1 Carbon Nanotube Biosensor for the Assessment of Personal Quality of Life Katsuyuki Murata 1 , Masuhiro Abe 2 , Yasuo Ifuku 3 , Atsuhiko Kojima 4 , Mitsuaki Shimuzu 5 , Masatoshi Maeda 6 , Tatsuaki Ataka 1 , Kazuhiko Matsumoto 7 1 Olympus Corpration, CREST-JST and NEDO, 2 NEDO, 3 Mitsubishi Kagaku Iatron, 4 Mitsubishi Chemical, 5 AIST, 6 Tsukuba University, 7 NEDO, Osaku University, CREST-JST, and AIST 2 Prolegomenon Quotation from our HP • Future Creation Laboratory of Olympus Corporation – Our goal: enhancement quality of life (QOL) on mind and body – The Future Creation Laboratory is committed to pursuing a variety of research projects aimed at identifying and creating future values for enriching people's lives. 3 Our researches The Future Creation Laboratory conducts highly original research in "HIKARI"-HI Technology, primarily focusing on four areas:Bioscience, Humanware, Optics and Nanotechnology and Ubiquitous energy. Closely linked, these fields will yield new discoveries and values for the future. Approaches taken from four research domains `We explore what future generations want and what universal values will be like in the future.` 4 Bioscience domain -Enhance QOL on mind and body- Our future dreams and goal In the area of carbon nanotube (CNT) biosensors research, we are attempting to establish a technology that allows the ultrasensitive measuring (so called “single molecule detection”) of biomolecules by applying the CNT single electron transistor to biosensors. This technology will open a new possibility for home physical check- ups and will contribute to preventive medicine and health management during recovery. 5 Introduction What is Personal QOL This project is “Research and Development of Nanodevices for Practical Utilization of Nanotechnology”in NEDO 6 Challenge to nanotechnology of Olympus 1980 1990 2000 Reinforcement of core competence A challenge and development to a frontier 2010 Nano measurement Nano medical device Many functions Capsule Precisely micro Micro machine capsule endoscope ＡＦＭcantilever biolever ＭＥＭＳ scanner Nanobio living body imitation sensor end microscope 7 ＷｅｂＰＤＦ file ；21C US security in the 21st centry Current Japan is super aged society Japan → World Country of current aged society: about 10 20 years past: about 20 We take the fastest problem of aging speed underhand 8 Personal QOL system ～Aims to reduce medical cost and promote healthy intentions～ market size 10trillion yen in Japan 200-300 trillion yen in the world Preventive medicine Diagnosis Treatment Postoperative care current future Personal QOL system •Feature •24 hours monitor with a terminal in home •Biotip, microTAS at home at hospital at home 9 24 hours monitor in home server at home network 72 09 ： 10 wearable biosensor Biotip capsule type biosensor PQOL system Central server Medical care and welfare service institution 10 抗体 カーボンナノチューブ ソース ドレイン 抗体 カーボンナノチューブ ソース ドレイン CNT Source Drain Target molecule CNT-FET Bioaffinity imaging MicroTAS 中央サーバ Cupcel diagnosis tip Network DNA direct reading NEW Biotip Biosensor array Enhancement of QOL (at-home distant place medical care) PQOL system Claim success for introducing new measurement system SPM for Bio Social value Economic value Economic value 72 09 ： 10 72 09 ： 10 [...]... Sensitivity 15 CNT biosensor of Amperometry or Potensiometry Target molecules antibody 抗体 antibody 抗体 CNT カーボンナノチューブ CNT Source ソース Amperometry （Large surface area electrode） Drain ドレイン Potensiometry （FET） 16 Previous CNT-FET biosensor research DNA Hybridization Target DNA Probe PNA Au Si substrate SiO2 V CNT A We achieved detection of 6.8 fmol/L of DNA using a CNT-FET biosensor 17 Biosensor array Target... マルチプレクサー specimen injection pretreatment Drain ドレイン 多項目アレイ マルチプレクサー microTAS 18 Why did we select a CNT biosensor for PQOL system? • PQOL system requires – wearable biosensor • small and light • microTAS – real time sensing – non disposable 19 CNT biosensor compared with conventional protein sensing methods CNT Biosensor Sensitivity Chromatography Chemical luminescence c.a 1μmol/l nmol/l order most high sensitivity... by MEMS technology 12 Position of CNT biosensors in CNT applications Mass-production method of CNTs must be established Hydrogen storage Catalyst Electrode of Fuel cell Capacitor Filed emission DDS FET STM tip CNTs not required in large quantities 13 Contents of this presentation • What is CNT biosensor? • Problems for practical use and solutions 14 What is CNT -biosensor? • We studied – Amperometry... Results: top gate is best structure • CNT must be kept clean for optimal CNTFET performance • Top-gate CNT-FET biosensor – CNT kept clean because CNT is covered with SiNx 27 Top-gate CNT-FET illustration Resin Top Gate (Au/Ti) 10000 mm2 SiNx Drain(AU/Ti) SiO2 Si Source (Au/Ti) metal cat (Co) Carbon nanotube 28 Top-gate structure prevents adsorbing contamination on CNT Water, oxygen and other contaminates... device 31 Top-gate CNT biosensor has low sensitivity 32 Why low sensitivity? • No modulation by schottky barrier Schottky barrier Top-gate FET was modulated by only channel part, back gate FET was modulated by schottky barrier and channel part 33 Double gate structure enhances CNT-FET sensitivity resin top-gate CNT-FET Top Gate SiNx Drain Source SiO2 Metal cat Si Back Gate Carbon nanotube 34 Double gate... Olympus, AIST, Mitsubishi Kagaku Iatron, & Osaka Univ • PQOL system using CNT -Biosensor NEDO project: Olympus, Osaka Univ & JAIST 11 Relationship between JST & NEDO Project • CREST-JST Project • NEDO Project • Mainly fundamental research • Investigation for practical use – CNT-field effect transistor (FET) – using CNT-FET in biosensor – measure yield, , quantifiability, selectivity, sensitivity, stability,... 1μmol/l nmol/l order most high sensitivity conventional study Mobility yes yes no sensor array yes no yes Real time sensing yes no no Not disporsable? yes no no 20 Problems for practical use of CNTFET based biosensor • CREST-JST – Fundamental design – Stability & reliability • NEDO – Quantifiability – Sensitivity – Yield – Selectivity 21 Contamination provides less stability of CNT-FET Washing CNT using aprotic... nucleophilicity（basic） 24 Experiments and sample preparation • Au/Ti Electrode Photolithography • CVD • • H2 ５００ｓｃｃｍ • • EtOH ７５０ｓｃｃｍ ＠９００℃ １０min Wash by DMAC CVD process contaminates the CNT with pitch, amorphous carbons and so on Photolithography process makes lots of photoresist residue 25 Vd-Id Characteristic of CNT-FET Before and After Washing As Prep Drain Current I DS/A 5 10 0 1.5 10-6 -7 -5 10 -6 -1 10 -6 . 1 Carbon Nanotube Biosensor for the Assessment of Personal Quality of Life Katsuyuki Murata 1 , Masuhiro. future.` 4 Bioscience domain -Enhance QOL on mind and body- Our future dreams and goal In the area of carbon nanotube (CNT) biosensors research, we are attempting to establish a technology that allows the ultrasensitive. substrate SiO 2 We achieved detection of 6.8 fmol/L of DNA using a CNT-FET biosensor Previous CNT-FET biosensor research 18 microTAS Biosensor array マルチプレクサー マルチプレクサー 多項目アレイ 抗体 カーボンナノチューブ ソース ドレイン 抗体 Potentiometry atnibody Target