HONOUR THESIS A PILOT STUDY OF THE PREVALENCE OF COELIAC DISEASE IN VIETNAMESE PATIENTS WITH GRAVES’ DISEASE Khoa Dang Truong, BBMed Sci 207624 Supervisors Professor Peter R Ebeling, MD, MBBS, FRACP Dr Alex Boussioutas, MBBS, PhD, FRACP Dr Bob Anderson, PhD, FRACP Thesis submitted to University of Melbourne for the Degree of Bachelor of Science with Honours Department of Medicine (RMH/WH) Monday 27th October 2008 Word Count: 8,969 DEDICATION ~ This Honours Research Project and Thesis is dedicated to my Parents ~ In remembrance of my grandfathers Truong Thuong and Le Duc Thanh, both of whom had passed away with cancers ~ Their memories will remain with me for as long as I live ~ DECLARATION – PAGE OF Department of Medicine (RMH/WH) DECLARATION BY SCHOLAR Khoa Dang Truong (Student’s Name) I, Certify that • The thesis comprises only my original work, except where indicated in the accompanying Acknowledgement statement * • The thesis conforms to the specifications outlined in the Honours Handbook Signature: _ Date: _27/10/2008 DECLARATION BY PRINCIPLE SUPERVISOR Khoa Dang Truong (Student’s Name) Thesis is a I confirm that the declaration above of true and fair representation of the student’s work Signature: _ 23/10/2008 Date: _ *Your Acknowledgement page must declare, as appropriate: The extent to which the student has used the work of others The contribution of the student to work carried out in collaboration with others A description of work submitted for any other qualification A description of work carried out prior to enrolment in Honours DECLARATION – PAGE OF Department of Medicine (RMH/WH) DECLARATION BY 2ND SUPERVISOR Khoa Dang Truong (Student’s Name) Thesis is a I confirm that the declaration above of true and fair representation of the student’s work Signature: _ Date: 24/10/2008 _ *Your Acknowledgement page must declare, as appropriate: The extent to which the student has used the work of others The contribution of the student to work carried out in collaboration with others A description of work submitted for any other qualification A description of work carried out prior to enrolment in Honours DECLARATION BY 3RD SUPERVISOR Khoa Dang Truong (Student’s Name) Thesis is a I confirm that the declaration above of true and fair representation of the student’s work Signature: _ Date: _ 27/10/2008 *Your Acknowledgement page must declare, as appropriate: The extent to which the student has used the work of others The contribution of the student to work carried out in collaboration with others A description of work submitted for any other qualification A description of work carried out prior to enrolment in Honours ACKNOWLEDGEMENTS “Trust in the LORD with all your heart and lean not on your own understanding; In all your ways acknowledge him, and he will make your paths straight.” ~ Proverbs 3:5-6 In reverence of the Lord God Almighty, I am eternally grateful for His providence, blessings and faithfulness in all things Let all glory be to God, forever and ever Amen To my supervisor, Professor Peter Ebeling, thank you for your gentle words, your understanding and compassion Your unfathomed wisdom had always remained a mystery to me, even to this day Thank you for giving me a chance to follow my dreams and to experience research that would benefit others To my co-supervisor, Dr Alex Boussioutas, thank you for your generous guidance, patience and lenience Thank you for reminding me the reason why I worked so hard to be where I am today You will always have my respect, both as a scientist and a man of his word To my co-supervisor, Dr Bob Anderson, thank you for your genuine advice, reassurance and unrivalled expertise You were always there for me when there was no one else to turn to I will always remember the motivational chats and the meetings over lunch with you To all three of you, thank you for making this the best year of my study-life To Dr Claudia Gagnon, thank you for the time you have spent, taking me through the ethics approval process and showing me the statistical analysis I can never repay you enough for your kind intentions, and for helping me along every step of the way in my Honours Project this year To Dr Anjali Haikerwal, thank you for your references regarding the ranges of Vitamin D deficiency in the project To Dr Leo Rando, thank you for your referrals of patients from Endocrinology clinic To Sandy Dupuis, thank you for building and managing the database of participants for this project Your wonderful ideas and constructive criticisms were necessary and influential in the arrangements of this project To Maria Bisignano, thank you for directing the blood tests at Pathology of Royal Melbourne Hospital You were so dedicated and thoughtful, which made everything so easy to overcome To Margaret Watson, thank you for organising the blood collection at Western Hospital Even though you were short on staff, you still maintained an easygoing attitude and tolerance towards the rapidly increasing number of participants who came for blood test To Cathy Phillips, thank you for standing up for me in times of need Your presence alone, at the Pathology office, had encouraged me to endure through all the tough times To all the nurses who helped with the blood collection, thank you for your impressive skills and the ability to work under pressure To Marian Croft, thank you for your suggestions regarding the blood tests and patient history To Sally Edmonds, thank you for your directions with the online clinical information system To Jia Wei Teo, thank you for the transport and storage of the frozen blood samples in this project To Vivien Li, thank you for your fluent writing skills that were used to edit my ethics approval forms To Lynette Kalms, thank you for general administration and receiving patient phone calls in my absence To Fiona Wylie, thank you for your encouragement and warm-hearted smiles It wouldn’t be the same without you around the department To Erin Wijaya Ng, thank you for the initial accommodations of the project I wish you all the best in your