thymus were more than other tissues in gC DNA vaccine-inoculated ducks, while the copies of the duodenum ... (1-8), as indicated in the x-axis, whereas the corresponding Ct values are presented on the y-axis The correlation coefficient and the slope value of the regression curve were calculated and indicated ... lymph nodes and other organs in a short time by intramuscular injection of DNA vaccine [51,52] In this study, AHV-1 gC can be detected in all analyzed tissues at hour postinoculation, and the concentration...

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Tài liệu Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR pptx

Ngày tải lên : 15/02/2014, 04:20

... extracted by IPA The connection including CDH1, PLAU, and SMAD4 suggested to be related to cell adhesion by IPA The connection including TGM2, IL1 B, and PLAU and the connection including RAB25, ... component for the cell lines (PC1, PC2, and PC3), two of the three AD cells lines were close, whereas the third cell line was close in PC2 to the other two cell lines, but separate in PC1 and PC3 Specifically, ... robust increase in S100P expression, while the three SQ cell lines showed less or no increase Taking our data and the Kim et al data together, the remarkable and specific upregulation of S100P in...

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Tài liệu Báo cáo khoa học: Induction of uPA gene expression by the blockage of E-cadherin via Src- and Shc-dependent Erk signaling docx

Ngày tải lên : 19/02/2014, 05:20

... 227–240 ª 20 06 The Authors Journal compilation ª 20 06 FEBS 235 Loss of E-cadherin function induces uPA S Kleiner et al a3b1-integrins and E-cadherin besides other proteins In an indirect way ... are causally involved in tumor progression, the understanding of these underlying intracellular events is of importance In the present study, we explored the signaling pathway linking disruption ... Opin Genet Dev 10, 66 8 67 4 Pellegrini M, Pacini S & Baldari CT (2005) p66SHC: the apoptotic side of Shc proteins Apoptosis 10, 13–18 Pece S & Gutkind JS (2000) Signaling from E-cadherins to the...

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báo cáo hóa học:" Whole blood assessment of antigen specific cellular immune response by real time quantitative PCR: a versatile monitoring and discovery tool" potx

Ngày tải lên : 18/06/2014, 15:20

... respectively) induced by HCMV pp65495–503, EBV BMLF-1259– 267 , EBV LMP-24 26 434 and IM58 66 HLA-A0201 restricted peptides and the numbers of CD8+ T cells stained by specific multimers in the same ... reverse transcribed and amplified by qRT-PCR in the presence of primers and probes specific for the indicated genes and β-actin house keeping gene (panels A-F) Cytokine and chemokine gene expression ... genes encoding cytokines and chemokines and anti HBsAg serum titers in vaccinated healthy donors Correlation Correlation between expression of genes encoding cytokines and chemokines and anti HBsAg...

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Báo cáo sinh học: " Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc

Ngày tải lên : 18/06/2014, 22:20

... BestKeeper indices; even actin was included into our gene panel These findings demonstrate the usefulness of analysing a wide variety of reference gene candidates The inconsistent data regarding to the ... Corresponding to the recent estimation the SD of the CT value was highest for Act in of viruses, indicating that Act is no reliable reference gene in this setting In contrast, TBP and PPI displayed the ... participated in design and coordination of the study and helped to draft the manuscript All authors read and approved the final manuscript Material and Methods Virus culture and virus detection by real-time...

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báo cáo hóa học:" Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc

Ngày tải lên : 20/06/2014, 04:20

... BestKeeper indices; even actin was included into our gene panel These findings demonstrate the usefulness of analysing a wide variety of reference gene candidates The inconsistent data regarding to the ... Corresponding to the recent estimation the SD of the CT value was highest for Act in of viruses, indicating that Act is no reliable reference gene in this setting In contrast, TBP and PPI displayed the ... participated in design and coordination of the study and helped to draft the manuscript All authors read and approved the final manuscript Material and Methods Virus culture and virus detection by real-time...

