... polymerization of styrene andthe blending of PS and SPS polymers have affected the surface structure ofthe PVDF membrane obviously The cross-section morphologies ofthe PVDF membrane, the PVDF-PS ... Thermal analysis ofthe PVDF-SPS composite membrane The TG analysis curve is presented in Figure for the investigation ofthe thermal stability ofthe PVDF membrane, PVDF-PS membrane, andthe PVDF-SPS ... to prepare the PVDF composite membrane The structure andthe morphology ofthe PVDF-SPS composite membrane were characterized The proton conductivity andthe methanol permeability ofthe PVDF-SPS...
... important in the generation ofthe correct class of antibodies by B-cells, and for the elimination of extracellular pathogens, such as helminths and other extracellular parasites [2] Th17 is the most ... domain and SH2 domain of stat6, andthe zinc-finger domain, leucine-zipper domain and fork-head domain of foxp3 In addition, for stat6 and foxp3, there were a few other conserved features Within the ... tissue of mouse and spleen and thymus of human and mouse [11,37] Foxp3 is also highly expressed in lymphoid organs, such as the thymus and spleen [49], and its expression within a variety of other...
... cm2 per residue using the mean residual weight of GlcNAc [24] Further characterizationof chitosan andthe depolymerization products Further characterizationof chitosan andthe depolymerization ... comparison of band absorbance Absence of a sharp and convoluted spectral band around 3600–3000 cm)1 in the spectra of both chitosan and LMWC indicated the absence of free -OH groups andthe involvement ... (moles/L) · volume of titrant (mL)/mass of sample (g) andthe level of deacetylation was calculated by comparison between the number of free amino groups (per unit weight ofthe sample) andthe equivalent...
... shown in the inset, the progressive decrease ofthe molecular ellipticity at k ¼ 200 nm (the absorption region ofthe peptide bond) reflects the occurrence of a transition between 35° and 55 °C ... one PHR and one PHO (abc) protomer The hypothesis of a direct PHI–phenol interaction is quite unlikely, because ofthe fact that the addition of phenol does not alter the emission spectrum of PHI ... identity between the N-terminal sequence of PHI and those of ferredoxin-like proteins belonging to other oxygenases On the contrary, the N-terminus of PHI is identical to that of MopN from A calcoaceticus...
... GroEL from 15 and 30 lg ofthe cell lysate without (lanes and 3) or in the presence of ATP (lanes and 4) was detected with anti-UP12 Ig Samples (3 and lg of total proteins) ofthe cell extract ... UP12 and UspA is not very pronounced, the following observations support the classification of UP12 as a member ofthe UspA family Sub-cloning, purification, andcharacterizationof UP12 and its ... anti-GroEL Ig [27] The high yield ofthe UP12-GroEL complex isolation andthe ATP-mediated dissociation ofthe complex strongly support the suggestion that the two stress proteins GroEL and UP12 interact...
... protein content in their latex; and (2) the presence of other cysteine proteinases or isoforms in the latex To investigate these two hypotheses, the protein concentration per milligram of dried latex, ... tapped for the first time, we are not aware ofthe frequency of tapping in other studies These and probably several other, as yet unknown, factors affecting the activity and concentration of latex ... sequences ofthe proteins VXH-I, VXH-III, VXH-IVa and VXH-IVb are shown in Fig 6, and are compared with the known N-terminal sequences ofthe cysteine proteinases of C papaya and V cundinamarcensis The...
... account the possible size ofthe mlp gene family andthe number of gene variants considered in the construction ofthe M1 and M2 primers, the error may be not large In our estimate, one copy ofthe ... role ofthe sequence (Fig 2) The sequences ofthe catalytic A-chain ofthe MLp toxins were found to differ However, the amino acids forming the catalytically active center ofthe A-chains ofthe ... (and BclI, data not shown) digests gave a very similar pattern of five bands, one of which was also more intensive than the others Thus, the different intensity ofthe bands may be the result of...
... 205 and 215 nm, indicating that Rv1399c is composed of 22% of a-helices, 25% of b-strand and 39% of random coil The catalytic triad is located at the bottom of a small ˚ ˚ pocket, 20 A length and ... experimentally Indeed, the presence of Arg213 along with the proximity ofthe charge relay Asp260 within the active site could be responsible for the modification ofthe catalytic properties ofthe catalytic ... by the amides of Ala163, Gly89 and Gly90, the latter’s being found in the HGGG consensus sequence motif characteristic of members ofthe HSL family [35] Our model shows that the architecture of...
... experiments The last exon is composed of a coding sequence of 193 bp andof an untranslated region of at least 131 bp The size ofthe 24 introns ranges from 79 bp to about 16 kb The size ofthe largest ... fragments of 1.6 and kb named 2350 and 2355, respectively They both overlap the 3¢ end ofthe repeat region To sequence the 5¢ region, we performed RT-PCR using NAU972 (designed from SMC) andthe antisense ... using random hexamers or oligo(dT) primer Several oligonucleotides (Fig 1) were designed by comparison ofthe similar regions in the 3¢ regions ofthe gene coding for the rat SMC andofthe human...
... other species, except in the case of sheep tryptases and [13], where the identity reaches 82–83% The major and more significant difference between BLT and BLCT resides at positions 188–189 ofthe ... Furthermore, analysis of BLCT and BLT expression in bovine heart and spleen has shown that both enzymes are present in these tissues at the mRNA level The simultaneous expression ofthe two isoforms ... agarose gel electrophoresis andthe bands eluted, purified and subcloned in the PCRIITM TOPO vector containing the lac promoter andthe b-galactosidase gene, using the TA Cloning Kit (Invitrogen,...