future career To my cousin, Nguyen Tuong Dinh, thank you for your reading of the daily radio advertisements on Stereo FM 97.4 Vietnamese Radio Your specialty in radio advertisement had predisposed the potential participant to participation To Mr Thai Hoa and Mrs Phuong Hoang from SBS Vietnamese Radio 122.4 AM, thank you for the valuable time of your interview and daily advertisement broadcasts It was terrific to have you communicated our project to the broader Vietnamese Community To Dr Hai Phan, from the local clinics, thank you for your referral of participants and offer to promote our project to the Vietnamese Medical society To the Vietnamese Churches and Organisations, thank you for your overwhelming support for this project To my Honours friends and fellows DoMSA Committee who have travelled with me on this journey, thank you for your companionship and your support in my role as Honours Student Representative this year in 2008 I will never forget the precious time spent together on our Ski Trip, rock climbing, soccer games, and trivia nights Thank you all for being part of my life To my grandmother, Nguyet Thi Le, thank you for praying to God with tears for me To aunt Dung Thi My Truong, thank you for your enthusiasm in contacting friends and neighbours To my Father, Luu Vinh Truong; my mother, Hoa Kim Le; and my beloved brothers, Danh Cong Truong, Long Kim Truong and Thomas An Thien Truong Thank you You are the reason why I am here To all Vietnamese participants whose participation had made this Project possible… Thank you! TABLE OF CONTENTS TITLE PAGE DEDICATION DECLARATION PAGE OF 2……………………………………….………………………………………….3 PAGE OF 2…………………………………………….…………………………………….4 ACKNOWLEDGEMENTS TABLE OF CONTENTS ABSTRACT 10 ABBREVIATIONS 12 INTRODUCTION 14 1.1 GRAVES’ DISEASE…………………………………………………………………………… 14 1.1.1 Graves’ disease as a Cause of Hyperthyroidism……………………………………… 14 1.1.2 The Aetiology of Graves’ disease – Role of Autoimmunity………………………… 14 1.1.3 Genetic Background…………………………………………………………………….15 1.1.4 Prevalence of Graves’ disease………………………………………………………….16 1.1.5 Diagnosis of Graves’ disease………………………………………………………… 16 1.1.6 Current Therapy and Treatment……………………………………………………… 17 1.2 COELIAC DISEASE…………………………………………………………………………… 17 1.2.1 Coeliac disease as an Autoimmune Disorder………………………………………… 17 1.2.2 The Aetiology of coeliac disease – Disease Mechanism and Cause………………… 17 1.2.3 Genetic Background…………………………………………………………………….19 1.2.4 Prevalence of coeliac disease………………………………………………………… 20 1.2.5 Symptoms and Complications………………………………………………………….20 1.2.6 Diagnosis and Screening……………………………………………………………… 21 1.2.7 Current Therapy and Treatment……………………………………………………… 22 1.2.8 Consequences of untreated coeliac disease…………………………………………… 23 1.3 RATIONALE…………………………………………………………………………………… 24 1.3.1 The Link between Graves’ and coeliac disease……………………………………… 24 1.3.2 High rate of Vietnamese Graves’ patients at Western Hospital……………………… 24 1.3.3 Early Prevention reduces Morbidity, Mortality and other Risks……………………….25 1.3.4 Micronutrient Deficiencies Might Predict coeliac disease…………………………… 25 1.3.5 To Establish the Prevalence of coeliac disease in Vietnamese…………………………25 AIMS 26 2.1 Hypothesis…………………………………………………………………………………………26 2.2 Primary Aim……………………………………………………………………………………….26 2.3 Secondary Aim…………………………………………………………………………………….26 METHODS 27 Ethics Approval……………………………………………………………………………………… 27 3.1 PROJECT DESIGN……………………………………………………………………………….27 3.1.1 Study Population……………………………………………………………………… 27 3.1.2 Recruitment Strategies………………………………………………………………….27 3.1.3 Inclusion Criteria……………………………………………………………………….31 3.1.4 Exclusion Criteria………………………………………………………………………31 3.1.5 Database Establishment……………………………………………………………… 32 3.2 PROCEDURES……………………………………………………………………………………32 3.2.1 Initial Screening……………………………………………………………………… 32 3.2.2 Study Visit…………………………………………………………………………… 32 3.2.3 Biochemical Blood Tests……………………………………………………………….32 3.2.4 Genes Test and Small Bowel Biopsy………………………………………………… 33 3.3 STATISTICAL ANALYSIS…………………………………………………………………… 33 3.3.1 Sample size and Selection………………………………………………………………33 3.3.2 SPSS Software Analysis……………………………………………………………… 33 3.3.3 Limitations…………………………………………………………………………… 34 3.3.4 Potential Source of Bias……………………………………………………………… 34 RESULTS 35 4.1 Recruitment of Participants……………………………………………………………………… 35 4.2 Biochemical Analysis…………………………………………………………………………… 35 TABLE I: Baseline Characteristics of the Participants……………………………………….35 TABLE II: Prevalence of coeliac disease by Group………………………………………….36 TABLE III: Prevalence of Vitamin D Deficiency and Insufficiency by Group…………… 36 FIGURE 1: Prevalence of Severe, Moderate and Mild Vitamin D Deficiency and Insufficiency by Group……………………………………………………………………….37 TABLE IV: Prevalence of other Nutrient Deficiencies by Group……………………………37 FIGURE 2: Prevalence of other Nutrient Deficiencies by Group……………………………38 TABLE V: Prevalence of Anaemia, Microcytosis and Macrocytosis by Group…………… 38 FIGURE 3: Prevalence of Anaemia, Microcytosis and Macrocytosis by Group…………….39 DISCUSSION 40 5.1 Baseline Characteristics………………………………………………………………………… 40 5.2 Prevalence of coeliac disease in Vietnamese Graves’ disease Patients………………………… 40 5.3 Vitamin D Deficiency…………………………………………………………………………… 41 5.4 Anaemia in Graves’ disease……………………………………………………………………….42 5.5 Limitations Encountered………………………………………………………………………… 42 5.6 Source of Bias…………………………………………………………………………………… 42 CONCLUSION 44 REFERENCES 45 APPENDIX i APPENDIX 1: PARTICIPANT INFORMATION AND CONSENT FORM English Version…………………………………………………………………………………i Consent Form………………………………………………………………………………….vi Revocation of Consent Form…………………………………………………………………vii APPENDIX 2: PARTICIPANT INFORMATION AND CONSENT FORM Vietnamese Version………………………………………………………………………….viii Consent Form……………………………………………………………………………… xiii Revocation of Consent Form……………………………………………………………… xiv APPENDIX 3: PARTICIPANT QUESTIONNAIRES – English Version Patient Questionnaires……………………………………………………………………… xv SF36 Health Survey…………………………………………………………………………xvii