... species was detected with g values at 2.031,
1.994, and 1.951. The resonance started to develop at
potentials ‡ 0 mV and was stable at potentials up to
+350 mV. The loss and formation of the resonance ... of AF499 was identified as
an archaeal promoter element by seque nce analysis. The sequ ence AAAGGTTAATATA shows a high le vel of identity with th e consensus se quence
()...
... bands from SDS ⁄
PAGE was performed as described by Bamford et al. [22].
MS
MALDI-TOF MS was performed using an Ultraflex TOF ⁄
TOF instrument (Bruker Daltonik, Bremen, Germany). Pro-
teins and ... minimal med-
ium [19] with 2% lactose as a carbon source, 1% potassium
phthalate as a buffering agent (pH 5.5), 0.1 mm CuSO
4
and
1% tyrosine as an indicator substrate was used. The tra...
... concentrations
less than 25 m
M
. However, N-acetyl derivatives, namely
N-acetylglucosamine (GlcNAc), N-acetylgalactosamine
(GalNAc) and N-acetyl mannosamine (ManNAc) were
better inhibitors than their ... animal glycoconjugate. They act
as an important component of the ligands recognized by the
lectins. Recognition can be affected by specific structural
variations and modifications of...
... intermediate of
Fig. 5. Putative genomic organization of the human phosphohistidine phosphatase gene. Astandard
BLAST
search, with AF164795 as the query, was
performed against the nonredundant databases ... PROCEDURES
Materials
Phosphoamidate was prepared according to the method of
Wei and Matthews [4]. Suc-Ala-His-Pro-Phe-pNA was
purchased from Bachem AG, Switzerland. Malachite gre...
... amount of Bip-Pro in the extracellular uptake medium
was quantified according to the laboratory standard HPLC
(La-Chrom
Ò
; Merck-Hitachi, Darmstadt, Germany) with a
diode array detector and a ... inves-
tigation because of their physiological importance and
their pharmaceutical relevance as drug carriers [1–6].
Both transporters catalyse the uptake of most dipep-
tides and t...
... SmRXR1 can interact with mammalian coac-
tivators of transcription, and S. mansoni coactivators
of transcription may have a similar mechanism to
SmNR1 ⁄ SmRXR1. Recently four NR coactivators,
SmGCN5, ... 13 and 23 contain nonspecific competitor. Lanes 4, 14 and 24 contain DR0 as
competitor. Lanes 5, 15 and 25 contain DR1 as competitor. Lanes 6, 16 and 26 contain DR2 as compet...
... treated with dithiothreitol (5 mm) to reduce
the disulfides, and assayed as above. Thiols were quanti-
fied by comparison of peak areas to standard curves of
those standards. Coenzyme A was measured ... the
ratio of protein concentration to flavin concentration
(as determined at 460 nm). blast and tfasta analysis
of the phCoADR revealed a significant level of identity
to...
... Storz, G. &
Rhee, S.G. (1994) Cloning and sequencing of thiol-speci®c
antioxidant from m ammalian brain: alkyl reductases and thiol-
speci®c antioxidant de®ne a large family o f antioxidant ... membrane was exposed to X-ray ® lm at )20 °Cor,
when using an intensifying s creen, at )80 °C.
RNA for Northern analyses was isolated as described
above and separated in a 1.3% ag...
... microorga-
nisms, plants and animals and their function and physio-
logical roles have been investigated and identified [12,13].
N-acyl-
D
-amidohydrolase (EC.3.5.1.81, N-D-AAase) is an
enzyme capable ... SDS/PAGE and Western blotting of N-D-AAase. (A) SDS/
PAGE (10% acrylamide). M, Molecular mass standards; lane 1, crude
extract; lane 2, heat treatment of crude extract; lane 3...