... Secondly, differences in the parasite species being subject to testing will also in uence the choice of concentration used in the screening process simply because of between species differences ... critical step in the process providing the capacity to systematically capture the scattered information available and keep track of the originating source Assemble a list of plant species occurring ... emergence and wide-spread incidence of chemical residues in human food and antimicrobial and anthelmintic resistance causing a surge of interest in the use of “natural” alternatives to chemicals in...
... metres in diameter contain mats of sulphur bacteria surrounded by two 15 LISA A LEVIN Figure Seep ‘ring’ consisting of bacterial mats in the core (~45 cm) and a concentric ring of vesicomyid clams ... aggregation Increasing patch age leads to a decline in primary producers (symbiont-bearing taxa) and increasing importance of secondary and higher predators, as well as non-endemic species Species richness ... biogeochemical turnover in gas hydrate-bearing sediments at Hydrate Ridge, Cascadia Margin: numerical modeling and mass balances Geochimica et Cosmochimica Acta 67, 3403–3421 40 ECOLOGY OF COLD...
... laser scanning microscope CMC carboxymethyl cellulose C- PAM cationic poly(acrylamide) cryo-SEM cryogenic scanning electron microscope CS cationic starch D.S degree of substitution DMA dynamic mechanical ... In contrast to xyloglucan and CMC, addition PDADMAC induced a sharp increase in frequency and a decrease in dissipation, indicating a reduction of the detected mass and an increase in elasticity ... the NFC by optical microscopy Scanning electron microscopy (SEM) The instrument used in the scanning electron microscopic examination of the composite film structures (Paper IV) was a Hitachi S4700...
... protein structural changes occur concomitantly with electron transfer [26] These changes are reflected by bands that have also been detected in redox-induced IR difference spectra of cytochrome cin ... corresponding to high local electric fields Furthermore, the local electric field strength in the electrochemical systems can readily be controlled by changing various parameters An increase in the electric ... data points in the low-field regime, both in electrochemical and in spectroelectrochemical measurements, one may conclude that the kinetic data follow the expected exponential distance dependence...
... (HA)-tag was placed at the N-terminus of the dUSP by cloning a double-stranded oligomer of the following sequence (upper strand, 5¢-AGCTACCCATACGACGTGCCAGACTACG CATCTCTG-3¢) into the BamHI site ... Fang et al Interaction of ultraspiracle and ecdysone receptor in hormone signaling a construct containing a single motif in the fi direction was used in this study Cell culture and transfections Spodoptera ... III-activated transcription by hydrophobic residue (L 314) in putative coactivator-binding hydrophobic groove of USP (A) Activity of DR1JHECore promoter in Sf9 cells cotransfected with the indicated...
... rheumatic diseases Competing interests The authors declare that they have no competing interests References Dieguez-Gonzalez R, Calaza M, Perez-Pampin E, Balsa A, Blanco FJ, Cañete JD, Caliz R, Carreño ... Genet 2007, 39 :147 7 -148 2 Lee EG, Boone DL, Chai S, Libby SL, Chien M, Lodolce JP, Ma A: κ Failure to regulate TNF-induced NF-κB and cell death responses in A20-deficient mice Science 2000, 289:2350-2354 ... Eyre S, Hinks A, Bowes J, Donn R, Symmons D, Hider S, Bruce IN; Wellcome Trust Case Control Consortium, Wilson AG, Marinou I, Morgan A, Emery P; YEAR Consortium, Carter A, Steer S, Hocking L, Reid...
... electrochemically oxidized to create active monomeric and dimeric species which react to form a conjugated polymer backbone The main characteristic of a conducting polymer is a conjugated backbone ... the chemical way of synthesis Electrochemical synthesis permits direct grafting of the conducting polymer onto the electrode surface, which can be of special interest for electrochemical applications ... as inactive packaging and insulating material However, this narrow perspective has been rapidly changing since a new class of polymer known as intrinsically conductive polymers or electroactive...
... (B) X-ray crystal structure of HIV-1 CA (Protein Data Bank code: 1E6J) Helices N1–N7 in the CA-NTD and C1 C4 in the CA-CTD are indicated [79] The CyPA-binding loop (red) and interdomain linker (green) ... p6 COOH 500 HIV-1 CA CyPA binding loop C- term N5 C3 N4 N7 C4 Interdomain linker N6 N3 C1 N term N-term reaction, to generate the correct conformation of proline, which is a rate-limiting step in ... within the cyclophi- C2 N2 N1 CA-NTD CA-CTD lin binding loop, have also been identified [41] A glycine-proline motif appears to be a prerequisite for binding the CyPA active site The speci c CA...
... Imidazoline binding in the MAO-A active site ˚ speed the calculations, the enzyme was cut to 12 A around the active site before each docking The docking procedure was tested using clorgyline as a control ... Keynes, UK) The linear secondary plots were fitted in Excel Spectra were recorded approximately after each addition in a Shimadzu UV-2101PC spectrophotometer in an anaerobic cuvette containing MAO-A ... Parini A & Lanier SM (1995) Imidazoline ⁄ guanidinium binding domains on monoamine oxidases ) relationship to subtypes of imidazoline-binding proteins and tissue-speci c interaction of imidazoline...
