... proton catalysis
performed by the distal histidine residue (Eqn 4).
Similarly, Shaanan [31] reported the stereochemistry of
the iron-dioxygen bond in human HbO
2
by single-crystal
X-ray analysis. In ... autoxidation reaction of HbO
2
,aswellasMbO
2
,
pH can affect the rate in many d ifferent ways. Recent kinetic
and thermodynamic studies of the stability of mammalian
oxymyoglobins h...
... purified by
poly(ethylene glycol) precipitation. Of these, 200 clones were
selected arbitrarily for sequencing to verify the randomiza-
tion of the library.
Phage-displayed pentapeptide library screening
This ... discovery of new biological substrates.
A system using a monovalent phage library capable of
displaying several million different substrates, which
enabled simultaneous testin...
... Key
Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua
University, Beijing, China;
3
Protein Science Laboratory of the Ministry ... pathways involving the formation of distinct
transition intermediates [1–3,7,8]. The complexity of the
multiple pathways means that different mechanisms may
occur with different types of...