... hybridization o feither PstI(lanea)orEcoRI (lane b) digested c hromosomal DN A in c ase of sipV or Hin dIII (lane a) or EcoRI-SacI (lane b) digested genomic DNAin case of sipW. Hybridization indicated ... protein;SPase I, signal peptidase I (leader peptidase I) ; TS, temperaturesensitivity; IPTG, isopropyl thio-b-D-galactoside.De®nitions:SipS(ba), SipS(bj), SipT(ba), SipV(ba) and SipW(ba)arethe ... i ndustrial enzymes[28], little is known a bout physiological and g eneticpeculiarities [29]. In previous studies, two SipS-like signalpeptidases SipS1(ba) and SipS2(ba)ofB. amyloliquefacienswere...