Multiple range analysis for DR14DAY.DAIRE by DR14DAY.NT Method: 95 Percent LSD Level Count Average Homogeneous Groups 1 27 2.2222222 X 2 27 7.5925926 X contrast difference +/- limits 1 - 2 -5.37037 0.29520 * * denotes a statistically significant difference. 21 ngày Analysis of variance Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level Between groups 4.166667 1 4.1666667 7.927 .0069 Within groups 27.333333 52 .5256410 Total (corrected) 31.500000 53 0 missing value(s) have been excluded. Multiple range analysis for DR21DAY.DAIRE by DR21DAY.NT Method: 95 Percent LSD Level Count Average Homogeneous Groups 1 27 5.2222222 X 2 27 5.7777778 X contrast difference limits 1 - 2 0.55556 0.39605 * * denotes a statistically significant difference. 28 ngày Analysis of variance Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level Between groups 266.66667 1 266.66667 216.166 .0000 Within groups 64.14815 52 1.23362 Total (corrected) 330.81481 53 0 missing value(s) have been excluded Multiple range analysis for DR28DAY.DAIRE by DR28DAY.NT Method: 95 Percent LSD Level Count Average Homogeneous Groups 1 27 5.629630 X 2 27 10.074074 X contrast difference +/- limits 1 - 2 -4.44444 0.60673 * * denotes a statistically significant difference. 35 ngày Analysis of variance Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level Between groups 317.79630 1 317.79630 157.108 .0000 Within groups 105.18519 52 2.02279 Total (corrected) 422.98148 53 0 missing value(s) have been excluded. Multiple range analysis for DR35DAY.daire by DR35DAY.NT Method: 95 Percent LSD Level Count Average Homogeneous Groups 1 27 7.592593 X 2 27 12.444444 X contrast difference limits 1 - 2 -4.85185 0.77692 * * denotes a statistically significant difference. 4. XỬ LÝ SỐ LIỆU CÂY TRUỜNG XUÂN HOA IN VITRO TRÊN MÔI TRƯỜNG CHỨA NAA CAO CÂY TRÊN MOI TRUƠNG CÓ NAA Analysis of variance Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level Between groups 1124.938 5 224.98765 2.785 .0194 Within groups 12603.704 156 80.79297 Total (corrected) 13728.642 161 0 missing value(s) have been excluded. Multiple range analysis for CCNAA.Caocay by CCNAA.NT Method: 95 Percent LSD Level Count Average Homogeneous Groups 6 27 49.296296 X 5 27 49.518519 X 2 27 51.814815 XX 1 27 51.851852 XX 3 27 55.666667 X 4 27 55.925926 X contrast difference limits 1 - 2 0.03704 4.83333 1 - 3 -3.81481 4.83333 1 - 4 -4.07407 4.83333 1 - 5 2.33333 4.83333 1 - 6 2.55556 4.83333 2 - 3 -3.85185 4.83333 2 - 4 -4.11111 4.83333 2 - 5 2.29630 4.83333 2 - 6 2.51852 4.83333 3 - 4 -0.25926 4.83333 3 - 5 6.14815 4.83333 * 3 - 6 6.37037 4.83333 * 4 - 5 6.40741 4.83333 * 4 - 6 6.62963 4.83333 * 5 - 6 0.22222 4.83333 * denotes a statistically significant difference. SỐ RỄ TRÊN MOI TRUƠNG CÓ NAA Analysis of variance Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level Between groups 216.27160 5 43.254321 147.401 .0000 Within groups 45.77778 156 .293447 Total (corrected) 262.04938 161 0 missing value(s) have been excluded. Multiple range analysis for SRNÁA.SORE by SRNAA.NT Method: 95 Percent LSD Level Count Average