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Beauveria bassiana
3/18/2011 Sustainable i S t i bl insect management: t t Experiences in development of a Canadian biopesticide (Metarhizium anisopliae) Dan Johnson Professor of E i P f f Environmental Science, t lS i Canada Research Chair in Sustainable Grassland Ecosystems Water and Environmental Science Building, Department of Geography, University of Lethbridge, Alberta, Canada 3/18/2011 dan.johnson@uleth.ca Water and Environmental Science Building University of Lethbridge, Alberta, Canada We are currently working toward licensing, in cooperation with Office of Research Services, and Health Canada PMRA 3/18/2011 Metarhizium anisopliae (photos D Johnson, 2010) 3/18/2011 dan.johnson@uleth.ca 3/18/2011 Other insects 3/18/2011 dan.johnson@uleth.ca 100 Mortality (%) 80 60 IMI 330189 S54 40 20 Days Cumulative mortality of field-collected grasshoppers treated with Metarhizium anisopliae var acridum IMI 330189 and M anisopliae var anisopliae S54 var S54 Entz, S.C., Kawchuk, L.M and Johnson, D.L 2008 Discovery of a North American genetic variant of the entomopathogenic fungus Metarhizium anisopliae var anisopliae pathogenic to grasshoppers BioControl 53(2): 327-339 3/18/2011 Production on grain: optimizing temperature, nutrients and cycle time 3/18/2011 3/18/2011 dan.johnson@uleth.ca We adapted our application technology to the applications methods currently in use by land managers and farmers farmers 3/18/2011 Metarhizium anisopliae Plate sample from field during spray application 10 3/18/2011 0.25 sq m sampling ring 11 3/18/2011 Trichomalopsis sarcophagae (received from Dr K Floate) Safe for candidate biocontrol agents of nearby agricultural systems 12 3/18/2011 100 90 80 % Mo rtality 70 60 T80 (C ontrol) 10^4 s pore 50 10^5s pore Trichomalopsis sarcophagae 10^6s pore 40 10^7s pore 30 20 10 10 11 12 13 D ay 13 3/18/2011 dan.johnson@uleth.ca Replicated experiments showed no impact on rainbow trout trout, crayfish or earthworms More detailed histopathology tests are in progress In cooperation with Lethbridge College Aquaculture Center of Excellence 14 3/18/2011 dan.johnson@uleth.ca Chaoborus americanus 0, 1.5x104, 3.0x104, or 1.2x105 fungal spores per ml, of Metarhizium anisopliae var anisopliae isolate S54 Negligible mortalities in replicated treated or control jars 15 3/18/2011 Funding and cooperation: AAFC Pesticide Risk Reduction Program, Pest Management Centre Alberta Crop Industry Development Fund Canada Foundation for Innovation Canada Research Chairs Department of National Defence, Canada (environmentally sustainable airfield insect control to reduce birdstrikes) NSERC Pulse Canada Saskatchewan Pulse Growers 16