... BỘ CÔNG THƯƠNG TRƯỜNG ĐẠIHỌCCÔNG NGHIỆP THỰC PHẨMTPHCM KHOA CÔNGNGHỆSINHHỌC & KỸ THUẬT MÔI TRƯỜNG KHÓA LUẬN TỐT NGHIỆP CHUYÊN NGÀNH CÔNGNGHỆSINHHỌC KHẢO SÁT SỰ ẢNH ... thước tế bào động vật thường lớn, trung bình khoảng 10 μm, lại vách nên tế bào động vật có tính chất học yếu Do đó, nuôi cấy cần nhẹ nhàng, tránh phá vỡ tế bào (Phan Kim Ngọc, 2002) ... Namalwa) 25 Sự sinh trưởng của tế bào động vật nuôi cấy Sự sinh trưởng của tế bào động vật in vitro thường trải qua pha: - Pha chậm (Lag phase) giai đoạn tế bào được đưa vào môi trường...
... were cultured in Dulbecco’s MEM, supplemented with 10% fetal bovine serum (GibcoBRL), 100 lM 6-thioguanine (Sigma), 100 lgÆmL)1 streptomycin, and 100 lgÆmL)1 penicillin at 37 °C in a humidified atmosphere ... (Biochrom KG), 100 lgÆmL)1 streptomycin, and 100 lgÆmL)1 penicillin at 37 °C in a humidified atmosphere with 5% CO2 on plastic tissue culture grade flasks (Nunc) N18TG2 cells (DSMZ ACC 103 ) were cultured ... : 100 0) For detection of the VRL-1-GFP fusion protein anti-GFP polyclonal goat IgG (Abcam, : 5000) was used as first and mouse anti-(goat-IgG) Ig coupled to horseradish-peroxidase (Pierce, : 100 0)...
... and Translational Accuracy 102 D ISCUSSION 103 3.1 Validity of the Model 103 3.2 Translational Efficiency and Accuracy on Codon Usage Bias 104 3.3 How Optimized Are the ... Optimized Are the Translational Machinery? 105 11 EVOLUTION OF A M I N O ACID USAGE 107 I NTRODUCTION 107 AMINO ACID USAGE BIAS 109 12 PATTERN OF NUCLEOTIDE SUBSTITUTIONS ... software listings: http://evolution.genetics.washington.edu/phylip/software.html http://biosci.biosc.lsu.edu/general/software.html http://darwin.eeb.uconn.edu/molecular-evolution.html http://www.york.biosis.org/zrdocs/zoolinfo/software.htm...
... gland, activation of iPLA2 by P2X7 agonists ATP or the ATP analog Bz-ATP [2',3'-O-(4-benzoylbenzoyl)adenosine 5'-triphosphate] was observed (145) ATP or Bz-ATP induces the release of [3H]AA to the ... fimetion ATP could affect iPLA2fl function by at least three mechanisms First, ATP could bind to the ATP-binding domain of iPLA2fi to stimulate its intrinsie enzymatic aetivity Second, ATP could ... Turk, Biochemistry 32, 327 336 (1993) 106 Z Ma, S fiamanadham, J Corbett, A Bohrer, R W Cross, M L Mcdaniel, and J Turk, J Biol Chem 271, 102 9 -104 2 (1996) 107 Z Ma, S Ramauadham, Z Hu, and J Turk,...
... However, if n = 1,3,7 is odd and m = 2n − 1, or if, for example, (m,n) = (8,4),(9,4),(9,3), (10, 4),(16,8),(17,8), (10 + n,n) for ≤ n ≤ 11, or (24,6), then there exists a map f : Sm → Sn such that = N( ... Fundamenta Mathematicae 184 (2004), 187–203 [10] , Selfcoincidences in higher codimensions, Journal f¨ r die Reine und Angewandte Matheu [11] matik 576 (2004), 1 10 , Nielsen coincidence theory in arbitrary ... Mathematik, 2003, http:/www.math.uni-siegen.de/topology/ ’ur publications.html , Nonstabilized Nielsen coincidence invariants and Hopf-Ganea homomorphisms, preprint, [13] 2005, http://www.math.uni-siegen.de/topology/publications.html...
... data of the patient on admission Patient Normal range Hb 11.4 12-14 g/dl WBC 8.1 *10/ ml 4 -10. 8 × 103 /ml Ca 13.3 8.5 -10. 3 mg/dl P Creatinin 3.5 1.9 2.5-4.5 mg/dl 0.5-1.2 mg/dl PTH 15 15-65 pg/ml LDH ... male 18.8 13 (
... abbreviation server http://bionlp.stanford.edu/abbreviation/ [35] AbGene Protein name tagger ftp://ftp.ncbi.nlm.nih.gov/pub/tanabe [29] ABNER Protein/Gene/DNA/RNA/cell tagger http://www.cs.wisc.edu/~bsettles/abner/ ... literature database and search http://www.scopus.com/scopus/home.url [93] Textpresso C elegans literature information retrieval and extraction tool http://www.textpresso.org/ [48] XplorMed Explores ... service http://biomail.sourceforge.net/biomail/ [12] BioRAT Biology information extraction tool http://bioinf.cs.ucl.ac.uk/biorat/ [81] BITOLA Literature-based biomedical discovery system http://www.mf.uni-lj.si/bitola/...