APPENDIX 4: PARTICIPANT QUESTIONNAIRES – Vietnamese Version Patient Questionnaires………………………………………………………………………xxii SF36 Health Survey……………………………………………………………………… xxiv APPENDIX 5: INVITATION LETTER TO PARTICIPANTS – English Version……………… xxix APPENDIX 6: INVITATION LETTER TO PARTICIPANTS – Vietnamese Version…………….xxx APPENDIX 7: ADVERTISEMENT POSTER – English Version…………………………………xxxi APPENDIX 8: ADVERTISEMENT POSTER – Vietnamese Version…………………………….xxxii APPENDIX 9: DE-IDENTIFIED CODE KEY………………………………………………… xxxiii APPENDIX 10: PATHOLOGY REFERENCES………………………………………………….xxxiv APPENDIX 11: PATIENT QUESTIONNAIRE ANSWERS PART OF 3………………………………………………………………………………xxxv PART OF 3…………………………………………………………………………… xxxix PART OF 3……………………………………………………………………………… xliii APPENDIX 12: SF36 HEALTH SURVEY ANSWERS PART OF 2……………………………………………………………………………….xlvii PART OF 2………………………………………………………………………………… li APPENDIX 13: PARTICIPANT BLOOD TEST RESULTS PART OF 2………………………………………………………………………………….lv PART OF 2…………………………………………………………………………………lix ABSTRACT Current data suggest that the prevalence of coeliac disease is high in Caucasian with Graves’ disease However, there are no data assessing the prevalence of coeliac disease in Vietnamese with Graves’ disease; nor data regarding micronutrient deficiency in Graves’ disease patients, and whether this is higher compared with the normal Vietnamese population In this study, we aimed to establish the prevalence of coeliac disease in Vietnamese patients with Graves’ disease The prevalence was compared with Vietnamese participants without autoimmune disease, and also with the prevalence in Caucasians as previously reported in the literature Furthermore, the frequency of micronutrient deficiency was determined in Vietnamese patients with Graves’ disease and in control participants The population of participants included 60 Vietnamese Graves’ disease patients and 120 Vietnamese control participants, who were recruited using various recruitment strategies The most successful strategy involved Radio Advertisement and a national-broadcasted interview on two wellknown Vietnamese radio stations, Stereo FM 97.4 Vietnamese Radio and SBS Vietnamese Radio 122.4 AM Consequently, the outcome of recruitment has shown an effective study design needs to overcome the underlying cultural misconceptions and implications, to investigate a range of useful methods that successfully accessed the whole Vietnamese community Altogether, 180 Vietnamese participants were invited, who gave consent to answer two lifestyle questionnaires, and underwent a collection of blood sample for pathology testing The results were organised into a database and statistically analysed, using different combinations of unpaired Student t-test, Mann Whitney test, Pearson’s Chi-square and Fisher’s exact test Coeliac disease was diagnosed using IgA anti-tTGA test in Vietnamese Graves’ disease patient and Vietnamese control participants (excluding with borderline result) Thus, the calculated prevalence of coeliac disease in Vietnamese Graves’ patients is 1:60, which is similar to Vietnamese control participants; but likely to be lower, compared to 1:22 prevalence of coeliac disease found in Caucasian Graves’ disease patients On the other hand, we observed a high prevalence of Vitamin D deficiency in the Vietnamese participants of our pilot study The rate of Vitamin D deficiency and insufficiency was found to be 10 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient ECFM019410101 ECFM023460916 ECFM032440921 ECFM068400601 ECGF010280121 ECGF050310504 ECGF051300617 ECGF056271010 ECGF060350227 ECGF072360520 ECGF093370807 ECGM057280214 ECGM092350403 ECHM059270202 ECHM070251212 WPAF005890529 WPAF215871129 WPBM106821020 WPBM154850421 WPCF079731022 WPCF087690628 WPCF088710527 WPCF096730219 WPCF097710514 WPCF111750723 WPCF125690818 WPCF130720428 WPCF131711012 WPCF142690213 WPCF158720102 WPCF166700401 WPCF168721214 WPCF188680730 WPCF211680913 WPCF218690915 WPCF221721107 WPCM224701109 WPDF086670104 WPDF089670308 WPDF104581011 WPDF108600102 WPDF112620503 WPDF113580220 WPDF128601110 WPDF132570929 WPDF133661021 WPDF153610705 WPDF155600926 WPDF167650107 WPDF174651216 WPDF205581031 WPDF208620905 WPDF214610711 WPDF217670209 B C C B D B C D E E D D C D C C C D B D D C D D D C D D D D B C D A C C D C A D D D D D D D D D D C E D C E C C D D C B A A D C C D B D D B C D A D B C D E D C D B B D C C C A C D C D D B C D D D D A C D C D D C D E B B C B B A A A A A B A B A B C C B C B B C B A A C A C C B B C C B B B C A B B A B B A A C C B A A C C C A C C C B A A B B A A B A B B A C C B C B B C B C A C A C C B C C C C C C C B B B B B C B A C C C B B C C C A C C C B B B B B A B B C B B A C C B C C B C B C A C A C B A C C B C C C C A B A B B C B A C C C C B C C C A xlix C C C B B B B B A B C B C B B C C B C B C C B B A C A C C C C C C C B C C B B A B B B B A C C B C B C C C A C C C B B B B B A B C C C B C C C B C C C C B C A C A C C C C C C C C C C C C B C C C B B C C C C C C C C A C A C B B C B B A A B C B B B C B B C B C B B A A C A C C A C C B C C C B B C B B B B C A C C C C B C C C A C C C A B B B B A A B B B A C C C C C B B C B A A C A C C C C C A C B C C C B A C B C A B C C C C C C C C A C C C B A B B B A B B C C A C C C C C C B C B C B C A C C C C C C C B C C C C A C B C A A C C C C C C C C B C C C C A B B B A C B C C B C C C C C C C C B C C C A C C C C C C C C C C C C B C B C B B C C C C C C C C C C C C B C B C A B B C C C B C C C C C C C C B C C C B C C C C C C C C C C C C C C C C C A C C C C C C C C C N N N Y N N Y N Y N Y N Y Y N N N Y N Y N N N Y Y N Y N N N N N N N N Y N N N N N Y N Y Y N N N N N Y N N Y N N N Y N N Y N Y Y Y N Y Y N N Y Y N Y N N Y Y Y N Y N Y N N N N N N Y N N N Y Y Y N Y Y N N N N Y Y N N Y N N N Y N N Y N Y N Y N Y Y N N Y Y N Y N N Y Y Y N Y N N N N N N N N Y N N N Y Y Y N Y Y N N N N N Y N N Y N N N Y N N Y N Y N Y Y Y Y Y N N N N Y N N Y Y Y N Y N N N N N N N N Y N N N Y Y Y N Y Y N N N N Y Y N N Y 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient WPDF219670619 WPDF222660105 WPDM083650219 