... added, and the mixture was incubated at 4 °C
for 1 h with intermittent mixing. The mixture was subse-
quently centrifuged at 100 g for 60 s to pellet the bound
protein with the resin. The supernatant ... polymerase, with sense and antisense
primers (marked with the prefix E, Table 2) corresponding
to the N- and C-termini for each deletion mutant protein
and...
... Fed Up With the
Legal System sets out an agenda for legal reform and renewal. It
comprises a series of practical proposals for making the American
legal system more understandable, affordable and ... procedures. They have streamlined
procedures and encourage people to handle probate without a
lawyer. California and some other states have created fill-in -the-
blank forms...
... on whether such findings hold for
LMICs and MHICs. Therefore, we split the global data into
LMICs and MHICs and carried out the same analysis sep-
arately for each of those income groups. The importance
of ... significantly associated with lower MMR
for the two income groups. Female literacy is notably related to lower IMR and U5MR for both income groups;
and only...
... E6
cells were infected with a part of the supernatant and
again new supernatant was collected and used in infection
(for details, see Methods). Altogether 10 passages were
performed. Total RNA was ... TULV/Lodz
and TULV/Moravia isolates. The supernatant was col-
lected and used to infect fresh cells. Altogether 6 passages
were performed and the RNA was analyzed by RT-PCR
a...
... replication
Pol e is one of the three major replicases in the eukary-
otic cell [22]. Therefore, we tested the effect of the
replicative state of the cell on the interaction between
Pol e and RNA pol II. ... medium and
were then permeabilized with 0Æ2% Triton X-100, fixed with
3% paraformaldehyde and quenched with 50 mm NH
4
Cl
(all solutions prepared in NaCl ⁄ P
i
),...
... level set-
up. These wave effects are significant near the
landfall area and are affected by the process that
typhoon approaches the coastline.
In the 1960s, the theory of wave setup were
developed ... test the
theory and prove its reliability throughout
simulating the wave crashed onto the shore [3].
Moreover, there was a high correspondence
between Longuet-Higgi...
... carefully
controlled and monitored.
NEW MOTHERS AND MOTHERS-TO-BE
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New Mothers 15/5/03 11:24 AM Page 34
reaction is likely to be to forget all this and binge—to
make up for ... cope with life’s little ups and
downs, will shake the foundations of any weight-loss
program to the point where over-eating and consequent
weight gain—become...