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Với mong muốn phát triển hơn nữa khả năng nuôi trồng và ứng dụng nấm vân chi trong ngành dược phẩm, chúng tôi thực hiện đề tài: Khảo sát sinh trưởng một chủng nấm vân chi đen Trametes versicolor có nguồn gốc từ Trung Quốc.
PHỤ LỤC PHỤ LỤC 1: Kết quả phân tích thống kê các thí nghiệm môi trường nhân giống cấp 1 One-Way Analysis of Variance ------------------------------------------------------------------------------ Data: THDDUONG.ketqua Level codes: THDDUONG.nt Labels: Means plot: LSD Confidence level: 95 Range test: LSD Analysis of variance -------------------------------------------------------------------------------- Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level -------------------------------------------------------------------------------- Between groups 953.80467 4 238.45117 48.819 .0000 Within groups 48.84405 10 4.88441 -------------------------------------------------------------------------------- Total (corrected) 1002.6487 14 0 missing value(s) have been excluded. Table of means for THDDUONG.ketqua by THDDUONG.nt -------------------------------------------------------------------------------- Stnd. Error Stnd. Error 95 % LSD Level Count Average (internal) (pooled s) intervals for mean -------------------------------------------------------------------------------- psa 3 79.235000 1.2928554 1.2759839 77.224110 81.245890 cra 3 60.220000 .3878574 1.2759839 58.209110 62.230890 bth 3 78.113333 1.7888202 1.2759839 76.102444 80.124223 dua 3 82.806667 .7798789 1.2759839 80.795777 84.817556 gia 3 78.793333 1.5845118 1.2759839 76.782444 80.804223 -------------------------------------------------------------------------------- Total 15 75.833667 .5706374 .5706374 74.934369 76.732964 Multiple range analysis for THDDUONG.ketqua by THDDUONG.nt -------------------------------------------------------------------------------- Method: 95 Percent LSD Level Count Average Homogeneous Groups -------------------------------------------------------------------------------- cra 3 60.220000 X bth 3 78.113333 X gia 3 78.793333 XX psa 3 79.235000 XX dua 3 82.806667 X -------------------------------------------------------------------------------- contrast difference limits psa - cra 19.0150 4.02178 * psa - bth 1.12167 4.02178 psa - dua -3.57167 4.02178 psa - gia 0.44167 4.02178 cra - bth -17.8933 4.02178 * cra - dua -22.5867 4.02178 * cra - gia -18.5733 4.02178 * bth - dua -4.69333 4.02178 * bth - gia -0.68000 4.02178 dua - gia 4.01333 4.02178 -------------------------------------------------------------------------------- * denotes a statistically significant difference. One-Way Analysis of Variance -------------------------------------------------------------------------------- Data: THNHDO.ketqua Level codes: THNHDO.nt Labels: Means plot: LSD Confidence level: 95 Range test: LSD Analysis of variance -------------------------------------------------------------------------------- Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level -------------------------------------------------------------------------------- Between groups 51.861600 1 51.861600 7.880 .0485 Within groups 26.325933 4 6.581483 -------------------------------------------------------------------------------- Total (corrected) 78.187533 5 0 missing value(s) have been excluded. Table of means for THNHDO.ketqua by THNHDO.nt -------------------------------------------------------------------------------- Stnd. Error Stnd. Error 95 % LSD Level Count Average (internal) (pooled s) intervals for mean -------------------------------------------------------------------------------- 27 3 72.233333 1.0898522 1.4811576 69.324430 75.142237 30 3 78.113333 1.7888202 1.4811576 75.204430 81.022237 -------------------------------------------------------------------------------- Total 6 75.173333 1.0473366 1.0473366 73.116428 77.230239 Multiple range analysis for THNHDO.ketqua by THNHDO.nt -------------------------------------------------------------------------------- Method: 95 Percent LSD Level Count Average Homogeneous Groups -------------------------------------------------------------------------------- 27 3 72.233333 X 30 3 78.113333 X -------------------------------------------------------------------------------- contrast difference limits 27 - 30 -5.88000 5.81781 * -------------------------------------------------------------------------------- * denotes a statistically significant difference. One-Way Analysis of Variance -------------------------------------------------------------------------------- Data: THACHPH.ketqua Level codes: THACHPH.nt Labels: Means plot: LSD Confidence level: 95 Range test: LSD Analysis of variance -------------------------------------------------------------------------------- Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level -------------------------------------------------------------------------------- Between groups 1460.7687 4 365.19218 42.369 .0000 Within groups 86.1927 10 8.61927 -------------------------------------------------------------------------------- Total (corrected) 1546.9614 14 0 missing value(s) have been excluded. Table of means for THACHPH.ketqua by THACHPH.nt -------------------------------------------------------------------------------- Stnd. Error Stnd. Error 95 % LSD Level Count Average (internal) (pooled s) intervals for mean -------------------------------------------------------------------------------- 5 3 74.376000 1.8895011 1.6950189 71.704731 77.047269 5.5 3 75.640000 1.8620150 1.6950189 72.968731 78.311269 6 3 70.100000 2.1383717 1.6950189 67.428731 72.771269 6.5 3 61.183333 1.1555999 1.6950189 58.512064 63.854602 7 3 49.151667 1.1916737 1.6950189 46.480398 51.822936 -------------------------------------------------------------------------------- Total 15 66.090200 .7580355 .7580355 64.895572 67.284828 08/10/05 12:22:20 PM Page 1 Multiple range analysis for THACHPH.ketqua by THACHPH.nt -------------------------------------------------------------------------------- Method: 95 Percent LSD Level Count Average Homogeneous Groups -------------------------------------------------------------------------------- 7 3 49.151667 X 6.5 3 61.183333 X 6 3 70.100000 X 5 3 74.376000 XX 5.5 3 75.640000 X -------------------------------------------------------------------------------- contrast difference limits 5 - 5.5 -1.26400 5.34254 5 - 6 4.27600 5.34254 5 - 6.5 13.1927 5.34254 * 5 - 7 25.2243 5.34254 * 5.5 - 6 5.54000 5.34254 * 5.5 - 6.5 14.4567 5.34254 * 5.5 - 7 26.4883 5.34254 * 6 - 6.5 8.91667 5.34254 * 6 - 7 20.9483 5.34254 * 6.5 - 7 12.0317 5.34254 * -------------------------------------------------------------------------------- * denotes a statistically significant difference. PHỤ LỤC 2: Kết quả phân tích thống kê các môi trường nhân giống cấp 2 One-Way Analysis of Variance -------------------------------------------------------------------------------- Data: MTNGTG.ketqua Level codes: MTNGTG.nt Labels: Means plot: LSD Confidence level: 95 Range test: LSD Analysis of variance -------------------------------------------------------------------------------- Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level -------------------------------------------------------------------------------- Between groups 2236.2254 8 279.52818 35.786 .0000 Within groups 140.5981 18 7.81100 -------------------------------------------------------------------------------- Total (corrected) 2376.8235 26 0 missing value(s) have been excluded. Table of means for MTNGTG.ketqua by MTNGTG.nt -------------------------------------------------------------------------------- Stnd. Error Stnd. Error 95 % LSD Level Count Average (internal) (pooled s) intervals for mean -------------------------------------------------------------------------------- 1 3 92.94667 2.3487325 1.6135885 90.548971 95.34436 2 3 102.16667 .2531359 1.6135885 99.768971 104.56436 3 3 94.91667 .7936694 1.6135885 92.518971 97.31436 4 3 82.05667 1.7324966 1.6135885 79.658971 84.45436 5 3 100.91667 1.0999596 1.6135885 98.518971 103.31436 6 3 94.38667 .9433333 1.6135885 91.988971 96.78436 7 3 77.89000 2.3120193 1.6135885 75.492305 80.28770 8 3 75.41667 