... 5¢-GATTGTCTACAACTGGG CACC-3¢ The amino acid at E180 in DCN E180Q is likewise substituted A cDNA construct for the truncated decorin species DCN Q153 (M1–Q153), in which six of a total of 10 leucine-rich ... chondroitin ABC lyase digestion) in HBS Decorin core protein concentrations were as indicated (B) Interaction with wild-type decorin in HBS buffer (solid lines) and in HBS buffer containing 15 lM ZnCl2 ... glycosaminoglycan-free core protein Evidently the glycosaminoglycan chain of decorin stabilizes the tertiary structure of the proteoglycans thereby causing difference in binding affinity As decorin...
... Gacto, M (1994) Cyclic AMP signalling pathway and trehalase activation in the fission yeast Schizosaccharomyces pombe Microbiology 140 , 146 7 147 2 24 Stoscheck, C. M (1990) Quantitation of protein ... (CCGCTCGAGCCTAACGG TGTGGAATAC, which hybridizes at positions 715–732 in the tps1+ ORF and shows an internal XhoI site), and the 3¢ oligonucleotide TAF-3 (CTACGGCGGCCGCCCGAGC TAGAATTCATCGA, which hybridizes ... J., Vicente-Soler, J & Gacto, M (1997) Protein kinase Sck1 is involved in trehalase activation by glucose and nitrogen source in the fission yeast Schizosaccharomyces pombe Microbiology 143 , 2457–2463...
... “arithmetic” aspect alluded to above In this text we shall be concerned with both aspects, but our original contribution concerns the “motivic” aspect, more precisely, in finding a way to circumvent ... Q-motive structure of M induces a direct sum decomposition H(M, C) = H(M, C) σ σ∈Hom(Q ,C) which respects both Q0 -structures The notation H(M, C) σ refers to the complex vector subspace of H(M, C) where ... Periods of Hecke Characters, Lecture Notes in Math 1301, SpringerVerlag, New York, 1988 [20] L C Washington, Introduction to Cyclotomic Fields, Second edition, Grad Texts in Math 83, Springer-Verlag,...
... in the presence (+) or absence (–) of EF-P protein The positions of chemical attack were determined using reverse transcriptase and a 22-nucleotide cDNA primer 5¢-TCTCCAGCGCCACGGCAGATAGG GACC-3¢, ... provides information regarding the quality of the search match, including the acquired MS ⁄ MS scan numbers, final candidate search cross-correlation (X-corr) score, the normalized delta cross-correlation ... of action of a factor from Escherichia coli required to reconstruct translation Proc Natl Acad Sci USA 82, 1648–1652 Ganoza MD, Cunningham C & Green RM (1995) A new factor from Escherichia coli...
... presence of the catalytic tetramer C1 r2 C1 s2 in the C1 complex that hinders the interaction of the C1 q subunit with the polysaccharide According to this hypothesis fucoidan should then interact ... Biochem 270) Altogether these results indicate that the collagen-like region of C1 q contains distinct sites for the binding of DNA and for the binding of the catalytic tetramer C1 r2 C1 s2 Interactions ... resulting C1 q-DNA complex was resistant to the stretching and combing of DNA on PMMA surfaces, indicating the strength of the interaction A continuous succession of fluorescent C1 q could be observed...
... aliquots containing equal amounts of protein was determined by precipitation with trichloroacetic acid Extracts containing equal amounts of protein were diluted in Buffer C [50 mM Tris/HCl, pH ... paraformaldehyde in NaCl/Pi, pH 7.4 for 15 After a single rinse with NaCl/Pi, cells were permeabilized in NaCl/Pi containing 0.1% (v/v) Triton X-100 for min, rinsed once in NaCl/Pi then incubated with ... appearance, being generally concentrated in the perinuclear region Levels of both hsp25 (Fig 4C) and aB-crystallin (Fig 4D) were increased with MG132, each showing a distinct, reproducible localization;...
... contradictory reports on which domains of CD2AP are involved in the nephrin-binding [27,28] Consequently, it is possible that the biologically more critical interaction occurs between podocin and CD2AP, ... IQGAP1 is a scaffolding protein connecting Ca2+ ⁄ calmodulin and Rac1 ⁄ Cdc42-mediated signalling and cytoskeleton [51] Very interesting is the recent observation that IQGAP1 interacts with CLIP-170, ... the nephrin intracellular domain responsible for IQGAP1 binding All constructs containing the C- terminal half of the intracellular domain (amino acids 1167–1256) bound IQGAP1, whereas we could not...
... pcDNA3.1/Zeo(+) (Invitrogen) A schematic diagram of the constructed chimeric receptors is shown in Fig 1A The constructs were carefully designed to maintain the disulfide bond connections within ... antibiotics The mammalian expression vectors, which were constructed to express the wild-type or chimeric receptors, were introduced into CHO cells by the LipofectAMINE method (Life Technologies Inc) ... the chimeric receptors Monolayers of CHO cell clones expressing the wild-type ErbB-1, ErbB-4, or the indicated chimeric receptor were incubated with 125I-labeled EGF at C for h After the incubation,...