B GIÁO D C VÀ ðÀO T O TRƯ NG ð I H C NÔNG NGHI P HÀ N I NGUY N XUÂN HƯƠNG NGHIÊN C U NHÂN GI NG VÔ TÍNH CÂY HÚNG CHANH Nð (COLEUS FORSKOHLII) B NG K THU T NUÔI C Y MÔ VÀ PHƯƠNG PHÁP GIÂM NG N TRONG H TH NG KHÍ CANH LU N VĂN TH C SĨ HÀ N I – 2013 B GIÁO D C VÀ ðÀO T O TRƯ NG ð I H C NÔNG NGHI P HÀ N I NGUY N XN HƯƠNG NGHIÊN C U NHÂN GI NG VƠ TÍNH CÂY HÚNG CHANH Nð (COLEUS FORSKOHLII) B NG K THU T NUÔI C Y MÔ VÀ PHƯƠNG PHÁP GIÂM NG N TRONG H TH NG KHÍ CANH CHUYÊN NGÀNH : CÔNG NGH SINH H C Mà S : 60.42.02.01 NGƯ I HƯ NG D N KHOA H C: GS.TS NGUY N QUANG TH CH HÀ N I – 2013 L I CAM ðOAN Tơi xin cam đoan s li u k t qu nghiên c u lu n n trung th c chưa ñư c s d ng b t kì m t h c v Tơi xin cam đoan m i s giúp ñ cho vi c th c hi n lu n án ñã ñư c c m ơn thơng tin trích d n lu n n ñ u ñư c ch rõ ngu n g c Tác gi Nguy n Xuân Hương Trư ng ð i h c Nông nghi p Hà N i – Lu n văn th c s khoa h c Nông nghi p ……………………… ii L I C M ƠN ð hoàn thành lu n văn t t nghi p này, s c g ng c a b n thân, tơi nh n đư c r t nhi u s quan tâm, giúp ñ t o m i ñi u ki n thu n l i t th y cơ, gia đình, b n bè đ ng nghi p su t trình h c t p nghiên c u Trư c tiên, xin bày t lòng bi t ơn sâu s c t i th y giáo GS.TS Nguy n Quang Th ch, ngư i t n tình dìu d t, d y b o t o m i ñi u ki n t t nh t đ tơi th c hi n đ tài hồn thành lu n văn t t nghi p Tôi xin c m ơn s nhi t tình ch b o, giúp đ c a Ban lãnh ñ o anh ch Vi n Sinh h c Nông nghi p – Trư ng ð i h c Nông nghi p Hà N i dành cho tơi su t th i gian th c hi n đ tài Tơi xin g i l i bi t ơn sâu s c đ n gia đình tồn th b n bè ñã ñ ng viên giúp ñ su t trình h c t p trình hồn thành khóa lu n Tác gi Nguy n Xuân Hương Trư ng ð i h c Nông nghi p Hà N i – Lu n văn th c s khoa h c Nông nghi p ……………………… iii M CL C L i cam ñoan ii L i c m ơn iii M cl c iv Danh m c ch vi t t t vi Danh m c b ng vii Danh m c hình ix M ð U Chương T NG QUAN TÀI LI U 1.1 Gi i thi u v húng chanh n ð 1.1.1 Ngu n g c ñ c ñi m th c v t h c b n c a húng chanh n ð 1.1.2 B ph n s d ng thành ph n hóa h c 1.1.3 Tác d ng dư c lý 1.2 T ng quan tình hình nghiên c u húng chanh n ð 11 1.2.1 Nhân gi ng b ng phương pháp truy n th ng 11 1.2.2 Nhân gi ng in vitro 12 1.3 Phương pháp khí canh 13 1.3.1 Khái ni m 13 1.3.2 Ưu, c m c a phương pháp khí canh 14 1.3.3 M t s nhân t k thu t nh hư ng đ n q trình nhân gi ng tr ng tr t b ng phương pháp khí canh 15 Chương V T LI U – N I DUNG - PHƯƠNG PHÁP NGHIÊN C U 20 2.1 ð i tư ng v t li u nghiên c u 20 2.1.1 ð i tư ng nghiên c u 20 2.1.2 V t li u nghiên c u 20 2.2 Th i gian ñ a ñi m nghiên c u 21 2.3 N i dung nghiên c u 21 2.3.1 Nghiên c u nhân gi ng húng chanh n ð b ng k thu t nuôi c y mô 21 2.3.2 Nghiên c u k thu t tr ng húng chanh n ð b ng phương pháp khí canh Trư ng ð i h c Nông nghi p Hà N i – Lu n văn th c s khoa h c Nông nghi p ……………………… 22 iv 2.4 Phương pháp nghiên c u 22 2.4.1 Phương pháp b trí thí nghi m 22 2.4.2 Các ch tiêu theo dõi phương pháp xác ñ nh 26 2.4.3 Phương pháp x lý s li u 27 Chương K T QU VÀ TH O LU N 28 3.1 28 3.1.1 Nghiên c u nhân gi ng húng chanh n ð b ng nuôi c y mơ nh hư ng c a BA Kinetin đ n kh phát sinh ch i húng chanh n ð 28 3.1.2 nh hư ng c a IAA Kinetin ñ n h s nhân ch i húng chanh n ð 32 3.1.3 nh hư ng c a α-NAA ñ n t l r r ch i húng chanh n ð 35 3.1.4 nh hư ng c a mơi trư ng đ n s sinh trư ng, phát tri n c a húng chanh n ð 3.2 Nghiên c u nh hư ng c a m t s y u t ñ n kh sinh trư ng, phát tri n c a húng chanh n ð tr ng b ng phương pháp khí canh 3.2.1 48 nh hư ng c a nhi t ñ dung d ch ñ n kh sinh trư ng, phát tri n c a húng chanh n ð v Hè 3.3 46 nh hư ng kho ng cách gi a hai l n phun ñ n kh sinh trư ng, phát tri n c a húng chanh n ð 3.2.5 43 nh hư ng c a pH dung d ch ñ n kh sinh trư ng, phát tri n c a húng chanh n ð 3.2.4 40 nh hư ng c a ngư ng EC dung d ch dinh dư ng ñ n kh sinh trư ng, phát tri n c a húng chanh n ð 3.2.3 39 nh hư ng c a dung d ch dinh dư ng ñ n kh sinh trư ng, phát tri n c a húng chanh n ð 3.2.2 37 50 nh hư ng c a phương th c tr ng tr t ñ n kh sinh trư ng, phát tri n c a húng chanh n ð 52 K T LU N VÀ ð NGH 56 K t lu n 56 ð ngh 56 TÀI LI U THAM KH O 57 PH L C 61 Trư ng ð i h c Nông nghi p Hà N i – Lu n văn th c s khoa h c Nông nghi p ……………………… v