... Hence, the dependence of k2 of wild-type and all mutants on the concentration of MgCl2was investigated at 278, 283, 288, 293 and 298 K.Table 1. Kinetic and thermodynamic parameters of the reaction ... reaction steps, not for the interaction of macromolecules in solution. Furthermore, the magnitude(but not the sign) of the thermodynamic parameters of activation measured with the same experimental ... charge (D44A). The plastocyanin -interaction sites of Cyt f and photosystem I therefore appear to have adifference in charge of 2.These conclusions can be drawn from the shape of the data curve...