[...]... tissues Multiple transfusions of 2,3-DPG– depleted stored blood can shift the oxygen dissociation curve to the left.11 100 Oxyhemoglobin (% saturation) RBC Preservation Basic Concepts The goal of blood preservation is to provide viable and functional blood components for patients requiring blood transfusion RBC viability is a measure of in vivo RBC survival following transfusion Because blood must be... National Blood Collection and Utilization Survey Report, about 17 million units of whole blood and RBCs were donated in 2008 in the United States.3 Approximately 24 million blood components were transfused in 2008.3 With an aging population and advances in medical treatments requiring transfusions, the demand for blood and blood components can be expected to continue to increase.3 The New York Blood Center... donating their time and expertise because they care about the blood bank profession The book’s intention is to foster improved patient care by providing the reader with a basic understanding of modern blood banking and transfusion practices The sixth edition is designed to generate an unquenchable thirst for knowledge in all medical technologists, blood bankers, and practitioners, whose education, knowledge,... preservation of blood However, the function of glucose in RBC metabolism was not understood until the 1930s Therefore, the common practice of using glucose in the preservative solution was delayed World War II stimulated blood preservation research because the demand for blood and plasma increased The pioneer work of Dr Charles Drew during World War II on developing techniques in blood transfusion and blood. .. as a prelude to the basic concepts of genetics, blood group immunology, and molecular biology (including molecular phenotyping) Part II focuses on blood groups and routine blood bank practices and includes the chapters “Detection and Identification of Antibodies” and “Pretransfusion Testing.” It also covers current technologies and automation Part III, Transfusion Practice,” includes a new chapter called... paid and provide nearly all of the blood used for transfusion in the United States Traditionally, the amount of whole blood in a unit has been 450 mL +/–10% of blood (1 pint) More recently, 500 mL +/–10% of blood are being collected This has provided a small increase in the various components Modified plastic collection systems are used when collecting 500 mL of blood, with the volume of anticoagulant-preservative... Testing Blood Groups and Serologic Testing 5 The Antiglobulin Test 101 6 The ABO Blood Group System 119 7 The Rh Blood Group System 475 495 Part V: Quality and Compliance Issues 23 Quality Management 509 149 8 Blood Group Terminology and the Other Blood Groups 172 9 Detection and Identification of Antibodies 216 10 Pretransfusion... Rejuvenation RBC Freezing Basic Concepts RBC freezing is primarily used for autologous units and the storage of rare blood types Autologous transfusion (auto meaning “self”) allows individuals to donate blood for their own use in meeting their needs for blood transfusion (see Chapter 15, Transfusion Therapy”) The procedure for freezing a unit of packed RBCs is not complicated Basically, it involves the... history of transfusion medicine 2 Describe several biological properties of red blood cells (RBC) that can affect post -transfusion survival 3 Identify the metabolic pathways that are essential for normal RBC function and survival 4 Define the hemoglobin-oxygen dissociation curve, including how it is related to the delivery of oxygen to tissues by transfused RBCs 5 Explain how transfusion of stored blood. .. that transfusions from donor to patient by an unassisted physician became practical An unprecedented accomplishment in blood transfusion was achieved in 1914, when Hustin reported the use of sodium citrate as an anticoagulant solution for transfusions Later, in 1915, Lewisohn determined the minimum amount of citrate needed for anticoagulation and demonstrated its nontoxicity in small amounts Transfusions . index. ISBN 97 8-0 -8 03 6-2 68 2-9 –ISBN 0-8 03 6-2 68 2-7 I. Harmening, Denise. II. Modern blood banking and transfusion practices. III. Title: Modern blood banking and transfusion practices. [DNLM: 1. Blood. Comments RBC RBC RBC/NRBC RBC RBC RBC RBC RBC/NRBC RBC RBC RBC RBC RBC RBC RBC RBC RBC RBC RBC RBC RBC RBC occ occ occ occ occ occ occ occ occ occ occ occ no no rarely rarely no no rare rare no no yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes no no no no no no no no no no no some no no no no no no some some rarely ? occ occ occ occ occ occ occ occ occ occ occ occ rarely no rarely rarely no occ rare ra r e no no yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes warm warm warm warm warm warm warm warm warm warm warm warm warm warm warm warm warm warm warm warm warm warm yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes yes Very. [edited by] Denise Harmening.—6th ed. p. ; cm. Modern blood banking and transfusion practices Rev. ed. of: Modern blood banking and transfusion practices / [edited by] Denise M. Harmening. c2005. Includes