[...]... selective nuclear protein import J Cell Biol 2002; 158:6 3-7 7 24 Nuclear Import and Export in Plants and Animals 157 Sukegawa J, Blobel G A nuclear pore complex protein that contains zinc finger motifs, binds DNA, and faces the nucleoplasm Cell 1993; 72:2 9-3 8 158 Shah S, Tugendreich S, Forbes D Major binding sites for the nuclear import receptor are the internal nucleoporin Nup153 and the adjacent nuclear. .. NPCs within the NE, and specifically in importin-mediated transport.165 Like Nup153, Tpr has binding sites to importin β, and binds importin α/β complexes in vitro.158 In Xenopus egg extracts this binding is released by GMP-PNP, a nonhydrolyzable analog of GTP Unlike Nup153, however, Tpr cannot bind importin α/β when the latter are complexed to NLS-bearing cargo Microinjection of anti-Tpr antibodies to... protein-interaction domains In vitro it interacts with poly(G) and poly(U) RNAs, as well as with importin α/β and transportin Fragments of the protein containing these docking sites acted as dominant-negative inhibitors of the respective import pathways, when added in excess to in vitro import assays.158 Immunodepletion of Nup153 from Xenopus egg extracts implicate the protein in immobilizing NPCs within... WC et al Importin beta is a mitotic target of the small GTPase Ran in spindle assembly Cell 2001; 104:9 5-1 06 56 Gruss OJ, Carazo-Salas RE, Schatz CA et al Ran induces spindle assembly by reversing the inhibitory effect of importin alpha on TPX2 activity Cell 2001; 104:8 3-9 3 57 Fagotto F, Gluck U, Gumbiner BM Nuclear localization signal-independent and importin/ karyopherin-independent nuclear import. .. of the pore itself on one hand, 8-1 1 or entirely biochemical sieves on the other.1 2-1 5 While deep modulation of calcium levels has a Nuclear Import and Export in Plants and Animals, edited by Tzvi Tzfira and Vitaly Citovsky ©2005 Eurekah.com and Kluwer Academic / Plenum Publishers 2 Nuclear Import and Export in Plants and Animals pronounced effect on nuclear pore structure in vitro,11,16 calcium depletion... responsible for intracellular transport mediated by the nuclear export signal Nature 1997; 390:30 8-1 1 48 Pasquinelli AE, Powers MA, Lund E et al Inhibition of mRNA export in vertebrate cells by nuclear export signal conjugates Proc Natl Acad Sci USA 1997; 94:1439 4-9 20 Nuclear Import and Export in Plants and Animals 49 Kudo NS, Khochbin K, Nishi K et al Molecular cloning and cell cycle-dependent expression... nucleus and its molecular components include packaging and maintaining the integrity of the cellular genetic material, generating messages to the protein synthesis machinery of the cell, assembling ribosome precursors and delivering them to the cell cytoplasm, and many more As a complex machine, the nucleus maintains a constant two-way flow of information with the surrounding cytoplasm, such as import and. .. the nuclear pore complex induced by depletion of nuclear Ca2+ stores Science 1996; 273:187 5-7 17 Strubing C, Clapham DE Active nuclear import and export is independent of lumenal Ca2+ stores in intact mammalian cells J Gen Physiol 1999; 113:23 9-4 8 18 Wei X, Henke VG, Strubing C et al Real-time imaging of nuclear permeation by EGFP in single intact cells Biophys J 2003; 84:131 7-2 7 19 Moore MS Ran and nuclear. .. of Nup358.153,154 12 Nuclear Import and Export in Plants and Animals Also known as Ran binding protein 2 (RanBP2), Nup358 binds the Ran GTPase activating protein RanGAP and accelerates its promotion of Ran-bound GTP hydrolysis.155 This would be a logical termination step for recycling of importin β-type receptors that should be released from Ran on return to the cytoplasm Targetting of RanGAP to Nup358... Klebe C, Bischoff FR, Ponstingl H et al Interaction of the nuclear GTP-binding protein Ran with its regulatory proteins RCC1 and RanGAP1 Biochemistry 1995; 34:63 9-4 7 142 Kutay U, Izaurralde E, Bischoff FR et al Dominant-negative mutants of importin-beta block multiple pathways of import and export through the nuclear pore complex EMBO J 1997; 16:115 3-6 3 143 Cronshaw JM, Krutchinsky AN, Zhang W et al Proteomic . h0" alt="" MOLECULAR BIOLOGY INTELLIGENCE UNIT Tzvi Tzfira and Vitaly Citovsky Nuclear Import and Export in Plants and Animals Nuclear Import and Export in Plants and Animals TZFIRA • CITOVSKY MBIU MOLECULAR. the Host Protein VIP1 92 A Model for T- DNA Nuclear Import and Intranuclear Transport 92 7. Regulation of Nuclear Import and Export of Proteins in Plants and Its Role in Light Signal Transduction. eight staves surrounding a central channel, with inward projections attached to the center of the staves. Bands corre- sponding to the two rings at the top and bottom close the staves. This central