... disputable relevance of our results to pathogenesis and clinical manifestations of atherosclerosis. The finding of C. pneumoniae in serum of ACS patients does not establish causality for the pathogen ... from the same volume of whole serum with QIAmp Blood Midi Kit (QIAGEN, Valencia, CA) according to the manual. Bacterial DNA was also extracted fromthe bac-terial particles trapped fromthe ... may originate from destructed mononuclear cells and/or macrophages residing in the atherosclerotic plaques. However regardless ofthe origin, we can claim that the presence of virulent chlamydial...
... final part of the outbreak data, as there is often a period lasting ten or more weeks when there arefew cases.We investigated whether the removal ofthe lowest-valued points fromthe datasets ... differences between the behavior ofthe SIR model and the data. Potentialsources of these differences include inadequacies ofthe mathematical model to de-scribe the process underlying the data and ... proportional to the square ofthe predicted incidence: wj= 1/[z(tj; θ)]2.On the other hand, if ρ = 1/2, then the weights are proportional to the rec iprocal of the predicted incidence; these correspond...
... is the cross-sectional average ofthe bank-specific variable αi of those banks forwhich there is an observation in month t. As the composition of this sample changes in the course of time, the ... (column 3) and the model with only the regulation variables (column 4). The R2 of the full model is17.24%, i.e. the combined contribution ofthe systematic factor and the regulation to the total ... constantand the own funds decrease or increase.With the help ofthe two other specifications, it is possible to disentangle the contri-butions ofthe systematic factor and ofthe regulation....
... applying the optical principle of the persistence ofthe image on the retina, as also the continuous progress of the syntheses of movement as compared with the state of photographic techniques. The fact ... to have the last word in the ar-gument with death by means ofthe form that endures. If the history ofthe plasticarts is less a matter of their aesthetic than of their psychology then it will ... of all is the obstinateresistance of matter to ideas rather than of any help offered by techniques to the imagination ofthe researchers. Furthermore, the cinema owes virtually nothing to the...
... catalytic residues The active site cavity in IPDC extends fromthe thiazoliumring ofthe cofactor to the surface ofthe protein. The entrance ofthe active site cleft is covered by the C-terminalhelix ... on the other hand involving the C-terminal part ofthe polypeptide chain (Fig. 7). In the latter, differences in the conformation ofthe loop betweenFig. 6. Stereo picture ofthe model of the a-carbanion/enamine ... However, the packing of the two dimers in the tetramer is different from that of the PDCs of known structure, best described as a 20° rotation of one dimer towards the other when compared to the Z....
... NeuAc.Discussion The presence of naturally occurring agglutinins in the hemolymph of several crustaceans has been well knownsince the beginning ofthe 20th century [34]. An evaluation of the literature ... °C. The eluant wascollected at a rate of 0.6 mLÆmin)1.Thecolumnwaswashed with HSB until the A280 of the effluent was < 0.002. The column was further washed with LSB at 4 °C until the A280 of ... containing 100 lLof100mMCaCl2at a rate of 0.3 mLÆmin)1. The presence of calcium chloride wasrequired in the collected fractions because the lectin wasunstable in the presence of EDTA. The fractions...
... proteinsimplicated in the biosynthesis ofthe aminonucleoside moiety of A201A. Here we report the sequencing of a total of 6946 bp, which include the ata genes most likely implicatedin the biosynthesis of this ... it. Further sequencing ofthe atacluster could provide an insight into the biosyntheticpathway ofthe other moieties of A201A and provideprobes which may be useful to identify genes of the hygromycin ... in the biosynthesis of the D-rhamnose and a-p-coumaric acid moieties of A201A. Further sequencing of 2 kb of DNA downstream of ard1 has disclosed a region which might contain one end of the...
... 269) 427[26,27]. Then, the biosynthesis ofthe core region of LPS from A. baumannii strain ATCC 19606 should proceed with the introduction of a third Kdo residue to O-5 ofthe secondKdo. This ... mechanism of the transfer ofthe third Kdo residue has not yet beenidenti®ed.In addition to the work on the determination of the chemical and antigenic structures of O-speci®c polysaccha-rides from ... to a completestructure ofthe carbohydrate backbone ofthe LPS from A. baumannii strain ATCC 19606 as depicted in Fig. 4. The structures o f four core regions from LPS of d ifferentAcinetobacter...