DANH M C CH VI T T T TT Ch vi t t t T vi t t t 2,4 D α-NAA AMP BA cAMP Cyclic adenosine monophosphate cGMP Cyclic guanosine monophosphate CT Công th c ðC ð i ch ng EC (Electrical conductivity)- ñ d n ñi n c a dung d ch 10 IAA Indol 3-acetic acid 11 IBA Indol 3-butyric acid 12 Kinetin 13 MS Murashinge and Skoog, 1962 14 NST Ngày sau tr ng 2.4 dichlorophenoxyacetic acid α-naphlene acetic acid Adenosine monophosphate – benzylaminopurine 6-Furfurylaminopurine Trư ng ð i h c Nông nghi p Hà N i – Lu n văn th c s khoa h c Nông nghi p ……………………… vi DANH M C B NG STT 1.1 3.1 Tên b ng Trang Th n ph n hóa h c phân l p ñư c t húng chanh n ð nh hư ng c a n ng ñ BA ñ n kh phát sinh ch i húng chanh n ð (sau 30 ngày) 3.2 29 nh hư ng c a n ng ñ Kinetin ñ n kh phát sinh ch i húng chanh n ð (sau 30 ngày) 3.3 30 nh hư ng c a t h p n ng ñ BA Kinetin ñ n kh phát sinh ch i húng chanh n ð (sau 30 ngày) 3.4 nh hư ng c a t h p IAA Kinetin ñ n hi u qu 31 nhân ch i húng chanh n ð (sau 30 ngày) 3.5 46 nh hư ng c a pH dung d ch đ n kh tích lũy v t ch t khô c a húng chanh n ð 3.13 45 nh hư ng c a pH dung d ch ñ n kh sinh trư ng, phát tri n c a húng chanh n ð (sau 13 tu n tr ng) 3.12 43 nh hư ng c a n ng ñ dung d ch ñ n kh tích lũy v t ch t khơ c a húng chanh n ð 3.11 42 nh hư ng c a n ng ñ dung d ch dinh dư ng ñ n kh sinh trư ng, phát tri n c a húng chanh n ð (sau 13 tu n tr ng) 3.10 41 nh hư ng c a lo i dung d ch dinh dư ng ñ n kh tích lũy v t ch t khơ c a húng chanh n ð 3.9 38 nh hư ng c a lo i dung d ch dinh dư ng ñ n kh sinh trư ng, phát tri n c a húng chanh n ð (sau 13 tu n tr ng) 3.8 35 nh hư ng c a mơi trư ng đ n s sinh trư ng, phát tri n c a húng chanh n ð (sau 30 ngày) 3.7 33 nh hư ng c a n ng ñ α-NAA ñ n hi u qu r húng chanh n ð (sau 30 ngày) 3.6 47 nh hư ng c a th i gian ngh phun ñ n kh sinh trư ng, phát tri n c a húng chanh n ð (sau 13 tu n tr ng) Trư ng ð i h c Nông nghi p Hà N i – Lu n văn th c s khoa h c Nông nghi p ……………………… 48 vii 3.14 nh hư ng c a th i gian ngh phun ñ n kh tích lũy v t ch t khơ c a húng chanh n ð 3.15 Theo dõi di n bi n nhi t ñ vùng r húng chanh 49 nð cơng th c thí nghi m 3.16 nh hư ng c a nhi t ñ ñ n kh sinh trư ng, phát tri n c a húng chanh n ð (sau 13 tu n tr ng) 3.17 51 nh hư ng c a nhi t ñ dung d ch ñ n kh tích lũy v t ch t khô c a húng chanh n ð 3.18 51 52 nh hư ng c a phương th c tr ng tr t ñ n sinh trư ng, phát tri n c a húng chanh n ð (sau 13 tu n tr ng) 3.19 nh hư ng c a phương th c tr ng tr t đ n di n tích 3.20 53 nh hư ng c a phương th c tr ng tr t đ n kh tích lũy v t ch t khô c a húng chanh n ð Trư ng ð i h c Nông nghi p Hà N i – Lu n văn th c s khoa h c Nông nghi p ……………………… 53 54 viii DANH M C HÌNH STT Tên hình Trang 1.1 Cây húng chanh n ð 1.2 C húng chanh n ð 1.3 H p ch t forskolin 1.4 Ru ng tr ng húng chanh n ð 11 1.5 Sơ ñ h th ng khí canh 14 2.1 Các d ng thi t b khí canh t thi t k c a Vi n Sinh h c Nông nghi p 21 2.2 B khí canh nh có thi t k b ph n làm mát dung d ch 21 3.1 nh hư ng c a n ng ñ BA ñ n t l m u b t ch i 29 3.2 nh hư ng c a n ng ñ kinetin ñ n t l m u b t ch i 30 3.3 nh hư ng c a t h p BA Kinetin ñ n t l m u b t ch i 31 3.4 nh hư ng ph i h p c a n ng ñ IAA Kinetin ñ n h s nhân nhanh ch i húng chanh n ð 33 3.5 Ch i húng chanh n ð phát sinh môi trư ng nhân nhanh 34 3.6 Ch i d d ng 34 3.7 nh hư ng c a n ng ñ α-NAA ñ n t l r húng chanh n ð 3.8 nh hư ng c a n ng ñ α-NAA ñ n s sinh trư ng, phát tri n c a ch i húng chanh n ð 36 37 3.9 nh hư ng c a n ng ñ α-NAA ñ n hi u qu r 37 3.10 nh hư ng c a môi trư ng ñ n t l s ng c a húng chanh n ð 38 3.11 Húng chanh n ð tr ng ñ t 39 3.12 Húng chanh n ð h th ng khí canh 39 3.13 Húng chanh n ð EC=500 S/cm 44 3.14 Húng chanh n ð EC=1000 S/cm 44 3.15 nh hư ng c a phương th c tr ng tr t ñ n di n tích húng chanh n ð 3.16 nh hư ng c a phương th c tr ng tr t đ n kh tích lũy v t ch t khô c a húng chanh n ð Trư ng ð i h c Nông nghi p Hà N i – Lu n văn th c s khoa h c Nông nghi p ……………………… 54 55 ix * RESIDUAL 4.52280 753800 * TOTAL (CORRECTED) 49.3904 6.17380 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE CKHOMT 13/ 9/** 18:34 PAGE ANH HUONG CUA DUNG DICH DINH DUONG DEN KHA NANG TICH LUY VAT CHAT KHO MEANS FOR EFFECT CT$ CT$ MT KT1 