... by p reincubatingthe enzyme with substrate, succinic semialdehyde, but not b ypreincubating with coenzyme NAD+. After tryptic diges-tion of the enzyme modified with DABIA, two peptidesabsorbing ... acids derivatized with phenylisothiocyanate wereidentified and quantified by HPLC (Waters, Milford, MA,USA) Pico-Tag system, using a Nova-Pak C18column runat room temperature with a flow rate ... Brain succinic semialdehyde dehydrogenaseReactions of sulfhydryl residues connected with catalytic activityByung Ryong Lee1, Dae Won Kim1, Joung-Woo Hong2, Won Sik Eum1,...