WPDM220601220 WPEF012560606 WPEF078511220 WPEF105570423 WPEF163540703 WPEF172521202 WPEF186520510 WPEF196560207 WPEF202480920 WPEF213520310 WPEF216491007 WPEM141561220 WPEM212500120 WPFF191450626 WPFF223440517 WPFM126450709 WPFM194421207 WPGF004331212 C D D D D D C D D D C C E D D C D C D C D D C D C D B B D B C D C E B D D C B D B B B C B B B B B C A B A A A C B B A A C A A C C C C B C B C B B C B A C B C B C C C B C C C C B C B B B A B B A C A B B B C C A l C C C C B C B C B B B B A C C B B B B C A C C C C B C C C B B C B B C C C C C C C A B C C B B C C C A B B A A C B B B B A B B B C D C B C B C C A B B A C B C A C C C A C C D C B C B C B A C C B C C C C C C C A C C D C C C C C B B C C B C C C C C C C B C C D C B C C C C C C C B C C C C C C C C N N N N N Y N N Y N N N Y N N N Y N N N Y N N Y Y Y Y N N Y N N Y Y N N N Y N N N Y Y N N Y Y N N N Y N N Y Y N N N Y Y N N Y N N N Y Y N N N Y N N Y Y N N Y N N N N Y APPENDIX 12: SF36 HEALTH SURVEY ANSWERS – PART OF No 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 Group Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control 5a N N N N N N N N N N N N N Y N N N N N N Y N N N N N N N N N N N Y N Y N N N N N Y N N N Y N Y N N N N 5b N N N N Y N N N N N N N N Y N N N N N N Y N N N N N N N N N N Y Y N Y N N N N N Y N N N Y N Y N N N N 5c N N N N Y N N N N N Y N N Y N N N N N N N N Y Y N N N N N N N Y N N N N N N Y N Y N N N N N Y N N N N B B B B B B A A A A B A A C A B A A A B A A B A A A A A A A A B A A A A C A B A B A A A B A D A A A A A B A A A B A A A B C B A B A B A A A B B A C A A A A A A A A B A B A B D A A A C A A A C A E A A C A A A A A A A A A A A C A A B A B A A A B B A C A A A A A A A A B A B A B C B B A B A A B C A E A A A A 9a E B D C D D B A B B D E F B E F B C C C C D C B B D B A C B B C B B A C E A C C D A F A D A E B A B B 9b A E F E B C E E C E F F E D E F E D C C E A C D C D F D F C C B E D C C A E B C D D F D C B B C E A C 9c D E E E D E E F F F F F F E D F F E E E C D D B F F E E B F F C E D E D B E E D D E F E D C C F F E F li 9d C B B D E C B A B A A B A C E B C A B B B D B A B C B B B B B D B B B D F A B C D B A A E A D B A D B 9e D B B B E D B A A B D B A C E C C D B C B D B B A C B B A A A D A C C D F A B C D A A A C A D A B A A 9f E E F D D F E E F F F F F D E F E E E E D D B E F F F E F F F C F C F D B E F D D E F D C C A F E D F 9g D F C D D E D D F E D F E C E D D D E E E F D F F D E F F F F C C D E E B E F F D F F F C C A F F D F 9h D B B C B D B A B A B B A C D B C B B B C C C E B B B A B B B D C B C A E A A C B B A A D A D B A A B 9i C E D E B D E D E D D D E B E D D D E D D D E D E D E E E E E C C C E D B E C C C E F D C C A F E D E 10 D D E D D D D E D D E E E C C D E E D D D E C D D D E D D D D C E C D C C E E B C E E D C C A E D C D 11a B E E B C C D B E D B E C C C B E C D C E C B E E C C C B E E C C C C C B E E D D C E E C E A E C E E 11b C D C D B B C B B A D B B D C B A B B C A B D C B C B C B B B C C B B B D A B A C B A A C B E B B B B 11c C C D D C C C D D C C E C B C E D B C C C C A C D A C C D D D B C C E B B E B D C B A C B C A E C B D 11d C D B C C C C C B C C B C E C B B C C C C C C C B B C C B B B D C C D C D B C B C C B C D A E B B B B 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control N N N N N Y Y N Y N N Y N N Y N N N N N N N N N N N N N N N N N Y N N N N N Y N Y N N N N Y N N N N N N Y N N N N N N N Y Y N N N Y N N Y Y Y N N N N N N N N N N N N N N N Y N N Y N N Y N Y Y Y N Y Y N N N Y Y N Y N N N N N N Y Y Y N N N Y N N N N N N N N N N N N N N N N N N N N Y N N N N Y N N N Y N N Y Y N N N Y N N N N A A A A A C A A B A B C B A B B B A A A A A A A A A A A A A A B C B A A A A A A B C A A C B A A D B A B C A A B A B B D B B B A B C B A B C B A A A B A A A B A B B B A A B C C A B A A A A B C A A C C A C D B B B E D A B A B B B A B B A B C B A B B C A A A B A A A A A B B B A A B C D A B A A B A B C A A C B A A D B B B D B B B D D B A C B D B D C B F D B B B D B D A A B B B C C C B D C D D B B D B A B D D A B E D B F B E C B B E C E D C D C D C B E D C E F D C E C D E D E D E C E F D D E E D D D F D E D D F D D B E B B C F B D C E A C F F D C E C E E E F D E E F D C E F F F F F F E F F F D D E F C D D F D E B D F F E F F B D F D B F E F D F lii B B B D B A B B B B C B A A B B B A C B F A B B B A B C C B A C D C B C B A C B B A B B E D B B E E C B B B A B B C B A B B B B C C B E A B B B C B A A A C B A B C C C D B E B B E B B A B E C B A C C A B E E C B C C F F D C E F F F D F E D E E D D E F E F F F F E E F F D D E F D D C F D D E D F E D F E B D F B C E D F D E F F D E D C F E E F E E E F D C C F E E F F F E E E E E E F E D D E F E E F D F E D D F C B F E D E E E C D B D B F B A B D B B B B A A A C B A D B A A B B B A B B B B D D C D B D B E A A C B B B D D B B D A A B B C E E D E D C E E F E D D C E D D C F C D C F F E E E D D D D E D D B E E E D D F C C E E D A E E B D D C B C D D E E D C C C D D D D D D C D D E C E C E F D E D C D D D D C C D E C E D C D C B E E C C D A B C D C C D E D B B C C C C C D B C C E E C D C C C C D D C C C C C C C B C C C E B E E C C C C D E D B E C C D C C B C B C B C B C C C C B C C C A A C B B B B C B B C B C C C C C B C C C B C A C C E C C B B D B B C D C C C D C D B B B B C C C C D C B C E A B B D C C C D D D D B C B B B D C A C D B C C C C C B B E B B D C A B B C B B B D D D D C C C C B C C C C B C D B C C C B B B C D C E E C B C C C C D C C C A C C B B D D B C D C E C B D 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient N N N Y N N Y N Y N Y Y Y Y N N N N N Y N N Y N Y N Y N N N N N N N N Y N N N Y N Y N Y Y N N N N N Y N N Y N N N Y N N Y N Y Y Y Y Y Y N N N N N Y N N Y N Y N Y N Y N N N N N N Y N N N Y Y Y N Y Y N N N N Y Y N N Y N N N Y N N N N Y N Y Y Y Y Y N N Y N Y N N Y Y Y N Y N N N N N N N N Y N N N Y N Y N Y Y N N N N N Y N N Y A A A D B B B B B A C B C C A A A B B C A A B A B A D B A A A A A A A C A A B A B D A D B A A A A A B B A D B A A B D D B B D B C B C C A B A A B D A A B E D A D A A D A B A B A D A B D A B C B D B A A B B C D B B D A A B B B C B B D D C B C D A A A A A C A A B D