Homogeneous Groups 6 27 .6666667 X 5 27 1.9259259 X 1 27 2.9259259 X 2 27 3.0000000 X 4 27 3.6666667 X 3 27 4.1851852 X contrast difference limits 1 - 2 -0.07407 0.29129 1 - 3 -1.25926 0.29129 * 1 - 4 -0.74074 0.29129 * 1 - 5 1.00000 0.29129 * 1 - 6 2.25926 0.29129 * 2 - 3 -1.18519 0.29129 * 2 - 4 -0.66667 0.29129 * 2 - 5 1.07407 0.29129 * 2 - 6 2.33333 0.29129 * 3 - 4 0.51852 0.29129 * 3 - 5 2.25926 0.29129 * 3 - 6 3.51852 0.29129 * 4 - 5 1.74074 0.29129 * 4 - 6 3.00000 0.29129 * 5 - 6 1.25926 0.29129 * * denotes a statistically significant difference. . DÀI RỄ TRÊN MOI TRUỜNG CÓ NAA Analysis of variance Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level Between groups 462.86420 5 92.572840 187.280 .0000 Within groups 77.11111 156 .494302 Total (corrected) 539.97531 161 0 missing value(s) have been excluded. Multiple range analysis for DRNAA.DAIRE by DRNAA.NT Method: 95 Percent LSD Level Count Average Homogeneous Groups 6 27 1.1111111 X 5 27 2.4444444 X 1 27 4.1851852 X 2 27 5.2222222 X 3 27 5.4814815 XX 4 27 5.6296296 X contrast difference +/- limits 1 - 2 -1.03704 0.37806 * 1 - 3 -1.29630 0.37806 * 1 - 4 -1.44444 0.37806 * 1 - 5 1.74074 0.37806 * 1 - 6 3.07407 0.37806 * 2 - 3 -0.25926 0.37806 2 - 4 -0.40741 0.37806 * 2 - 5 2.77778 0.37806 * 2 - 6 4.11111 0.37806 * 3 - 4 -0.14815 0.37806 3 - 5 3.03704 0.37806 * 3 - 6 4.37037 0.37806 * 4 - 5 3.18519 0.37806 * 4 - 6 4.51852 0.37806 * 5 - 6 1.33333 0.37806 * * denotes a statistically significant difference. . 5. XỬ LÝ SỐ LIỆU CÂY TRUỜNG XUÂN HOA IN VITRO TRÊN MÔI TRƯỜNG CHỨA IAA CAO CÂY TRÊN MOI TRUƠNG CÓ IAA Analysis of variance Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level Between groups 560.9938 5 112.19877 2.637 .0256 Within groups 6638.3704 156 42.55366 Total (corrected) 7199.3642 161 0 missing value(s) have been excluded. Multiple range analysis for CCIAA.CAOCAY by CCIAA.NT Method: 95 Percent LSD Level Count Average Homogeneous Groups 6 27 46.333333 X 5 27 48.740741 XX 4 27 49.407407 XX 3 27 50.407407 X 2 27 51.518519 X 1 27 51.851852 X contrast difference limits 1 - 2 2.44444 3.50775 1 - 3 1.44444 3.50775 1 - 4 0.33333 3.50775 1 - 5 3.11111 3.50775 1 - 6 5.51852 3.50775 * 2 - 3 -1.00000 3.50775 2 - 4 -2.11111 3.50775 2 - 5 0.66667 3.50775 2 - 6 3.07407 3.50775 3 - 4 -1.11111 3.50775 3 - 5 1.66667 3.50775 3 - 6 4.07407 3.50775 * 4 - 5 2.77778 3.50775 4 - 6 5.18519 3.50775 * 5 - 6 2.40741 3.50775 * denotes a statistically significant difference * denotes a statistically significant difference. SỐ RỄ TRÊN MOI TRUƠNG CÓ IAA One-Way Analysis of Variance Data: SRIAA.SORE Level codes: SRIAA.NT Labels: Means plot: LSD Confidence level: 95 Range test: LSD Analysis of variance Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level Between groups 191.30864 5 38.261728 104.242 .0000 Within groups 57.25926 156 .367047 Total (corrected) 248.56790 161 0 missing value(s) have been excluded. Multiple range analysis for SRIAA.SORE by SRIAA.NT Method: 95 Percent LSD Level Count Average Homogeneous Groups 6 27 2.5925926 X 5 27 2.6666667 XX 1 27 2.9259259 X 3 27 4.0370370 X 2 27 