... of the two forms of Plasmodium falciparum acylcarrier protein, the apo and holo forms, were determined by guanidinehydrochloride-induced denaturation. Both the apo form and the holo form displayed ... was almost fullyreversible in both forms of the protein. Removal of theperturbation makes the protein regain its native form. The unfolding reactions of both forms are simple two-state processes, ... denaturation studiesthat the holo form of the protein has greater stabilitythan the apo form. The differences in the unfoldingthermodynamic parameters of the two forms are givenin Tables 1–3....