MT KT2 MT HOAGLAN NOS 3 30NST 6.04667 5.93667 6.17667 60NST 11.2700 11.0833 11.8933 90NST 20.4733 19.2767 24.4967 SE(N= 3) 0.135933 0.131895 0.501265 5%LSD 6DF 0.470214 0.456246 1.73396 ANALYSIS OF VARIANCE SUMMARY TABLE FILE CKHOMT 13/ 9/** 18:34 PAGE ANH HUONG CUA DUNG DICH DINH DUONG DEN KHA NANG TICH LUY VAT CHAT KHO F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE 30NST 60NST 90NST GRAND MEAN (N= 9) NO OBS 6.0533 11.416 21.416 STANDARD DEVIATION C OF V |CT$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 0.22891 0.23544 3.9 0.5022 0.41723 0.22845 2.0 0.0120 2.4847 0.86822 4.1 0.0011 | | | | nh hư ng c a n ng ñ dung d ch BALANCED ANOVA FOR VARIATE CH CAO FILE CCAOEC 19/ 7/** 10: PAGE ANH HUONG CUA NONG DO DUNG DICH DEN CHIEU CAO HUNG CHANH VARIATE V003 CH CAO CAO CAO CAO CAO LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 1823.95 607.982 116.35 0.000 * RESIDUAL 41.8034 5.22542 * TOTAL (CORRECTED) 11 1865.75 169.614 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE CCAOEC 19/ 7/** 10: PAGE ANH HUONG CUA NONG DO DUNG DICH DEN CHIEU CAO HUNG CHANH MEANS FOR EFFECT CT$ CT$ EC=500 EC=1000 EC=1500 EC=2000 NOS 3 3 CH CAO 54.7000 85.5000 83.7133 78.3100 SE(N= 3) 1.31978 5%LSD 8DF 4.30365 ANALYSIS OF VARIANCE SUMMARY TABLE FILE CCAOEC 19/ 7/** 10: PAGE ANH HUONG CUA NONG DO DUNG DICH DEN CHIEU CAO HUNG CHANH F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE GRAND MEAN STANDARD DEVIATION C OF V |CT$ | Trư ng ð i h c Nông nghi p Hà N i – Lu n văn th c s khoa h c Nông nghi p ……………………… 71 CH CAO (N= 12) NO OBS 12 75.556 SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 13.024 2.2859 3.0 0.0000 | | | BALANCED ANOVA FOR VARIATE SO LA FILE SOLAEC 19/ 7/** 10:13 PAGE ANH HUONG CUA NONG DO DUNG DICH DEN SO LA HUNG CHANH VARIATE V003 SO LA LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 10320.3 3440.11 102.95 0.000 * RESIDUAL 267.333 33.4166 * TOTAL (CORRECTED) 11 10587.7 962.515 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE SOLAEC 19/ 7/** 10:13 PAGE ANH HUONG CUA NONG DO DUNG DICH DEN SO LA HUNG CHANH MEANS FOR EFFECT CT$ CT$ EC=500 EC=1000 EC=1500 EC=2000 NOS 3 3 SO LA 154.667 225.000 226.667 193.000 SE(N= 3) 3.33750 5%LSD 8DF 10.8832 ANALYSIS OF VARIANCE SUMMARY TABLE FILE SOLAEC 19/ 7/** 10:13 PAGE ANH HUONG CUA NONG DO DUNG DICH DEN SO LA HUNG CHANH F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE SO LA GRAND MEAN (N= 12) NO OBS 12 199.83 STANDARD DEVIATION C OF V |CT$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 31.024 5.7807 2.9 0.0000 | | | | BALANCED ANOVA FOR VARIATE S LA FILE SLAEC 26/ 8/** 10: PAGE ANH HUONG CUA EC DUNG DICH DEN DIEN TICH LA HUNG CHANH VARIATE V003 S LA LA LA LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 391197E+07 130399E+07 88.17 0.000 * RESIDUAL 118314 14789.3 * TOTAL (CORRECTED) 11 403028E+07 366389 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE SLAEC 26/ 8/** 10: PAGE ANH HUONG CUA EC DUNG DICH DEN DIEN TICH LA HUNG CHANH MEANS FOR EFFECT CT$ CT$ EC=500 EC=1000 EC=1500 EC=2000 NOS 3 3 S LA 1046.97 2472.97 2396.40 2123.63 Trư ng ð i h c Nông nghi p Hà N i – Lu n văn th c s khoa h c Nông nghi p ……………………… 72 SE(N= 3) 70.2122 5%LSD 8DF 228.955 ANALYSIS OF VARIANCE SUMMARY TABLE FILE SLAEC 26/ 8/** 10: PAGE ANH HUONG CUA EC DUNG DICH DEN DIEN TICH LA HUNG CHANH F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE S LA GRAND MEAN (N= 12) NO OBS 12 2010.0 STANDARD DEVIATION C OF V |CT$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 605.30 121.61 6.1 0.0000 | | | | BALANCED ANOVA FOR VARIATE 30NST FILE CKHOEC 13/ 9/** 18: PAGE ANH HUONG CUA EC DUNG DICH DEN KHA NANG TICH LUY CHAT KHO VARIATE V003 30NST LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 1.66462 554875 24.88 0.000 * RESIDUAL 178400 223000E-01 * TOTAL (CORRECTED) 11 1.84303 167548 BALANCED ANOVA FOR VARIATE 60NST FILE CKHOEC 13/ 9/** 18: PAGE ANH HUONG CUA EC DUNG DICH DEN KHA NANG TICH LUY CHAT KHO VARIATE V004 60NST LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 98.2204 32.7401 654.79 0.000 * RESIDUAL 400009 500012E-01 * TOTAL (CORRECTED) 11 98.6204 8.96549 BALANCED ANOVA FOR VARIATE 90NST FILE CKHOEC 13/ 9/** 