4.2962963 X 4 27 5.6296296 X contrast difference limits 1 - 2 -1.37037 0.32578 * 1 - 3 -1.11111 0.32578 * 1 - 4 -2.70370 0.32578 * 1 - 5 0.25926 0.32578 1 - 6 0.33333 0.32578 * 2 - 3 0.25926 0.32578 2 - 4 -1.33333 0.32578 * 2 - 5 1.62963 0.32578 * 2 - 6 1.70370 0.32578 * 3 - 4 -1.59259 0.32578 * 3 - 5 1.37037 0.32578 * 3 - 6 1.44444 0.32578 * 4 - 5 2.96296 0.32578 * 4 - 6 3.03704 0.32578 * 5 - 6 0.07407 0.32578 * denotes a statistically significant difference. DÀI RỄ TRÊN MOI TRUỜNG CÓ IAA Analysis of variance Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level Between groups 88.938272 5 17.787654 41.902 .0000 Within groups 66.222222 156 .424501 Total (corrected) 155.16049 161 0 missing value(s) have been excluded. Multiple range analysis for DRIAA.DAIRE by DRIAA.NT Method: 95 Percent LSD Level Count Average Homogeneous Groups 6 27 3.7777778 X 1 27 4.1851852 X 5 27 4.6666667 X 2 27 4.8148148 X 3 27 5.6666667 X 4 27 5.8518519 X contrast difference +/- limits 1 - 2 -0.62963 0.35035 * 1 - 3 -1.48148 0.35035 * 1 - 4 -1.66667 0.35035 * 1 - 5 -0.48148 0.35035 * 1 - 6 0.40741 0.35035 * 2 - 3 -0.85185 0.35035 * 2 - 4 -1.03704 0.35035 * 2 - 5 0.14815 0.35035 2 - 6 1.03704 0.35035 * 3 - 4 -0.18519 0.35035 3 - 5 1.00000 0.35035 * 3 - 6 1.88889 0.35035 * 4 - 5 1.18519 0.35035 * 4 - 6 2.07407 0.35035 * 5 - 6 0.88889 0.35035 * * denotes a statistically significant difference. PHỤ LỤC 3 Khối lƣợng của cây Trƣờng xuân hoa trƣớc và sau khi đông khô Hàm lượng nước trong mẫu cây tươi = (0,683 - 0,088)*100/0,683 =87,1% 0,6 g vật liệu tươi = 0,6*(100 – 87,1)/100 = 0,0774 (g) =77,4 (mg) chất khô x (mg) alkaloid/ 0,6 (g) vật liệu tươi = x*100/ 77,4(mg) alkaloid/100 mg chất khô PHỤ LỤC 4 1. Kết quả chất chuẩn catharanthine (20mg/l) khi phân tích bằng CE Trọng lượng tươi Trọng lượng khô 0,5287 0,0673 0,5202 0,1117 0,4794 0,0589 0,5394 0,0645 0,4653 0,0558 0,471 0,064 1,043 0,1259 0,8399 0,1006 0,7234 0,0937 0,5919 0,0797 0,8955 0,1154 1,10151 0,119 Trung bình 0,6832675 0,088041667 2. Kết quả chất chuẩn vindoline (20mg/l) khi phân tích bằng CE 3. Kết quả của mẫu Trƣờng xuân hoa khô khi phân tích bằng CE 4. Kết quả CE phân tích mẫu Trƣờng xuân hoa tƣơi 5. Kết quả CE phân tích mẫu Trƣờng xuân hoa in vitro 6. Kết quả CE phân tích mẫu Trƣờng xuân hoa in vivo 7. Sắc kí đồ CE phân tích mẫu Trƣờng xuân hoa 7 ngày tuổi 8. Sắc kí đồ CE phân tích mẫu Trƣờng xuân hoa 14 ngày tuổi 9. Sắc kí đồ CE phân tích mẫu Trƣờng xuân hoa 21 ngày tuổi 10. Sắc kí đồ CE phân tích mẫu Trƣờng xuân hoa 28 ngày tuổi . 1.00000 0. 291 29 * 1 - 6 2.2 592 6 0. 291 29 * 2 - 3 -1.185 19 0. 291 29 * 2 - 4 -0.66667 0. 291 29 * 2 - 5 1.07407 0. 291 29 * 2 - 6 2.33333 0. 291 29 * 3 - 4 0.51852 0. 291 29 * 3 - 5 2.2 592 6 0. 291 29 * 3. 1 .92 592 59 X 1 27 2 .92 592 59 X 2 27 3.0000000 X 4 27 3.6666667 X 3 27 4.1851852 X contrast difference limits 1 - 2 -0.07407 0. 291 29 1 - 3 -1.2 592 6 0. 291 29 * 1 - 4 -0.74074 0. 291 29. 0,1117 0,4 794 0,05 89 0,5 394 0,0645 0,4653 0,0558 0,471 0,064 1,043 0,12 59 0,8 399 0,1006 0,7234 0, 093 7 0, 591 9 0,0 797 0, 895 5 0,1154 1,10151 0,1 19 Trung bình