CAN THO UNIVERSITY COLLEGE OF AQUACULTURE AND FISHERIES COMPARING DIFFERENT SUPPLEMENTAL FOODS FOR ARTEMIA IN THE INTENSIVE CULTURE SYSTEM By BUI THANH DIEU A thesis submitted in partial fulfillment of the requirements for the degree of Bachelor of science in Aquaculture Can Tho City, January, 18th 2013 CAN THO UNIVERSITY COLLEGE OF AQUACULTURE AND FISHERIES COMPARING DIFFERENT SUPPLEMENTAL FOODS FOR ARTEMIA IN THE INTENSIVE CULTURE SYSTEM By BUI THANH DIEU A thesis submitted in partial fulfillment of the requirements for the degree of Bachelor of science in Aquaculture Supervisor Assoc.Prof NGUYEN VAN HOA Msc TRAN HUU LE Can Tho City, January, 18th 2013 ACKNOWLEDGEMENT I would like to thank the head of the Department of Aquaculture and Fisheries of Can Tho University has created favorable conditions for learning, studying and teachers imparted to me knowledge that will become the foundation for my future I wish to express my deep appreciation and sincere gratitude to my advisor Dr Pham Minh Duc for his constant guidance to me throughout the time at Can Tho University I sincerely thank Assoc Prof Nguyen Van Hoa and Msc Tran Huu Le have taught and instructed me to complete this thesis I would like to thank Mr Le Van Thong, and his workers in Artemia Experimental farm Vinh Chau - Can Tho University has dedicated help and support during this thesis achievement I would like to thank my classmate who encourage, support and create favorable conditions for me during the study and implementation of the thesis i ABSTRACT The research was conducted in field conditions at Vinh Chau district, Soc Trang province The use of fermented rice bran and tiger shrimp feed (early PL stage) in Artemia intensive production as supplemented food source was evaluated This trial was carried out in earthen pond (500m2 each) with treatments as following: Treatment I (the control known as traditional food supply in Artemia production): Algae+chicken manure; Treatment II: Algae+chicken manure+rice bran and Treatment III: Algae+chicken manure+shrimp feed) Artemia was inoculated at a density of 100 inds/L and culturing for weeks Results indicated that growth and densities of population during the culture were not statistically significant difference among treatments (P>0.05) Similarity, fecundity also did not show statistical difference among them even though the observed fecundity in treatment III was highest (53±18 embryo/female), following by treatment I (43±10 embryo/female) and treatment II was the lowest (42±9 embryo/female) The cyst yield reached 157.22±15.02 kg/ha/crop, was highest in treatment III and significantly differences compared to other treatment (P 0.05) However, the profit rate of treatments T2 was not significant difference compared with the profit rate of treatments T3 (Table 4.1), this is also the direction of open source for poor households not have enough capital (cost of T2 about 58% compared to T3) However, its weakness is the use of rice bran required to pick good food source and farmers need technical experience in the use rice bran as it will be caused pollution and turbidity if it had used too much and long 27 Chapter V CONCLUSIONS AND RECOMMENDATIONS 5.1 Conclusions After weeks of the culture, fecundity of Artemia in treatments fed with tiger shrimp feed (T3) (53±18 embryos/female) is higher than treatments fed fermented rice brain (T2) (42±9 embryos/female) and control treatments (T1) (43±10 embryos/female) but this difference is not statistically significant (p> 0.05) Cysts production in treatments fed with tiger shrimp feed (T3) is highest (157.22±15.02 kg /ha/crop) and statistically significant difference (p0.05) T2 T3 234±13.4a 237±14.6a 275 288 212.5 200 MAX MIN Appendice : yield of cysts in this experiment (g/day) Day 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 TC T1 20 30 10 30 30 20 30 30 30 30 30 40 50 40 30 50 50 20 40 30 40 50 50 40 30 40 50 40 30 T1 30 20 30 30 20 50 50 50 40 30 40 50 40 30 50 50 20 40 30 40 50 40 30 50 70 50 40 30 30 20 A 25 25 20 30 25 35 40 40 35 30 35 45 45 35 40 50 35 30 35 35 45 45 40 45 50 45 45 35 30 20 T2 20 50 60 50 40 30 40 50 40 30 50 50 15 40 30 40 50 40 30 50 70 50 40 30 30 30 40 50 50 80 1010 1150 1090 1275 T2 15 40 30 40 50 40 30 50 70 50 40 30 30 30 40 50 50 80 90 70 90 70 90 40 70 60 60 70 90 70 50 1685 T2 20 50 30 50 70 40 30 50 70 50 40 30 20 30 40 50 50 80 90 70 90 70 90 40 70 60 60 80 90 70 90 1770 A 18 47 40 47 53 37 33 50 60 43 43 37 22 33 37 47 50 67 70 63 83 63 73 37 57 50 53 67 77 73 70 1600 31 T3 40 30 150 70 50 40 30 20 30 40 50 50 100 90 70 90 70 90 100 90 100 120 80 90 100 90 100 90 100 120 T3 10 20 50 100 90 70 90 70 90 100 90 100 120 80 90 100 90 100 90 100 120 90 110 90 110 120 100 100 90 130 T3 36 30 130 90 90 90 70 90 100 90 100 120 70 90 100 90 100 90 100 120 70 110 90 110 120 100 130 90 120 A 2290 2710 2736 2620 29 27 110 87 77 67 63 60 73 77 80 90 97 87 87 93 87 93 97 103 97 107 93 97 110 103 110 93 103 125 Appendice : Cost of experiment a/ T1 (traditional) No content worker (6 x 1) machine Diesel fuel Viscous fuel Pond preparation Artemia Chicken manure Inorganic fertilizer facilities total units month x ind amount price money rate % cost Mean+ STDEV 2,000,000 5,000,000 12,000,000 5,000,000 40.1 16.7 29,900,000 liter liter 100 10 20,000 40,000 2,000,000 400,000 6.7 1.3 29,740,000 can bag 100 