CAN THO UNIVERSITY COLLEGE OF AQUACULTURE AND FISHERIES Le Hoang Phuong A thesis submitted in partial fulfillment of the requirements for the degree of Bachelor of Aquaculture THE EFFECTS OF PROBIOTICS ON QUALITY POSTLAVAE OF WHITE LEG SHRIMP (Litopenaeus vannamei) 2013 CAN THO UNIVERSITY COLLEGE OF AQUACULTURE AND FISHERIES Le Hoang Phuong A thesis submitted in partial fulfillment of the requirements for the degree of Bachelor of Aquaculture THE EFFECTS OF PROBIOTICS ON QUALITY POSTLAVAE OF WHITE LEG SHRIMP (Litopenaeus vannamei) Supervisor Dr. PHAM MINH DUC Dr. CHAU TAI TAO 2013 ACKNOWLEDGEMENTS First of all, I want to give my honest thank to Rectorate Board of Can Tho University, lectures and instructors of Course of Aquaculture and Fisheries and Auburn University who have facilitated during my studying process in Can Tho city. Secondly, I also want to give my deep gratitude to my supervisor, Dr. Pham Minh Duc and Dr. Chau Tai Tao for valuable guidance, advice, and encouragement. Finally, I would like to give many thank to all my friends in crustacean hatchery, my classmate in advanced aquaculture class that help and encourage me when I thesis. The author, Le Hoang Phuong i ABSTRACT This study aim to evaluate the effects of different types of commercial probiotics (Zimovac, DeoCare, Ecomarine) supplemented in water on growth, survival rate, and quality of white leg shrimp larvae. A triplicated experiment was conducted with different treatments of probiotics including the control (without probiotics). The experiment was conducted in 100-L tanks holding 15,000 larvae (150 larvae/L), and supplied aeration continuously. Brackish water of 30 ppt was used for the experiment. Beginning at Nauplius3 (N3) stage, after larvae transformed to Zoea1 (Z1), the probiotics was added to treatment and re-added every days. During rearing process, the survival rate and length were evaluated at Z3, M3 and PL12 of development stage, beside that time of metamorphosis was investigated when larvae transform to M1 and PL1 of development stage. At the end of experiment, formalin shock and salinity shock was conducted to test the quality of PL12. At zoe3 stage, there was no significant different of survival rate among control treatment and treatments used probiotics, but there was significant different (p[...]... and yield of shrimp whit leg shrimp predominant in the central and southern Central is the main breeding areas of white leg shrimp, accounting for 75.40% of total production of white leg shrimp and 63.30% of the total farming area Meanwhile, the South accounted for only 17.4% of the total production and 19.00% of the total area 6 of farming The rest is the North with 7.20% of the total production and... To find out the appropriate probiotics supply to improve production efficiency and quality of Postlavae of white leg shrimp (Litopenaeus vannamei) 1.3 Research content The effects of using difference probiotics on growth rate, survival rate, and quality of Postlavae 2 CHAPTER 2 LITERATURE REVIEW 2.1 Biological characteristics of white leg shrimp (Litopenaeus vannamei) 2.1.1 Classification: Phylum:... Makridis et al.,(2012) demonstrated that the provision of two strains of bacteria via food directly into the farming water of the incubators of turbot larvae (Scophthalmus maximus) promoted the maintenance of the bacteria in the environment, as well as the colonization of the digestive tract of the larvae Changes in salinity, temperature and dissolved oxygen variations, change the conditions that are favorable... density, which allowed the calculation of the survival of shrimp in the culture tanks, at the same time, 30 animals in each tank were randomly collected to determine the total length Total 13 length of animals were measured from the tip of the rostum to the tip of the telson by millimeter paper under microscope  The quality of the postlarvae were assessed by studying the resistance of the postlarvae to... different organisms, with consequent changes in dominant species, which could lead to the loss of effectiveness of the product Accordingly, the addition of a given probiotic in the farming water of aquatic organisms must be constant, because the conditions of environment suffer periodic changes Thus, the variety of microorganisms present must therefore be considered in the choice of probiotic to be used... 1999) The benefits observed in the supplementation of probiotics in aquaculture include (Verschuere et al.,2000; S Ziaei-Nejad et al.,2004) 1 Improvement of the nutritional value of food; 2 Enzymatic contribution to digestion; 3 Inhibition of pathogens; 4 Growth promoting factors; 5 Improvement in immune response; and 6 Farming water quality Among the most recent studies that point to the effect of the. .. 18.00% of the total area of farming Currently white leg shrimp have been adopted widely in the shrimp farming areas in the country and has effectively economic However, with the widespread adoption today, the risk of environmental pollution, spread of disease causing damage to farmers is unavoidable Therefore, organizations need to plan the breeding areas and invest to research and produce highly quality. .. Although white leg shrimp was cultured around 2000, but its output is still small, only 84,320 tonnes compared with 236,492 tonnes of shrimp in 2009 (MARD, 2009) Till 2010, white leg shrimp farming has spread across North, Central, and South In particular, all of its output is derived from industrial farming Compared with tiger shrimp, white leg shrimp yields only about one third of total production and... external environment and food on the microbial community of this fish Vibrio spp., Plesiomonas shigelloides, and Aeromonas spp are the main causative agents of diseases in aquaculture, and may even cause food infections in humans The interaction between the environment and the host in an aquatic environment is complex The microorganisms present in the water influence the microbiota of the host's intestine... densities, among other stressors (e.g management), which often end up resulting in low growth and feed efficiency rates, besides of weakness in the immune system, making these animals susceptible to the presence of opportunistic pathogens present in the environment In this sense, the effect of probiotics on the immune system has led to a large number of researches with beneficial results on the health of aquatic . stage 13 Table 3.2: Diets for Zoe stage 13 Table 3.3: Feed ingredients for Mysis stage 13 Table 3.4: Diets for Mysis stage 13 Table 3.5: Feed ingredients for Postlavae stage 14 Table 3 .6: Diets. Experiment design 10 3.4 Feeding schedule 11 3.5 Collecting data 13 3 .6 The method for data analysis 14 CHAPTER 4 RESULTS AND DISCUSSIONS 16 iv 4.1 Water quality parameters 17 4.2 White leg. times more than the 264 ,000 tonnes imported in 2000. The United States of America was traditionally supplied with small frozen or processed headless shrimp from Latin 6 America. More recently,