18: PAGE ANH HUONG CUA EC DUNG DICH DEN KHA NANG TICH LUY CHAT KHO VARIATE V005 90NST LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 273.948 91.3159 287.21 0.000 * RESIDUAL 2.54351 317939 * TOTAL (CORRECTED) 11 276.491 25.1356 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE CKHOEC 13/ 9/** 18: PAGE ANH HUONG CUA EC DUNG DICH DEN KHA NANG TICH LUY CHAT KHO MEANS FOR EFFECT CT$ CT$ EC=500 EC=1000 EC=1500 EC=2000 NOS 3 3 30NST 5.39333 6.43000 6.03333 6.05333 60NST 6.53000 14.0633 12.5033 12.2533 90NST 8.52667 20.9333 18.6900 18.3300 Trư ng ð i h c Nông nghi p Hà N i – Lu n văn th c s khoa h c Nông nghi p ……………………… 73 SE(N= 3) 0.862169E-01 0.129101 0.325545 5%LSD 8DF 0.281144 0.420985 1.06157 ANALYSIS OF VARIANCE SUMMARY TABLE FILE CKHOEC 13/ 9/** 18: PAGE ANH HUONG CUA EC DUNG DICH DEN KHA NANG TICH LUY CHAT KHO F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE 30NST 60NST 90NST GRAND MEAN (N= 12) NO OBS 12 5.9775 12 11.337 12 16.620 STANDARD DEVIATION C OF V |CT$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 0.40933 0.14933 2.5 0.0003 2.9942 0.22361 2.0 0.0000 5.0135 0.56386 3.4 0.0000 | | | | nh hư ng c a pH dung d ch BALANCED ANOVA FOR VARIATE CCAO FILE CCAOPH1 21/ 8/** 10:36 PAGE ANH HUONG CUA pH DUNG DICH DEN CHIEU CAO HUNG CHANH VARIATE V003 CCAO LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 390.777 195.388 11.81 0.009 * RESIDUAL 99.2283 16.5380 * TOTAL (CORRECTED) 490.005 61.2506 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE CCAOPH1 21/ 8/** 10:36 PAGE ANH HUONG CUA pH DUNG DICH DEN CHIEU CAO HUNG CHANH MEANS FOR EFFECT CT$ CT$ pH=5.5 pH=6.5 pH=7.5 NOS 3 CCAO 70.9000 79.0867 87.0400 SE(N= 3) 2.34791 5%LSD 6DF 8.12180 ANALYSIS OF VARIANCE SUMMARY TABLE FILE CCAOPH1 21/ 8/** 10:36 PAGE ANH HUONG CUA pH DUNG DICH DEN CHIEU CAO HUNG CHANH F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE CCAO GRAND MEAN (N= 9) NO OBS 79.009 STANDARD DEVIATION C OF V |CT$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 7.8263 4.0667 5.1 0.0089 | | | | BALANCED ANOVA FOR VARIATE SO LA FILE SO LA PH 21/ 8/** 10:29 PAGE ANH HUONG CUA pH DUNG DICH DEN SO LA HUNG CHANH VARIATE V003 SO LA LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 988.027 494.014 13.14 0.007 * RESIDUAL 225.493 37.5822 - Trư ng ð i h c Nông nghi p Hà N i – Lu n văn th c s khoa h c Nông nghi p ……………………… 74 * TOTAL (CORRECTED) 1213.52 151.690 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE SO LA PH 21/ 8/** 10:29 PAGE ANH HUONG CUA pH DUNG DICH DEN SO LA HUNG CHANH MEANS FOR EFFECT CT$ CT$ pH=5.5 pH=6.5 pH=7.5 NOS 3 SO LA 223.200 248.333 240.267 SE(N= 3) 3.53941 5%LSD 6DF 12.2434 ANALYSIS OF VARIANCE SUMMARY TABLE FILE SO LA PH 21/ 8/** 10:29 PAGE ANH HUONG CUA pH DUNG DICH DEN SO LA HUNG CHANH F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE SO LA GRAND MEAN (N= 9) NO OBS 237.27 STANDARD DEVIATION C OF V |CT$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 12.316 6.1304 2.6 0.0070 | | | | BALANCED ANOVA FOR VARIATE S LA FILE SLAPH 26/ 8/** 10:19 PAGE ANH HUONG CUA pH DUNG DICH DEN DIEN TICH LA HUNG CHANH VARIATE V003 S LA LA LA LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 414653 207326 6.92 0.028 * RESIDUAL 179738 29956.3 * TOTAL (CORRECTED) 594391 74298.8 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE SLAPH 26/ 8/** 10:19 PAGE ANH HUONG CUA pH DUNG DICH DEN DIEN TICH LA HUNG CHANH MEANS FOR EFFECT CT$ CT$ pH=5.5 pH=6.5 pH=7.5 NOS 3 S LA 2259.60 2771.73 2618.70 SE(N= 3) 99.9272 5%LSD 6DF 345.664 ANALYSIS OF VARIANCE SUMMARY TABLE FILE SLAPH 26/ 8/** 10:19 PAGE ANH HUONG CUA pH DUNG DICH DEN DIEN TICH LA HUNG CHANH F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE S LA GRAND MEAN (N= 9) NO OBS 2550.0 STANDARD DEVIATION C OF V |CT$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 272.58 173.08 6.8 0.0281 | | | | Trư ng ð i h c Nông nghi p Hà N i – Lu n văn th c s khoa h c Nông nghi p ……………………… 75 BALANCED ANOVA FOR VARIATE 30NST FILE CKHOPH 13/ 9/** 11: PAGE ANH HUONG CUA pH DUNG DICH DEN KHA NANG TICH LUY VAT CHO KHO VARIATE V003 30NST LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 568986E-01 284493E-01 0.87 0.467 * RESIDUAL 195189 