Molecular biology, a project approach s karcher (AP, 1995)

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... Oval Road, London NW 7DX Library of Congress Cmaloging-in-Publication Data Karcher, Susan J Molecular biology : a project approach / by Susan J Karcher p cm Includes index ISBN 0-1 2-3 9772 0-7 .. .MOLECULAR BIOLOGY A Project Approach This Page Intentionally Left Blank MOLECULAR BIOLOGY A Project Approach SUSAN J KARCHER Department of Biological Sciences Purdue University West Lafayette,... perform a new exercise each laboratory period It is that projectoriented approach that I bring to this manual Susan J Karcher West Lafayette, Indiana e XVll This Page Intentionally Left Blank ACKNOWLEDGMENTS
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Xem thêm: Molecular biology, a project approach s karcher (AP, 1995) , Molecular biology, a project approach s karcher (AP, 1995) , CHAPTER 1. TRANSPOSON MUTAGENESIS OF Escherichia coli, Protocol 1.2: Making a Phage Stock—Growing λ-Tn5', Protocol 1.4: Isolation of Auxotrophs–Replica Plating, Toothpicking, or Screening on 2 EM Plates, Protocol 1.6: Analysis of Auxotrophs Using a Literature Search, Protocol 2.1 b (Optional): Restriction Enzyme Digestion of DNA to Be Cloned, Protocol 2.3: Large-Scale Plasmid Isolation Using Alkaline Lysis, Protocol 2.6b: Alkaline Mini-Prep Procedure for Isolating Plasmid DNA, Protocol 3.1 b: Bidirectional Blotting: A Sandwich Blot, Protocol 3.2: Isolation of DNA Fragments by Electroelution, Protocol 3.4a: Hybridization and Detection of Labeled Probe—A Biotin-Labeled Nonradioactive Probe and Chromogenic Substrate, Protocol 3.4b: Hybridization and Detection of Labeled Probe—A Biotin-Labeled Nonradioactive Probe and Chemiluminogenic Substrate, Protocol 3.5: Standard Southern Blot Hybridization with 32p-Labeled Probe, Protocol 4.1: Plant DNA Extraction Mini-Prep Procedure, Protocol 4.2: Steps of a Genomic Southern Blot, Protocol 5.1: RNA Extraction from Plant Leaves, Protocol 5.3: RNA Gel: A Denaturing Formaldehyde Gel, Protocol 5.5: Standard Northern Blot Hybridization Conditions for 32p-Labeled Probe, Protocol 5.6: Nonradioactive Biotin-Labeled Probes for Northern Blots

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