B A D A A D A A A A A D A B D C B C B C B A A A B B E C A D B B B B B B A B E C D D D C D B D D D E B C E C E D E E D D A B B D B E A C D D D D D B E D A D D D E C F F D C E E D F E E B D D D D C E B E E C D C C D E D A A D B D F F E E D D E E D E C B B D B B E E C B D D F A F F F E C F E E B B D E D D F E F C E D F D D F D B A E B E F F E F E D E E D E E C B E B D F F C B D E F A liii B B B B B B A A E C C B C B A B B E B B B C D C D C B C E C A B B C B E A B C D C D C B E B A C C C D B F B A A B B C B B B E D D B D C B B C D B C B C C D D C E D E D A B C D B D A C E D E D E B C D B C C C D B F F F F F D E F E E A D C E C D F E D C E D E D D E D D A E B E F F F F D D E E D E D C B E B E E F C B F E F A E F E D E F F F E C B C D D E E E C E D E E D E C D A D D E F F E F F D D D B D F B C C C C F F D C D D F D B B B B B A A B E B D B D B A B B C B C B B C C D C E C D C A B D B B C A B D C D D E B D B B B D C D B A F E E F D D C E E B B D C D C E D E C E B D D B C D D A D C C E E E D D B D D A D D B B D B C E D C B A D D A E D E C C A C D D B C B C C D E D B D C D D C C C C D E C D E D A E D C D D C C D C E B B C A E C C C E E B D E D D C C C C C B C C C C E C C C D B B C B B B C B C B C E C E E C B B B C C C C A C B C B C C C A C C A B B C C C B C C C D C C C C A B C D B C C B D D E C D C D C B B E C B C D D C C B D E C D B C C B B C C C E D D B B D D C C C B B B B B D D E C D B C B B A B C B B B B E C A E C B B D B B B B B C A B C C C B B B C A A B C D C B C C C D D C D D B D E D B D C C D E E C D B C C C B D C C D B C C C C E D C E D E C C C D C C E 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Y N N Y N Y N N Y N N Y Y N N N Y N Y N Y Y N Y Y N Y N N Y N Y Y Y N N N Y N Y N Y N N N Y N Y N N Y N N Y Y N N N N N Y N Y A A A A A B A A B D B B D A A B A A C A B A B B B B B A B B D B B D B B B C A C A C A A B B B B A A B C B B D B B A B A C A B B D B C E B B B C E D C E E D E C B D B D D A D D D D D F A B B C A A C D E E F E B F C F C D D E F B B D D B E F D E F F D B liv B C B C C B B B D C D C E A C D A B C B E B D B C D B B B C D B C F A C C C B B B C E C F E B E E F C B D C A A D D F F F E C F D D D C D E F C B D C A E C E F F F F E B D B A D B B A B E B B E C A B B B B B D D D C C C D D F B B D C B C C E E D D D D D D C C D D D E D D D C B E C D C E E C C C C D B B B D E C C C C A C C D D C E C C B C C D C C B B C C B B E B C B B B B B C B C B C C B C B C C D B A C C D C C B C B C C C D C C C C C E D C E C C B C C D C D APPENDIX 13: PARTICIPANT BLOOD TEST RESULTS – PART OF No 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 Group Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Participant No ECAF044881215 ECAF045880906 ECAF046880717 ECAF047881011 ECAF067890313 ECAM048881129 ECAM049881024 ECBF008870419 ECBF026840820 ECBF042791123 ECBF043831117 ECBF071790818 ECBF074780324 ECBF082851009 ECBF085770905 ECBF095820302 ECBF098870402 ECBF123831220 ECBF124850318 ECBF145800212 ECBF147790418 ECBF201800602 ECCF017730331 ECCF022690731 ECCF028720922 ECCF029671119 ECCF030701029 ECCF033740517 ECCF035730305 ECCF036731024 ECCF039731207 ECCF090681113 ECCF102720122 ECCF107681130 ECCF140680501 ECCF149690625 ECCF150681002 ECCF162730905 ECCF176760107 ECCF183760917 ECCF198701011 ECCF207690904 ECCM025740508 ECCM041730830 ECCM075710331 ECCM080690308 ECCM084690218 ECDF002591229 ECDF007600630 ECDF015640304 ECDF020620411 Hb 129 134 129 155 138 151 157 136 139 136 140 133 136 133 138 119 120 130 126 135 130 116 127 144 138 126 141 143 138 127 143 136 126 121 144 131 133 130 139 155 138 148 138 160 148 142 135 116 133 131 106 WCC 8.2 9.6 7.3 6.9 6.7 6.7 8.3 8.1 8.2 6.9 7.8 7.9 8.3 7.2 8.2 7.8 7.6 7.9 8.8 7.7 9.4 6.9 8.7 7.3 7.1 7.9 8.7 7.1 7.5 7.4 7.1 7.2 8.1 7.4 6.9 7.2 6.7 7.9 8.6 7.5 6.9 8.4 7.8 7.7 11.4 7.4 6.8 6.8 PLT 187 355 262 262 273 435 401 269 216 289 239 263 298 323 235 221 324 319 207 204 176 227 192 314 209 228 252 217 153 316 199 256 308 211 233 309 314 244 259 247 200 236 285 226 236 196 358 352 308 327 380 lv RCC 4.24 5.16 4.29 5.17 4.89 4.85 5.4 4.47 4.47 4.59 4.62 4.44 4.42 4.34 4.58 4.01 4.9 4.16 4.02 4.23 4.19 3.87 4.23 4.99 4.59 4.09 4.71 4.59 4.61 4.01 4.72 4.46 4.03 3.66 4.96 4.27 4.46 4.49 4.54 4.92 4.34 4.73 4.45 5.49 4.75 4.7 4.53 4.48 4.23 3.94 PCV 0.37 0.4 0.38 0.44 0.42 0.44 0.45 0.4 0.41 0.4 0.42 0.39 0.39 0.39 0.41 0.35 0.37 0.39 0.37 0.39 0.36 0.34 0.38 0.43 0.41 0.38 0.41 0.41 0.4 0.38 0.42 0.4 0.38 0.36 0.42 0.39 0.4 0.38 0.41 0.45 0.39 0.43 0.41 0.46 0.43 0.42 0.4 0.36 0.39 0.38 0.32 MCV 87.8 76.5 88.8 85.1 85.5 90.4 83.4 88.4 92.2 87.5 91.4 87.8 88.6 90.3 88.4 87.7 76.2 93.3 93 93.3 86.4 86.9 89 85.8 89.5 93 87.7 90.4 86.3 93.7 88.3 90.3 93.6 96.9 85.4 91.3 88.8 85.1 91.1 91.9 90.8 90 91.8 84.7 86.9 88.1 84.8 78.8 87.1 90.7 81.7 MCH 30.5 26 30.2 30 28.3 31.1 29.1 30.5 31 29.6 30.4 29.8 30.9 30.6 30.2 29.7 24.5 31.2 31.5 31.9 31.1 29.9 30 28.8 30 30.9 29.8 31.2 29.8 31.6 30.3 30.6 31.4 32.9 29 30.8 29.8 29 30.6 31.5 31.7 31.2 31 29.2 29.6 29.8 28.7 25.6 29.7 31.1 27 MCHC 348 339 339 353 331 344 349 345 337 338 332 340 348 338 342 339 321 335 339 342 359 343 338 336 336 332 340 345 346 337 343 339 335 340 339 337 336 341 336 343 350 347 337 345 341 338 339 325 340 343 330 RDW 13 13 12.2 11.3 13.2 11.9 12.2 11.8 11.5 14 12.4 12.4 12.5 13 12.3 12.8 15.1 12.7 12.4 12.6 11.5 13.3 13.7 12.9 12 13.4 12.7 12.3 11.4 12 13.1 13.2 12.9 13 12.2 12.5 12.6 12.1 14 12.4 12.1 12.4 11.2 12.5 12.6 12.4 12.7 28.5 12.8 11.9 13.9 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control ECDF034611002 ECDF063611018 ECDF065660725 ECDF069640408 ECDF077640512 ECDF100620307 ECDF101630422 ECDF109630608 ECDF110630119 ECDF116580703 ECDF118620225 ECDF137640222 ECDF177640322 ECDM006570809 ECDM014610427 ECDM016660620 ECDM076570810 ECDM081610810 ECDM091670606 ECDM094621118 ECDM099631120 ECDM103650325 ECDM115581205 ECEF003550104 ECEF018520527 ECEF027550731 ECEF038560708 ECEF052521012 ECEF053521220 ECEF061470920 ECEF073541008 ECEF120560509 ECEF121560122 ECEF170551014 ECEM001521028 ECEM009570511 ECEM011500828 ECEM021570106 ECEM037500721 ECEM040550802 ECEM064521225 