325315E-01 * TOTAL (CORRECTED) 252088 315110E-01 BALANCED ANOVA FOR VARIATE 60NST FILE CKHOPH 13/ 9/** 11: PAGE ANH HUONG CUA pH DUNG DICH DEN KHA NANG TICH LUY VAT CHO KHO VARIATE V004 60NST LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 1.00909 504544 7.37 0.025 * RESIDUAL 410866 684777E-01 * TOTAL (CORRECTED) 1.41995 177494 BALANCED ANOVA FOR VARIATE 90NST FILE CKHOPH 13/ 9/** 11: PAGE ANH HUONG CUA pH DUNG DICH DEN KHA NANG TICH LUY VAT CHO KHO VARIATE V005 90NST LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 29.8281 14.9140 9.55 0.014 * RESIDUAL 9.37434 1.56239 * TOTAL (CORRECTED) 39.2024 4.90030 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE CKHOPH 13/ 9/** 11: PAGE ANH HUONG CUA pH DUNG DICH DEN KHA NANG TICH LUY VAT CHO KHO MEANS FOR EFFECT CT$ CT$ pH=5.5 pH=6.5 pH=7.5 NOS 3 30NST 4.94667 5.10600 5.12333 60NST 10.1600 10.4033 10.9600 90NST 17.6700 22.0667 20.5133 SE(N= 3) 0.104134 0.151082 0.721662 5%LSD 6DF 0.360215 0.522618 2.49635 ANALYSIS OF VARIANCE SUMMARY TABLE FILE CKHOPH 13/ 9/** 11: PAGE ANH HUONG CUA pH DUNG DICH DEN KHA NANG TICH LUY VAT CHO KHO F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE 30NST 60NST 90NST GRAND MEAN (N= 9) NO OBS 5.0587 10.508 20.083 STANDARD DEVIATION C OF V |CT$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 0.17751 0.18037 3.6 0.4665 0.42130 0.26168 2.5 0.0247 2.2137 1.2500 6.2 0.0143 | | | | Trư ng ð i h c Nông nghi p Hà N i – Lu n văn th c s khoa h c Nông nghi p ……………………… 76 nh hư ng c a th i gian ngh phun BALANCED ANOVA FOR VARIATE CHCAO FILE CCAOTG1 7/ 7/** 10:26 PAGE ANH HUONG CUA THOI GIAN NGHI PHUN DEN CHIEU CAO HUNG CHANH VARIATE V003 CHCAO LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 452.776 226.388 94.64 0.000 * RESIDUAL 14.3533 2.39222 * TOTAL (CORRECTED) 467.129 58.3911 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE CCAOTG1 7/ 7/** 10:26 PAGE ANH HUONG CUA THOI GIAN NGHI PHUN DEN CHIEU CAO HUNG CHANH MEANS FOR EFFECT CT$ CT$ 10-15 15-30 30-45 NOS 3 CHCAO 91.9333 78.8333 75.5000 SE(N= 3) 0.892976 5%LSD 6DF 3.08895 ANALYSIS OF VARIANCE SUMMARY TABLE FILE CCAOTG1 7/ 7/** 10:26 PAGE ANH HUONG CUA THOI GIAN NGHI PHUN DEN CHIEU CAO HUNG CHANH F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE GRAND MEAN (N= 9) NO OBS 82.089 CHCAO STANDARD DEVIATION C OF V |CT$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 7.6414 1.5467 1.9 0.0001 | | | | BALANCED ANOVA FOR VARIATE SO LA FILE SOLATG 21/ 8/** 10:43 PAGE ANH HUONG CUA THOI GIAN NGHI PHUN DEN SO LA HUNG CHANH VARIATE V003 SO LA LA LA LA LA LA LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 2918.21 1459.10 18.15 0.003 * RESIDUAL 482.233 80.3722 * TOTAL (CORRECTED) 3400.44 425.055 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE SOLATG 21/ 8/** 10:43 PAGE ANH HUONG CUA THOI GIAN NGHI PHUN DEN SO LA HUNG CHANH MEANS FOR EFFECT CT$ CT$ 10-15 15-30 30-45 SE(N= 5%LSD 3) 6DF NOS 3 SO LA 254.333 235.400 210.367 5.17598 17.9045 Trư ng ð i h c Nông nghi p Hà N i – Lu n văn th c s khoa h c Nông nghi p ……………………… 77 ANALYSIS OF VARIANCE SUMMARY TABLE FILE SOLATG 21/ 8/** 10:43 PAGE ANH HUONG CUA THOI GIAN NGHI PHUN DEN SO LA HUNG CHANH F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE SO LA GRAND MEAN (N= 9) NO OBS 233.37 STANDARD DEVIATION C OF V |CT$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 20.617 8.9651 3.8 0.0034 | | | | BALANCED ANOVA FOR VARIATE S LA FILE SLATG 26/ 8/** 10:31 PAGE ANH HUONG CUA THOI GIAN NGHI PHUN DEN DIEN TICH LA HUNG CHANH VARIATE V003 S LA LA LA LA LA LA LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 983826 491913 30.28 0.001 * RESIDUAL 97472.0 16245.3 * TOTAL (CORRECTED) 108130E+07 135162 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE SLATG 26/ 8/** 10:31 PAGE ANH HUONG CUA THOI GIAN NGHI PHUN DEN DIEN TICH LA HUNG CHANH MEANS FOR EFFECT CT$ CT$ 10-15 15-30 30-45 NOS 3 S LA 2886.67 2481.70 2076.80 SE(N= 3) 73.5874 5%LSD 6DF 254.551 ANALYSIS OF VARIANCE SUMMARY TABLE FILE SLATG 26/ 8/** 10:31 PAGE ANH HUONG CUA THOI GIAN NGHI PHUN DEN DIEN TICH LA HUNG CHANH F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE S LA GRAND MEAN (N= 9) NO OBS 2481.7 STANDARD DEVIATION