... pT7Blue2T:RS21 -C6( NdeI ⁄ BamHI) andpT7Blue2T:RS21 -C6( NcoI ⁄ BamHI), respectively.Construction of expression plasmidsPlasmids pET3a:RS21 -C6, pET32a(+):RS21 -C6, pcDNA3.1-hyg(+):RS21 -C6 and pIRES2-EGFP:RS21 -C6 ... 17.4 (kDa) pcDNA pcDNA :RS21 -C6 pIRES2-EGFP pIRES2-EGFP :RS21 -C6 20 15 (kDa) RS21 -C6 GAPDH RS21 -C6 GAPDH 31.3 47.3 84.7 114 W N Mt Cy t RS21 -C6 Lamin B HSP60 GAPDH 0 5 ... FEBSTrxA-RS21 -C6 protein was expressed frompET32a(+):RS21 -C6 and was used as an antigen toprepare anti-RS21 -C6 rabbit serum. Western blot anal-ysis, using affinity-purified anti-RS21 -C6 Ig, showedthat...