... models to study HCV.
Int. J. Med. Sci. 2006, 3 30Figure 1. Life cycle of HCV. The steps of the viral life cycle are depicted schematically. The topology of HCV structural and nonstructural proteins ... recently, recombinant infectious HCV could be produced in cell culture, rendering all steps of the viral life cycle, including entry and release of viral particles, amenable to syst...
... Hermoso,J.,Pignol,D.,Kerfelec,B.,Crenon,I.,Chapus ,C. &
Fontecilla-Camps, J .C. (1996) Lipase activation by nonionic
detergents. The crystal structure of the porcine lipase-colipase-
tetraethylene glycol monooctyl ether ... after coexpression of S132T and WWW
mutants. Schematic dimeric structures of LPL
in head-to-tail configuration after cotransfec-
tion of inactive LPL mutants an...
... (CCG
CTCGAGCCTAACGG
TGTGGAATAC, which hybridizes at positions 715–732 in
the tps1
+
ORF and shows an internal XhoIsite),andthe3¢
oligonucleotide TAF-3 (CTACG
GCGGCCGCCCGAGC
TAGAATTCATCGA, which hybridizes at the 3¢ end of
tps1
+
ORF ... oligonucleotide TAG-3 (CTAC
G
GCGGCCGCCATTTTTATGAATGGAAA, which
hybridizes at the 3¢ end of ntp1
+
ORF and incorporates a
NotI site placed immediately ups...
... isolation of CoOMT from
C. japonica ESTs, the expression of functionally active
recombinant enzyme, and its characterization. Characteri-
zation of its specificity showed that Coptis CoOMT could
methylate ... [20,21].
Construction and sequencing of cDNA library
of
C. japonica
Poly(A)
+
RNA was isolated, and a cDNA library was
constructed as described elsewhere [14]. The cDNA
Fig. 1...
... plasmid was named pYCfCTA
1::lacZ. A portion of MRG19 was amplified by PCR using
primers PJB102 (5¢-GACCGTAGGTACCATGTTGGCT
TCAG-3¢) and PJB103 (5¢-CGGGCCCCTC GAGGCCCA
TCATCTAA-3¢) carrying KpnIandXhoI ... Broach, J.R. & Rowe, L.B. (1978) Regulation of
galactose pathway in Saccharomyces cerevisiae: Induction of
uridyl transferase mRNA and dependency on GAL4 gene func-
tion. Proc. Natl...
... (1994)
Phosphorylation of the p34 subunit of human single-stranded-
DNA-binding protein in cyclin A-activated G1 extracts is
catalyzed by cdk-cyclin A complex and DNA-dependent protein
kinase. Proc. Natl Acad. ... j,signalintensityofsuper-
coiled DNA (sc). H, hypoxic.
Fig. 5. Dissociation of minichromosome-bound RPA-34 by excess
competitor single-stranded DNA. Minichromosomes of hypox...
... receptors are members of the type I cytokine
receptor superfamily, w hich is charac terized by the presence
of at least one cytokine-binding homology r egion (CHR)
composed of two fibronectin type ... cytokine receptors, except for hgp130 [51] and
granulocyte colony-stimulating factor receptor [54]. There-
fore, c
c
CHR, the short form of the c
c
ectodomain, may be
Table 2. Double...
... JNK activity, the cells were incubated for 6 h
in th e presence of MEM53 antibody. The luciferase activity was cor-
rected for the efficiency of transfection by determining the ac tivity of
plasmid ... tetraspanin
superfamily: molecular facilitators. FASEB J. 11 , 428–442.
2. Horejsi, V. & Vlcek, C. (1991) Novel structurally distinct family of
leucocyte surface glyco proteins inclu...
... G, Casciani CU. Hepatitis C virus infection in Italian kidney graft recipients. Changing risk factors and hepatitis C virus genotypes. Ital J Gastroenterology Hepatology 1997; 29:448-55. 67. Cheung ... Non-A Non-B Hepatitis Research Group. Effect of screening for hepatitis C virus antibody and hepatitis B core antibody on incidence of post-transfusion hepatitis. Lan...
... hepatitis C, liver fibrosis, cirrhosis, hepatocellular carcinoma, HCV, HCC 1. Introduction Chronic hepatitis C is the most common cause of chronic liver disease and cirrhosis, and the most common ... rate of chronic HCV infection than Caucasians and Hispanic whites. In prospective surveys among inner-city Baltimore (Maryland, U.S.) injection drug users, the prevalence of chronic...