C OF V |CT$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 367.64 127.46 5.1 0.0010 | | | | BALANCED ANOVA FOR VARIATE 30NST FILE CKHOTG 13/ 9/** 10:39 PAGE ANH HUONG CUA THOI GIAN NGHI PHUN DEN KHA NANG TICH LUY CHAT KHO VARIATE V003 30NST LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 1.67629 838145 66.46 0.000 * RESIDUAL 756667E-01 126111E-01 * TOTAL (CORRECTED) 1.75196 218994 - BALANCED ANOVA FOR VARIATE 60NST FILE CKHOTG 13/ 9/** 10:39 PAGE Trư ng ð i h c Nông nghi p Hà N i – Lu n văn th c s khoa h c Nông nghi p ……………………… 78 ANH HUONG CUA THOI GIAN NGHI PHUN DEN KHA NANG TICH LUY CHAT KHO VARIATE V004 60NST LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 9.00029 4.50014 90.10 0.000 * RESIDUAL 299668 499446E-01 * TOTAL (CORRECTED) 9.29996 1.16249 BALANCED ANOVA FOR VARIATE 90NST FILE CKHOTG 13/ 9/** 10:39 PAGE ANH HUONG CUA THOI GIAN NGHI PHUN DEN KHA NANG TICH LUY CHAT KHO VARIATE V005 90NST LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 53.2061 26.6030 39.07 0.001 * RESIDUAL 4.08513 680855 * TOTAL (CORRECTED) 57.2912 7.16140 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE CKHOTG 13/ 9/** 10:39 PAGE ANH HUONG CUA THOI GIAN NGHI PHUN DEN KHA NANG TICH LUY CHAT KHO MEANS FOR EFFECT CT$ CT$ 10-15 15-30 30-45 NOS 3 30NST 6.71000 5.84667 5.75000 60NST 11.7800 11.0200 9.38333 90NST 23.3933 19.5567 17.5300 SE(N= 3) 0.648360E-01 0.129028 0.476394 5%LSD 6DF 0.224278 0.446328 1.64792 ANALYSIS OF VARIANCE SUMMARY TABLE FILE CKHOTG 13/ 9/** 10:39 PAGE ANH HUONG CUA THOI GIAN NGHI PHUN DEN KHA NANG TICH LUY CHAT KHO F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE 30NST 60NST 90NST GRAND MEAN (N= 9) NO OBS 6.1022 10.728 20.160 STANDARD DEVIATION C OF V |CT$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 0.46797 0.11230 1.8 0.0002 1.0782 0.22348 2.1 0.0001 2.6761 0.82514 4.1 0.0006 | | | | nh hư ng c a nhi t ñ dung d ch BALANCED ANOVA FOR VARIATE CH CAO FILE CHCAONDO 7/ 7/** 11:32 PAGE ANH HUONG CUA NHIET DO DEN CHIEU CAO HUNG CHANH VARIATE V003 CH CAO CAO LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 567.845 567.845 66.03 0.000 * RESIDUAL 51.5950 8.59917 * TOTAL (CORRECTED) 619.440 88.4914 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE CHCAONDO 7/ 7/** 11:32 PAGE Trư ng ð i h c Nông nghi p Hà N i – Lu n văn th c s khoa h c Nông nghi p ……………………… 79 ANH HUONG CUA NHIET DO DEN CHIEU CAO HUNG CHANH MEANS FOR EFFECT CT$ - CT$ NOS 4 CT1 CT2 CHCAO 96.1750 79.3250 SE(N= 4) 1.46622 5%LSD 6DF 5.07188 ANALYSIS OF VARIANCE SUMMARY TABLE FILE CHCAONDO 7/ 7/** 11:32 PAGE ANH HUONG CUA NHIET DO DEN CHIEU CAO HUNG CHANH F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE CHCAO GRAND MEAN (N= 8) NO OBS 87.750 STANDARD DEVIATION C OF V |CT$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 9.4070 2.9324 3.3 0.0003 | | | | BALANCED ANOVA FOR VARIATE SOLA FILE SOLAND1 7/ 7/** 13:14 PAGE ANH HUONG CUA NHIET DO DUNG DICH DEN SO LA HUNG CHANH VARIATE V003 SOLA LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 1035.12 1035.12 28.39 0.002 * RESIDUAL 218.735 36.4559 * TOTAL (CORRECTED) 1253.86 179.123 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE SOLAND1 7/ 7/** 13:14 PAGE ANH HUONG CUA NHIET DO DUNG DICH DEN SO LA HUNG CHANH MEANS FOR EFFECT CT$ CT$ NOS CT1 CT2 4 SE(N= 4) 5%LSD 6DF - SOLA 274.075 251.325 3.01893 10.4430 ANALYSIS OF VARIANCE SUMMARY TABLE FILE SOLAND1 7/ 7/** 13:14 PAGE ANH HUONG CUA NHIET DO DUNG DICH DEN SO LA HUNG CHANH F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE SOLA GRAND MEAN STANDARD DEVIATION C OF V |CT$ | (N= 8) SD/MEAN | | NO BASED ON BASED ON % | | OBS TOTAL SS RESID SS | | 262.70 13.384 6.0379 2.3 0.0021 BALANCED ANOVA FOR VARIATE SLA FILE SLAND 26/ 8/** 10:38 PAGE ANH HUONG CUA NHIET DO DUNG DICH DEN DIEN TICH LA HUNG CHANH VARIATE V003 SLA LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER Trư ng ð i h c Nông nghi p Hà N i – Lu n văn th c s khoa h c Nông nghi p ……………………… 80 SQUARES SQUARES LN ============================================================================= CT$ 170178 170178 14.52 0.009 * RESIDUAL 70327.3 11721.2 * TOTAL (CORRECTED) 240505 34357.9 - TABLE OF MEANS FOR FACTORIAL