2.1032382 1.6135885 73.018971 77.81436 9 3 92.14000 1.5336558 1.6135885 89.742305 94.53770 -------------------------------------------------------------------------------- Total 27 90.31519 .5378628 .5378628 89.515953 91.11442 Multiple range analysis for MTNGTG.ketqua by MTNGTG.nt -------------------------------------------------------------------------------- Method: 95 Percent LSD Level Count Average Homogeneous Groups -------------------------------------------------------------------------------- 8 3 75.41667 X 7 3 77.89000 XX 4 3 82.05667 X 9 3 92.14000 X 1 3 92.94667 X 6 3 94.38667 X 3 3 94.91667 X 5 3 100.91667 X 2 3 102.16667 X -------------------------------------------------------------------------------- contrast difference limits 1 - 2 -9.22000 4.79539 * 1 - 3 -1.97000 4.79539 1 - 4 10.8900 4.79539 * 1 - 5 -7.97000 4.79539 * 1 - 6 -1.44000 4.79539 1 - 7 15.0567 4.79539 * 1 - 8 17.5300 4.79539 * 1 - 9 0.80667 4.79539 2 - 3 7.25000 4.79539 * 2 - 4 20.1100 4.79539 * 2 - 5 1.25000 4.79539 2 - 6 7.78000 4.79539 * 2 - 7 24.2767 4.79539 * 2 - 8 26.7500 4.79539 * 2 - 9 10.0267 4.79539 * 3 - 4 12.8600 4.79539 * 3 - 5 -6.00000 4.79539 * 3 - 6 0.53000 4.79539 3 - 7 17.0267 4.79539 * 3 - 8 19.5000 4.79539 * 3 - 9 2.77667 4.79539 4 - 5 -18.8600 4.79539 * 4 - 6 -12.3300 4.79539 * 4 - 7 4.16667 4.79539 4 - 8 6.64000 4.79539 * 4 - 9 -10.0833 4.79539 * 5 - 6 6.53000 4.79539 * 5 - 7 23.0267 4.79539 * 5 - 8 25.5000 4.79539 * 5 - 9 8.77667 4.79539 * 6 - 7 16.4967 4.79539 * 6 - 8 18.9700 4.79539 * 6 - 9 2.24667 4.79539 7 - 8 2.47333 4.79539 7 - 9 -14.2500 4.79539 * 8 - 9 -16.7233 4.79539 * -------------------------------------------------------------------------------- * denotes a statistically significant difference. PHỤ LỤC 3: Kết quả phân tích thống kê các thí nghiệm về khả năng tạo sinh khối nấm vân chi trong môi trường lỏng One-Way Analysis of Variance -------------------------------------------------------------------------------- Data: MTLTN1.ketqua Level codes: MTLTN1.nt Labels: Means plot: LSD Confidence level: 95 Range test: LSD Analysis of variance -------------------------------------------------------------------------------- Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level -------------------------------------------------------------------------------- Between groups .2255989 2 .1127994 234.480 .0000 Within groups .0028864 6 .0004811 -------------------------------------------------------------------------------- Total (corrected) .2284852 8 0 missing value(s) have been excluded. Table of means for MTLTN1.ketqua by MTLTN1.nt -------------------------------------------------------------------------------- Stnd. Error Stnd. Error 95 % LSD Level Count Average (internal) (pooled s) intervals for mean -------------------------------------------------------------------------------- ps 3 .2101333 .0024258 .0126631 .1882166 .2320500 cra 3 .0459333 .0026934 .0126631 .0240166 .0678500 bth 3 .4323000 .0216315 .0126631 .4103833 .4542167 -------------------------------------------------------------------------------- Total 9 .2294556 .0073110 .0073110 .2168019 .2421092 Multiple range analysis for MTLTN1.ketqua by MTLTN1.nt -------------------------------------------------------------------------------- Method: 95 Percent LSD Level Count Average Homogeneous Groups -------------------------------------------------------------------------------- cra 3 .0459333 X ps 3 .2101333 X bth 3 .4323000 X -------------------------------------------------------------------------------- contrast difference limits ps - cra 0.16420 0.04383 * ps - bth -0.22217 0.04383 * cra - bth -0.38637 0.04383 * -------------------------------------------------------------------------------- * denotes a statistically significant difference. One-Way Analysis of Variance -------------------------------------------------------------------------------- Data: MTL10MT.ketqua Level codes: MTL10MT.nt Labels: Means plot: LSD Confidence level: 95 Range test: LSD Analysis of variance -------------------------------------------------------------------------------- Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level -------------------------------------------------------------------------------- Between groups .1729553 9 .0192173 25.185 .0000 Within groups .0152606 20 .0007630 -------------------------------------------------------------------------------- Total (corrected) .1882159 29 0 missing value(s) have been excluded. Table of means for MTL10MT.ketqua by MTL10MT.nt -------------------------------------------------------------------------------- Stnd. Error Stnd. Error 95 % LSD Level Count Average (internal) (pooled s) intervals for mean -------------------------------------------------------------------------------- 1 3 .1581333 .0371602 .0159481 .1346042 .1816625 2 3 .2276000 .0108523 .0159481 .2040708 .2511292 3 3 .2432667 .0090497 .0159481 .2197375 .2667958 4 3 .1973000 .0100022 .0159481 .1737708 .2208292 5 3 .2047333 .0104539 .0159481 .1812042 .2282625 6 3 .2074000 .0076559 .0159481 .1838708 .2309292 7 3 .3402000 .0090163 .0159481 .3166708 .3637292 8 3 .2332000 .0094599 .0159481 .2096708 .2567292 9 3 .2289000 .0074987 .0159481 .2053708 .2524292 10 3 .4323000 .0216315 .0159481 .4087708 .4558292 -------------------------------------------------------------------------------- Total 30 .2473033 .0050432 .0050432 .2398628 .2547439 Multiple range analysis for MTL10MT.ketqua by MTL10MT.nt -------------------------------------------------------------------------------- Method: 95 Percent LSD Level Count Average Homogeneous Groups -------------------------------------------------------------------------------- 1 3 .1581333 X 4 3 .1973000 XX 5 3 .2047333 XX 6 3 .2074000 X 2 3 .2276000 X 9 3 .2289000 X 8 3 .2332000 X 3 3 .2432667 X 7 3 .3402000 X 10 3 .4323000 X -------------------------------------------------------------------------------- contrast difference +/- limits 1 - 2 -0.06947 0.04706 * 1 - 3 -0.08513 0.04706 * 1 - 4 -0.03917 0.04706 1 - 5 -0.04660 0.04706 1 - 6 -0.04927 0.04706 * 1 - 7 -0.18207 0.04706 * 1 - 8 -0.07507 0.04706 * 1 - 9 -0.07077 0.04706 * 1 - 10 -0.27417 0.04706 * 2 - 3 -0.01567 0.04706 2 - 4 0.03030 0.04706 2 - 5 0.02287 0.04706 2 - 6 0.02020 0.04706 2 - 7 -0.11260 0.04706 * 2 - 8 -0.00560 0.04706 2 - 9 -0.00130 0.04706 2 - 10 -0.20470 0.04706 * 3 - 4 0.04597 0.04706 3 - 5 0.03853 0.04706 3 - 6 0.03587 0.04706 3 - 7 -0.09693 0.04706 * 3 - 8 0.01007 0.04706 3 - 9 0.01437 0.04706 3 - 10 -0.18903 0.04706 * 4 - 5 -0.00743 0.04706 4 - 6 -0.01010 0.04706 4 - 7 -0.14290 0.04706 * 4 - 8 -0.03590 0.04706 4 - 9 -0.03160 0.04706 4 - 10 -0.23500 0.04706 * 5 - 6 -0.00267 0.04706 5 - 7 -0.13547 0.04706 * 5 - 8 -0.02847 0.04706 5 - 9 -0.02417 0.04706 5 - 10 -0.22757 0.04706 * 6 - 7 -0.13280 0.04706 * 6 - 8 -0.02580 0.04706 6 - 9 -0.02150 0.04706 6 - 10 -0.22490 0.04706 * 7 - 8 0.10700 0.04706 * 7 - 9 0.11130 0.04706 * 7 - 10 -0.09210 0.04706 * 8 - 9 0.00430 0.04706 8 - 10 -0.19910 0.04706 * 9 - 10 -0.20340 0.04706 * -------------------------------------------------------------------------------- * denotes a statistically significant difference. PHỤ LỤC 4: Kết quả phân tích thống kê các thí nghiệm ly trích hợp chất polysaccharide thô từ sinh khối nấm vân chi One-Way Analysis of Variance -------------------------------------------------------------------------------- Data: TLTN1.KETQUA Level codes: TLTN1.NT Labels: Means plot: LSD Confidence level: 95 Range test: LSD Analysis of variance -------------------------------------------------------------------------------- Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level -------------------------------------------------------------------------------- Between groups 240.89087 2 120.44543 1237.594 .0000 Within groups .58393 6 .09732 -------------------------------------------------------------------------------- Total (corrected) 241.47480 8 0 missing value(s) have been excluded. Table of means for TLTN1.KETQUA by TLTN1.NT -------------------------------------------------------------------------------- Stnd. Error Stnd. Error 95 % LSD Level Count Average (internal) (pooled s) intervals for mean -------------------------------------------------------------------------------- PSA 