... Glycosylation was 0.5% ofthe molecular mass ofthe native laccase andand 10% for the recombinant laccase In the heterologous production ofthe P cinnabarinus laccase in P pastoris [14] or the T villosa ... performed to determine the isoelectric point The protein was, as the wild-type, very acidic andthe pI estimated to be 3.7 Effect of temperature and pH on laccase activity Studies ofthe recombinant ... Effect ofthe temperature on the activity ofthe purified laccase Various temperatures in the range of 25 °C to 85 °C were tested with 500 lM ABTS as the substrate 20 kDa Fig SDS/PAGE gel analysis of...
... downstream from His80 in the vicinity ofthe zinc center and is assumed to clamp the imidazolium ring of His80 Glu149 is in the immediate vicinity ofthe Glu150 ofthe zinc center and is assumed to ... examined the V alginolyticus PepD protein In the present study, we present the cloning and expression ofthe V alginolyticus pepD gene, the purification and biochemical characterizationofthe produced ... through the activation ofthe water molecule by both the zinc ions and transfer ofthe proton to the Glu149; (d) the carbonyl oxygen will be bound in an ‘oxyanionbinding hole’ formed by Zn1 and the...
... forward primers Designand synthesis ofthe AtSMT sequence adapted to the codon usage of S cerevisiae was performed by GENEART Construction ofthe multicopy-plasmid pDIONYSOS The leu2-d marker ... infected with mL ofthe virus stock After approximately one-third ofthe cells were lyzed (72 h of incubation), they were harvested and pelleted The cells were resuspended in 1.5 mL of buffer (100 ... codon usage ofthe Arabidopsis gene as well as the nature ofthe signal peptide andthe sequence motif around the translation start as critical parameters for efficient expression of AtSMT in...
... dsRNA They suggest that the complete silencing ofthe gene transcript could not be achieved due to dilution of dsRNA in the feeding stage ofthe female tick [31] On the other hand, Acosta and coworkers ... observed the amplification by RT-PCR of a defined band of 260 bp in the saline treated group only These results suggest the possible complete degradation ofthe CHH transcript because of dsRNA ... Pharmacia) according to the instructions ofthe manufacturer Northern blot analysis Northern blot analysis was used to characterize the expression ofthe shrimp CHH gene Ten micrograms of each RNA sample...
... from the experiments shown in Figs and In each experiment the tissue with the highest expression level was set to 100 The height ofthe other bars indicates the relative amount of NICN1 mRNA in the ... dependent on the metabolic state ofthe organ As the RNA for the Northern blot andthe RT-PCR analysis had been derived from two different anonymous human donors the differences in the observed ... enzyme of glycine metabolism [4,11] The promoters ofthe canine and murine NICN1 genes lack a TATA box-motif In both species, the sequences in the promoter regions fulfill the criteria of CpG islands...
... Gly-Ser, in-frame with the codon for Pro2 ofthe receptor cDNA The codon for the last used amino acid ofthe receptor (Ser412 in case ofthe full-length receptor and Ala316 for the truncated receptor) ... concentrations of 10±20 times their Ki value Without ligand, a half-life of 33 days was obtained This increased to 77 days in the presence of NECA (agonist) and 53 days in the presence of theophylline ... temperature and using high concentrations ofthe weak antagonist theophylline combined with low ¯ow rates The ®nal ionexchange step allowed removal of theophylline, and a reduction of volume and detergent...
... bacteriophage T5+ were taken from the collection ofthe Laboratory of Molecular Microbiology ofthe Institute of Biochemistry and Physiology of Microorganisms (IBPM RAS) Strains of E coli K12, B subtilis, ... aspartic acid ofthe enzyme and an acidic N-terminal amino acid ofthe substrate [26] The affinity of bacteriophage T5 endolysin for d-glutamic acid of peptidoglycan andthe ability of endolysin ... Asp73, Asp130, and His133) that are typical for metallopeptidases ofthe M15 family and take part in the binding ofthe metal ion in the process of catalysis [28] This is the first example of an l-alanoyl-d-glutamate...
... of degenerate and specific primers and by rescreening the cDNA libraries The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb ofthe respective 3¢ ends and included 3¢-UTRs and poly(A)-tails ... representation ofthe full length XHIVEP1 andthe Myc-tagged (MT) deletion mutants Black and white boxes indicate the position ofthe Znf domains andthe serine-rich stripe, respectively The relative ... bold The position ofthe terminal amino acid for the depicted sequence is indicated to the right (Fig 6B, compare lanes and with and 6) Mutation ofthe GAGA motif only slightly altered the ZAS-N...
... start ofthe oraS gene and a BamHI site into the end ofthe oraE gene Genomic DNA was purified from C sticklandii by phenol/chloroform extraction methods [4] The coding regions for the S and E ... determined by measuring the apparent Km value of D-ornithine at 25, 50, 100, 200 and 400 lM ofthe inhibitor For the measurement ofthe activity ofthe substrate analogues, an HPLC and NMR-based method ... copies ofthe argU, ileY, and leuW tRNA genes The coprecipitation of OraS and OraE might imply that (a) the apoenzyme or OraE is not properly folded; and (b) the noncovalent interaction between these...
... [8–10]) Thermal unfolding ofthe protein was monitored at 203 nm at a heating rate of 60 °CÆh)1 Effect ofthe recombinant protein on the activity of proteases The activity ofthe proteases on synthetic ... (JASCO) Fig Alignment ofthe sequences ofthe second Kunitz-modules ofthe human and fugu WFIKKN proteins (wfikkn_hu_2; wfikkn_fugu_2) with the second Kunitz-domains ofthe human andthe two fugu WFIKKNRP ... determinant ofthe hydrophobic S¢2 binding pocket of trypsins is the side-chain of Tyr151 [18] The importance of this residue is underlined by the fact that in the case ofthe second Kunitz-domain of...