EFFECTS FILE SLAND 26/ 8/** 10:38 PAGE ANH HUONG CUA NHIET DO DUNG DICH DEN DIEN TICH LA HUNG CHANH MEANS FOR EFFECT CT$ CT$ CT1 CT2 NOS 4 S LA 2726.02 2434.32 SE(N= 4) 54.1323 5%LSD 6DF 187.252 ANALYSIS OF VARIANCE SUMMARY TABLE FILE SLAND 26/ 8/** 10:38 PAGE ANH HUONG CUA NHIET DO DUNG DICH DEN DIEN TICH LA HUNG CHANH F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE S LA GRAND MEAN (N= 8) NO OBS 2580.2 STANDARD DEVIATION C OF V |CT$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 185.36 108.26 4.2 0.0091 | | | | BALANCED ANOVA FOR VARIATE 30NST FILE CKHOND 13/ 9/** 10:12 PAGE ANH HUONG CUA NHIET DO DUNG DICH DEN KHA NANG TICH LUY VAT CHAT KHO VARIATE V003 30NST LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 1.41120 1.41120 35.38 0.001 * RESIDUAL 239350 398917E-01 * TOTAL (CORRECTED) 1.65055 235793 - BALANCED ANOVA FOR VARIATE 60NST FILE CKHOND 13/ 9/** 10:12 PAGE ANH HUONG CUA NHIET DO DUNG DICH DEN KHA NANG TICH LUY VAT CHAT KHO VARIATE V004 60NST LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 3.68561 3.68561 15.16 0.008 * RESIDUAL 1.45908 243179 * TOTAL (CORRECTED) 5.14469 734955 BALANCED ANOVA FOR VARIATE 90NST FILE CKHOND 13/ 9/** 10:12 PAGE ANH HUONG CUA NHIET DO DUNG DICH DEN KHA NANG TICH LUY VAT CHAT KHO VARIATE V005 90NST LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 7.56605 7.56605 9.40 0.022 * RESIDUAL 4.83050 805084 - Trư ng ð i h c Nông nghi p Hà N i – Lu n văn th c s khoa h c Nông nghi p ……………………… 81 * TOTAL (CORRECTED) 12.3966 1.77094 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE CKHOND 13/ 9/** 10:12 PAGE ANH HUONG CUA NHIET DO DUNG DICH DEN KHA NANG TICH LUY VAT CHAT KHO MEANS FOR EFFECT CT$ CT$ CT1 CT2 NOS 4 30NST 7.15250 6.31250 60NST 11.3400 9.98250 90NST 28.1150 26.1700 SE(N= 4) 0.998645E-01 0.246566 0.448632 5%LSD 6DF 0.345447 0.852912 1.55189 ANALYSIS OF VARIANCE SUMMARY TABLE FILE CKHOND 13/ 9/** 10:12 PAGE ANH HUONG CUA NHIET DO DUNG DICH DEN KHA NANG TICH LUY VAT CHAT KHO F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE 30NST 60NST 90NST GRAND MEAN (N= 8) NO OBS 6.7325 10.661 27.143 STANDARD DEVIATION C OF V |CT$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 0.48559 0.19973 3.0 0.0012 0.85730 0.49313 4.6 0.0083 1.3308 0.89726 3.3 0.0219 | | | | nh hư ng c a phương th c tr ng tr t BALANCED ANOVA FOR VARIATE CH CAO FILE CCAOPT 1/ 9/** 16:54 PAGE ANH HUONG CUA PHUONG THUC TRONG TROT DEN CHIEU CAO HUNG CHANH VARIATE V003 CH CAO CAO CAO CAO LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 335.405 335.405 13.57 0.010 * RESIDUAL 148.275 24.7125 * TOTAL (CORRECTED) 483.680 69.0971 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE CCAOPT 1/ 9/** 16:54 PAGE ANH HUONG CUA PHUONG THUC TRONG TROT DEN CHIEU CAO HUNG CHANH MEANS FOR EFFECT CT$ CT$ dat canh NOS 4 CH CAO 66.2000 79.1500 SE(N= 4) 2.48558 5%LSD 6DF 8.59803 ANALYSIS OF VARIANCE SUMMARY TABLE FILE CCAOPT 1/ 9/** 16:54 PAGE ANH HUONG CUA PHUONG THUC TRONG TROT DEN CHIEU CAO HUNG CHANH F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE CH CAO GRAND MEAN (N= 8) NO OBS 72.675 STANDARD DEVIATION C OF V |CT$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 8.3125 4.9712 6.8 0.0105 BALANCED ANOVA FOR VARIATE SO LA FILE SOLAPP | | | | 1/ 9/** 16:48 Trư ng ð i h c Nông nghi p Hà N i – Lu n văn th c s khoa h c Nông nghi p ……………………… 82 PAGE ANH HUONG CUA PHUONG THUC TRONG TROT DEN SO LA HUNG CHANH VARIATE V003 SO LA LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 7375.05 7375.05 79.25 0.000 * RESIDUAL 558.379 93.0632 * TOTAL (CORRECTED) 7933.43 1133.35 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE SOLAPP 1/ 9/** 16:48 PAGE ANH HUONG CUA PHUONG THUC TRONG TROT DEN SO LA HUNG CHANH MEANS FOR EFFECT CT$ CT$ dat canh NOS 4 SO LA 190.950 251.675 SE(N= 4) 4.82346 5%LSD 6DF 16.6851 ANALYSIS OF VARIANCE SUMMARY TABLE FILE SOLAPP 1/ 9/** 16:48 PAGE ANH HUONG CUA PHUONG THUC TRONG TROT DEN SO LA HUNG CHANH F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE SO LA GRAND MEAN (N= 8) NO OBS 221.31 STANDARD DEVIATION C OF V |CT$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 33.665 9.6469 4.4 0.0002 | | | | BALANCED ANOVA FOR VARIATE 30NST