... locks the enzyme in the active form for CTP synthesis andthereby stimulates the kcat of the reaction. Since GTP actually willactivate the uncoupled glutaminase reaction the structure of the monomer ... wassimilar to that ofthe overall CTP synthesis reaction. Itwas concluded that the effect of GTP was mainly toenhance the rate of chemical steps ofthe glutaminasereaction. The finding that the kcatvalue ... as the displacement ofthe baseline. The peaksobserved at each injection time are derived fromthe heat of dilution of glutamine into the reaction cell. (B) The heat generated by hydrolysisof...
... over the minds ofthe children by means of these dolls. She fell at once into the idea ofthe children in regard to them, and treated them always as if theywere real persons; often speaking of them ... perseveringly followed up by the watchful efficiency ofthe mother. We can not substitute the action ofthe child for that ofthe parent in the work of early training, but we can often derive very greatadvantage ... a wood. Either of them led to the river. But there was a brook to cross, andfor one of these paths there was a bridge. There was none for the other. George said that they would take the former....
... Pi. The effect of these samples on cyto-kine production by PBMCs activated by LPS was thenassessed as described above.Prediction of interaction sites The prediction of binding sites fromthe ... amphipathicity) ofthe helices. The mean surface accessibility ofthe predicted epitopes on the Iris 3D structures was calculated by averaging the accessiblesurface area ofthe peptide residues using the Shrake ... meal from a variety of verte-brates, ranging from amphibians to mammals, andincluding domestic animals and humans [2]. It is the one ofthe vectors of Borrelia burgdorferi, the agent of Lyme...
... molecular mass ofthe native peptide wasconsistent with the oxidation ofthe corresponding thiolgroups, in order t o form the last missing disulfi de bridge.Furthermore, this is one ofthe c onstant ... shown thereafter. The first group(11 sequences) is fromthe a-ScTXs, t he second is fromthe b-ScTXs. Birtoxin is the sho rtest. The de pressant and the long-chain ex citatory are in the last two ... ofthe N-terminal segment with C5 ofthe b2 stran d [ 9]. The fourth disulfide bond is established between C1 and C8, of the N- with t he C-terminal region. The excitatory insecttoxins lack the...
... lower thanthat ofthe L -rich apoferritin of horse spleen (t1/2¼ 60 0 s)[19]. The iron-oxidation capacity is maintained byT. bernacchii apoferritin at low temperature; the rate of the reaction ... Interest-ingly, the 262 nm phenylalanine band is of opposite sign t o the protein a t pH 7.0 (Fig. 4B). The f ar-UV CD spectrum of T. bernacchii apofer ritin at pH 2.5 shows a modest blueshift ofthe zero ... fishferritins on the basis ofthe available sequences.A further distinctive property of t he fish H chains knownto date relative to those o f mammals appears to be the lack of the four-amino-acid...
... electronic condition of the Fe-S cluster through the protein scaffold, resultingin an alteration ofthe signal rhombicity ofthe EPRspectrum. The divergence ofthe g-value from that of the whole cell ... upstream of the GAF domain are the potential ligands of the Fe-S cluster. Consequently, the binding ofthe ATPanalog and the subsequent rearrangement ofthe inter-domain interaction affects the ... level ofthe lacZ gene immediately after the addition of o-phen to minimize the effect ofthe growthinhibition by o-phen on the transcriptional activity of VnfA. Figure 5 shows the time course of...
... APTTreagent in the absence of Ca2+. In these experiments, the kallikrein–kinin system had been activated before the addition of triafestin-1 or triafestin-2. In the absence of Ca2+, factor ... chro-mogenic substrates. Neither triafestin-1 nor triafestin-2inhibited the amidolytic activities of any of theseplasma proteins (data not shown). Therefore, we exam-ined the effects of triafestin-1 and ... HK. The fact thatthese three unrelated blood-sucking arthropods sharesuch similar bioactive substances as major components of the saliva emphasizes the biological importance of inhibition of the...