ECEM114490429 ECEM117560810 ECEM119520810 ECEM127561114 ECFF013381225 ECFF024460820 ECFF031460810 ECFF054451010 ECFF055441020 ECFF058460204 ECFF062430109 ECFF066380925 ECFF122470626 118 128 130 119 135 130 137 128 143 136 131 139 135 141 149 168 139 143 131 162 152 142 161 99 127 120 140 135 136 126 149 117 135 129 146 146 158 139 134 159 144 151 158 135 132 120 131 127 120 123 119 134 139 129 7.8 7.8 8.2 9.2 7.9 7.6 7.3 6.6 9.4 6.8 7.3 7.7 7.8 7.5 8.4 8.1 8.1 7.4 8.5 6.8 7.4 7.7 7.9 6.8 7.7 7.7 8.6 7.4 7.6 7.5 7.1 7.7 7.2 7.8 7.6 7.9 8.2 7.4 7.4 6.9 7.3 7.7 6.7 7.3 7.3 7.2 6.4 9.4 7.7 6.7 8.4 336 237 235 248 204 209 326 240 213 244 267 220 278 167 283 195 457 217 239 214 276 239 171 242 328 234 226 341 377 305 351 334 254 297 272 387 256 352 246 182 262 252 180 296 250 403 259 291 255 271 286 193 300 186 lvi 4.57 4.34 4.32 3.83 4.23 4.27 4.54 3.96 4.47 4.07 4.41 4.36 4.11 4.55 5.03 5.57 4.59 4.93 4.38 5.17 5.02 4.66 4.95 4.84 4.12 4.23 4.71 4.57 4.26 4.5 4.88 3.77 4.64 4.27 5.13 4.89 5.35 4.68 4.44 5.18 4.65 5.01 4.91 4.72 4.34 3.8 4.25 4.17 3.98 3.98 3.92 4.33 4.62 4.07 0.36 0.38 0.39 0.35 0.4 0.38 0.4 0.38 0.41 0.39 0.38 0.4 0.38 0.41 0.45 0.5 0.42 0.43 0.39 0.47 0.44 0.42 0.47 0.31 0.38 0.36 0.41 0.4 0.39 0.38 0.43 0.33 0.4 0.38 0.43 0.44 0.47 0.42 0.39 0.47 0.43 0.46 0.46 0.41 0.39 0.36 0.4 0.38 0.34 0.36 0.36 0.39 0.4 0.38 78.6 88.1 91 90.4 93.3 88.1 88.9 95.3 92.3 95.7 86.8 92.8 93.7 90.8 89.7 89.4 90.8 86.7 89.1 90.2 87.5 89.3 95.1 64.6 92.2 85.3 87.2 88.4 92.4 83.3 88.5 88.1 86.7 88 84.6 90.4 87.9 89.6 88.1 91.4 92.8 91.4 94.5 85.9 89.1 93.7 93.6 90 86.3 90.7 90.6 91 87.5 94.3 25.9 29.5 30 31.2 31.9 30.4 30.1 32.2 31.9 33.5 29.7 31.8 32.8 31 29.6 30.1 30.2 29 29.9 31.4 30.3 30.4 32.5 20.5 30.8 28.4 29.7 29.5 31.9 27.9 30.5 31 29.1 30.1 28.4 29.8 29.5 29.6 30.1 30.7 30.9 30.2 32.3 28.5 30.4 31.6 30.7 30.4 30.1 30.9 30.3 31 30.1 31.7 329 335 330 345 342 345 338 338 346 351 342 343 350 342 330 337 332 335 336 348 346 341 341 317 334 333 341 334 346 335 344 351 335 342 335 329 336 331 342 336 333 331 342 332 342 337 328 338 349 340 335 341 344 336 14 12.9 12.9 15.3 12.6 12.4 14.3 13.3 14 12.8 12.5 12.4 12.2 12.5 13.4 13.2 12.6 13.6 14.7 13 13 13.8 13.1 15.6 12.9 13.3 11.9 13.4 13.2 13.7 12 11.9 13.6 12.7 13.9 14 13.9 13.4 12.3 13.3 11.9 13.2 13 13.5 12.3 12.4 12.6 14.3 12.5 12.7 13.2 12.1 12.2 13 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient ECFM019410101 ECFM023460916 ECFM032440921 ECFM068400601 ECGF010280121 ECGF050310504 ECGF051300617 ECGF056271010 ECGF060350227 ECGF072360520 ECGF093370807 ECGM057280214 ECGM092350403 ECHM059270202 ECHM070251212 WPAF005890529 WPAF215871129 WPBM106821020 WPBM154850421 WPCF079731022 WPCF087690628 WPCF088710527 WPCF096730219 WPCF097710514 WPCF111750723 WPCF125690818 WPCF130720428 WPCF131711012 WPCF142690213 WPCF158720102 WPCF166700401 WPCF168721214 WPCF188680730 WPCF211680913 WPCF218690915 WPCF221721107 WPCM224701109 WPDF086670104 WPDF089670308 WPDF104581011 WPDF108600102 WPDF112620503 WPDF113580220 WPDF128601110 WPDF132570929 WPDF133661021 WPDF153610705 WPDF155600926 WPDF167650107 WPDF174651216 WPDF205581031 WPDF208620905 WPDF214610711 WPDF217670209 137 153 158 151 108 143 137 135 135 122 127 127 153 113 122 139 127 153 144 127 146 129 128 130 133 129 132 137 138 130 114 132 128 132 120 128 147 125 138 131 141 125 111 124 150 145 116 128 123 127 143 133 133 142 6.7 6.8 7.3 7.6 6.9 8.1 6.1 6.9 7.1 7.8 6.3 6.9 7.8 8.8 7.2 8.2 8.3 7.4 6.9 7.4 7.2 8.1 6.6 6.3 7.1 8.7 7.8 7.9 7.2 7.1 7.7 7.4 7.7 7.4 7.6 6.8 6.9 7.6 9.2 8.7 8.3 7.2 5.9 8.3 6.9 7.1 6.9 6.5 7.2 7.1 323 162 233 308 269 297 287 389 300 314 304 195 256 286 170 257 260 248 210 229 284 338 247 316 252 220 361 229 225 202 275 291 200 217 307 307 223 206 344 298 266 191 116 250 265 340 268 323 248 370 279 209 274 266 lvii 4.75 4.82 5.05 4.73 3.86 4.78 4.55 4.35 4.62 4.01 4.07 4.61 4.74 3.15 3.71 4.37 4.62 5.45 4.62 4.22 4.79 4.5 4.05 4.25 4.84 4.28 4.65 4.32 4.67 4.26 3.89 4.45 4.29 4.42 4.07 4.8 4.07 4.95 4.34 4.24 4.37 5.8 4.54 5.22 4.84 5.53 4.12 4.09 4.24 4.57 4.29 4.26 4.7 0.41 0.45 0.46 0.45 0.32 0.42 0.41 0.4 0.41 0.36 0.38 0.39 0.46 0.33 0.36 0.41 0.38 0.46 0.42 0.37 0.42 0.38 0.37 0.38 0.4 0.38 0.38 0.4 0.41 0.39 0.34 0.38 0.37 0.39 0.36 0.37 0.43 0.36 0.42 0.38 0.41 0.37 0.35 0.37 0.45 0.42 0.36 0.38 0.36 0.37 0.41 0.39 0.39 0.42 85.5 92.4 90.4 94.3 82.5 89 89 91.4 87.8 88.6 92.9 84.7 95.9 105.9 96 93.5 83.3 83.5 91.9 88.2 86.9 83.6 90.3 88.9 82.2 89.1 82.7 93.1 87.2 90.9 87.8 86.2 85.1 87.5 90.7 90.8 90.4 89.8 84.2 88.4 97.2 85.3 59.8 80.9 86.2 87.7 66 91.2 88.3 86.7 89.9 91.1 92.4 89.8 28.8 31.6 31.3 32 27.9 29.9 30.1 31 29.3 30.4 31.3 27.5 32.3 35.9 32.8 31.7 27.5 28.1 31.1 30 30.5 28.6 31.4 30.6 27.4 30.1 28.3 31.7 29.4 30.5 29.2 29.6 29.7 29.9 30.1 31.4 30.6 30.8 27.8 30.1 33.3 28.6 19.1 27.3 28.7 30 20.9 31.1 30.2 29.9 31.2 31 31.3 30.3 338 343 346 340 338 336 338 339 333 343 337 325 337 339 342 340 330 337 338 341 350 342 348 344 334 337 343 340 338 336 333 343 349 342 332 345 338 343 330 340 343 336 319 337 333 342 317 341 342 345 347 340 338 337 14.3 12.6 12.4 13.3 13.7 12.3 12.9 13.4 13.5 13 13.3 14.5 13.9 19.5 13.1 13.2 13.7 13.3 13.1 12.2 12.4 13.8 13.3 11.8 13.8 13.2 13.5 12.6 14.2 12.3 14 12.7 13.8 13.3 12.5 12.4 13.7 12.2 15.1 13.1 12.8 13.2 16.6 13.1 13.9 12.5 15 12.2 12.9 12.8 12 13.1 13.6 13.2 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient WPDF219670619 WPDF222660105 WPDM083650219 WPDM220601220 WPEF012560606 WPEF078511220 WPEF105570423 WPEF163540703 WPEF172521202 WPEF186520510 WPEF196560207 WPEF202480920 WPEF213520310 WPEF216491007 WPEM141561220 WPEM212500120 WPFF191450626 WPFF223440517 WPFM126450709 WPFM194421207 WPGF004331212 129 124 156 167 130 133 139 139 111 135 133 136 127 135 154 150 119 140 155 149 108 6.4 8.7 8.3 8.2 7.3 7.7 7.6 7.3 7.5 6.7 6.2 7.3 7.4 6.4 8.2 7.8 7.8 6.4 289 217 217 177 326 153 296 301 106 157 245 272 369 204 294 222 225 268 288 214 248 lviii 4.14 4.41 5.13 5.17 4.06 4.24 4.57 4.84 3.47 4.3 4.53 4.45 3.99 4.17 5.26 4.97 3.54 4.82 5.02 4.69 3.74 0.38 0.37 0.45 0.48 0.38 0.39 0.4 0.41 0.31 0.39 0.39 0.39 