... and cer-tain interleukins play a key-role in initiating systemic inflammatory response syndrome (SIRS) and it is known that vitamin D may modulate the cytokine ex-pression from the monocytes and ... pre-treated with daily SC in- jections of 1,25-dihydroxy-vitamin D3 100 ng/kg or placebo vehicle for 3 days. In study 1, sepsis was accom-plished by abdominal surgery comprising a coe...
... per-formed by adopting the gating strategy defined by the International Society of Haematotherapy and Graft Engineering (ISHAGE) guidelines [27]. The method of the selection of stem/progenitor cells ... improvement of CD34+ cells in circulation during dieting program was 69% ± 50%. In conclusion, the weight loss program analyzed in our study resulted in the...
... extract and p-synephrine [8-11]. The safety of p-synephrine is clouded by its structural similarity to nor-epinephrine in spite of the fact that the pharma-cokinetics of the two compounds ... properties of p-synephrine (hydroxyl group on the para position of the benzene ring) which is found in C. aurantium, and m-synephrine (phe-nylephrine; with the hyd...
... eliminated by the rate-limiting enzyme, dihydropyrimidine dehy-drogenase (DPD). The DPD activity is found in most tissues, but is highest in the liver. The activity of DPD of diurnally active cancer patients ... thereafter every 3 months for 3 years by endoscopy and CT scan. After 3 years, patients were seen every 6 months. During the follow-up period, a routine course of physical...
... University. Drugs Navelbine® Injection (VNR) and Adriacin® Injec-tion (DXR) were kindly provided by Kyowa Hakko Kirin Co. (Tokyo, Japan). A 10 mg/mL vial of VNR was diluted with normal saline (Otsuka ... out of 8 animals. In addition, there was inflammatory cell infiltration (Grades 1-3) in the proximal part of the vein in all 8 animals and in the distal part of the vein in...
... invade erythrocytes in the presence of 1- 0.1 – 0.01 and 0.001μg/ml anti-CSP monoclonal antibody (mAb). The number of ring stage infected erythrocytes of 3 replicates was counted. Efficacy of ... was maintained in culture as described by Trager and Jensen [8]. Cultures were synchronised by two rounds of sedimentation using gelatine. Briefly, cultures of approximately 5% par...
... of the protein without
seriously affecting the substrate binding affinity.
Binding analyses using BIAcore system
To examine whether the mutation of Gly10 to Leu or
Pro affects the substrate binding ... for Tk-G10S.
Binding analysis of proteins to RNA ⁄ DNA hybrid
Binding of the proteins to the substrate was analyzed using
the Biacore X instrument (Biacore, Uppsala, Sweden).
Twenty-nine...
... states, indica-
ting changes in the internal dynamics and domain orienta-
tion of myosin. In the AdoPP[CH
2
]P state, approximately
half of the myosin heads reflected the motional state of
ADP–myosin, ... methods.
Orientation of probe molecules
Myosin in fibers was spin-labeled with an isothiocyanate-
basedspinlabel,whichisbelievedtobindtothefast
reacting thiol sites in the cata...
... retinol binding proteins where it has been
suggested that these binding proteins may act, not only as
buffers or reservoirs of intracellular retinoids to maintain
significant levels of free retinoids, ... Roles of cellular retinol-
binding protein and cellular retinoic acid-binding protein in the
metabolic channeling of retinoids. In Retinoids. Progress in
Research and Clinical A...
... Fasano
3
1
Department of Chemistry ‘IFM’, University of Torino, Italy;
2
Department of Biology, University ‘Roma Tre’, Rome, Italy;
3
Department of
Structural and Functional Biology, University of Insubria, Italy
Haem ... the N-form [1,3,4,14,35,36]. Haem inhibits
drug binding, possibly stabilizing the N-species of HSA
(present study). Accordingly, binding of ibuprofen and
warfa...