5
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Báo cáo khoa học: "Effects of Benzo[a]pyrene, 2-Bromopropane, Phenol and 2,3,7,8-Tetrachlorodibenzo-p-Dioxin on Proinflammatory Cytokines Gene Expression by Mice Spleen Cells" doc

Ngày tải lên : 07/08/2014, 15:20

... enhancement of induction to no change [1, 7, 16- 18] In terms of the effects on IL- 6 and TNFα production, exposure to TCDD resulted in the similar patterns as observed for IL- 1 production [3, 6, 16, 18, ... at 94℃, 30 s at 60 ℃ and 90 s at 72℃ for β-actin, IL -1β and TNF α, 25cycles of 30 s at 94℃, 30 s at 56 and 30 s at 72℃ for IL- 6 and IFNγ The sequences of primers used are shown in Table PCR products ... anti-CD3 plus 10-6M B[a]P, 10-6M 2-BP, 10 -66 M phenol, or 10-9M TCDD for 18 h The amounts of IL -1β (A), IL- 6 (B), IFNγ (C) and TNFα (D) gene expression were determined by RT-PCR (upper part) and densitometry...

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Báo cáo y học: "Tumor necrosis factor alpha and epidermal growth factor act additively to inhibit matrix gene expression by chondrocyte" pptx

Ngày tải lên : 09/08/2014, 06:22

... potentiated by the presence of TNFα The distribution and arrangement of actin filaments were analyzed by phalloidin labeling An increase in stress fibers was observed in elongated cells; however, the ... implicated in mediating the effects of TNF-α and IL -1β on the expression of type II collagen [12,37] Disruption of the actin cytoskeleton with cytochalasin D or with latrunculin B results in an increase ... previously in cartilage or in other tissues The expression of matrix genes critical for maintaining structural and functional integrity of cartilage was downregulated additively by TNF-α and EGF...

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Báo cáo khoa học: "Real time PCR analyses of expression of E-cadherin, alpha-, beta- and gamma-catenin in human breast cancer for predicting clinical outcome" pps

Ngày tải lên : 09/08/2014, 07:21

... β-catenin and γ-catenin share approximately 65 % sequence homology and bind directly to the cytoplasmic tail of E-cadherin in a mutually exclusive manner α-catenin then links the bound β- or γ-catenin ... then added to the sections, which http://www.wjso.com/content /6/ 1/ 56 were incubated in the dark for minutes Sections were then counterstained in Mayer's haematoxylin and dehydrated in ascending ... positivity and negativity We determined the expression of E-cadherin, α-, β- and γcatenin in human breast carcinomas using real time quantitative polymerase chain reaction and investigated whether the...

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Báo cáo y học: " Profiles of cytokine and chemokine gene expression in human pulmonary epithelial cells induced by human and avian influenza viruses" doc

Ngày tải lên : 12/08/2014, 02:20

... The expression profile for IL- 8 and CXCL-10/IP-10 was similar to IL- 6 In H5N1/ 2004 infection, induction of cytokine/chemokine was prominent at the late phase (24 hours post-infection) Lam et ... 16- 1 16 16- 1 16 16- 1 16 H1N1/2002 4-450 4-450 4-450 4-450 4-450 4-450 and the effects were more prominent at the late phase of infection, particularly at 24 hours post-infection Also, the cytokine/chemokine ... biosynthesis may be interrupted by the viral components Cytokines and chemokines generally function in an autocrine (on the producing cell itself) or paracrine (on nearby cells) manner Cytokines...

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Báo cáo y học: " Detection of porcine parvovirus using a taqman-based real-time pcr with primers and probe designed for the NS1 gene" doc

Ngày tải lên : 12/08/2014, 02:20

... Reproducibility of the real-time PCR When the serially diluted standard plasmid (6. 00 × 108, 6. 00 × 1 06, 6. 00 × 104, and 6. 00 × 102 copies/μL) was subjected to the real-time PCR, the within-block ... authors read and approved the final manuscript Author details Laboratory of Marine Genetics and Breeding (MGB), Ocean University of China, Qingdao 266 003, China 2China Animal Health and Epidemiology ... designed for the NS1 gene Virology Journal 2010 7:353 Acknowledgements The study was supported in part by funding from the National High-tech R&D Program ( 863 Program-2007AA10 060 6) and the Chinese National...

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báo cáo khoa học: " Selection of reference genes for quantitative real-time PCR expression studies in the apomictic and sexual grass Brachiaria brizantha" ppt

Ngày tải lên : 12/08/2014, 03:20

... and sexual reproduction within Brachiaria makes it an interesting system for understanding the molecular pathways involved in both modes of reproduction The identification of genes involved in ... BbrizUBCE and BbrizTUB were again the more stable and the least stable genes respectively, while there was a slight difference in the order of stability of the other genes The second and third ... genes [ 26] The median Ct data in the 22 samples are shown in Figure 1, and the Ct variations among the samples for the different primers are shown in Figure The range and distribution of the Ct...

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