... 2.98; sd = 1.321) Strongly Agree Agree Neutral Disagree Strongly Disagree 29 39 20 1010 23 32 25 10 12 29 30 21 10 20 19 28 23 11 22 32 30 19 21 29 27 22 16 32 24 18 37 34 16 19 32 30 12 33 20 ... restaurants; and suspending religious services (Glass, Glass, Beyeler, & Min, 2006; World Health Organization Writing Group, 2006) The goal of these methods is to prevent the transmission of disease ... them reported any deaths from the deadly pandemic influenza of 1918-1919 (Merk et al., 2006) Merk et al (2006) determined through their analysis, that protective sequestration, if enacted early...
... siRNAs………………………………………………………… 100 -102 Table 4.5 HBV-induced gene changes…………………………………… 106 Table 4.6 Summary of studies of siRNA’s effect on HBV…… 108 Table 5.2 Ratios of absorbance 260/280 for the 10 pairs ... Discussion…………………… …………………… …………………… …… 103 4.5.1 Inhibition of HBV Gene Expression by siRNAs……………………………… 103 4.5.2 Microarray Analysis of HBV-induced Changes in Gene Expression………… 105 4.5.3 Specificity of ... per age standardized 100 ,000 population) Other areas of high incidence rate are Central Africa (~41 per age standardized 100 ,000 population), Japan (~31 per age standardized 100 ,000 population),...
... analysis http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE 1107 3 last accessed July 10, 2014 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GPL6173 last accessed July 10, 2014 http://www.genome.jp/kegg/ ... 34 http://www.plantcyc.org/ last accessed April 7, 2014 http://mapman.gabipd.org/web/guest last accessed April 7, 2014 36 http://www.cls.zju.edu.cn/pair/ last accessed April 7, 2014 37 http://www.genemania.org/ ... accession id: GSE441712 http://www.gatc-biotech.com/en/index.html last accessed July 10, 2014 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE44171 last accessed July 10, 2014 31 3.1.1.2 Microarray...
... in the presence of, for example dGTP, then the ends of the vector will 126 PCR 3' 5' PCR-amplified product CCCCCC 5' 3' Terminal transferase CCCCCC +dCTP + dGTP 3' Vector 5' GGGGGG 5' GGGGGG Vector ... 6.1 Blunt-end polishing of PCR fragments MATERIALS T7 DNA polymerase 10 × T7 DNA polymerase buffer 100 mM MgCl2 mM each dNTP mix Distilled water EQUIPMENT Microcentrifuge tube Microcentrifuge ... microcentrifuge tube: ● up to 40 µl of purified PCR product; ● µl of 10 × T7 polymerase buffer; ● µl of 100 mM MgCl2; ● 2.5 µl of mM dNTP mix; ● unit of T7 DNA polymerase; ● water to 50 µl Collect reagents...
... Gly-Gly-L-Leu Gly-L-Leu-L-Tyr L-Leu-Gly-Gly 100 0 116 58 755 39 74 155 447 327 58 443 360 26 543 301 46 0 100 23 254 30 110 1235 16 83 67 105 166 823 101 385 76 0 0 a The data from this study The ... a forward primer (A), 150 pmol of a reverse primer (B or C), · G-Taq buffer, 10 nmol each of dATP, dGTP, dCTP and dTTP, and 0.5 U G-Taq DNA Polymerase (Cosmo Genetech Co., Seoul, Korea) For PCR ... primer, 15 pmol of a reverse primer, 1· PCR Gold buffer containing 2.5 mm MgCl2, 10 nmol each of dATP, dGTP, dCTP and dTTP, and U AmpliTaq Gold (Applied Biosystems Japan Ltd) For PCR the following...
... unicast 21.7 Example 21.1 A host with IP address 130.23.43.20 and physical address B2:34:55 :10: 22 :10 has a packet to send to another host with IP address 130.23.43.25 and physical address A4:6E:F4:59:83:AB ... 21.8 Figure 21.5 Example 21.1, an ARP request and reply 21.9 Figure 21.6 Proxy ARP 21 .10 Figure 21.7 BOOTP client and server on the same and different networks 21.11 Note DHCP provides static ... generated for a datagram having a special address such as 127.0.0.0 or 0.0.0.0 21.17 Figure 21 .10 Contents of data field for the error messages 21.18 Figure 21.11 Redirection concept 21.19 Figure...
... Current Science, 81(12): 1534-1546 UN Works- The Global Water Crisis 2 010 From< http www.un.org> (Retrieved October 29, 2011) 109 Gleick PH, Adams DB The Report of the Water Sector Assessment Team ... Water Treatment200 From (Retrieved March 20, 2009) Home Page of Mining and Water Pollution 2011 From (Retrieved ... 423-427 Official Home Page of Delft University of Technology 2006 New Water-Purification Method Promises Radical Improvement From (Retrieved March 9, 2009)...