3 10.783333 .1812304 .1801131 10.471602 11.095065 BTH 3 11.156667 .2539247 .1801131 10.844935 11.468398 CRA 3 .000000 .0000000 .1801131 -.311731 .311731 -------------------------------------------------------------------------------- Total 9 7.313333 .1039884 .1039884 7.133355 7.493311 Multiple range analysis for TLTN1.KETQUA by TLTN1.NT -------------------------------------------------------------------------------- Method: 95 Percent LSD Level Count Average Homogeneous Groups -------------------------------------------------------------------------------- CRA 3 .000000 X PSA 3 10.783333 X BTH 3 11.156667 X -------------------------------------------------------------------------------- contrast difference limits PSA - BTH -0.37333 0.62346 PSA - CRA 10.7833 0.62346 * BTH - CRA 11.1567 0.62346 * -------------------------------------------------------------------------------- * denotes a statistically significant difference. One-Way Analysis of Variance -------------------------------------------------------------------------------- Data: TL10MT.ketqua Level codes: TL10MT.nt Labels: Means plot: LSD Confidence level: 95 Range test: LSD Analysis of variance -------------------------------------------------------------------------------- Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level -------------------------------------------------------------------------------- Between groups 272.88421 9 30.320468 10.527 .0000 Within groups 57.60673 20 2.880337 -------------------------------------------------------------------------------- Total (corrected) 330.49095 29 0 missing value(s) have been excluded. Table of means for TL10MT.ketqua by TL10MT.nt -------------------------------------------------------------------------------- Stnd. Error Stnd. Error 95 % LSD Level Count Average (internal) (pooled s) intervals for mean -------------------------------------------------------------------------------- 1 3 11.163333 .3595058 .9798532 9.717701 12.608966 2 3 11.650000 .1985783 .9798532 10.204368 13.095632 3 3 12.376667 .2509537 .9798532 10.931034 13.822299 4 3 11.676667 1.0785999 .9798532 10.231034 13.122299 5 3 14.016667 .8871365 .9798532 12.571034 15.462299 6 3 14.546667 .3919325 .9798532 13.101034 15.992299 7 3 12.960000 1.0759802 .9798532 11.514368 14.405632 8 3 21.753333 1.9380517 .9798532 20.307701 23.198966 9 3 15.053333 1.5123528 .9798532 13.607701 16.498966 10 3 11.156667 .2539247 .9798532 9.711034 12.602299 -------------------------------------------------------------------------------- Total 30 13.635333 .3098568 .3098568 13.178184 14.092482 Multiple range analysis for TL10MT.ketqua by TL10MT.nt -------------------------------------------------------------------------------- Method: 95 Percent LSD Level Count Average Homogeneous Groups -------------------------------------------------------------------------------- 10 3 11.156667 X 1 3 11.163333 X 2 3 11.650000 X 4 3 11.676667 XX 3 3 12.376667 XXX 7 3 12.960000 XXX 5 3 14.016667 XXX 6 3 14.546667 XX 9 3 15.053333 X 8 3 21.753333 X -------------------------------------------------------------------------------- contrast difference limits 1 - 2 -0.48667 2.89126 1 - 3 -1.21333 2.89126 1 - 4 -0.51333 2.89126 1 - 5 -2.85333 2.89126 1 - 6 -3.38333 2.89126 * 1 - 7 -1.79667 2.89126 1 - 8 -10.5900 2.89126 * 1 - 9 -3.89000 2.89126 * 1 - 10 0.00667 2.89126 2 - 3 -0.72667 2.89126 2 - 4 -0.02667 2.89126 [...]... acid amin có trong nước dừa Bảng 7.1: Hàm lượng các vitamin có trong nước dừa Vitamin Hàm lượng (μg/ml) Khoáng vi lượng Hàm lượng (μg/100ml) Acid ascorbic 2200 – 3700 K 3,12 Acid nicotinic 0,64 Na 1,50 Acid pantothenic 0,52 Ca 2,09 Acid folic 0,003 Mg 3,00 Biotin 0,02 Fe 0,01 Riboflavin 0,01 Cu 0,04 Thiamine dạng vết (trace) S 3,40 Pyridoxine dạng vết (trace) P 3,70 Bảng 7.2: Hàm lượng acid amin có trong . tích thống kê các thí nghiệm ly trích hợp chất polysaccharide thô từ sinh khối nấm vân chi One-Way Analysis of Variance . LỤC 3: Kết quả phân tích thống kê các thí nghiệm về khả năng tạo sinh khối nấm vân chi trong môi trường lỏng One-Way Analysis