0.37 0.39 0.45 0.45 0.34 0.42 0.46 0.43 0.33 91 84.1 87.9 92.8 92.7 92.5 88.1 85.7 89 91.4 86.9 87.2 93.9 94.5 85 89.7 94.8 87.7 92 90.8 87.6 31.1 28.1 30.3 32.3 31.9 31.4 30.4 28.7 32 31.3 29.4 30.6 31.9 32.4 29.3 30.1 33.5 29 30.9 31.7 28.8 342 335 345 348 344 339 345 335 360 343 338 351 340 343 345 336 353 331 336 348 328 12.6 14.1 13.5 12.9 13.3 13.3 13.3 11.9 13.2 12.4 14 12.4 12.9 13.2 12.6 12.6 12.5 12.6 12.5 13.6 13.7 APPENDIX 13: PARTICIPANT BLOOD TEST RESULTS – PART OF No 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 Group Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control sFol 30.1 13.3 31.4 25.3 16.1 17.8 15.6 19 18.8 18.7 19.7 15.6 31 12 21.5 24.9 15.2 41 26.6 27.9 14.1 17.8 23.7 26 13.5 27.2 27.1 37.6 25.6 26.2 34.1 18.5 23.7 18.5 23.2 16.2 17.1 38.8 12.7 27.6 54 28 17.6 14.8 20.9 16 18.6 31.2 21.3 24.1 43 Fe 12 13.3 16.6 21 11.4 24.4 29.3 15.2 16.6 15 14.3 21.7 21.6 23.6 33.2 20.1 19.1 18.4 25.9 10.3 8.2 20.5 15.7 19.1 21.1 9.8 23.1 19.3 27.5 22.7 17.9 14.8 15.8 16.4 24.8 17.7 14 17.4 24.8 19.3 27.8 28.3 14.3 29.6 28.5 12.1 14.7 19.6 12.6 4.6 Na 138 139 137 139 138 140 139 137 138 138 140 138 138 138 138 135 137 136 138 136 140 140 136 138 139 141 139 139 136 139 138 139 137 138 140 142 143 140 141 137 143 138 140 140 137 138 143 140 137 136 140 K 4.1 3.8 3.7 3.9 3.7 3.8 3.7 3.6 3.8 3.8 3.9 3.5 3.5 4.1 3.5 3.7 4.1 3.6 3.5 4.1 3.9 4.1 4.2 3.9 4.3 4.1 3.8 3.8 3.7 3.8 4.1 3.6 4.3 3.8 3.8 4.8 3.5 3.9 3.7 4.2 3.8 4.4 3.7 3.9 Cl 104 102 101 104 103 102 103 105 101 104 103 104 104 104 105 103 103 101 104 104 106 108 105 103 102 107 104 104 105 103 104 106 104 104 105 108 107 104 105 100 108 104 105 103 106 105 105 105 104 102 106 Crea 90 70 70 80 80 80 80 70 70 60 80 60 60 80 60 60 70 70 70 70 70 60 70 70 60 60 80 60 70 70 60 60 60 70 70 80 80 60 60 70 80 60 90 100 80 80 80 60 60 60 90 Urea 6.6 3.6 3.4 5.5 4.8 4.3 7.4 5.7 6.9 4.7 4.7 3.4 4.1 2.4 3.8 4.3 4.1 5.9 3.3 4.6 3.8 4.4 3.5 4.5 2.7 5.2 4.9 3.6 4.9 4.6 3.3 4.8 4.5 4.2 4.9 3.9 5.3 6.6 7.1 7.6 4.8 4.3 4.5 3.8 lix Ca 2.43 2.48 2.41 2.48 2.55 2.52 2.46 2.45 2.39 2.4 2.53 2.45 2.33 2.3 2.44 2.39 2.38 2.49 2.46 2.37 2.45 2.29 2.23 2.47 2.4 2.35 2.33 2.4 2.31 1.83 2.36 2.32 2.27 2.24 2.41 2.39 2.28 2.34 2.48 2.52 2.26 2.39 2.38 2.46 2.38 2.23 2.58 2.36 2.35 2.39 2.25 CCa 2.33 2.42 2.27 2.36 2.47 2.36 2.32 2.41 2.31 2.3 2.41 2.35 2.25 2.2 2.34 2.31 2.3 2.39 2.4 2.35 2.35 2.25 2.23 2.35 2.36 2.35 2.35 2.42 2.25 1.95 2.34 2.24 2.15 2.28 2.39 2.35 2.3 2.36 2.44 2.36 2.28 2.31 2.28 2.38 2.28 2.17 2.48 2.32 2.35 2.29 2.25 PO4 1.27 1.05 0.89 1.25 0.84 1.17 1.15 1.06 1.31 1.2 1.32 1.2 0.8 0.83 1.02 1.06 1.19 1.14 1.31 0.85 1.2 0.99 0.88 1.22 1.05 1.07 0.83 1.06 1.1 0.87 1.04 1.02 0.96 0.98 0.87 0.87 1.07 1.07 1.38 1.1 1.05 0.93 0.95 1.16 1.08 1.05 1.12 1.27 0.81 1.16 1.32 Alb 45 43 47 46 44 48 47 42 44 45 46 45 44 45 45 44 44 45 43 41 45 42 40 46 42 40 39 39 43 34 41 44 46 38 41 42 39 39 42 48 39 44 45 44 45 43 45 42 40 45 40 IgA 2.8 2.8 3.5 1.5 1.5 2.4 1.6 2.1 2.6 1.7 2.7 4.3 3.6 3.9 1.6 2.4 1.8 3.3 1.2 1.7 1.3 2.1 2.8 1.9 2.3 1.8 2.5 1.1 3.3 2.2 3.6 2.7 2.6 2.7 3.2 2.9 3.1 1.8 3.1 1.7 2.1 1.2 3.7 1.7 2.4 VitD 35 20 28 47 35 41 19 64 36 38 31 24 65 25 48 31 26 55 73 66 41 39 55 36 28 26 39 57 41 84 50 37 42 40 27 32 43 34 38 42 56 49 37 53 49 57 55 48 68 82 31 tTGA 7.3 6.1 9.4 12.4 7.1 4.6 7.1 4.6 4.6 6.4 3.1 4.6 11.1 10.5 3.8 3.7 6.8 3.4 2.8 4.1 48.8 4.4 3.9 3.1 3.7 3.6 3.3 4.7 3.4 5.3 5.3 3.7 5.3 4.9 4.7 5.5 7.8 6.3 6.8 4.3 8.2 4.5 4.2 8.6 6.6 6.2 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control 25.9 15.8 28.4 20.5 27.9 19.9 21.3 44.2 54 20.2 20.1 21.6 15.3 23.1 10.2 54.4 34.2 9.9 9.9 19.7 7.8 11.5 22 23.2 39 27.2 11.9 20.7 20.3 39.4 24.9 24.9 18.1 27.9 6.6 8.8 12.8 15.2 17.1 21.2 20.3 24.3 13.3 16 28.1 18.9 16.4 32.1 28.3 27.7 11.4 30.7 15 34.4 14.2 12.6 18.8 16.8 25.9 14.6 10.8 4.7 11.8 14.8 5.5 18.6 18.6 13.3 25 22.5 16.9 22.5 22.1 17.6 18.7 13.9 26.6 13.1 17.2 14.6 13.5 17.7 12.1 10.3 17.6 6.8 14.7 10.1 29.4 21.6 15.6 22 13.2 23 18.5 12.1 13.3 20.6 11.3 7.7 16.1 17.8 11.1 10.8 14 14 12.5 22.4 138 140 137 137 136 138 138 136 134 137 135 232 140 140 136 138 140 137 139 137 141 136 137 137 138 138 139 141 140 138 140 138 138 141 139 138 136 138 143 138 139 138 140 137 135 138 138 144 139 139 138 138 142 138 3.7 3.7 4.3 3.5 3.9 3.9 4.1 3.9 3.7 3.7 4.1 3.9 4.2 4.2 4.4 4.3 3.8 3.7 4.1 4.1 4.2 3.9 4.4 4.5 3.6 3.5 3.7 3.5 3.5 3.3 3.9 3.8 3.8 3.6 4.6 4.1 3.8 4.5 3.8 3.6 3.7 3.6 3.9 4.4 4.2 4.1 3.9 3.4 3.7 103 105 101 104 104 105 103 104 101 103 101 106 108 104 102 102 106 103 105 104 107 105 104 102 105 103 102 105 104 101 102 100 101 105 103 104 105 103 107 103 103 103 101 101 101 105 101 108 104 104 102 102 99 103 60 60 70 80 70 60 70 70 60 80 70 70 60 100 100 100 80 100 90 90 110 80 100 80 70 60 60 90 60 90 60 80 80 70 80 100 80 70 110 90 90 100 90 100 90 90 60 70 80 70 80 80 80 70 4.6 6.5 3.6 4.1 4.1 3.1 4.5 4.1 4.6 4.6 4.3 3.4 8.6 5.4 6.3 5.8 5.4 6.8 5.4 4.3 3.7 6.6 4.1 3.9 4.7 4.9 7.5 6.1 7.2 3.8 5.6 5.5 7.5 3.4 3.9 4.7 5.8 4.9 5.1 5.4 6.1 5.6 6.5 5.1 5.6 5.2 6.5 4.8 5.5 6.5 9.6 4.6 lx 2.29 2.37 2.4 2.26 2.37 2.44 2.41 2.29 2.51 2.33 2.43 2.35 2.35 2.28 2.32 2.44 2.27 2.36 2.38 2.41 2.4 2.27 2.36 2.39 2.32 2.5 2.6 2.46 2.39 2.55 2.4 2.53 2.4 2.4 2.31 2.29 2.43 2.37 2.31 2.53 2.37 2.51 2.55 2.46 2.34 2.25 2.51 2.33 2.36 2.28 2.55 2.47 2.69 2.45 2.33 2.35 2.4 2.24 2.35 2.36 2.27 2.33 2.45 2.29 2.35 2.31 2.29 2.34 2.34 2.38 2.29 2.32 2.3 2.25 2.32 2.25 2.36 2.35 2.36 2.56 2.6 2.36 2.35 2.59 2.34 2.45 2.36 2.4 2.27 2.29 2.39 2.29 2.37 2.45 2.37 2.39 2.43 2.4 2.3 2.29 2.37 2.37 2.36 2.34 2.55 2.43 2.57 2.39 1.22 1.11 1.09 1.08 1.85 1.34 1.13 0.92 1.05 1.18 1.3 1.01 1.1 0.97 0.88 0.98 1.09 1.18 1.12 0.95 1.27 1.03 0.97 1.05 1.01 1.15 1.42 1.01 0.9 0.93 1.14 0.87 0.92 1.21 0.83 0.69 0.93 0.99 1.27 1.1 1.02 1.08 1.36 0.95 1.32 1.15 1.34 1.12 1.09 0.82 0.92 1.31 1.6 1.27 38 41 40 41 41 44 47 38 43 42 44 42 43 37 39 43 39 42 44 48 44 41 40 42 38 37 40 45 42 38 43 44 42 40 42 40 42 44 37 44 40 46 46 43 42 38 47 38 40 37 40 42 46 43 3.5 2.2 2.7 1.8 3.3 2.5 1.8 3.1 2.2 1.7 1.7 2.9 1.5 5.6 