[...]... Reaction) 11 1. 11 Sudan Black B Stain 13 1. 12 Cytochemical Determination of Peroxidase 13 1. 13 Hydrolases 13 1. 13 .1 Cytochemical Determination of Leukocyte Alkaline Phosphatase (LAP) in Blood Smears 13 1. 13.2 Cytochemical Determination of Acid Phosphatase 14 1. 13.3 Detection of Esterases with Naphthyl Acetate or Naphthyl Butyrate (”Neutral Esterases”) 14 , Acid Esterase (ANAE) 15 1. 13.4 Naphthol AS-D Chloroacetate... Leukemia (CNL) 15 7 Chronic Eosinophilic Leukemia (CEL) and the Hypereosinophilic Syndrome (HES) 15 7 5.9 Myelodysplastic Syndromes (MDS) 69 69 11 9 12 7 12 9 13 2 15 8 5 .10 Acute Leukemias 18 0 5 .10 .1 Acute Myeloid Leukemia (AML) 18 3 5 .10 .2 Acute Lymphoblastic Leukemia (ALL) (Fig 11 5 a – d) 5 .11 10 1 265 Neoplasias of Tissue Mast Cells (Malignant Mastocytoses) 286 IV ... Congenital Dyserythropoietic Anemias 10 1 Synartesis 10 5 5.3 5.3 .1 5.3.2 5.3.3 5.3.4 Reactive Blood and Bone Marrow Changes 10 7 Agranulocytosis 11 4 Kostmann Syndrome 11 7 Thrombocytopenias and Thrombocytopathies 11 7 Pseudothrombopenia 11 7 5.4 Bone Marrow Aplasias (Panmyelopathies) 5.5 5.5 .1 5.5.2 5.5.3 Storage Diseases 12 2 Gaucher Disease 12 2 Niemann-Pick Disease 12 5 Glycogen Storage Disease Type II... Chloroacetate Esterase (CE) 15 1. 14 Appendix 16 2 Immunocytochemical Detection of Cell-Surface and Intracellular Antigens 18 3 Staining Methods for the Detection of Blood Parasites 19 3 .1 “Thick Smear” Method 19 3.2 Bartonellosis 19 3.3 Detection of Blood Parasites in Bone Marrow Smears 19 3.4 Toxoplasmosis 19 3.5 Microfiliariasis 19 3.6 Mycobacterium Species (M tuberculosis, M leprae) 19 II 8 Chapter II ·... 4 .1 4 .1. 1 4 .1. 2 4 .1. 3 4 .1. 4 4 .1. 5 4 .1. 6 4 .1. 7 4 .1. 8 Light Microscopic Morphology and Cytochemistry 28 Cells of Erythropoiesis (Fig 4 a – f) 28 Granulocytopoiesis and Mast Cells (Tissue Basophils) 38 Degenerate Forms, Toxic Changes and Artifacts (Fig 9 a – d) 44 Congenital Anomalies of Granulocytopoiesis (Fig 10 a – f) 46 Cells of the Monocyte-Macrophage System (Fig 13 a – h) 54 Megakaryocytes (Fig 15 ... Mayer’s Acid Hemalum Nuclear Stain 8 1. 4 Heilmeyer’s Reticulocyte Stain 8 1. 5 Heinz Body Test of Beutler 8 1. 6 Nile Blue Sulfate Stain 9 1. 7 Kleihauer-Betke Stain for Demonstrating Fetal Hemoglobin in Red Blood Cells 9 1. 8 Kleihauer-Betke Stain for Demonstrating Methemoglobin-Containing Cells in Blood Smears 10 1. 9 Berlin Blue Iron Stain 10 1. 10 Cytochemical Determination of Glycogen in Blood Cells by the... Porwit-MacDonald A (2002) Antigen expression patterns reflecting genotype of acute leukemias Leukemia 16 : 12 33 – 12 58) 19 1 · Staining Methods for the Morphologic and Cytochemical Differentiation of Cells 3 Staining Methods for the Detection of Blood Parasites1 3 .1 “Thick Smear” Method One drop of blood is placed on a slide and spread with the edge of a second slide to cover an area the size of a dime... diaminobenzidine (more often used) is converted, in the presence of peroxide, from the leuko form into a high-polymer form that is detectable by cytochemical staining Reagents  Fixative: methanol + 37 % formalin (10 : 1)  DAB solution: 5 mg diaminobenzidine tetrahydrochloride in 20 mL of 0.05 mol/L tris-HCl buffer (pH 7.6) with 50 lL of 1 % H2O2 added  Tris-HCl: 50 mL of solution A (12 1 .14 g trishydroxymethylaminomethane... acidity for staining is to use a pH 7.3 buffer solution (22.3 mL of 1/ 15 mol/L KH2PO4 + 77.7 mL of 1/ 15 mol/L Na2HPO4) instead of water 1. 2 Undritz Toluidine Blue Stain for Basophils Reagent Saturated toluidine blue-methanol: dissolve 1 g toluidine blue in 10 0 mL methanol The solution will keep indefinitely Method Fix and stain the air-dried smears on the staining rack by covering with the toluidine... mg of L-tartaric acid to 30 mL of the staining solution, then analyze as described for acid phosphatase Fast garnet GBC can be used as a coupling salt instead of the pararosaniline solution This requires the following modifications in the staining solution: Dissolve 10 mg naphthol-AS-BI phosphate in 0.5 mL dimethylformamide, and add 0 .1 mol/L acetate buffer pH 5.0 to make 10 mL Dissolve 10 – 15 mg of . Test (PAS Reaction) 11 1. 11 Sudan Black B Stain 13 1. 12 Cytochemical Determination of Peroxidase 13 1. 13 Hydrolases 13 1. 14 Appendix 16 XI XII 2 Immunocytochemical Detection of Cell-Surface and Intracellular Antigens. Determination of Peroxidase 13 1. 13 Hydrolases 13 1. 13 .1 Cytochemical Determination of Leukocyte Alkaline Phosphatase (LAP) in Blood Smears 13 1. 13.2 Cytochemical Determination of Acid Phosphatase 14 1. 13.3. ed. 19 72 3rd ed. 19 78 4th ed. 19 87 5th ed. 19 99 Japanese edition Rinsho Ketsuekigaku Atlas ª Springer-Verlag Tokyo, 19 89 Translated by: Terry C. Telger, Fort Worth, Texas, USA ISBN 3-5 4 0-2 10 13-X