500,000 20,000 1,000,000 3,000,000 2,000,000 3.3 10.0 6.7 bag 700,000 3,500,000 1,000,000 29,900,000 11.7 3.3 100.0 mean 29,820,000 stdev 113,137 b/ T2 (fermented rice bran) No content Worker (6 x 1) machine Diesel fuel Viscous fuel Pon preparation Artemia Chicken manure Inorganic fertilizer Fermented rice bran fertilizer total 10 units month x ind amount price money rate % cost Mean+ STDEV 30,098,000 30,086,000 30,080,000 liter liter 100 10 2,000,000 5,000,000 20,000 40,000 12,000,000 5,000,000 2,000,000 400,000 19.6 8.2 3.3 0.7 can bag 100 500,000 20,000 1,000,000 3,000,000 2,000,000 1.6 4.9 3.3 bag 700,000 3,500,000 5.7 kg 33 6,000 198,000 1,000,000 30,098,000 0.3 1.6 49.1 mean 30,088,000 stdev 9,165 32 c/ T3 (shrimp feed No.0) No 10 content Công nhân (6 x ) machine Diesel fuel Viscous Pond preparation Artemia Chicken manure Inorganic fertilizer Shrimp feed No.0 facilities total units month x ind liter liter amount 100 10 money rate % cost Mean+ STDEV 2,000,000 5,000,000 20,000 40,000 12,000,000 5,000,000 2,000,000 400,000 20.8 8.7 3.5 0.7 42,140,000 42,310,000 42,242,000 1.7 5.2 3.5 6.1 price can bag 100 500,000 20,000 1,000,000 3,000,000 2,000,000 bag 700,000 3,500,000 360 34,000 12,240,000 1,000,000 42,140,000 kg 21.2 1.7 73.0 mean 42,230,667 stdev 85,565 33 [...]... stocking Stocking density was 100 nauplii/L we were usually Stocking Instar I stage in the early morning or late evening, avoiding the direction of the wind because they can stick into the pond by waves and wind The water column was 10 cm 3.2.5 Experimental set up Experiment: To determine the effect of fermented rice bran and tiger shrimp feed in Artemia intensive ponds (pond water level maintained...Therefore, the current situation of Artemia cysts production showed that the study to find out a model of high-cyst yield farming (intensive system) , more sustainable and consistent with local conditions is essential 1.2 Objectives of the study The main objective of this study is to evaluate different supplementary foods in the intensive pond system of Artemia culture 1.3 Contents of the study... ranged for development is 7.5 to 8 Artemia distributed in tropical, subtropical and temperate along the coast and inland In Vietnam, an adopted Artemia Franciscana, which originated from the SFB strain (USA), being adapted almost to the Vinh Chau environmental condition after introduction to the site since late 80’s and called Artemia Vinh Chau (or Vinh Chau strain) , they now can grow well in the range... net, sampling net, brine for preservation of Artemia eggs 3.1.3 Artemia cyst source Vinh Chau Artemia cysts (SFB origin, USA), an inoculum provided by the Aquaculture College and Fisheries, Can Tho University 3.1.4 Brine source Brine source is obtained directly from the sea (about 20-30ppt) through the process of evaporation The pond salinity will increase to the level up to 80-90ppt before stocking 12... raised continously at least 20 cm after a week since inoculation - Density: 100 inds/litter - Inoculation: 6-7am in the morning or 7-8pm in the afternoon 2.3.3 Pond management If the pond appears “water-bloom”, we need to prepare again the pond fertilization by raking 1-2 times/day to minimize the development of lab-lab in the pond and provide the food source for Artemia, do not rake when supplying green... avoid water leaking Area (2000-3000m2/pond), dike (wide 3m, high 0,6m) Intensive ponds should be installed with aeration system to supply oxygen in the bottom of the pond, to install a “wave-brake” system at the end of the wind direction to protect the dike and facilitate for cyst harvesting Fertilizer pond The area accounts for 20-25% of the total farming area, water depth ≥ 60 cm If farming area of 2... m2); moreover, combination of complementary foods is one of the manure to increase the cysts yield Feeding and aeration Before feeding, the feed is mixed with water, and then thrown into the pond surface where Artemia being accumulated, try to avoid feeding while supplying water or 10 raking Besides, farmer should use aeration for 6-12h at night when high density of Artemia or standing wind Dose and type... population (Ind/L) : 1 time/week, using sampling net , 200 μm mesh size and surface area (50cm x 50cm), each pond collecting five points (four corners points and one middle point of the pond), all samples obtained into the plastic bucket and used the magnifying glass to count the number of Artemia 15 Growth of Artemia (length in mm): From the first day (at the new stocking) until day 10 (adult Artemia) ,... allocate two fertilized ponds in the green water supply system for rotating 2.3.2 Pond preparation Follow the steps below when the crop started: - Discharge water in the pond - Remove black mud and dry pond 9 - Take seawater and evaporate for salinity increasing (to make saline water) - Salinity ≥ 80 ‰, eliminate predators - Stocking of Artemia Stocking conditions - Salinity: ≥ 80 ‰ - Turbidity: 20-25... geographical regions Artemia can survive very well in natural seawater, but because the body does not have ability to against predators, they can not grow in the sea where there is so much competition and preys Therefore Artemia distributed mainly in waters with high salinity (70 ‰) which limits the predators They can live in high salinities near saturation (250 ‰) Artemia live in an environment with