... lysates and media. Nonspecificbands may be present and can be eliminated from consideration using the pSVL vectorcontrol lanes for comparison (Fig. 2A,B, lanes 1 and 3). The sizes of specific bandsreported ... polymerase buffer, 2.5 mMMgCl2, 400 µM dNTPs, 200 ng of primers 1 and 2 or 3 and 4, 1 ng of template DNA (withonly primers 3 and 4), and 5 U of Taq polymerase. Perform a PCR program in a suitablethermal ... polymerase, and incubate the reaction at 72˚C to allow extension of the linkedtemplates. Add 2 µg each of primers 5 and 6, and continue the PCR with 30 cycles at 94˚Cfor 30 s, 50˚C for 1 min and 72˚C...