FILE LAI30 13/ 9/** 9:21 PAGE ANH HUONG CUA PHUONG THUC TRONG TROT DEN DIEN TICH LA HUNG CHANH VARIATE V003 30NST LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 52703.7 52703.7 37.25 0.001 * RESIDUAL 8488.85 1414.81 * TOTAL (CORRECTED) 61192.5 8741.79 BALANCED ANOVA FOR VARIATE 60NST FILE LAI30 13/ 9/** 9:21 PAGE ANH HUONG CUA PHUONG THUC TRONG TROT DEN DIEN TICH LA HUNG CHANH VARIATE V004 60NST LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 162419E+07 162419E+07 566.85 0.000 * RESIDUAL 17191.7 2865.28 * TOTAL (CORRECTED) 164138E+07 234483 - BALANCED ANOVA FOR VARIATE 90NST FILE LAI30 13/ 9/** 9:21 PAGE ANH HUONG CUA PHUONG THUC TRONG TROT DEN DIEN TICH LA HUNG CHANH VARIATE V005 90NST Trư ng ð i h c Nông nghi p Hà N i – Lu n văn th c s khoa h c Nông nghi p ……………………… 83 LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 223809E+07 223809E+07 728.77 0.000 * RESIDUAL 18426.4 3071.07 * TOTAL (CORRECTED) 225652E+07 322360 TABLE OF MEANS FOR FACTORIAL EFFECTS FILE LAI30 13/ 9/** 9:21 PAGE ANH HUONG CUA PHUONG THUC TRONG TROT DEN DIEN TICH LA HUNG CHANH MEANS FOR EFFECT CT$ CT$ dat canh NOS 4 30NST 875.217 1037.55 60NST 1054.59 1955.75 90NST 1799.82 2857.68 SE(N= 4) 18.8070 26.7642 27.7086 5%LSD 6DF 65.0563 92.5815 95.8486 ANALYSIS OF VARIANCE SUMMARY TABLE FILE LAI30 13/ 9/** 9:21 PAGE ANH HUONG CUA PHUONG THUC TRONG TROT DEN DIEN TICH LA HUNG CHANH F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE 30NST 60NST 90NST GRAND MEAN (N= 8) NO OBS 956.38 1505.2 2328.7 STANDARD DEVIATION C OF V |CT$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 93.498 37.614 3.9 0.0011 484.23 53.528 3.6 0.0000 567.77 55.417 2.4 0.0000 | | | | BALANCED ANOVA FOR VARIATE 30NST FILE CKHO 13/ 9/** 9:42 PAGE ANH HUONG CUA PHUONG THUC TRONG TROT DEN SU TICH LUY VAT CHAT KHO VARIATE V003 30NST LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 5.12000 5.12000 123.27 0.000 * RESIDUAL 249201 415335E-01 * TOTAL (CORRECTED) 5.36920 767029 - BALANCED ANOVA FOR VARIATE 60NST FILE CKHO 13/ 9/** 9:42 PAGE ANH HUONG CUA PHUONG THUC TRONG TROT DEN SU TICH LUY VAT CHAT KHO VARIATE V004 60NST LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= CT$ 20.2566 20.2566 243.92 0.000 * RESIDUAL 498275 830459E-01 * TOTAL (CORRECTED) 20.7549 2.96498 BALANCED ANOVA FOR VARIATE 90NST FILE CKHO 13/ 9/** 9:42 PAGE ANH HUONG CUA PHUONG THUC TRONG TROT DEN SU TICH LUY VAT CHAT KHO VARIATE V005 90NST LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER Trư ng ð i h c Nông nghi p Hà N i – Lu n văn th c s khoa h c Nông nghi p ……………………… 84 SQUARES SQUARES LN ============================================================================= CT$ 250.208 250.208 378.53 0.000 * RESIDUAL 3.96600 661000 * TOTAL (CORRECTED) 254.174 36.3106 - TABLE OF MEANS FOR FACTORIAL EFFECTS FILE CKHO 13/ 9/** 9:42 PAGE ANH HUONG CUA PHUONG THUC TRONG TROT DEN SU TICH LUY VAT CHAT KHO MEANS FOR EFFECT CT$ CT$ dat canh NOS 4 30NST 4.47000 6.07000 60NST 6.59000 9.77250 90NST 14.3325 25.5175 SE(N= 4) 0.101899 0.144088 0.406510 5%LSD 6DF 0.352484 0.498425 1.40618 ANALYSIS OF VARIANCE SUMMARY TABLE FILE CKHO 13/ 9/** 9:42 PAGE ANH HUONG CUA PHUONG THUC TRONG TROT DEN SU TICH LUY VAT CHAT KHO F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL SECTION - VARIATE 30NST 60NST 90NST GRAND MEAN (N= 8) NO OBS 5.2700 8.1812 19.925 STANDARD DEVIATION C OF V |CT$ SD/MEAN | BASED ON BASED ON % | TOTAL SS RESID SS | 0.87580 0.20380 3.9 0.0001 1.7219 0.28818 3.5 0.0000 6.0258 0.81302 4.1 0.0000 | | | | Trư ng ð i h c Nông nghi p Hà N i – Lu n văn th c s khoa h c Nông nghi p ……………………… 85 ... VÀ ðÀO T O TRƯ NG ð I H C NÔNG NGHI P HÀ N I NGUY N XUÂN HƯƠNG NGHIÊN C U NHÂN GI NG VÔ TÍNH CÂY HÚNG CHANH Nð (COLEUS FORSKOHLII) B NG K THU T NUÔI C Y MÔ VÀ PHƯƠNG PHÁP GIÂM... a húng chanh n ð ñư c nhân b ng giâm ng n h th ng khí canh 2.4 Phương pháp nghiên c u 2.4.1 Phương pháp b trí thí nghi m 2.4.1.1 N i dung 1: Nghiên c u nhân gi ng húng chanh n ð b ng k thu t nuôi. .. a ñi m nghiên c u 21 2.3 N i dung nghiên c u 21 2.3.1 Nghiên c u nhân gi ng húng chanh n ð b ng k thu t nuôi c y mô 21 2.3.2 Nghiên c u k thu t tr ng húng chanh n ð b ng phương pháp khí canh Trư