... interactions with Grb2, LAT, PLC-c1andthef-chain of the T cell receptor [14–16]. The SH2 domain ofShb bound the f-chain, the PTB domain bound LAT and the proline-rich regions associate with PLC-c1 and ... SDS/PAGE and the blot was probed with anti-(phospho-JNK) Ig, and after stripping, reprobed with anti-JNK Ig. The positions of Rac1, phospho-JNK and total JNK are indicated in the figures. The relative ... (PLC-c1), 75 and 36 kDa(LAT) was observed and described in Fig. 3, with the relative phosphorylation of these proteins compared to the total amount of SLP-76 in the CD3 treated lanes. The 75-kDa...