2.7 2.2 1.8 2.3 2.2 2.3 4.5 3.8 3.9 2.1 2.9 3.5 2.9 3.5 3.5 2.7 3.2 2.1 3.6 1.7 1.3 1.6 3.5 3.5 2.8 3.3 1.1 2.8 1.5 3.7 3.4 1.5 1.8 18 83 45 62 66 59 60 64 75 86 46 45 33 35 47 56 64 56 43 31 70 40 34 49 78 52 53 51 55 58 44 56 57 36 54 37 45 36 44 67 53 46 48 42 41 57 58 92 110 93 86 55 63 98 5.7 3.7 6.5 6.7 5.2 4.4 4.5 7.8 7.8 4.7 5.7 4.1 5.4 5.3 3.9 8.3 7.9 13.2 15.2 6.4 6.7 5.4 4.2 7.8 5.1 4.3 6.7 5.7 5.3 19.9 6.4 5.2 10.6 3.6 4.5 4.9 4.1 26.2 9.8 11.1 4.3 7.7 4.7 4.8 17 4.4 6.5 3.7 3.9 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 Control Control Control Control Control Control Control Control Control Control Control Control Control Control Control Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient 34.5 17.8 33.6 10.2 21.7 31.9 16.2 15.4 22.7 27.2 18.8 13.8 32.9 20.2 20 21.7 12.9 14.6 44.5 18.9 33 31.2 27.2 16.1 18.8 16.6 13.8 34.4 27.7 18.4 18.3 31.6 30.4 33.4 23.6 27.4 15.2 35.8 42.7 28.9 37.4 15.2 25.2 32.8 21.4 41.4 22.4 27.7 30.6 23.3 23 25.2 53 31.5 18 20.2 23.8 11.1 6.7 15.7 11.8 14.4 9.5 12.6 19.2 7.5 14.7 24.9 15 27.7 9.6 30.8 16.8 7.1 17.1 21.4 20.7 10.5 18.8 17 14.1 23.6 27.1 17.1 13.4 10.6 6.2 13.3 11.9 14.4 8.6 30.6 18.9 10.9 12.5 14 17.4 10.5 21 24.1 12.7 15.4 13.9 11.8 26.8 16.3 19.5 20.2 137 140 140 137 138 135 138 138 135 137 139 138 140 140 136 138 138 138 139 138 137 136 138 138 136 136 137 139 135 136 139 136 136 139 137 136 140 136 135 136 139 137 141 137 139 135 137 137 138 137 138 139 138 136 3.5 4.3 4.8 3.6 4 4.2 3.6 4.3 4.4 3.9 4.6 4.1 3.9 3.7 4 3.5 3.7 3.9 4.1 3.8 4.3 3.7 4.3 3.3 3.5 4 3.9 3.9 4.2 3.7 4.2 4.2 3.8 4.1 3.4 4.6 4.5 4.5 4.5 4.1 4.1 4.3 4.5 3.5 4.5 104 105 106 103 102 96 98 99 99 101 102 105 105 104 103 105 104 104 102 104 102 106 107 106 102 103 106 103 105 103 106 103 104 103 105 102 107 103 104 103 104 102 107 106 103 102 102 102 106 103 103 106 105 101 80 100 100 100 80 70 80 70 80 100 60 120 90 80 130 90 60 60 100 70 60 50 60 60 50 60 80 60 60 70 70 60 60 70 70 60 90 60 60 70 60 60 80 60 70 70 70 60 80 60 60 70 80 60 3.9 6.1 5.4 3.4 5.1 3.8 4.1 10.7 5.1 9.9 3.5 6.3 5.6 4.1 4.2 5.1 3.3 3.4 3.5 3.6 5.4 5.5 3.9 5.8 3.3 3.5 2.8 5.9 3.2 3.7 5.9 3.2 4.4 6.3 6.3 5.7 3.5 4.4 4.3 5.8 3.8 4.9 5.2 3.1 5.9 4.4 4.5 lxi 2.31 2.44 2.52 2.4 2.3 2.34 2.63 2.5 2.49 2.42 2.53 2.44 2.41 2.36 2.37 2.44 2.43 2.41 2.46 2.44 2.14 2.16 2.3 2.48 2.39 2.28 2.37 2.54 2.38 2.37 2.28 2.36 2.37 2.51 2.29 2.57 2.32 2.3 2.33 2.38 2.41 2.2 2.42 2.38 2.43 2.42 2.24 2.33 2.27 2.41 2.44 2.38 2.24 2.45 2.37 2.4 2.46 2.36 2.34 2.34 2.57 2.5 2.55 2.46 2.47 2.42 2.37 2.34 2.33 2.38 2.31 2.33 2.34 2.4 2.04 2.1 2.2 2.36 2.37 2.26 2.27 2.48 2.3 2.31 2.22 2.28 2.23 2.39 2.23 2.49 2.22 2.18 2.35 2.32 2.39 2.2 2.32 2.32 2.37 2.4 2.24 2.29 2.19 2.39 2.36 2.34 2.2 2.43 1.01 1.13 1.06 1.1 1.16 1.13 0.98 1.42 1.09 1.25 0.99 1.18 1.03 1.15 1.06 1.33 1.21 0.83 1.35 1.32 0.97 1.09 1.35 1.19 1.15 1.16 1.29 0.71 0.89 1.11 0.99 1.24 0.9 1.41 1.08 1.13 1.41 1.09 1.09 1.3 1.04 1.26 1.19 1.49 1.04 1.17 0.97 1.15 1.3 0.91 1.27 37 42 43 42 38 40 43 40 37 38 43 41 42 41 42 43 46 44 46 42 45 43 45 46 41 41 45 43 44 43 43 44 47 46 43 44 45 46 39 43 41 40 45 43 43 41 40 42 44 41 44 42 42 41 2.5 1.6 1.8 3.2 2.5 2.5 4.1 2.8 2.7 3.1 1.8 3.2 2.8 1.9 3.3 2 1.3 3.1 3.3 1.4 2.3 1.7 3.4 2.8 2.4 2 1.3 2.6 2.4 2.6 2.4 2.1 3.2 2.4 2.1 4.8 2.2 3.7 3.2 2.5 2.8 1.2 2.4 1.9 2.4 2.5 3.1 49 61 67 44 78 48 43 42 98 35 32 70 57 43 60 24 25 26 29 39 98 70 44 45 47 30 55 50 62 27 51 34 36 74 23 29 41 53 54 66 47 69 67 37 39 130 69 55 43 38 41 73 21 54 6.3 5.9 2.9 5.1 11.2 3.7 14.1 5.5 5.9 6.6 5.6 3.7 8.2 5.9 4.8 4.4 7.1 4.1 5.5 3.4 4.6 5.9 4.4 10 4.8 3.8 4.9 7.8 3.5 3.3 4.9 5.9 4.7 3.8 6.8 7.6 6.3 3.7 5.5 12.3 4.8 4.4 5.5 4.4 5.5 3.5 4.3 4.8 7.5 11.4 8.8 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient Patient 40 28.5 10.9 24.2 54.3 33.6 20.2 20 37.7 45.9 40.8 49.4 42.9 24 23.2 54 54 22.3 11.4 16.9 44.9 19.6 23 21.4 27.9 20.2 18.8 13.4 16.5 10.9 14.2 18.3 15.9 18.5 11.6 19.7 11.2 14.9 19.2 16.1 18 5.2 137 138 137 138 138 138 140 140 24 140 139 141 140 138 136 138 139 137 138 138 139 3.8 3.8 3.9 4.6 3.8 3.6 3.7 4.4 4.1 4.1 4.6 4.7 4.1 3.9 3.7 4.4 4.1 3.5 104 107 103 102 106 103 103 104 106 104 102 103 107 104 101 103 105 102 100 102 105 70 60 100 80 70 80 80 60 70 80 80 70 80 60 80 80 70 70 100 110 80 4.4 4.2 4.9 4.2 4.6 4.9 6.4 4.7 5.1 4.6 6.1 5.6 4.3 6.6 7.4 3.1 4.2 6.9 5.7 lxii 2.28 2.16 2.47 2.41 2.55 2.49 2.51 2.41 2.05 2.43 2.46 2.54 2.26 2.33 2.4 2.34 2.51 2.48 2.53 2.34 2.26 2.28 2.18 2.35 2.33 2.53 2.47 2.43 2.29 2.03 2.37 2.46 2.4 2.24 2.29 2.38 2.34 2.49 2.4 2.43 2.38 2.34 1.3 1.03 1.34 0.9 1.21 1.13 1.25 1.2 1.11 1.07 1.26 1.17 0.96 1.26 1.16 0.58 0.92 1.31 1.03 1.35 0.99 40 39 46 44 41 41 44 46 41 43 40 47 41 42 41 40 41 44 45 38 36 1.5 3.6 2.6 6.7 3.1 4.6 1.6 4.5 4.6 3.2 3.4 2.4 1.6 4.6 2.3 2.9 2.2 29 33 68 59 90 74 110 33 25 70 76 54 69 62 42 84 83 76 45 65 82 2.8 8.7 11.8 8.1 5.9 6.9 11.8 3.8 7.2 7.8 10.5 4.8 3.5 37.5 3.5 7.4 3.8 4.3 5.3 Minerva Access is the Institutional Repository of The University of Melbourne Author/s: Truong, Khoa Dang Title: A pilot study of the prevalence of coeliac disease in Vietnamese patients with Graves' disease Date: 2008 Citation: Truong, K D (2008) A pilot study of the prevalence of coeliac disease in Vietnamese patients with Graves' disease Honours thesis, Faculty of Medicine, Dentistry & Health Sciences, Medicine - Royal Melbourne and Western Hospitals, The University of Melbourne Publication Status: Unpublished Persistent Link: http://hdl.handle.net/11343/39573 File Description: A pilot study of the prevalence of coeliac disease in Vietnamese patients with Graves' disease Terms and Conditions: Terms and Conditions: Copyright in works deposited in Minerva Access is retained by the copyright owner The work may not be altered without permission from the copyright owner Readers may only download, print and save electronic copies of whole works for their own personal non-commercial use Any use that exceeds these limits requires permission from the copyright owner Attribution is essential when quoting or paraphrasing from these works