... Lanes h and m, ATnI-52K; lanesi and n, ATnI -19 K; lanes j and o, ATnI 1) 128; lanes k and p, ATnI 13 0)252; lanes l and q, ATnI232)292; lane r, Akazara scallop TnC. Complex forma-tion was detected ... and TnC activated the ATPase in a Ca2+-dependentmanner almost as effectively as intact Akazara scallop troponin. Therefore,Akazara scallop troponin regulates the contraction through the activatingmechanisms ... TnI-fragment and native TnC and TnT weremixed at a 1 : 1 : 1 molar ratio and dialyzed against 6 murea, 0.5 m KCl, 10 mm Tris ⁄ HCl (pH 7.6), and 5 mm2-mercaptoethanol. The urea and KCl concentrations...